DEPARTMENT OF BIOLOGY AND CHEMISTRY
GUIDELINES FOR ANIMAL EXPERIMENT
BACKGROUND
Teaching and research experiments involving living vertebrate animals can be performed
only by staff and students who are licensed under the Animals (Control of Experiments)
Ordinance, Cap. 340. A license specifies the type of experiment; type of animal used and
place(s) where experiment may be conducted. Any person who contravenes the ordinance
shall be guilty of an offence and liable on summary conviction to a fine of one thousand
dollars and to imprisonment of six months.
Principal Investigators are required to:
 Follow the “Guidelines on Ethical Review of Research Experiments Involving
Animal
Subjects”
from
Research
Committee
of
the
University,
http://www.cityu.edu.hk/ro, the Guidelines is listed in Appendix I
 Submit an “Application for ethical review of research experiments involving animal
subjects” to Research Committee of the University, the application is listed in
Appendix II
 Apply a license for doing any experiment involving living vertebrate animals, by
downloading the form from the website of the Department of Health (as listed in
Appendix III):
http://www.dh.gov.hk/english/useful/useful_forms/useful_forms_ani.html
o Type in all necessary information into the form (Form 1) and the Annex
o Obtain a department chop from BCH Chief Technical Officer or Senior
Technical Officer
o Return the form to Department of Health by mail, fax or email:
Office Address:
Telephone number:
Fax number:
E-mail:
Special Health Services
Department of Health
Room 79, 21/F, Wu Chung House
213 Queen’s Road East
Wan Chai
HONG KONG
(Re: Application for License under the Animals
(Control of Experiments) Ordinance)
29618975
21277329
ro_al@dh.gov.hk
In case of doubt on whether a license is required, advice of the licensing authority should
be sought. For teaching experiment, ‘Bloc License’ should be obtained from the
Department of Health, by the teaching staff in charge, to cover the group of named
students involved in individual experiment(s).
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
Conduct a risk assessment on your experiment(s) (with risk of handling clinical
waste and the control measures), risk assessment form can be access from:
http://www6.cityu.edu.hk/cdfo/default.aspx?PageID=form&Lang=eng
Principal Investigators are requested to send a copy of the ethical review, animal license &
risk assessment to the Animal Facility Technical Officer-In-Charge for central filing.
TRAINING AND INDUCTION
 New staff should contact the Animal Facility Technical Officer-In-Charge (TO-InCharge) to take an induction in animal handling techniques which involve viewing
a training video, briefing by the Technical Officer-In-Charge followed by a short
written test
 Use appropriate personal protective equipment (e.g. safety goggles, gloves and
laboratory coat) at all times
PREVENTIVE MEASURES FOR ANIMAL WORKS
 Bites and scratches
o Receive proper training
o Wear anti-bite gloves (provided by Department) when handling live animals
o Wear surgical gloves, lab coat and safety glasses during dissections and
examinations
o Immunization against tetanus is strongly recommended
o Report as soon as practicable to the laboratory staff, supervisor and
Department Head for any bite, scratch or similar injury. Medical advice and
subsequent supervision may be needed if an infected animal inflicted the
injury

Zoonotic Diseases (disease that can be transmitted from animals to humans)
o Acquire the animals from reliable sources
Links of Animal Centers which supply animal to local universities:
HKU
http://www.hku.hk/launit/
CUHK
http://www.lasec.cuhk.edu.hk/
HKUST
http://ihome.ust.hk/~webacf/
o Perform animal manipulations in ventilated biosafety cabinets
o No eating, drinking, or apply cosmetics or contact lenses in laboratory
o Wear gloves when handling animals or their tissues
o Not to accidentally rub the face with contaminated hands or gloves
o Wash hands after each animal contact

Allergies
o Workers may show signs of allergies due to exposures to animals on contact
during feeding, cleaning, dosing sacrifice, surgery, and body fluid collection
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o Symptoms include sneezing and runny nose. More serious reactions include
cough, chest tightness, wheezing, or shortness of breath
o Workers who report symptoms of work-related asthma should be medically
monitored for early intervention
o Take following steps to prevent exposures:
 Perform animal manipulations in ventilated biosafety cabinets
 Avoid wearing street clothes while working with animals, minimum
protection gloves and lab coat should be worn
 Leaving working clothes at the workplace to avoid potential exposure
problems to family members
 Train workers in recognizing the signs and symptoms of allergic
reactions may prevent further asthma development

