Table S1: Summary of genetic relationships and social groups of wild caught Botswana cheetahs.
ID no
Location
caught
Sex
Age
(months)
Social groupa
# 1b
#5
#6
#7
#8
#9
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
F
M
F
M
F
M
> 30 < 48
> 6 < 12
> 6 < 12
> 6 < 12
> 6 < 12
> 6 < 12
# 22b
# 17
# 18
# 19
# 20
# 21
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
F
M
M
F
M
M
# 29b
# 28
# 30
Jwaneng
Jwaneng
Jwaneng
# 33b
# 49
# 50
# 51
# 52
# 37b
# 34
# 35
# 36
# 38
Genetic relationships
Family 1
Family 1
Family 1
Family 1
Family 1
Family 1
Social
group
genetically
related
Yes
Yes
Yes
Yes
Yes
Yes
> 48 < 96
> 12 < 18
> 12 < 18
> 12 < 18
> 12 < 18
> 12 < 18
Family 2
Family 2
Family 2
Family 2
Family 2
Family 2
Yes
Yes
Yes
Yes
Yes
Yes
Mother of # 17, 18, 19, 20, 21
Sibling of # 18, 19, 20, 21 / Offspring of
Sibling of # 17, 19, 20, 21 / Offspring of
Sibling of # 17, 18, 20, 21 / Offspring of
Sibling of # 17, 18, 19, 21 / Offspring of
Sibling of # 17, 18, 19, 20 / Offspring of
F
F
F
> 48 < 96
> 12 < 18
> 12 < 18
Family 3
Family 3
Family 3
Yes
Yes
Yes
Mother of # 28, 30
Sibling of # 30 / Offspring of # 29
Sibling of # 28 / Offspring of # 29
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
F
F
M
M
F
> 30 < 48
> 12 < 18
> 12 < 18
> 12 < 18
> 12 < 18
Family 4
Family 4
Family 4
Family 4
Family 4
Yes
Yes
Yes
Yes
Yes
Mother of # 49, 50, 51, 52
Sibling of # 50, 51, 52 / Offspring of
Sibling of # 49, 51, 52 / Offspring of
Sibling of # 49, 50, 52 / Offspring of
Sibling of # 49, 50, 51 / Offspring of
# 33
# 33
# 33
# 33
1.000
1.000
1.000
1.000
1.000
Jwaneng
Jwaneng
Jwaneng
Jwaneng
Jwaneng
F
M
M
F
F
> 48 < 96
>0<6
>0<6
>0<6
>0<6
Family 5
Family 5
Family 5
Family 5
Family 5
Yes
Yes
Yes
Yes
Yes
Mother of # 34, 35, 36, 38
Sibling of # 35, 36, 38 / Offspring of
Sibling of # 34, 36, 38 / Offspring of
Sibling of # 34, 35, 38 / Offspring of
Sibling of # 34, 35, 36 / Offspring of
# 37
# 37
# 37
# 37
1.000
1.000
1.000
1.000
1.000
Mother of # 5, 6, 7, 8, 9
Sibling of # 6, 7, 8, 9 / Offspring of
Sibling of # 5, 7, 8, 9 / Offspring of
Sibling of # 5, 6, 8, 9 / Offspring of
Sibling of # 5, 6, 7, 9 / Offspring of
Sibling of # 5, 6, 7, 8 / Offspring of
Genetic
probabilityc
1.000
1.000
1.000
1.000
1.000
1.000
#1
#1
#1
#1
#1
# 22
# 22
# 22
# 22
# 22
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
# 39b
# 41
# 42
Ghanzi
Ghanzi
Ghanzi
F
F
F
> 48 < 96
> 18 < 30
> 18 < 30
Family 6
Family 6
Family 6
Yes
Yes
Yes
Mother of # 41, 42
Sibling of # 42 / Offspring of # 39 and 43
Sibling of # 41 / Offspring of # 39 and 43
