2015-2016 Funded Research Abstracts TITLE: Developing improved winter wheat cultivars for Oregon INVESTIGATOR: Robert Zemetra Crop & Soil Science Department Oregon State University (OSU) FUNDING HISTORY: 2012-2013 - $299,525 2013-2014 - $300,663 2014-2015 - $300,000 APPROVED FUNDING: $240,000 ABSTRACT: Essential to the economic survival of the Oregon wheat producers is the development of new soft white, hard white and hard red winter wheat cultivars with improved yield potential, improved biotic and abiotic stress resistance/tolerance, and superior end-use quality. To achieve this goal the wheat breeding program will utilize a combination of classical and molecular breeding techniques. Diverse sources of germplasm will be utilized to produce new gene combinations for selection. Early generation material (F1 – F4) will be evaluated in the field in both western and eastern Oregon to identify lines with disease resistance, abiotic stress tolerance, high yield potential and good end-use quality. Complimenting the field testing will be evaluation at the molecular level to identify lines carrying desired genes for traits such as disease resistance and abiotic stress tolerance to pyramid multiple resistance genes within the same genotype. Intermediate generation material (F5 – F6) will be evaluated in preliminary yield trials for yield, agronomic adaptability, disease resistance and milling quality. Lines will also be tested in the greenhouse and field for abiotic stress tolerance such as aluminum toxicity associated with low pH soils. Advanced generation material (F7 – F8) will be evaluated at multiple locations in the state for agronomic adaptability, disease resistance/tolerance, yield stability and milling and baking quality. Elite lines (F9 – F10) will be provided to Mike Flowers for evaluation in the Oregon Winter Elite Yield Trial (OWEYT) and Hard Winter Elite Yield Trial (HWEYT) to identify superior lines for release as cultivars. Elite lines will also be entered into regional testing and the extension trials in Washington and Idaho for evaluation under additional climatic conditions. Prior to release, potential cultivars will be submitted for evaluation by the Pacific Northwest Wheat Quality Council to confirm the lines have the desired end-use quality for that market class. In addition to the breeding research, specific projects will continue on development and utilization of molecular markers in soft winter wheat and hard winter wheat. Breeding efforts will continue on the development of improved herbicide resistant wheat cultivars. Project: Oregon Barley Variety Development and Deployment Investigator: Patrick Hayes. Dept. of Crop and Soil Science, Oregon State University, Corvallis, Oregon 97333 1 Funding History: FY2014: $38,000; FY 2013: $46,000; FY 2012: $20,000 Approved Funding: $36,900 Abstract: We propose to develop and deploy barley varieties for Oregon. We will continue development and statewide testing of malting, feed, food, and forage varieties with an emphasis on facultative and winter growth habit. We will expand spring-planted testing of potential varieties targeted to the craft industry. We will conduct a second year of winter feed barley trials in cooperation with Larry Lutcher in Morrow county with the goal of identifying winter hardy varieties suitable for low rainfall areas. New this year is a collaborative effort with OSU colleagues at key irrigated locations around the state to conduct large-scale spring 2-row malting variety trials with the goals of providing data on agronomic and brewing performance. Oregon is not only a world leader in craft brewing and distilling, but also has the potential to develop strong commercial and craft malting industries – and to create a true “all-Oregon beer”. In addition to a strategically located commercial malting facility (Great Western Malting), Oregon has a developing craft malting industry with two malt houses scheduled to start production in 2015. Oregon growers are ideally positioned to provide these industries with barley, but additional variety and management information is needed to ensure the integrity and superiority of the Oregon “brand”. The craft malting industry has lower specifications for grain protein (8 – 12%) and protein-related malting quality traits than the commercial industry. The focus this year is on three irrigated environments: Klamath, Linn, and Union counties. The data we generate will allow stakeholders to make agronomic, malting, and brewing decisions based on variety and nitrogen management. In order to generate sufficient grain for malting and brewing we will produce 200 or more pounds of grain per variety/nitrogen combination at each location. Therefore, this is a larger-than-average trial. Funding is requested from the OWC for the agronomic testing component of the proposal, with supplemental funding provided by the participating industry partners for malting and brewing of selected samples. Title: Wheat and Spring Barley Variety Testing in Oregon Investigator: Michael D. Flowers, Associate Professor and Extension Cereal Specialist, Crop and Soil Science Department, Oregon State University (OSU), Corvallis Funding History: 2012 – 2013 - $100,363, 2013 – 2014 - $79,050, 2014 – 2015 - $63,900 Approved Funding: $73,182 Abstract: The Oregon statewide variety testing program provides growers with performance information on commonly grown and newly released wheat and spring barley varieties from the public and private breeding programs. Wheat varieties are split into three categories (Oregon Winter Elite Yield Trial or OWEYT; Hard Winter Elite Yield Trial or HWEYT; and Oregon Spring Elite Yield Trial or OSEYT) for evaluation. Spring barley varieties are evaluated in the Oregon Spring Barley Variety Trial (OSBVT). Each year the testing program has approximately 17 winter and eight spring locations throughout Oregon, eastern Washington, southeastern Idaho, and northern California. The southeastern Idaho and northern California trials are done in collaboration with Idaho and California extension programs, respectively. Trial locations are chosen to capture a range of environmental conditions and/or cropping systems (such as no-till) in the wheat production areas of Oregon. Trial results are reported through email alerts, web publications, grower meeting, crop tours, and field days. TITLE: Oregon State University Cereal Quality Laboratory PRINCIPAL INVESTIGATORS: Dr. Andrew S. Ross, Dr. Teepakorn Kongraksawech: Crop & Soil Science, Oregon State University (OSU) FUNDING HISTORY: 2012-13: $76,515; 2013-14: $78,548, 2014-15: $81,872, 2015-16 -Requested $88,600 APPROVED FUNDING: $86,322 ABSTRACT: Over the last decade the OSU cereal quality laboratory (CQL) has provided documented benefit to Oregon growers by improving the quality of Oregon State soft-white wheat varieties. This was achieved by extensive testing based on three core objectives: selecting appropriate lines for the breeding program, increasing our fundamental knowledge of grain composition and functionality, and improving our analytical methods. During 2014 the CQL processed in the order of 13000 analyses at a cost of ~ $6 per analysis, based on 2014/15 funding. Other funds supported our OWC mission: Wheat royalties, the Nixon Family of Junction City OR, and the Mexican National Council of Science and Technology (1 PhD student). Looking to 2015-16, the following specific objectives support our core objectives. 1: Complete the current phases of these studies: Falling Number, gluten proteins in varieties grown over the last 110 years, cakes and cake batters, and validating the prediction of dough strength using a rapid screening technique. The batter and Falling Number studies encompass ongoing studies of grain and flour storage (aging). We propose to apply new technology to observe gluten protein behavior during processing, with the first task providing fundamental information to support the soft-wheat blending project. We will commission and validate the “SRC-Chopin” robot for automating an essential quality test for the breeding program. We propose to initiate investigations into frozen doughs and crackers to improve breeding outcomes for these products. We will also participate in a PNW-wide inter-laboratory study aimed validating our laboratory-milling protocols. We have recently completed studies on pancake quality, the genetics of unexpectedly good quality in a hard x soft cross, two studies on oxidative gelation in soft wheat, and study of the influence of fungicides on Falling Number. Project Title: Transition of Pre-breeding Research on Disease Resistance and End-Use Quality into Cultivar Development for Oregon Wheat Investigator: Robert S. Zemetra, Department of Crop and Soil Science, OSU Previously investigated by J. Leonard. Funding History: 2012-2013, $46,821; 2013-2014, $46,200; 2014-2015, $34,135 Funding Requested: $36,000 Abstract: Previous research by the wheat molecular genetics laboratory (WMGL) has introgressed genes for improved disease resistance and end-use quality into adapted Pacific Northwest (PNW) germplasm. The next step is to utilize these genes in the cultivar development program by introgressing the genes into elite soft white winter and hard white winter wheat breeding lines and cultivars. The proposed research will focus on continuing the transfer of resistance gene(s) for Cephalosporium stripe resistance into soft white winter wheat breeding lines and the transfer a null Polyphenol Oxidase (PPO) gene and an enhanced glutenin gene into hard white winter and hard red winter wheat breeding lines. This research will entail using molecular markers, cytogenetic analysis and field evaluation. Title: Oregon Wheat Statewide Weed Management Research and Extension – 2015-2016 Investigators: Andrew G. Hulting, Extension Weed Management Specialist, Corvallis Carol Mallory-Smith, Weed Research Project Leader, Corvallis Funding History: 2012-2013 - $58,740; 2013-2014 - $61,070; 2014-2015 - $55,935; Approved Funding: $63,150 Abstract: We will conduct a coordinated program to test herbicides for use in wheat across statewide environments thereby facilitating a more rapid registration and statewide labeling for all Oregon wheat production systems. Because of the extreme diversity between eastern and western Oregon wheat cropping environments and