Use of GEM Germplasm for Evaluation and Development of Drought Tolerance, Corn Ear
Worm (CEW) Resistance, and Low Aflatoxin Level Grain
Wenwei Xu*, Thomas Marek, Gary Odvody, and Paul Williams
*Texas A&M Agric. Res. and Ext. Center, Lubbock, Texas, E-mail: we-xu@tamu.edu
Objectives:.
This report serves to document research conducted under a specific cooperative
agreement between ARS and Texas A&M University. It covers progress from October 1, 2009
to September 30, 2010. Specific objectives of this project include: (1) Select, advance, and
release stress-tolerant inbred lines from GEM germplasm; (2) Evaluate GEM breeding crosses
for drought and heat tolerance, corn ear worm resistance, and agronomic performance in Texas.
Testcrosses of the lines from AR03056:N0902, BR52051:N04, CUBA117:S15, DK888:N11, and
SCROGP3:N1411a have been widely tested. New inbred lines ANTIGO01:N16, DK888:Na08f,
DKXL380:S08a and GUAT209:N19 have been developed. This reports highlights the results of
the new lines.
1. Testcrosses of GEM Lines: About 100 testcrosses between GEM lines (developed in
Lubbock) with public and licensed proprietary lines were evalauted for grain yield, CEW
resistance, and drought tolerance in the Texas High Plains (Lubbock, Halfway, and Dumas)
under well-watered and drought conditions and in the south-central Texas (College Station,
Corpus Christi, Ganado, Uvalde, and Wharton). Eight GEM testcrosses were evaluated for
silage yield. Four GEM hybrids were entered in the Texas state silage performance tests. New
test crosses are made for 2011 research.
Tables 1 to 3 shows the yield, days to pollen shedding and stay green rating foor the
testcroosses of the lines developed from DK888:Na08f and DKXL380:S08a. These testcrosses
had lower grain yield, similar maturity, and better stay green rating as compared to the
commercial checks.
Inbred lines from ANTIG01:N16 have nice looking plants and excellent grain quality
with low grain molds, however, these lines have a high ear placement. Their testcrosses shed
pollens close to the hybrids with a relative muarity of 117 day and yield slightly lower than
commercial checks (Table 4).
2. Inbred line development and characterization: Inbred lines developed from the breeding.
crosses ANTIGO01:N16AR03056:N0902, BR52051:N04, CH05012:N12, CUBA164:S20,
CUBA117:S15, DK888:N11, FS8A(T):N11a, FS8A(T):N1801, SCROGP3:N2017, and
SCROPG3:N1411a have been characterized as line per se and in the testcross combinations for
agronomic traits and stress tolerance under well watered and drought conditions in Lubbock and
Halfway for a second year.
3. Aflatoxin analysis: Aflatoxin resistance of the GEM-line hybids were tested for a second
year in 2010. This 34-entry test included five hybrids made with Tx205 and three hybrids made
with the DKXL370:N11a20 lines. The test were conducted in multiple locations in Texas and
Mississippi. Three hybrids of DKXL370:N11a20 and two hybrids of Tx205 had low aflatoxin in
2009. In addition, three hybrids containing one parent lined derived from DK888:N11,
1
CUBA117:S15, and BR5251:N04 were included in the SERAT tests. SERAT is a muti-state
afaltoxin testing network. Garin samples from 2010 tests are being analyzed for aflatoxin.
4. Biomass and silage quality: Nine test crosses of GEM-lines were included in a 36 entry
silage yield trial at Dumas and Halfway, Texas. The GEM-lines are the inbred lines developed
by our project from AR01150:N04, BR52051:N04, CUBA117:S15, DKXL370:N11a20, and
GUAT209:N19. The testcrosses were chosen for their height and good grain yield in previous
trials. The average biomass yield at both locations was 31.7 and 31.6 tons per acre (adjusted to
65% moisture) for the testcrosses of the inbred lines developed from BR52051:N04 and
GUAT209:N19, respetively. The biomass yield was comparable to the best commercial silage
hybrids (Table 5). These GEM-lines may be a good source for biofuel and silage corn
production.
Five testcrosses of GEM lines were included in the 2010 Texas State Silage Corn
Performance Test in the High Plains. The test crosses are listed under the name AgriLife (XU)
which is the same as Texas A&M University. These five testcrooses have a line WXY10A is
the testcross of a line from BR52051:N04; WXY10B is the testcross of a line from
SCROGP3:N2017; WXC10C is the testcross of a line from CUBA117:S15; WXY10D is the
testcross of Tx205, a GEM line released in 2004; and WXY10F is the testcross of a line
developed from a breeding cross involving GUAT209:N19. Yield, agronomic data, and silage
quality of these testcrosses and other commercial hybrids are shown in Tables 6-8.
Germplasm distribution: We have distributed seeds of Tx204, Tx205, and four inbred lines (to
be released) to 14 seed companies. Breeders, agronomists, and sales reps toured yield trial plots
involving GEM top crosses with seed company lines during the growing season.
