Microbiology 204
Antibodies: Flipped Session
TonyDeFranco and Chris Allen
October 15, 2014
Recommended Reading: Palm et al. (#11)
Reviews
1. Wu LC, Zarrin AA: The production and regulation of IgE by the immune system . Nat Rev Immunol 2014, 14 :247-259.
2. Yang Z, Robinson MJ, Allen CD: Regulatory constraints in the generation and differentiation of IgE-expressing B cells . Curr Opin Immunol 2014, 28 :64-70.
3. Macpherson, A.J., Geuking, M.B., McCoy, K.D. Homeland Security: IgA immunity at the frontiers of the body . Trends Immunol. 33: 160-167, 2012.
4. Hooper, L.V., Littman, D.R., Macpherson, A.J. Interactions between the microbiota and the immune system . Science 336: 1268-1273, 2012. (This is a very nice overview of key concepts and some key findings up to this time. It would be worth reading this toward the end of this course after we have covered more about T cell activation and effector types.)
Original Papers
1. Talay O, Yan D, Brightbill HD, Straney EE, Zhou M, Ladi E, Lee WP, Egen JG,
Austin CD, Xu M, et al.: IgE+ memory B cells and plasma cells generated through a germinal-center pathway . Nat Immunol 2012, 13 :396-404. a. Additional data in correspondence: Talay O, Yan D, Brightbill HD, Straney
EEM, Zhou M, Ladi E, Lee WP, Egen JG, Austin CD, Xu M, et al.: Reply to "On the differentiation of mouse IgE+ cells" . Nat Immunol 2012,
13 :623-624.
2. Yang Z, Sullivan BM, Allen CD: Fluorescent In Vivo Detection Reveals that
IgE+ B Cells Are Restrained by an Intrinsic Cell Fate Predisposition .
Immunity 2012, 36 :857-872.
3. He JS, Meyer-Hermann M, Xiangying D, Zuan LY, Jones LA, Ramakrishna L, de
Vries VC, Dolpady J, Aina H, Joseph S, et al.: The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response . J Exp Med 2013, 210 :2755-2771.
4. Hapfelmeier, S., Lawson, M.A.E., Slack, E., Kirundi, J.K., Stoel, M.,
Heikenwalder, M., Cahenzli, J., Velykorekko Y., Balmer, M.L, Endt, K., Geuking,
B.B., Curtiss, R., McCoy, K.D., Macpherson, A.J. Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses . Science 328: 1705-1709, 2010.
5. Slack, E., Hapfelmeier, S., Stecher, B., Velykorekko Y., Stoel, M., Lawson,
M.A.E., Geuking, B.B., Beutler, B., Tedder, T.F., Hardt, W-D., Bercik, P., Verdu,
E.F., McCoy, K.D., Macpherson, A.J. Innate and adaptive immunity cooperate flexibly to maintain host-microbiota mutualism.
Science, 325: 617-620 ,
2009
6. Fagarasn, S., Muramatsu, M., Suzuki, K., Nagaoka, H., Hiai, H., Honjo, T.
Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora . Science. 298: 1424-1427, 2002.
7. Suzuki, K., Meek, Doi, Y., Muramatsu, M., Chiba, T., Honjo, T., Fagarasan, S.
Aberrant expansioin of segmented filamentous bacteria in IgA-deficient gut . PNAS 101: 1981-1986, 2004.
8. Wei, M., Shinkura, R., Doi, Y., Maruya, M., Fagarasan, S., Honjo, T. Mice carrying a knock-in mutation of Aicda resulting in a defect in somatice

hypermutation have impaire gut homeostasis and compromised mucosal defense.
Nature Immunol. 12:265-270, 2011.
9. Lindner, C., Wahl, B, Fohse, L., Suerbaum, S. Macpherson, A.J., Prinz, I. and
Pabst, O. Age, microbiota and T cells shape diverse individual IgA repertoires in the intestine.
J. Exp. Med. 209: 365-377, 2012.
10. Kawamoto, S., Marua, M., Kato, L.M. Suda, W., Atarashi, K, Koi, Y, Tsutsui, Y.,
Qin, H., Honda, K., Okada, T., Hattori, M. and Fagarasan, S. Foxp3+ T cells regulate Immunoglobulin A selection and facilitate diversification of bacetial species responsible for immune homeostasis . Immunity 41: 152-
165, 2014.
11. Palm, N.W., deZoete, M.R., Cullen, T.W., Barry, N.A., Stefanowski, J., Hao, L.,
Degnan, P.H., Hu, J., Peter, I. Zhang, W., Ruggiero, E., Cho, J.H., Goodman,
A.L., Flavell, R.A. Immunoglobulin A coating identifies colitogenic bacteria in inflammatory bowel disease . Cell 158: 1000-1010, 2014.