Instruction Working Title: Practical Problem-Solving with Punnett Squares Topic This instructional design will enable students with a basic prior introduction to the key terminology of heredity to accurately determine genotypes of offspring as part of the foundational study of genetics by utilizing a tool known as the Punnett Square. Learning Theory The design of this activity is based on the cognitive learning theory. The learners will utilize previously-studied terminology and apply the concepts to a real-world application. Throughout the lesson, learners will be required to synthesize knowledge and use it in a practical, problem-solving way. In addition, the exercises will involve collaboration with peers to arrive at solutions to the problems being presented. Problem Many freshmen students struggle with the fundamental concepts of genetics because of their mentality that the subject must be too complex for them to master, based on the newly-introduced terminology involved. This results in students having great fear of even attempting to solve genetics problems, due to their inability to determine how to organize the information in a manner that will allow them to solve the problem in a simple manner. Audience The audience for this exercise is freshmen-level students in a high school setting. Genetics is a required component of biology, which is one of the core science areas that all students must study and ultimately pass proficiency tests in order to graduate with a high school diploma at the end of their secondary educational journeys. Purpose The intention of this instructional design activity is to simplify the seemingly complex problem of determining the offspring of a genetic cross between two living things. Upon completion of the tutorial, students should have the core foundation to accurately predict the outcome of sexual reproduction by analyzing probabilities of the resulting genotypes after constructing a Punnett Square for the basis of this analysis. Format In this design exercise, learners will understand how to effectively create a Punnett Square using only instructions presented in the form of visual imagery. Students will not have access to any supplemental text or audio to aid them in completing the task. General Learning Expectations The learner will be able to construct a Punnett Square to predict all possible genotypes of the offspring of a monohybrid trait cross. Learning Goals and Objectives At the end of this instruction students will be able to successfully: G.1. Construct a Punnett Square and analyze the data included. O.1. Students will be able to identify genotypes of parents. O.2. Students will be able to arrange the genotypes of parents around the framework of a Punnett Square. O.3. Students will be able to work down columns and rows to “cross” the genes of each parent. O.4. Students will be able to determine the possible genetic combinations, or genotypes, of offspring. Learning Activities and Assessment 1. The learners will begin the assignment by locating their partners that have been assigned by the teacher. Students will work in pairs to complete the assignment. 2. The learners will then use the provided visual images for reviewing the basic concepts and examples associated with the problems to be solved. 3. The learners will use the provided visual cues as instructions and supportive background information to construct a Punnett Square that incorporates all the information needed to solve the task. 4. The alleles of parents will be represented using color-coded symbols, rather than letters. After constructing the Punnett Square, students will analyze the results to predict genotypes of offspring. 5. Upon completion, students will summarize their findings using only colorcoded symbols to represent offspring possibilities and present those to the instructor. A checkmark from the instructor will indicate an accurate analysis, and an “X” will mean students have arrived at an incorrect conclusion. 6. If learners successfully solved the problem on the initial attempt, the team will move on to the second genetics problem to solve. If not, the team will retrace their steps and attempt to solve the problem again and resubmit to the instructor for feedback. 7. Students must create at least three Punnett Squares and successfully analyze the results to receive full credit for the assignment and exhibit mastery of the concept. Completion Outcomes The primary outcomes of the instruction include: 1. Distinction between dominant and recessive traits 2. Successful construction of a Punnett Square 3. Accurate analysis of a Punnett Square to solve a problem 4. The ability to correct any mistakes made in the initial attempt and to repeat the problem-solving technique twice, indicating mastery Final Product: Submitted Problem Solutions Students will submit the three completed Punnett Squares with offspring analyses indicated by color-coded symbols recorded on notebook paper. Technology required Learners will have access to computers and/or mobile devices to view the visual aids for the assignment. Learners will have access to an internet connection to access the image cues. Learners will have paper, colored pencils, and writing utensils to record results. Timeline Students will have 5 minutes to locate their assigned partner and gather supplies. Students will then study the background example diagrams for 10 minutes, discussing the sample information together for better understanding. After reviewing the background information, completion of the exercises and analysis/feedback of the problem-solving process should take no more than 10 minutes per Punnett Square creation/problem-solving attempt. The total time for gathering supplies, reviewing example information, solving problems, and receiving teacher feedback should be 45 minutes. Assessment Rubric Rubric for Practical Problem-Solving with Punnett Squares Exercise Design, Process, Feedback Students: Students demonstrate proper design of each Punnett Square. Total /15 Students correctly identify and label the genotypes of the parents being crossed with the proper colorcoded symbols above the columns and to the left of the rows of each Punnett Square. /10 Students accurately record the possible genetic combinations of the offspring resulting from the cross of the parents with the proper color-coded symbols in the correct columns/rows of each Punnett Square. /20 Students work collaboratively to successfully determine the possible genotypes of the offspring, analyzing information from the Punnett Squares. /15 Students present the solutions to the instructor using only color-coded symbols for feedback on the analysis of the Punnett Squares. /10 Students complete at least three Punnett Squares, reworking their solutions as needed, until receiving confirmation of the correct solutions to each problem from the instructor. /20 Students submit completed Punnett Squares and analyses to instructor on notebook paper. /10 Bonus: Students successfully solve all problems correctly on the first attempt. /5 Total points General feedback: /100 Evaluation Self-Assessment Do you feel you learned how to successfully construct a Punnett Square? Did you find using the Punnett Square as a problem-solving tool simplified the process of determining the genotypes of the offspring? Team Assessment Do both members of your team feel that you learned and accomplished the objectives by working together in a productive way? Were the visual instructions clear enough to help you determine the layout of a Punnett Square on the first attempt? What could have been added to the instructions that may have made it a more simple process to complete and aid in your understanding of the tasks involved in the activity?