spectrum of weed species across the region, it is necessary to test new compounds in both regions before a complete data package can be submitted to EPA for statewide product registration. All of the major herbicide manufacturing companies collaborate with the investigators listed on this proposal and rely on their data to successfully obtain new herbicide registrations for Oregon wheat. However, chemical company funding alone is not adequate to maintain the OSU program infrastructure necessary to carry out year-to-year cooperative efforts for herbicide evaluation. In addition, we will conduct more in-depth studies to determine best methods for management of problem weed species and biotypes in wheat including scouring rush and multiple resistant Italian ryegrass. New information derived from statewide weed research will be disseminated to Oregon wheat growers and consultants using an array of Extension programming methods. TITLE: Impacts of mixed cereal-oilseed cropping systems on weed communities in the PNW INVESTIGATOR: Dr. Judit Barroso, OSU-Columbia Basin Ag. Research Center, Pendleton & Moro FUNDING HISTORY (last 3 years): None APPROVED FUNDING: $30,130 ABSTRACT: Glyphosate is an effective herbicide in the inland Pacific Northwest region. However, over reliance on this chemical has led to the occurrence of herbicide resistant weeds. For example, glyphosate resistant Italian ryegrass is now common in Oregon (Perez-Jones et al, 2014) and resistant Kochia has been documented as well (Felix, 2014). Decreases in the retail price of glyphosate have encouraged producers to increase applications of this chemical, particularly in farm fields that receive direct seeding, thereby further increasing the potential for glyphosateresistant weed species. Diversification of crop rotations may reduce the abundance of dominant weed species (Liebman & Dyck, 1993) and decrease reliance on herbicides to control weeds. Cropping diversification as a best management practice for controlling weeds (and preventing weed resistance to glyphosate) has not been adequately assessed in dryland wheat production systems of the inland PNW. We will determine the influence of four different cropping systems: 1) winter wheat – summer fallow, 2) winter wheat – reduced tillage fallow, 3) winter wheat – spring barley – reduced tillage fallow, and 4) winter wheat – spring oilseed – reduced tillage fallow and the associated weed management practices on weed population dynamics such as changes in the abundance, diversity, and composition of weeds over time. Specifically, we will determine whether: 1) reduced tillage fallow, which increases the water available in the seed zone for winter wheat, will enhance stand establishment thereby increasing crop competitiveness against weeds, 2) spring barley and spring oilseed crops in rotation with winter wheat reduces competitiveness of economically important weeds such as Russian thistle and downy brome, and 3) the level of weed density above which weed management is profitable (e.g., economic threshold). Impacts of weeds on winter wheat, spring barley, and spring oilseed yields will be estimated with and without herbicide treatment. Crop specific, curvilinear models will be developed to indicate the economic threshold (ET) where the cost of control equals the net economic benefit. TITLE: Screening for Resistance to Major Wheat Diseases in Oregon INVESTIGATOR: Chris Mundt, Botany and Plant Pathology, Oregon State University (OSU), Corvallis FUNDING HISTORY: $43,838 for 2012-2013; $44,599 for 2013-2014; $42,630 for 2014-15 APPROVED FUNDING: $48,731 ABSTRACT: A combination of locations, production practices, and inoculation techniques will be used to provide high levels of disease pressure in trials of stripe rust, Cephalosporium stripe, Fusarium crown rot, strawbreaker foot rot, Septoria tritici blotch, and barley yellow dwarf virus. Resistance levels of entries in elite and advanced yield trials will be determined to evaluate potential varietal releases and to allow growers to make the best varietal decisions when new varieties are first available to them. Molecular mapping of three populations with potential to contribute to the next generation of disease resistant, higher yielding winter wheat varieties for Oregon growers will be completed. A new mapping population, Madsen x Foote, will be grown to evaluate the genetics of durable stripe rust resistance in Madsen and to evaluate and combine genes for quantitative resistance to Septoria from Madsen and Foote. Improved markers will be developed in two populations via genotyping-by-sequencing. A field trial will be established to test Oregon wheat varieties for ability to suppress build-up of the take-all pathogen, based on promising research that has recently been reported from England. The overall project will result in data collection from 12,000 to 16,000 plot observations. The studies are crucial to continued progress in the OSU Wheat Breeding Program, increased profitability for Oregon wheat growers, and ability to adopt conservation tillage practices. Title: Developing applications of on-combine optical sensing for precision nitrogen management of