Microbiology 551 CAPSTONE Schedule Spring 2015 The in-class research project is chosen each year and typically defines the microbiota of some environment using next generation sequencing technology (currently 454, moving to Illumina). This semester’s project is to define the microbiota of fermentations of biomass typically used in biofuel (ethanol) production. This was done by inoculating liquid media containing the desired biomass with bovine rumen fluid. The microbiotas is to be defined along with VFA HPLC analysis. The class meets formally for the first several weeks every time (3 hours, 2x a week), but then once a week for updates, and is an open lab other times for student to do work as needed. Proposal writing, poster preparation, bioethics and bioinformatics are also a major part of the course. Students can also do an out-of-class research project in a research lab. They will do the proposal writing, poster preparation and in class presentations. Project/Lab Work Date Day 1 1/21-22 W/R Introductions, expectations, organization etc. / Assign Groups / Brief presentations of the system 2 1/26-27 M/T Disc. of Protocol Writing Proficiency Exercise Disc. of Proposal Outline Writing Period 1-Ethics Intro 3 1/28-29 W/R Proficiency Exercise continued Proposal Outline Review in Class Paper Review (1 and 2) 4 2/2-3 M/T Proficiency Exercise continued Period 2 - Ethics 5 2/4-5 W/R Proficiency Exercise (2/6) Project Outline Due Paper Review (3 and 4) 6 2/9-10 M/T 7 2/11-12 W/R 8 2/16-17 M/T 9 2/18-19 W/R 10 2/23-24 M/T 11 2/25-26 W/R 12 3/2-3 M/T 13 3/4-5 W/R 14 3/9-10 M/T 15 3/11-12 W/R 16 3/16-17 M/T 17 3/18-19 W/R 18 3/23-24 M/T 19 3/25-26 W/R Project Work Proposal / Manuscript Bioethics / The System/Bioinformatics Period Lab Meeting Period 3 - Ethics Proposal Outline Due 2/13 Group Presentations Lab Meeting 454 Quiz Group Presentations Lab Meeting Proposal Draft Due 2/27 Lab Meeting Proposal Final Due 3/6 Lab Meeting Lab Meeting Lab Meeting 3/30-4/3 Poster Section Discussion Spring Break 20 4/6-7 M/T 21 4/8-9 W/R 22 4/13-14 M/T 23 4/15-16 W/R 24 4/20-21 M/T 25 4/22-23 W/R 26 4/27-28 M/T 27 4/29-30 W/R 28 5/4-5 M/T 29 5/6-7 W/R Lab Meeting Bioethics Opinion Paper Due Lab Meeting Bioinformatics Session 1 Bioinformatics Session 2 Lab Meeting Bioinformatics Session 3 Poster Due 4/24 Lab Meeting Lab Meeting