# 58b
# 55
# 56
# 57
# 59
# 60
Ghanzi
Ghanzi
Ghanzi
Ghanzi
Ghanzi
Ghanzi
F
M
M
F
F
M
> 48 < 96
> 6 < 12
> 6 < 12
> 6 < 12
> 6 < 12
> 6 < 12
Family 7
Family 7
Family 7
Family 7
Family 7
Family 7
Yes
Yes
Yes
Yes
Yes
Yes
Mother of # 55, 56, 57, 59, 60
Sibling of # 56, 57, 59, 60 / Offspring of
Sibling of # 55, 57, 59, 60 / Offspring of
Sibling of # 55, 56, 59, 60 / Offspring of
Sibling of # 55, 56, 57, 60 / Offspring of
Sibling of # 55, 56, 57, 59 / Offspring of
# C10b
# C1
Moremi GR
Moremi GR
F
F
> 48 < 96
> 18 < 30
Family 8
Family 8
Yes
Yes
Mother of # C1
Offspring of # C10
1.000
1.000
# 64b
WD7b
# 23
# 26b
# 27
Ghanzi
Ghanzi
Ghanzi
Ghanzi
Ghanzi
F
M
M
M
M
> 48 < 96
> 12 < 18
>0<6
> 6 < 12
> 48 < 96
Lone 1
Lone 2
Lone 3
Lone 4
Lone 5
Yes
Yes
N/A
Yes
N/A
# 40
# 43b
Ghanzi
Ghanzi
M
M
>0<6
> 96 < 144
Lone 6
Lone 7
N/A
N/A
# 44b
# 53
Ghanzi
Ghanzi
M
M
> 30 < 48
> 96 < 144
Lone 8
Lone 9
N/A
N/A
# 54b
# C6d
# C15b
# C21b
# C33b
WD1b
# 63b
TULIb
Ghanzi
Moremi GR
Moremi GR
Moremi GR
Moremi GR
Sekoma
Tshabong
Tuli
M
M
F
F
M
M
M
F
> 30 < 48
> 30 < 48
> 48 < 96
> 30 < 48
> 48 < 96
> 48 < 96
> 48 < 96
> 30 < 48
Lone 10
Lone 11
Lone 12
Lone 13
Lone 14
Lone 15
Lone 16
Lone 17
N/A
Yes
Yes
Yes
Yes
Yes
Yes
Yes
None
None
Distantly related to # 44 (could be cousins)
None
Distantly related to # 46
Distantly related to # 53
Offspring of # 43
Father of # 40
Father of # 41 and 42
Distantly related to # 23 (could be cousins)
Distantly related to # 46
Distantly related to # 27
None
None
None
None
None
None
None
None
N/A
N/A
0.351
N/A
0.547
0.470
0.973
0.973
1.000
0.321
0.472
0.470
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
# 2b
Jwaneng
F
> 48 < 96
Female Coalition 1
Yes
#3
Jwaneng
F
> 48 < 96
Female Coalition 1
Yes
Sibling of # 3
Related to # 4 (possibly mother)
Sibling of # 2
0.853
1.000
0.853
1.000
1.000
1.000
# 58
# 58
# 58
# 58
# 58
1.000
1.000
1.000
1.000
1.000
1.000
#4
Jwaneng
F
> 30 < 48
Female Coalition 1
Yes
Related to # 4 (possibly aunt)
Related to # 2 (possibly daughter)
Related to # 3 (possibly niece)
0.853
1.000
0.853
# 14b
# 15
# 16b
Jwaneng
Jwaneng
Jwaneng
M
M
M
> 48 < 96
> 48 < 96
> 48 < 96
Male coalition 1
Male coalition 1
Male coalition 1
No
Yes
Yes
None
Sibling of # 16
Sibling of # 15
N/A
1.000
1.000
# 10b
# 11
# 24b
# 25
Ghanzi
Ghanzi
Ghanzi
Ghanzi
M
M
M
M
> 18 < 30
> 18 < 30
> 30 < 48
> 30 < 48
Male coalition 2
Male coalition 2
Male coalition 2
Male coalition 2
No
Yes
Yes
Yes
None
Sibling of # 24 and 25
Sibling of # 11 and 25
Sibling of # 11 and 24
N/A
1.000
1.000
1.000