Chemical
o Treat anesthetic agents used in laboratory animals as hazardous chemicals
with a risk assessment carried out of the chemical agents and the operations
involved
o Refer to material safety data sheet (MSDS) for the chemical properties of the
chemicals used
o See http://www6.cityu.edu.hk/cdfo/download/chemicalwastedisposal.pdf for
the University policy on the disposal of chemical waste

Waste Disposal
o Dispose of the bedding and carcasses separately
o Seal the bags containing waste with ‘swan neck’ method
o Bedding is discarded as general waste while carcasses are stored in
designated freezer before they are collected by authorized clinical waste
collector
USERS’ RESPONSIBILITY
 Follow the Regulations listed in Appendix IV and V
 Report any malfunction or facility damage to Animal Facility Technical Officer-InCharge
 Users are encouraged to work during regular hours (9:00am – 5:30pm) and not to
work alone. If you are working outside of these hours or on weekends, you should
notify someone of your whereabouts and your expected time of return. No student
helpers and undergraduate final-year-project students can work alone in the
Animal Facility rooms. They should be accompanied by a postgraduate student or
staff at all times
 Users who need to work after 11:00pm or overnight in laboratory have to inform
CDFO at x8888 latest by 5:00pm on the same day. Users have to collect a personal
alarm from Ms. Helen Ng, Chief Technical Officer (ext 4080), so as to get
emergency help if required
 Report all incidents and accidents immediately, irrespective of their seriousness to
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CDFO Security (x8888), Animal Facility Technical Officer-In-Charge, Supervising
Academic Staff, and Laboratory Safety Officers
STAFF INFORMATION
BCH Biological Safety Officer &
Animal Facility Staff-In-Charge
Dr. Lam Yun Wah
Telephone: 34426347
Email: yunwlam@cityu.edu.hk
BCH Animal Facility Officer-In-Charge
Miss Mok Oi Lam Helen
Telephone: 34422030
Email: bhholmok@cityu.edu.hk
BCH Safety Officer & Chief Technical
Officer
Ms. Ng K Y Helen
Telephone: 34424080
Email: bhhelen@cityu.edu.hk
BCH Senior Technical Officer
Mr. Shum K W Eric
Telephone: 34424087
Email: bheric@cityu.edu.hk
EMERGENCY TELEPHONE NUMBER
CDFO Security Hotline
Extension: 8888 (24 hours emergency)
CDFO Helpdesk Hotline
Extension: 8833 (Malfunction of electricity
and water supply)
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Appendix I
CITY UNIVERSITY OF HONG KONG
Research Committee
GUIDELINES ON ETHICAL REVIEW
INVOLVING ANIMAL SUBJECTS
1.
OF
RESEARCH
EXPERIMENTS
Definitions
Principal Investigator (PI)
The Principal Investigator is the staff member responsible for the research
project. In the case of student research, the supervisor assumes this
responsibility.
Student Investigator
The undergraduate or postgraduate student who will be collecting the
research data is referred to as the student investigator.
Student Research and Course-based Activities for Student Learning
Student research (undergraduate and postgraduate) refers to undergraduate
theses, supervised pilot research for an undergraduate or postgraduate
thesis, and research that is course-based. Course-based research activities
may include:
a. students conduct research interviews, administer research tests, or
distribute research questionnaires to develop interview of questionnaire
design skills
b. "mini" research projects where students pose research questions, gather
data from human participants, and analyze the data for presentation
c. other activities that would be considered research within the disciplinary
traditions in which the course is being taught.
2.
Licence
2.1
The Animals (Control of Experiments) Ordinance, Cap. 340, of the HKSAR
(referred to below as the Ordinance) provides for the control of experiments
on living vertebrate animals. No person except a licensee shall perform any
experiment involving living vertebrate animals, where a licence is applicable
and required under the Ordinance. For this purpose, Section 2 of the
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Ordinance stipulates that “experiment means any experiment performed on
an animal and calculated to give pain”.
2.2
2.3
A licence specifies the place(s) where the experiment may be conducted, its
purpose, duration and conditions where appropriate. In addition, special
endorsements and permit are required for performing experiments
(i)
for the purpose of attaining manual skill (Section 4 of the
Ordinance);
(ii)
for the purpose of illustrating lectures (Section 5);
(iii)
without administering any anaesthetic for killing the animal
(Sections 6 & 10).
PIs are requested to refer to Cap340 of the Ordinance available on the
Government
website
at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/AA6657F81C8EF4
98482575EE006DEC7E?OpenDocument for other details. In case of doubt on
whether a licence is required, advice of the licensing Authority should be
sought. Contact details of the Department of Health are as follows:
Address:
Tel:
Fax:
Email:
Special Health Services
Department of Health,
Room 79, 21/F, Wu Chung House
213 Queen’s Road East
Wan Chai
Hong Kong
(Re: Application for Licence under the Animals (Control of
Experiments) Ordinance)
2961 8645 / 2961 8978
2127 7329
ro_al@dh.gov.hk
Application forms for licences can be downloaded from the website of the
Department of Health:
http://www.dh.gov.hk/english/useful/useful_forms/useful_forms_ani.html.
3.
Conduct of Experiments
(i)
Experiments shall be conducted by appropriately qualified or trained
personnel in such a way as to avoid any unnecessary suffering or injury to the
animal.
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4.
(ii)
The scientist in charge of the experiment shall allow it to be terminated if its
continuation may result in unnecessary suffering or injury to the animal, and
shall ensure that authority to do so is delegated at times when he or she is
unavailable.
(iii)
If the experiment or procedure is likely to cause pain and discomfort, the
animals shall first be rendered incapable of suffering pain, by anaesthetization,
and be maintained in that condition until the experiment or procedure is
ended. The only exception to this guideline is where the anaesthetization
would defeat the purpose of the experiment and data cannot be obtained by
any other procedure. Such experiments or procedures shall be carefully
supervised by the Principal Investigator (and must be covered by a special
endorsement to a licence - see (a) above).
(iv)
Post-experiment care of animals shall be such as to minimize discomfort and
the consequences of any disability resulting from the experiment.
(v)
If it is necessary to kill an experimental animal, the animal shall be killed in a
humane manner; ie in such a way as to ensure immediate death. No animal
shall be discarded until after it is dead.
Government Legislation and Codes of Practice
4. 1
Government Legislation