Communications on GEM project:
The GEM project and use of the GEM germplasm in our breeding program were specifically
mentioned in the following publications and presentation.
Presentations and publication covering GEM project and germplasm:
Wenwei Xu, Gary Odvody, Paul Willaims, and Mike Blanco. Use of tropcal germplasm to
improve stress tolerance and aflatoxin acculumlation in corn. NCC167 North Central Corn
Breeding Committee Meeting in Chicago on April 6-7, 2010.
Wenwei Xu, Thomas Marek, Andy Cranmer, Bruce Carlson, Jonny Beck, Brent Bean, and
Dennis Pietsch. 2010. 2010 State Silage Corn Performance Test on the Texas High Plains.
Texas AgriLife Research and Extension-Lubbock Center Technical Report No.10-3. pp.12.
Wenwei Xu, Junping Chen, and Zhanguo Xin. Exploring native genetic variation for improving
drought tolerance and water use efficiency in corn. ASA-CSSA-SSSA annual meeting in
Long Beach, CA, October 31 – November 4.
Wenwe Xu. Developing drought and heat tolerant crops to mitigate the effects of climate
change. An invited plenary talk at the Symposium 'Plants meeting future challenges - food
and energy for the society in a changing environment”. University of Zurich, Switzerland.
2
September 10, 2010.
Wenwei Xu. Genetic resources for corn breeding, part of the Moelcular Corn Breeding Training
Course (invited). National Maize Research Institute, Hanois, Vietnam, May 10-14, 2010.
Wenwei Xu. Breeding drought tolerant corn (invited talk). Maize Research Institute, Agricultural
Academy of Jilin Province, Changchun, China, November 11, 2010.
Wenwei Xu. Genetic improvement of maize water use efficiency (invited talk). Crop Research
Institute, Chinesew Academy of Agricultural Sciences, Beijing, China, November 12, 2010.
Field days and presentations:
Corn breeding field day. College Station, Texas, June 29, 2010.
Texas High Plains limited irrigation corn field day. Etter, Texas, August 25, 2010.
These field days were held for corn producers, seed companies, policy makers, consultants,
agriculture professionals, and underground water conservation districts. GEM lines and hybrids
were grown in the field plots and discussed.
3
Table 1. Grain yield (bu/a, adjusted to 15.5% moisture) of testcrosses of the lines develpped from
DK888:N11a08f (entries 1 to 15) and DKXL370:N11a20 (entries 16 to 26) under well-watered
(WW) and drought (DRT) conditions in Halfway (HF), Dumas (DS), and Lubbock (LB), Texas
in 2009 and 2010.
ENO
Pedigree
HF-WW
2010
DS-WW
2010
DS-WW
2009
LB
2009
HF-DT
2009
Mean
1
2
3
4
5
DK8F1 x B110
DK8F1 x SS1
DK8F2 x B110
DK8F3 x B110
DK8F3 x SS2
185.3
163.7
173.2
193.9
204.1
182.7
139.7
142.9
219.5
225.5
218.1
172.8
184.8
218.1
207.5
117.8
114.6
91.5
120.5
116.2
40.7
47.7
66.1
75.0
89.1
148.9
127.7
131.7
165.4
168.5
6
7
8
9
10
DK8F3 x SS1
DK8F4 x SS3
DK8F6 x SS3
DK8F6 x SS1
DK8F6 x B110
199.6
202.3
193.3
196.4
184.4
208.0
199.7
202.3
208.0
217.9
213.5
232.1
239.7
235.4
252.5
124.5
122.0
142.9
133.2
109.4
78.9
72.5
85.3
66.1
64.2
164.9
165.7
172.7
167.8
165.7
11
12
13
14
15
DK8F8 x SS3
DK8F10 x SS3
DK8F10 x SS1
DK8F10 x B110
DK8F12 x SS3
186.7
205.7
207.8
188.4
179.8
185.3
227.5
186.5
156.4
171.3
190.9
230.0
216.6
206.8
218.2
125.3
126.0
116.8
124.6
124.9
78.9
64.8
84.5
80.2
80.0
153.4
170.8
162.4
151.3
154.8
16
17
18
19
20
DKXL1 x SS3
DKXL1x B110
DKXL2 x SS3
DKXL2 x CUBA1
DKXL3 x SS3
217.2
165.9
179.4
.