# 45b
# 46b
Ghanzi
Ghanzi
M
M
> 30 < 48
> 30 < 48
Male coalition 3
Male coalition 3
No
No
None
Distantly related to # 53
Distantly related to # 27
N/A
0.472
0.547
# 47
Ghanzi
M
> 48 < 96
Male coalition 4
Yes
# 48
Ghanzi
M
> 48 < 96
Male coalition 4
Yes
Sibling of # 48
Related to # 32 (father or uncle)
Sibling of # 47
Related to # 32 (father or uncle)
1.000
1.000
1.000
1.000
# 12b
# 13b
Sekoma
Sekoma
M
M
> 18 < 30
> 18 < 30
Male coalition 5
Male coalition 5
No
No
None
None
N/A
N/A
# 31b
# 32b
Ghanzi
Ghanzi
F
F
> 6 < 12
> 6 < 12
Orphaned siblings 1
Orphaned siblings 1
No
No
None
Related to # 47 (daughter or niece)
Related to # 48 (daughter or niece)
N/A
1.000
1.000
# 61b
# 62
Sekhutlane
Sekhutlane
M
M
> 6 < 12
> 6 < 12
Orphaned siblings 2
Orphaned siblings 2
Yes
Yes
Sibling of # 62
Sibling of # 61
1.000
1.000
a
As determined by grouping and behaviour at time of capture;
b
Individuals used in population genetic analysis;
c
Genetic
probability as determined by COLONY. M: Male; F: Female; d Individual #C6 was not included in the analysis as its profile was
only 56% completed.
Table S2: Total number of alleles observed and allele size ranges in unrelated
Botswana cheetahs for each microsatellite locus (n = 32).
a
Microsatellite locia
Number of allelesb
Allele size range in bpb
FCA005
5 (6)
139-149 (137-149)
FCA008
6 (6)
149-163
FCA014
6 (8)
152-174 (152-162)
FCA026
5 (6)
148-156 (146-156)
FCA069
6 (6)
96-108
FCA085
8 (9)
118-142
FCA096
8 (8)
199-213
FCA097
5 (5)
139-151
FCA105
5 (5)
183-197
FCA126
4 (4)
118-128
FCA171
8 (8)
95-111
FCA212
4 (5)
120-126
FCA214
6 (7)
166-180
FCA310
6 (7)
121-131 (117-131)
Nuclear markers described in Menotti-Raymond et al. 1999. None of the markers
deviate from Hardy-Weinberg equilibrium.
b
The values indicated in brackets were observed for all samples (including relatives;
n = 73).
Table S3: Genetic distances FST (below diagonal) and RST (above diagonal).
FST / RST
Moremi
(n=4)
Ghanzi
(n=14)
Jwaneng Sekoma
(n=8)
(n=3)
Moremi
-
0.079*
0.000
0.052
Ghanzi
0.085*
-
0.068*
0.119*
Jwaneng
0.083*
0.035*
-
0.000
Sekoma
0.033
0.000
0.000
-
* Significant values (p-value < 0.05). Populations from Sekhutlane, Tshabong and
Tuli were represented by only one individual each and therefore were not included
into the analysis.
Figure S1: Factorial Correspondence Analysis (FCA) of 32 unrelated individuals
based on 14 microsatellite loci.
M: Moremi Game Reserve (n = 4); G: Ghanzi (n = 14); J: Jwaneng (n = 8); S: Sekoma
(n = 3); SKL: Sekhutlane (n = 1); T: Tshabong (n = 1); Tuli: Tuli (n = 1).