Course flyer 2014-11

Novel fluorescence-based methods in immunology
PhD student course, KI, 9th – 13th of February 2015
The course will introduce different state-of-the-art fluorescence-based techniques
relevant to immunological research, including super-resolution microscopy, the
development of which was awarded by this year’s Nobel prize in Chemistry!
The techniques presented will address spatial and temporal dynamics at the cellular
and molecular level. At the cellular level, techniques to study cell migration in vitro
and in vivo. At the molecular level, measure diffusion and quantitate concentrations
of molecules in live cells (FCS), the interaction between two molecules and
conformational states (FRET), and finally localization of proteins down to single
molecule level (super-resolution microscopy).
At the end of the course, the students will be able to give an account of available
state-of-the art fluorescence-based techniques to investigate different research
questions within immunology. This includes an ability to compare and discuss the
relative advantages and disadvantages of the different techniques, and in which
situations the use of each one of them is appropriate. The students should be able to
relate these limitations and benefits to their own research project. They should also be
able to formulate and ask relevant questions about the different techniques to experts
of the respective techniques.
Register before November 17th!
Course page:
The course also includes participation at the 2-day national symposium with the same
name, February 9-10th at the Karolinska Institutet:
For questions regarding the course, contact Sofia Johansson: