Illumina HiSeq2000/2500 and MiSEQ Sequencing Sample Submission Form University of Colorado Anschutz Medical Campus 1. Contact Information PI Name Post Doc/Student/Tech Institution Address City PI Email Email Phone State Zip Code 2. Experiment and Sample Information 2.1 If you are submitting DNA/RNA samples for sequencing library preparation, please indicate sequencing type. The optimal quantity of starting material is listed below. If sufficient RNA/DNA quantity and concentration is not available, please consult the Core personnel before submitting. Note: additional 100 to 200 ug of samples are required for sample QC/QA. Genomic DNA Seq 1ug Genomic DNA (1ug to 3ug) in 50ul water/TE buffer or less Small RNA Seq 1ug Total RNA (1ug to 5ug) in 5ul water/TE buffer or less mRNA Seq 500pg to 100ng (Low input RNA library prep in 10ul of water/TE buffer) ChIP Seq >1ng ChIP DNA in 10ul water/TE buffer or less Exome Seq 1ug Genomic DNA in 50ul water/TE buffer ( can be done at 200ng if necessary) Directional mRNA Seq 1ug (500ng minimum) Total RNA in 50ul water/TE buffer or less Directional Total RNA Seq Ribo Zero 1ug Total RNA (0.1ug to 4ug) in 10ul water/TE buffer or less Other / Custom Please attach / list below sample QA/QC data if any (Nano Drop, Pico Green, QPCR, Qubit, Bioanalyzer, etc) 2.2 If you are submitting ready-to-run sequencing libraries, please indicate the type of library. Genomic DNA Seq Small RNA Seq mRNA Seq Directional mRNA Seq Directional Total RNA Seq Ribo Zero ChIP Seq Exome-Enrichment DNA Seq Other Please Indicate Length and Location of Tag: Tag Sequence Length: (example 6 base pairs) _____________________ Dual Index: __________Yes _______________ NO Location of Tag: __________ Read 1 ___________ Index Read ____________Read 2 Other Tag Information we need to know: _________________________________________________________ ___________________________________________________________________________________________ Are Custom Primers Required: ___________YES (Explain how to use them to us below) ___________ NO ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ Please indicate what kit you used for sequencing library preparation: ________________________________________________________________________________________ Please attach library QA/QC data if any (Nano Drop, Pico Green, QPCR, Qubit, Bioanalyzer, etc). PLEASE NOTE: It is customer’s responsibility to ensure your library is Illumina sequencing compatible. We will do our best to get you the highest number of reads per lane, however there is no read number guarantee for customer made sequencing libraries. 2.3 Sample information 1 2 Species of samples submitting Sample names and concentration (if available) in the box below.(Add more rows if needed) Sample Name Concentration Tag Sequence (if any) 2.4 Choose a sequencing run option below. HiSEQ 2000/2500 HT Mode V3 Chemistry (Average 150 M reads per lane for single read sequencing) _____ Single Read 50 cycles (1x50) _____ Single Read 100 cycles (1x100) _____ Paired End Read 100 cycles (2x100) HiSEQ 2000/2500 HT Mode V4 Chemistry (50% more reads than V3 Chemistry –costs more) _____ Single Read 50 cycles (1x50) _____ Single Read 125 cycles (1x125) _____ Paired End Read 125 cycles (2x125) HiSEQ 2500 Rapid Mode 2 Lane Sequencing (Must buy 2 Lanes) _____ Single Read 50 cycles (1x50) _____ Single Read 100 cycles (1x100) ______ Single Read 150 cycles (1x150) ______ Paired End Read 150 cycles (2x150) MiSEQ _____ Single Read 50 cycles (1x50) V2 Chemistry _____ Paired End Read 150 cycles (2x150) V2 Chemistry _____ Paired End Read 250 cycles (2x250) V2 Chemistry _____Single Read 150cycles (1x150) V3 Chemistry _____Paired End Read 300 Cycles (2x300) V3 Chemistry CUSTOM RUN: OTHER RUN OPTION State Machine HiSEQ 2000, HiSEQ 2500 or MiSEQ and Run Length Desired ________________________________________________________________________ 2.5 How many flow cell lanes do you plan on using? If multiplexing samples in a lane, please group your samples. Lane Samples Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 2.6 Data analysis/bioinformatics options On our own (Email of person who will receive data) ______________________________________ By a designated bioinformatician Bioinformatician Name: By UCD Bioinformatics Core Email: 3. Payment Information 3.1 University of Colorado Faculty Speed Type CCTSI or Cancer Center Member ___Yes ___No 3.2 Not Affiliated with the University of Colorado An additional fee of 7.2% of total bill will be added to your invoice to cover University Associated Billing Fees _______ Payment by Check _______ Payment by Credit Card _______ Payment by Wire Transfer 4. Sample Drop off or by Shipping Email or call to schedule a time for sample drop off. Contact Contact Hours of operation for sample drop off Location/ Shipping Address Katrina Diener Katrina.diener@ucdenver.edu; Microarray.core@ucdenver.edu (303) 724- 6050 Todd Brian.Woessner@ucdenver.edu Woessner (303) 724-6047 9:00am to 3:00pm Monday – Friday, excluding university holidays. Genomics and Microarray Core University of Colorado Anschutz Medical Campus 12700 E. 19th Ave Bldg: RC-2, Room 9400 Aurora, CO 80045 5. Required Signatures Please have both the Principle Investigator (PI) that will be paying for the Sequencing Services and the Research/Technician/Student/ Post-Doc preparing the samples sign below acknowledging that all of the information provided on the form is correct. Signature of this form acknowledges that the PI and Technician/Student/Post-Doc have agreed to all sample submission, quality, quantity, project scheduling, and researcher financial responsibility requirements. Signature of this form authorizes the UC Genomics and Microarray Core to order all consumables necessary for the researcher’s sequencing project and confirms that the PI is financially responsible for items ordered for their project and all labor cost associated with the project. Principle Investigator Signature Date Technician/Student/Post-Doc Signature Date 6. Data and Sample Retrieval All data from sequencing run will be deleted from our servers 31 days after you receive notification that your data is ready to be downloaded from our server via email. Please pick up original DNA / RNA submitted along with constructed libraries no later than 60 days after data has been downloaded from our server. If you do not pick up your samples and libraries they will be destroyed.