197.6
190.9
138.9
210.5
246.6
173.2
216.6
204.3
235.7
215.9
271.4
118.7
79.0
81.2
137.1
121.5
63.9
38.0
66.0
100.4
64.6
161.5
125.2
154.6
175.0
165.7
21
22
23
24
25
26
DKXL4 x SS3
DKXL5 x B110
DKXL6 x B110
DKXL8 x B110
DKXL8 x SS2
DKXL10 x B110
215.4
191.9
209.1
205.6
160.6
166.3
219.1
200.1
179.1
216.1
168.3
213.0
242.4
227.6
209.3
172.2
212.3
207.8
109.1
118.9
100.3
90.1
95.3
92.2
35.3
53.2
60.7
35.7
74.3
66.1
164.3
158.3
151.7
143.9
142.2
149.1
27
28
29
30
DKC66-23
DKC67-87
82H80GT/CB/LL
Pioneer 34F96
221.5
250.2
241.7
227.6
221.8
254.9
221.2
211.7
286.1
285.7
263.3
300.2
137.6
132.2
132.2
120.4
89.8
86.6
90.3
85.1
191.3
201.9
189.7
189.0
Test mean
CK mean
CV%
LSD 0.05
197.1
235.2
8.9
36.0
197.9
227.4
9.8
39.7
226.3
283.8
8.5
39.2
115.9
130.6
14.7
34.9
69.5
87.9
22.6
32.2
161.2
193.0
SS1, SS2, and SS3 are two propriatary stiff stalk testers.
4
Table 2. Days to pollen shed for testcrosses of the lines develpped from DK888:N11a08f (entries
1 to 15) and DKXL370:N11a20 (entries 16 to 26) under well-watered (WW) and drought (DRT)
conditions in Halfway (HF), Dumas (DS), and Lubbock (LB), Texas in 2009 and 2010.
ENO
Pedigree
HF-WW
DS-WW
DS-WW
HF-DRT
LB-WW
2010
2010
2009
2009
2009
Mean
1
DK8F1 x B110
69.5
70.0
74.0
74.0
73.0
72.1
2
DK8F1 x SS1
68.5
70.0
73.0
75.0
76.0
72.5
3
DK8F2 x B110
69.5
70.0
75.0
73.0
74.0
72.3
4
DK8F3 x B110
68.5
69.0
73.0
72.0
74.0
71.3
5
DK8F3 x SS2
68.5
69.0
74.0
73.0
73.0
71.5
6
DK8F3 x SS1
69.0
71.0
74.0
74.0
75.0
72.6
7
DK8F4 x SS3
69.0
71.0
76.0
75.0
76.0
73.4
8
DK8F6 x SS3
70.0
70.0
76.0
75.0
76.0
73.4
9
DK8F6 x SS1
69.0
70.0
75.0
74.0
76.0
72.8
10
DK8F6 x B110
69.0
69.0
73.0
74.0
73.0
71.6
11
DK8F8 x SS3
69.5
71.0
75.0
74.0
76.0
73.1
12
DK8F10 x SS3
69.5
71.0
77.0
75.0
76.0
73.7
13
DK8F10 x SS1
69.5
69.0
75.0
74.0
74.0
72.3
14
DK8F10 x B110
68.5
71.0
73.0
73.0
73.0
71.7
15
DK8F12 x SS3
69.5
71.0
75.0
74.0
75.0
72.9
16
DKXL1 x SS3
68.0
69.0
74.0
75.0
75.0
72.2
17
DKXL1x B110
68.0
69.0
72.0
75.0
74.0
71.6
18
DKXL2 x SS3
67.0
69.0
74.0
74.0
76.0
72.0
19
DKXK2 x CUBA1
.
70.0
73.0
72.0
73.0
72.0
20
DKXL3 x SS3
68.0
69.0
75.0
75.0
75.0
72.4
21
DKXL4 x SS3
69.5
71.0
75.0
74.0
76.0
73.1
22
DKXL5 x B110
69.0
69.0
73.0
72.0
74.0
71.4
23
DKXL6 x B110
69.0
70.0
73.0
74.0
74.0
72.0
24
DKXL8 x B110
66.0
67.5
75.0
76.0
75.0
71.9
25
DKXL8 x SS2
65.0
69.0
73.0
71.0
73.0
70.2
26
DKXL10 x B110
66.5
69.0
72.0
70.0
73.0
70.1
27
DKC66-23
67.0
67.5
72.0
70.0
72.0
69.7
28
DKC67-87
66.0
70.0
73.0
73.0
73.0
71.0
29
82H80GT/CB/LL
66.0
69.0
72.0
72.0
72.0
70.2
30
Pioneer 34F96
66.0
69.0
73.0
69.0
72.0
69.8
Test mean
68.2
69.6
73.9
73.4
74.2
71.9
CK mean
66.3
68.9
72.5
71.0
72.3
70.2
CV%
1.3
1.3
1.6
1.0
0.9
LSD 0.05
1.7
1.9
2.5
1.5
1.3
5
Table 3. Stay green ratings for testcrosses of the lines develpped from DK888:N11a08f (entries 1
to 15) and DKXL370:N11a20 (entries 16 to 26) under well-watered (WW) and drought (DRT)
conditions in Halfway (HF), Dumas (DS), and Lubbock (LB), Texas in 2009 and 2010.