Animals (Control of Experiments) Ordinance (Cap. 340) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/AA6657F81C8EF4
98482575EE006DEC7E?OpenDocument

Prevention of Cruelty to Animals Ordinance (Cap. 169) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/D2F86B91D0459F
CF482575EE0049049A?OpenDocument
4.2
Other related Government Legislation:

Dangerous Drugs Ordinance (Cap. 134) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/3E899AC9660B2A
CE482575EE00430767?OpenDocument

Antibiotics Ordinance (Cap. 137) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/8CA8AB37ACC51
404482575EE00437AB8?OpenDocument
-8-

Pharmacy and Poisons Ordinance (Cap. 138) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/0F66D4839C78DB
DA482575EE00438DD5?OpenDocument

Public Health (Animals and Birds) Ordinance (Cap. 139) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/194DC4B24628B1
7C482575EE0043DAB8?OpenDocument

Animals and Plants (Protection of Endangered Species) Ordinance, Cap. 187
at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/1460629FC781762
D482575EE004ACBFC?OpenDocument

Radiation Ordinance (Cap. 303) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/0209834806B62668
482575EE005B5796?OpenDocument

Occupational Safety and Health Ordinance (Cap. 509) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/9198BE222266C42
1482575EF0012128E?OpenDocument
For more information, please refer to http://www.legislation.gov.hk
4.3
Code of Practice

