Table S3a Predicted apple gene models with significant similarity to MADS8 cDNA sequence. Similarity classed as greater than 100bp in length and greater than 85% pairwise identity. Genome Database for Rosaceae MDP number1 MDP0000366022 MDP0000366019 MDP0000189308 MDP0000289858 MDP0000289858 MDP0000605482 MDP0000508777 MDP0000326390 MDP0000326390 MDP0000220008 MDP0000280009 MDP0000149676 MDP0000326906 MDP0000489384 MDP0000574222 MDP0000126997 1 Pairwise % identity 99.0 94.0 94.0 94.0 97.0 95.0 100.0 85.0 81.0 84.0 86.0 85.0 85.0 85.0 85.0 85.0 Region of identity (bp) 757 642 518 471 186 429 146 228 116 212 173 171 173 167 167 162 Score (bits) 1485 979 789 755 337 696 289 182 63.9 159 153 149 145 141 141 139 E-value 0 0 0 0 4.00E+91 0 8.00E-77 1.00E-44 8.00E-09 2.00E-37 1.00E-35 2.00E-34 3.00E-33 4.00E-32 4.00E-32 2.00E-31 Gene name MADS8 MADS9 MADS9 MADS9 MADS9 MADS9 MADS8 MADS7 MADS7 MADS118 MADS104 MADS11 MADS4 MADS6 MADS6 MADS4 Consistently down-regulated? Yes Yes Yes Yes Yes Yes Yes Partially Partially No No No No No No No Individual loci often have more than one gene model Greatest length of uninterrupted identity (bp) % identity of region Identities within region (bp) Region of identity (bp) Table S3b Apple genomic regions with significant similarity to MADS8 cDNA sequence. Regions identified had one or many regions of 21bp or greater with 100% identity. Representative Gene Model and corresponding apple MADS-box gene (if named) Apple genome linkage group and region of identity Regions with ≥21bp continuous, uninterrupted identity MDP0000366022 (MADS8) LG17:5246491-5252764 1231 1216 98.8 386 3 MDP0000366019 (MADS9) LG9 :4226116-4231709 1125 1066 94.8 141 MDP0000149676 (MADS11) LG2:23947679-23947849 171 147 86.0 22 LG2:38310483-38310522 40 38 95.0 23 MDP0000156783 MDP0000326906 (MADS4) LG131:3736633-3736794 162 139 85.8 30 MDP0000220008 (MADS118) LG16:6507031-6507206 176 149 84.7 26 MDP0000370413 (MADS18) LG13:8462372-8462547 176 148 84.1 26 1 LG7 :1746003-1746042 40 37 92.5 23 MDP0000254052 LG43:268711-268754 44 40 90.9 23 MDP0000209705 Regions with ≥21bp continuous, uninterrupted identity but with no gene model or EST support LG162:18089604-18089625 22 22 100.0 22 no gene model at loci 1 similarity was identified on alternative haplotype 1 of the linkage group stated 2 similarity was identified on alternative haplotype 2 of the linkage group stated 3 similarity was identified on alternative haplotype 3 of the linkage group stated Arabidopsis gene with greatest similarity SEP1/2 SEP1/2 AGL6 AGL15 SEP4 SEP3 SEP3 AGL15 AGL22