ENO
Pedigree
1
DK8F1 x B110
2
DK8F1 x SS1
3
DK8F2 x B110
4
DK8F3 x B110
5
DK8F3 x SS2
6
DK8F3 x SS1
7
DK8F4 x SS3
8
DK8F6 x SS3
9
DK8F6 x SS1
10
DK8F6 x B110
11
DK8F8 x SS3
12
DK8F10 x SS3
13
DK8F10 x SS1
14
DK8F10 x B110
15
DK8F12 x SS3
16
DKXL1 x SS3
17
DKXL1x B110
18
DKXL2 x SS3
19
DKXK2 x CUBA1
20
DKXL3 x SS3
21
DKXL4 x SS3
22
DKXL5 x B110
23
DKXL6 x B110
24
DKXL8 x B110
25
DKXL8 x SS2
26
DKXL10 x B110
27
DKC66-23
28
DKC67-87
29
82H80GT/CB/LL
30
Pioneer 34F96
Test mean
CK mean
CV%
LSD 0.05
HF-WW
2010
1.4
1.8
1.8
2.0
2.0
1.8
1.5
1.4
1.8
2.0
1.5
1.8
1.8
2.0
1.5
1.5
1.8
1.5
.
1.4
1.2
1.4
1.5
2.3
2.7
2.0
2.0
1.4
1.4
1.8
DS-WW
2010
2.8
3.3
2.8
3.4
2.5
2.7
2.9
2.9
3.3
3.3
2.9
3.0
3.3
2.9
3.0
2.5
2.9
2.3
2.5
3.4
2.0
2.3
3.2
2.5
3.5
2.9
2.9
1.8
2.5
2.4
HF-DRT
2009
2.9
2.9
2.8
3.0
3.0
2.7
3.0
2.9
3.0
2.9
3.0
3.2
2.9
3.0
3.0
2.7
3.0
3.3
3.0
2.8
3.2
2.3
2.8
2.9
2.7
3.0
4.3
3.0
3.8
3.3
HF-DRT
2009
3.3
3.5
3.3
3.8
4.0
3.5
3.8
4.0
4.0
3.8
4.0
3.8
4.0
4.0
3.5
3.5
3.5
4.0
3.8
3.0
3.3
3.3
3.9
3.5
3.8
4.0
4.5
3.8
4.8
4.3
LB-WW
2009
3.0
2.3
2.7
3.0
2.3
2.3
2.5
3.0
2.5
3.0
2.5
2.3
2.5
3.0
2.5
2.0
3.0
3.0
3.5
2.0
2.0
2.5
3.3
3.3
3.8
3.3
3.3
2.3
3.5
2.5
Mean
1.7
1.6
15.4
0.5
2.8
2.4
16.4
ns
3.0
3.6
8.9
0.5
3.8
4.3
9.4
0.7
2.7
2.9
10.1
0.6
2.8
3.0
6
2.7
2.7
2.6
3.0
2.8
2.6
2.7
2.8
2.9
3.0
2.8
2.8
2.9
3.0
2.7
2.4
2.8
2.8
3.2
2.5
2.3
2.3
2.9
2.9
3.3
3.0
3.4
2.4
3.2
2.8
Table 4. Days to pollen shed, plant and ear height, and grain yield of testcrosses of the lines
develpped from ANTIG01:N16 in Dumas (DS) and Halfway (HF), Texas in 2010.
Pedigree
ANTIGO1/2 x SS3
ANTIGO3 x SS3
ANTIGO4 x SS3
ANTIGO5 x SS3
ANTIGO6 x SS3
Days to pollen
Plant ht., cm
Ear ht., cm
Grain yield (bu/a)
DS
HF
DS
HF
DS
HF
DS
HF
Mean
72
71
290
308
125
138 163.8
205.9 184.9
72
69
304
301
136
150 186.2
219.3 202.8
73
69
305
306
122
138 192.4
214.5 203.5
73
70
303
303
137
144 226.1
207.2 216.6
73
70
305
306
141
150 198.1
206.6 202.3
ANTIGO7 x SS3
ANTIGO8 x SS3
ANTIGO9 x SS3
ANTIGO11/12 x SS3
ANTIGO13 x SS3
71
71
72
71
71
69
71
67
68
70
293
299
288
297
292
299
283
253
282
279
142
132
120
122
127
151
157
93
129
92
157.7
185.6
179.0
182.0
155.3
215.9
237.9
228.6
234.6
227.4
186.8
211.8
203.8
208.3
191.4
ANTIGO14 x SS3
ANTIGO15/16 x SS3
ANTIGO17/18 x SS3
ANTIGO19/20 x SS3
71
71
73
73
70
70
70
71
288
295
257
293
299
292
301
294
131
135
131
118
164
135
144
128
171.2
187.2
179.3
186.6
227.4
222.6
225.1
212.6
199.3
204.9
202.2
199.6
DKC66-23 (RM116)
DKC67-87 (RM117)
Pioneer 34F96 (RM110)
82H80GT/CB/LL
66
70
69
69
65
67
64
68
266
286
260
291
288
289
272
310
95
108
88
93
114
134
87
152
250.6
245.8
229.4
249.0
249.0
249.6
215.3
243.6
249.8
247.7
222.3
246.3
Testcross mean
Test mean
CK Mean
CV%
LSD 0.05
72
71
69
1
1
69
69
66
2
4
293
289
276
4
26
293
292
289
4
25
130
122
96
10
27
136
133
122
8
23
182.2
195.9
243.7
11.1
49.5
220.4
224.6
239.4
5.1
24.4
201.3
210.2
241.5
SS3 is a proprietary stiff stalk tester line.