Code of Practice for Care and Use of Animals for Experimental Purposes
(2004) (by Agriculture, Fisheries & Conservation Department) at http://11718-78173.sunnyvisiondatacenter.com/english/quarantine/qua_awc/qua_awc_aw/q
ua_am_aw_code/qua_am_aw_code.html
Ethical-Animal (guideline)
(October 2013)
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Appendix II
CITY UNIVERSITY OF HONG KONG
Research Committee
APPLICATION FOR ETHICAL REVIEW OF
RESEARCH EXPERIMENTS INVOLVING ANIMAL SUBJECTS
IMPORTANT NOTES:
Please read carefully the following guidelines, government legislation and code of
practice BEFORE completing this form. Such references are also available in RO web
at http://www.cityu.edu.hk/ro/studentlan/dlAnimal.htm. All sections of the
application form must be completed. Please enter “Not applicable” if a section is
not relevant.
1) Guidelines on Ethical Review of Experiments Involving Animal Subjects as
attached
2) Government Legislation
i.
Animals (Control of Experiments) Ordinance (Cap. 340) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/AA6657F81C
8EF498482575EE006DEC7E?OpenDocument
ii.
Prevention of Cruelty to Animals Ordinance (Cap. 169) at
http://www.legislation.gov.hk/blis_pdf.nsf/CurAllEngDoc/D2F86B91D0
459FCF482575EE0049049A?OpenDocument
iii.
Other related government legislation, please refer to Section 4.2 of the
attached Guideline on Ethical Review of Research Involving Animals
Subjects for information.
3) Code of Practice for Care and Use of Animals for Experimental Purposes
(2004) at http://117-18-78173.sunnyvisiondatacenter.com/english/quarantine/qua_awc/qua_awc_aw/qu
a_am_aw_code/qua_am_aw_code.html
This form must be typewritten.
- 10 -
Part A: Basic Information
1) Title of Research Project
2) Principal Investigator* / staff in charge of the research project:
Name
Dept/Unit
Post
Tel. No.
Email
Student Investigator/Department (applicable to student project only)
Name
*
Dept/Unit
Programme
Email
In the case of student research, the supervisor assumes this responsibility.
3) Type of Project
Grant Type, if any (for staff research)
Other (please specify)
Funding Agency (if applicable)
Thesis Research
PhD
MPhil
Bachelor’s
Degree
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Part B: Proposal/Project Details and Methodology (Please complete all sections)
1) Names of Staff/Students Involved in Experiments
(a) Name
Dept/Unit
Post
Tel. No.
Email
(b) Brief description of experience (including types/duration of training attended) of the
persons names in Part B 1(a) with regard to animal experimentation:
2) Similarity to previously approved protocol
(a) whether the substantive content of this protocol is similar to that of a previously
approved protocol (please tick):
Yes, please state the number of previous projects that use similar protocols:
_____________________________
No
(b) If yes, please provide details of the latest similar project :
(i)
Project title:
(ii)
Reference no:
(iii)
Date of Approval:
(iv)
Capacity : PI / Co-I / Co-PI / Others (please specify) : ______________________
(Please attach animal ethics approval document if available)
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3) Type of experiment for the currently submitted protocol (please tick)
(a) Acute or Chronic Experiment (please tick one)
Acute (involving euthanasia of animals only or procedures to be performed on
animals under anesthesia followed by euthanasia or procedures to be performed
on conscious animals followed by euthanasia; all procedures to be completed
within 24 hours)
Chronic (involving long-terms [i.e. more than 24 hours] holding animals;
procedures to be performed on conscious animals with or without anaesthesia)
(b) Non-invasive or Invasive Experiment (please tick one)
Non-invasive
Invasive (involving puncture or incision of the skin or insertion of an instrument
or instrument or foreign material into the body of animals)
4) Types of Licence/Permit/Endorsement Required
(Please refer to Section 2 of the Guidelines on Ethical Review of Research Involving
Animal Subjects and Cap. 340 for information. Applicants are reminded that
licence/permit/endorsement should be obtained from the Department of Health before the
commencement of the experiments. Every licensee is required under this Ordinance to
submit an annual return to the government on or before the first day of January each year.
Please
refer
to
the
website
of
the
Department
of
Health
at
http://www.dh.gov.hk/english/useful/useful_forms/useful_forms_ani.html for application
details. )
a) Type of Licence/Permit/Endorsement Required (please check the box(es) as
appropriate)
i) Licence to Conduct Experiment alone
ii) Endorsement to Authorize Experiments to Attain Manual Skill
iii) Teaching Permit (for illustrating lectures)
iv) Endorsement to Authorize Experiments without Anaesthetics or without
Destroying the Animal
b) Application Status of the Licence/Permit/Endorsement (please check the box as
appropriate)
i) Valid Licence/Permit/Endorsement Approved
ii) Application Submitted and being Considered
iii) Application NOT yet Submitted (please state the proposed schedule below)
_________________________
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c) If approval is required by other authorities, please complete this section.
i) Please indicate if the experiments on live animals are to be carried out in
another institution
Yes
No
If yes, please specify the name of the institution: _______________________
ii) Ethical approval involving animals from the institution has been sought:
Yes
Approval being sought
Note: Principal Investigators have to ensure that approval has already been
sought from ethics committee of the host institution before commencement of
the experiments involving animals.
5) Explanation of how the project advances scientific knowledge and confirmation that
alternatives to experiments on live animals have been considered but are not appropriate