dryland wheat production systems in eastern Oregon Investigator(s): Stephen Machado, OSU-CBARC Pendleton Funding History: 2011-2012: $32,000 for “The Status of Soil Organic Carbon on Eastern Oregon Fields under Summer Fallow Project”. Approved Funding: $50,000 Abstract: Traditional uniform crop management ignores inherent spatial variability in topography, soil type, soil depth, soil moisture, soil fertility, weeds and diseases within fields resulting in over application of crop inputs in some areas and under application in others. By accommodating this variability, site-specific management (SSM) has the potential to improve cropping input efficiency and farm profits. To implement SSM, prescription maps are often constructed from soil maps, aerial imagery, electrical conductivity, grain yield maps, and other source documents to divide fields into smaller areas that differ in crop productivity and can receive different levels of fertilizer and other farm chemicals. Unfortunately, there is no single method available that can be used reliably to accurately derive these maps, particularly in semiarid conditions in eastern Oregon where water is the most important factor limiting crop yields. The objectives of this study are to (1) evaluate the performance of an advanced optical sensor for measuring the protein concentration of soft white wheat as it is harvested on a combine; (2) adapt an affordable, off-the-shelf spectrometer for use on a combine harvester and compare its performance with the advanced sensor; (3) map grain yield, grain protein, and straw yield from the combine during harvest, and apply this information into (a) construction of management zones for precision nitrogen application in a following year, (b) post-harvest assessment of water/nitrogen stress, and (c) mapping of weeds at harvest by detecting the concentration of chlorophyll in the grain stream while harvesting; and (4) transfer knowledge of multi-sensor, on-combine data collection to wheat producers by means of workshops, field days, conferences, and field demonstrations. A 19-acre spring wheat field will be spatially characterized for soil depth, bulk electrical conductivity (EC), soil physical and chemical properties, topography, soil-borne diseases, and weeds using various mapping techniques. A ruggedized Polytec 1720 spectrometer with an advanced InGaAs detector will be mounted to a Case IH combine and the spectra obtained will be used to estimate the protein concentration of the grain stream during harvest. Grab samples of grain will be analyzed in the laboratory and compared with the on-combine measurements. Affordable, off-the-shelf spectrometers will be tested both on a test stand and on-combine to determine sensor mounting requirements and ability to measure protein of conveyed grain. At end of season, grain protein, grain yield, and straw yield will be measured using an on-combine, multi-sensor data collection system consisting of mass flow and optical sensors. The goal of this study is to identify simple and cost effective ways of applying on-combine information into precision N management, weed/disease mapping, and interpretation of crop yield variability. TITLE: Base Funding for OSU Wheat Research Programs PROGRAM MANAGER: Jay Noller, Head, OSU Crop and Soil Science FUNDING REQUEST: $265,000 Given financial conditions in the State of Oregon and the United States, base funding for Agricultural Experiment Station (AES) and Extension programs has been cut significantly over the past decade. Reductions in funding have reached a point where continuation of some AES and Extension activities is not be possible without identification of other funding sources. Accordingly, the Oregon State University (OSU) College of Agricultural Sciences (CAS) has asked its clientele groups to provide base funding for activities in which they have interest. Base funding provides dollars for staff salaries and facilities operation support, areas for which clientele groups historically did not provide funding. Funding sources identified by clientele to date have included funds directly contributed by commissions and associations, from special tax districts, and through endowments. The Oregon Wheat Commission has been a long-time partner in wheat research activities at OSU and has provided base funding over the past several years and agreed to provide $265,000 in base funding for 2015-16. Russ Karow (former CSS department head) and experiment station directors from the cereal-related stations across the state –Clint Shock (Malheur Experiment Station), Valtcho Jeliazkov (Columbia Basin Ag Research Center), Phil Hamm (Hermiston Ag Research and Extension Center), Carol Tollefson (Central Oregon Ag Research Center), and Willie Riggs (Klamath Basin Research and Extension Center) conferred and established an agreement that allocates available OWC base funding. Two-thirds of funds are to be allocated to CBARC given its dominance in and near complete focus on cereal work. MES receives a lesser amount of funding given that a smaller amount of cereal work is being done at MES at this time. Remaining funds are to be split equally among COARC, CSS, HAREC and KBREC.