7
Table 5. Days to pollens shed (DTP), plant and ear height, and total biomass of GEM-line
testcrosses at Dumas (DS) and Halfway (HF), Texas in 2010. The test had 36 entries. The table
only shows the yield of the GEM-line-testcrosses and three commercial check hybrids. Yield
was adjusted to 65% moisture. Pedigrees of the lines are abbreviated due to space limitation.
ENO
Pedigrees
DS
19
AR1150N04-D x SS3
20
AR1150N04-F x SS3
21
AR1150N04-G x SS3
22
AR1150N04-G x CUBA1
23
BR1 x SS1
24
DKXL1 x SS3
25
DKXL2 x SS3
26
DKXL3 x SS3
28
C3W64-4 x CUBA1
30
GUAT10/13 x SS3
34
TMF2N804
35
DKC67-87
36
P31G96
Test Mean
CK mean
CV%
LSD 0.05
Plant ht.
cm
DTP
HF
Ear ht.
cm
Forage yield
tons/a
Yield rank
DS
HF
DS
HF
DS
HF
Mean
DS
HF
69
69
69
69
71
68
69
68
69
73
75
69
71
77
77
79
78
78
78
77
77
79
80
80
76
78
286
295
303
273
293
279
275
292
288
306
305
287
297
286
296
294
283
293
270
284
281
288
297
300
285
309
116
130
115
110
116
97
112
80
117
141
96
115
126
114
115
112
128
122
115
115
100
121
125
114
122
120
21.2
24.8
25.7
24.8
31.9
28.0
28.5
27.2
31.5
32.3
30.8
32.1
34.0
31.3
26.9
30.5
28.6
31.4
26.5
27.3
24.9
26.6
30.8
30.0
31.9
31.5
26.2
25.8
28.1
26.7
31.7
27.3
27.9
26.1
29.1
31.6
30.4
32.0
32.8
34
20
15
18
5
9
8
11
6
3
7
4
2
4
30
6
21
3
34
25
36
33
5
8
1
2
70
72
2
3
78
78
1
21
291 286
296 298
3
4
16 21
113 114
112 119
14
8
32 19
25.8
32.3
8.2
4.9
28.7
31.1
6.2
3.6
27.2
31.7
Note: AR1150N4-D, AR1150N04-F, and AR1150N04-G are the lines developed from GEM
breeding cross AR01150:N04; BR1 is a line developed from BR52051:N04; CUBA1 is a line
developed from CUBA117:S15; DKXL1, DKXL2, and DKXL3 are lines developed from
DKXL370:N11a20; and GUAT10/13 are two sister lines developed from GUAT209:N19. SS1
and SS3 are two propriatary stiff stalk testers.
8
Table 6. Means of forage yield adjusted to 65% moisture and agronomic traits of the State Silage Corn Performance Test at Halfway,
Texas in 2010.
ENO
Hybrid
Company
BH 8895VTTP
BH9018VTTP
X9150G
X9151G
70R50
B-H Genetics
B-H Genetics
B-H Genetics
B-H Genetics
Blue River Hybrids
118-120
118-120
116-118
115-117
114
6
7
8
9
10
73B33
58VP30
56VP69
CX10617
CX10115
Blue River Hybrids
CPS DynaGro
CPS DynaGro
CPS DynaGro
CPS DynaGro
11
12
13
14
15
DKC67-88
FZF622
TMF2W727
TMF2N804
TMF2L844
16
17
18
19
20
1825V
8539R
2288H
Integra EXP
952115GT
Integra 9701VT3
21
22
23
24
25
Integra
9676VTPRO
Integra 9651VT3
WXY10A
WXY10B
WXY10C
1
2
3
4
5
RM
Stand
%
Traits
Days to
pollen
shed
Plant
ht, in.
Ear
ht, in
Moist.,
%
Yield,
tons/a
Yield
rank
VT Triple Pro
VT Triple Pro
GT
GT
Non-GMO
96.6
93.9
90.9
96.2
98.1
69.0
67.0
67.0
67.0
66.0
109.1
112.5
112.7
117.7
112.9
41.9
49.9
45.5
50.7
44.2
65.8
63.8
64.3
65.7
61.5
31.09
30.27
28.98
30.74
27.61
9
16
21
12
27
114
118
116
117
115
Non-GMO
VT Pro
VT Pro
VT3
GT
96.2
96.6
86.0
94.3
91.7
67.0
66.0
67.0
66.0
66.0
116.3
112.5
108.1
109.4
97.8
46.1
47.4
47.9
39.4
33.6
66.4
62.5
61.8
64.7
63.9
29.09
28.70
29.21
28.51
26.90
20
23
19
24
28
Monsanto
Mycogen
Mycogen
Mycogen
Mycogen
117
109
113
116
118
GENVT3P
RR2, bm3
None
None
RR2
98.1
97.0
98.9
96.6
95.5
68.0
67.0
68.0
70.0
69.0
115.7
114.8
117.7
124.3
125.3
50.4
45.1
47.4
39.6
50.5
63.7
60.3
66.6
68.3
67.6
32.58
29.99
30.45
30.83
33.35
4
17
14
11
2
Triumph Seed
Triumph Seed
Triumph Seed
118
118
122
VT3
RR
HX1/RR
95.5
88.3
93.9
69.0
70.0
70.0
121.3
125.2
122.4
45.7
45.8
52.2
64.4
68.9
66.8
31.66
32.32
31.34
7
5
8
Wilbur-Ellis
Wilbur-Ellis
115
120
GT
VT3
93.2
91.7
68.0
68.0
120.9
116.4
51.2
49.5
67.2
62.8
32.12
33.42
6
1
Wilbur-Ellis
Wilbur-Ellis
AgriLife (XU)*
AgriLife (XU)
AgriLife (XU)
117
115
118
118
117
VT3PRO
VT3
None
None
None
98.9
98.1
98.9
96.2
97.0
66.0
67.0
69.0
69.0
68.0
111.4
99.7
113.9
117.3
111.0
46.1
33.9
52.6
50.8
45.8
63.1
61.4
62.5
62.8
62.6
28.93
30.90
30.56
32.91
29.52
22
10
13
3
18
9
ENO
26
27
28
Hybrid
WXY10D
WXY10E
WXY10F
Company
AgriLife (XU)
AgriLife (XU)
AgriLife (XU)
RM
118
117
119
Traits
None
None
None
Test mean
CV%
LSD 0.05
Stand
%
97.0
94.3
90.9
Days to
pollen
shed
69.0
68.0
70.0
Plant
ht, in.
110.5
113.6
118.0
Ear
ht, in
44.1
43.4
47.1
Moist.,
%
69.5
66.8
65.8
Yield,
tons/a
27.92
30.38
28.26
95.0
5.0
ns
67.9
1.4
1.6
114.6
3.2
6.1
46.0
7.9
6.0
64.7
2.6
2.7
30.30
7.63
3.79
Yield
rank
26
15
25
Note*: AgriLife is same as Texas A&M University. WXY10A is the testcross of a line from BR52051:N04; WXY10B is the testcross
of a line from SCROGP3:N2017; WXC10C is the testcross of a line from CUBA117:S15; WXY10D is the testcross of Tx205, a GEM
line released in 2004; and WXY10F is the testcross of a line developed from a breeding cross involving GUAT209:N19.
10
Table 7. Forage quality of the State Silage Corn Performance Test at Halfway, Texas in 2010.
ENO Hybrid
Company
CP ADF NDF Lignin NFC Starch TDN
BH 8895VTTP
BH9018VTTP
X9150G
X9151G
70R50
B-H Genetics
B-H Genetics
B-H Genetics
B-H Genetics
Blue River Hybrids
6
7
8
9
10
73B33
58VP30
56VP69
CX10617
CX10115
Blue River Hybrids
CPS DynaGro
CPS DynaGro
CPS DynaGro
CPS DynaGro
11
12
13
14
15
DKC67-88
FZF622
TMF2W727
TMF2N804
TMF2L844
Monsanto
Mycogen
Mycogen
Mycogen
Mycogen
16
17
18
Triumph Seed
Triumph Seed
Triumph Seed
19.7
17.3
19.2
22.6
17.6
19.5
19.2
20.6
19.7
17.3
22.3
26.1
22.6
22.8
29.7
18.8
24.4
26.7
20.0
24.8
21.2
34.4
30.6
34.2
39.0
31.6
33.9
33.1
35.9
34.8
30.8
38.6
44.2
38.1
38.7
48.6
33.3
41.2
45.3
34.5
41.9
36.4
3.2
3.2
3.5
3.5
3.1
3.4
3.4
3.8
3.4
3.2
3.7
3.9
3.9
3.8
4.3
3.4
3.6
4.0
3.3
3.5
3.4
52.1
54.6
51.7
47.7
53.9
51.6
52.5
49.4
50.5
54.7
47.4
42.7
47.5
46.7
37.8
52.4
44.4
41.1
51.5
44.3
50.0
45.0
47.0
42.6
40.0
46.6
42.8
45.4
41.0
42.7
47.0
38.5
36.4
38.8
38.5
28.7
44.3
36.9
32.0
44.5
36.7
41.8
19
20
1825V
8539R
2288H
Integra EXP
952115GT
Integra 9701VT3
8.3
9.3
9.4
8.4
9.1
9.3
9.1
9.4
9.4
9.1
9.1
8.5
9.0
9.0
7.9
9.2
8.8
8.6
8.4
8.3
8.7
Wilbur-Ellis
Wilbur-Ellis
9.2
8.6
9.5
17.1
20.2
16.8
29.7
36.0
29.9
3.1
3.3
3.1
56.1
50.8
55.5
21
22
23
24
25
Integra
9676VTPRO
Integra 9651VT3
WXY10A
WXY10B
WXY10C
Wilbur-Ellis
Wilbur-Ellis
AgriLife (XU)
AgriLife (XU)
AgriLife (XU)
9.3
8.3
9.3
9.4
8.4
23.7
19.7
17.3
19.2
22.6
41.0
34.4
30.6
34.2
39.0
3.9
3.2
3.2
3.5
3.5
44.6
52.1
54.6
51.7
47.7
1
2
3
4
5
11
IVTD24
NDFD24
MILK1 MILK2
Ash
77.0
77.0
76.0
73.0
79.0
75.0
77.0
74.0
77.0
77.0
72.0
74.0
74.0
74.0
68.0
77.0
72.0
71.0
75.0
74.0
73.0
81.0
82.3
80.0
76.3
82.7
79.7
81.0
78.0
80.3
82.0
76.3
79.3
78.0
77.3
70.7
80.7
75.3
73.0
78.7
76.7
77.7
43.7
41.7
42.0
39.0
45.0
40.0
43.0
39.3
43.7
41.7
38.3
53.3
42.3
42.0
39.7
42.3
40.7
39.7
39.3
44.0
39.0
3065.3
3052.7
3025.0
2885.7
3193.7
3001.0
3058.7
2966.3
3087.0
3079.0
2886.3
3102.3
2977.3
2952.3
2697.7
3068.7
2858.3
2853.3
2909.0
2965.3
2875.7
3430.3
3433.0
3370.0
3209.3
3571.0
3348.0
3426.3
3298.7
3433.3
3459.3
3198.3
3397.0
3291.7
3264.0
2930.0
3427.7
3158.0
3113.3
3269.7
3263.0
3215.0
4.02
4.20
3.80
3.70
3.72
3.90
4.00
3.89
4.04
3.95
3.89
4.08
4.44
4.52
4.97
3.94
4.59
4.02
4.32
4.54
4.00
48.5
43.1
47.8
78.0
76.0
79.0
83.0
80.0
82.7
42.7
43.3
41.7
3110.0
3066.0
3160.0
3502.7
3416.0
3548.0
3.75
3.43
3.42
33.5
45.0
47.0
42.6
40.0
74.0
77.0
77.0
76.0
73.0
76.3
81.0
82.3
80.0
76.3
42.0
43.7
41.7
42.0
39.0
3053.3
3065.3
3052.7
3025.0
2885.7
3325.0
3430.3
3433.0
3370.0
3209.3
4.03
4.02
4.20
3.80
3.70
ENO Hybrid
26
27
28
WXY10D
WXY10E
WXY10F
Test mean
CV%
LSD 0.05
Company
CP ADF NDF Lignin NFC Starch TDN
IVTD24
NDFD24
AgriLife (XU)
AgriLife (XU)
AgriLife (XU)
9.6
9.8
9.2
23.1
19.5
22.6
38.5
32.9
38.3
4.0
3.7
3.9
46.1
51.7
47.0
35.7
42.4
37.6
74.0
76.0
74.0
77.3
81.0
77.0
41.7
41.7
39.7
MILK1 MILK2
2977.3
3040.0
2989.7
3266.0
3383.0
3294.3
Ash
4.87
4.58
4.09
9.0
5.4
0.8
21.3
14.7
5.1
36.6
13.3
ns
3.6
10.9
0.6
49.2
9.0
7.2
40.9
12.0
8.1
74.9
3.6
4.4
78.7
3.9
5.0
41.9
6.1
4.2
2998.5
3.7
179.3
3330.1
4.2
228.6
4.10
10.80
0.73
1. IVTD24: In vitro true digestibility (IVTD) after 24 hours of incubation in rumen fluid. It measures digestibility and can be used to estimate
energy. A higher value of IVTD 24 hr presents a better forage quality.
2. Forage nutritional values based on NIR analysis.
ADF: Acid detergent fiber, a measure of cellulose and lignin. ADF is negatively correlated with overall digestibility.
CP: Crude protein, the total protein in the sample including true protein and non-protein nitrogen.
Lignin: undigestible plant component and has a negative impact on cellulose digestibility.
NDF: Neutral detergent fiber, a measure of hemicellulose, cellulose and lignin representing the fibrous bulk of the forage. NDF is negatively
correlated with intake
NFC: Percentage of non-fibrous carbohydrates; estimates the amount of rapidly digestible carbohydrates in a forage.
NDFD24: Percentage of NDF that is digestible by in vitro incubation.
MILK 1: Estimated lbs. of milk produced per ton of dry matter.
MILK 2: Estimated lbs. of milk produced per ton of processed dry matter.
Starch: primarily in the grain, later maturing hybrids have lower starch since all hybrids were harvested at the same time.
TDN: Total digestible nutrients. It represents the sum of the digestible protein, digestible nitrogen-free extract, digestible crude fiber and 2.25X
the digestible fat.
Milk lbs./ton of DM: an estimated potential milk yield per ton of forage dry matter based on digestibility and energy content of the forage.
12
Table 8. Means of forage yield adjusted to 65% moisture and agronomic traits of the State Silage Corn Performance Test at Etter,
Texas in 2010.
ENO Hybrid
1
2
3
4
Company
BH 8895VTTP
BH9018VTTP
X9150G
X9151G
B-H Genetics
B-H Genetics
B-H Genetics
B-H Genetics
5
6
7
8
9
10
70R50
73B33
71K36
74A60
58VP30
56VP69
Blue River Hybrids
Blue River Hybrids
Blue River Hybrids
Blue River Hybrids
CPS DynaGro
CPS DynaGro
11
12
CX10617
CX10115
13
GA 28V71
14
GA X6515
15
RM
Plant
ht, in.
Ear
ht, in
Moist%
Yield
tons/a
Yield
rank
VT Triple Pro
VT Triple Pro
GT
GT
69.0
70.0
69.0
70.0
109.8
112.3
109.7
118.2
41.3
42.8
37.4
49.0
59.2
55.7
58.5
56.5
32.34
33.62
28.80
33.18
12
5
31
7
114
114
114
115
118
116
None GMO
None GMO
None GMO
None GMO
VT Pro
VT Pro
66.0
71.0
69.0
66.0
69.0
69.0
113.4
113.9
113.8
108.3
114.7
109.6
45.8
45.1
48.6
44.5
50.0
42.8
53.9
60.9
59.2
58.0
56.1
57.4
27.01
30.46
32.56
29.85
32.64
32.12
32
23
10
26
9
14
117
115
VT3
GT
69.0
66.0
112.2
104.9
41.9
40.8
58.8
59.8
29.57
31.73
28
17
118
VT3 Pro
69.0
116.8
46.1
59.3
33.79
4
119
VT3
70.0
120.1
52.4
60.2
34.38
2
GA X6022
CPS DynaGro
CPS DynaGro
Golden Acres
Genetics
Golden Acres
Genetics
Golden Acres
Genetics
117
GTCBLL
69.0
114.3
47.9
55.9
30.79
20
16
17
18
19
20
DKC67-88
FZF622
TMF2W727
TMF2N804
TMF2L844
Monsanto
Mycogen
Mycogen
Mycogen
Mycogen
117
109
113
116
118
GENVT3P
RR2, bm3
None
None
RR2
71.0
69.0
69.0
73.0
73.0
113.4
115.1
117.8
123.0
120.7
49.1
45.9
43.2
39.1
47.9
59.0
57.8
60.0
60.0
58.7
31.61
29.18
31.74
30.40
32.15
18
29
16
24
13
21
22
23
24
25
1825V
8539R
2288H
Integra EXP 952115GT
Integra 9701VT3
Triumph Seed
Triumph Seed
Triumph Seed
Wilbur-Ellis
Wilbur-Ellis
118
118
122
115
120
VT3
RR
HX1/RR
GT
VT3
71.0
72.0
73.0
71.0
72.0
115.5
127.0
123.8
117.7
113.3
44.4
52.6
59.8
42.4
46.3
57.8
58.4
63.3
58.4
56.1
34.94
30.57
31.57
33.25
33.88
1
21
19
6
3
Company
RM
Traits
Ear
ht, in
Moist%
ENO Hybrid
118-120
118-120
116-118
115-117
Traits
Days to
pollen
shed
13
Days to
pollen
Plant
ht, in.
Yield
tons/a
Yield
rank
shed
26
27
28
29
30
Integra 9676VTPRO
Integra 9651VT3
WXY10A
WXY10B
WXY10C
Wilbur-Ellis
Wilbur-Ellis
AgriLife (XU)
AgriLife (XU)
AgriLife (XU)
117
115
117
118
116
VT3PRO
VT3
None
None
None
69.0
69.0
71.0
70.0
69.0
112.3
104.7
119.3
116.0
108.9
45.8
36.1
50.9
45.1
37.7
56.3
58.7
56.9
61.9
60.0
32.82
30.53
29.88
28.89
31.93
8
22
25
30
15
31
32
33
WXY10D
WXY10E
WXY10F
AgriLife (XU)
AgriLife (XU)
AgriLife (XU)
118
117
118
None
None
None
71.0
70.0
71.0
113.4
114.4
115.1
57.0
43.4
45.7
65.1
58.6
61.9
32.44
26.42
29.78
11
33
27
69.8
0.8
0.9
114.7
3.4
6.3
45.7
10.0
7.5
58.7
3.5
3.3
31.36
5.88
3.01
Test mean
CV%
LSD 0.05
14