and/or a reduction of the number of animals to be used have been considered and why
such alternatives/reductions are not possible (please attach separate A-4 size sheets if
necessary):
6) Species/Strain and Number of Animals to be Used
Species/Strain:
No. of animals to be used at CityU:
No of animals to be used at Collaborating Institute:
(only apply if the experiment(s) is/are to be conducted at Collaborating Institute)
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7) Proposed Location where Animals are to be Kept
8) Place in Which Experiment(s) will be Carried Out
9) The Likely Duration of the Experiments e.g. number of session per year, duration per
session.
10) Outline of procedures, with special reference to procedures likely to cause suffering or
injury. Experiments should be conducted in such a way as to avoid any unnecessary
suffering and injury to the animals. [Please indicate if the protocol being used in this project
is the same as the one stated under Part B 2(b). If not, please highlight the differences.]
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11) Measures to be taken to minimize suffering/injury, including details of anaesthetist,
analgesics, euthanasia etc to be used (please specify the relevant guidelines if any, that will
be followed, for example, AVMA Guidelines on Euthanasia.
12) Post-experiment Care of Animals
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Declaration (This section must be completed.)
I understand that all experiments involving the use of living vertebrate animals must fulfill
all relevant legislation and code of practice in Hong Kong and I have read carefully the
following guidelines, government legislation and code of practice relevant to the use of
animal subjects in experiments including, but not limited to, the following:
(please indicate and declare by checking the following boxes.)
Guidelines on Ethical Review of Experiments involving Animal Subjects
Animals (Control of Experiments) Ordinance (Cap. 340)
Prevention of Cruelty to Animals Ordinance (Cap. 169)
Other related Ordinances as stated in the Section 4.2 of the Guidelines on Ethical
Review of Research Involving Animal Subjects
Code of Practice for Care and Use of Animals for Experimental Purposes
I certify that I am familiar with and will comply with all rules and legal requirements and
pertinent institutional rules/policies on the use of living animals in experiment(s). I shall
undertake to exercise reasonable care to ensure that the proposed experiment(s) is conducted
in a manner that is consistent with these standards of ethical practice. I understand that
failure to observe the University's published protocol may constitute malpractice and be a
ground for disciplinary action by the University.
Signature
(Principal Investigator/Staff/Supervisor)
Date
To be Completed by Head of Department
I endorse this application on the basis of the information provided and the declaration of the
PI/Supervisor.
Signature
(Head of Department)
Date
Appendix III
ANIMALS (CONTROL OF EXPERIMENTS) REGULATIONS
FORM 1
Application Form
To : The Director of Health
I,
of
on the grounds hereinafter mentioned, hereby apply for —
(a) a Licence under section 7 of the Animals (Control of Experiments) Ordinance
(Cap. 340).
(b) an endorsement / thereto / *to my existing Licence No.
*
dated
/ under section 8 of the said Ordinance.
(c) a teaching permit under section 9 of the said Ordinance.
(d) an endorsement to / the said Licence / *my existing Licence No.
dated
/ under section 10 of the said Ordinance.
Grounds for application.
Type of experiment(s).
Purpose of experiment(s).
Place where experiment(s) may be conducted.
Qualifications of Applicant and any posts held.
Dated
Signed
*Delete as appropriate.
Annex
The section below is not part of Form 1 but information supplied would help avoid unnecessary delay
in processing your application.
Please put a ‘tick’ to the box against each of the followings which are applicable to your
application.
1. □ I have not been granted a licence for the experiment under application before; [please go
to (2) and (3a or 3b) only] OR
□ I am the holder of a valid licence for the experiment under application; (Reference number
of licence: ________________ ) [please go to (2) and (4) only] OR
□ I am not the holder of a valid licence for the experiment under application but I have been
previously granted a licence for the experiment under application which has now expired.
(Reference number of latest licence: _______________) [please go to (2) and (5) only]
2. □ I hereby declare that in accordance with Regulations 4 and 5 of the Animals (Control of
Experiments) Regulations, Cap.340A (“the Regulations”), I shall keep up-to-date a book
in the form set out as Form 6 in the Schedule to the Regulations and I shall render to the
Director of Health on or before the 1st day of January each year a return in the form set
out as Form 7 in the Schedule to the Regulations of all experiments* performed by me
during the preceding twelve months.
3. (a)
□
□
□
□
Application for a licence without an “Endorsement to Enable Performance of
Experiments without Anaesthetics or without Destroying the Animal” under section
10 of the said Ordinance –
I confirm that throughout the whole of the experiment the animal is under the influence of
some anaesthetic of sufficient power to prevent the animal feeling pain; and if the pain is
likely to continue after the effect of the anaesthetic has ceased, or if any serious injury has
been inflicted on the animal, the animal is killed before it recovers from the influence of the
anaesthetic which has been administered; AND
I confirm that conditions/ well-being of the animals will be monitored during the
experiment; AND
I confirm that animals with signs of severe distress or pain will be euthanized before the
end of the study; AND
I confirm that the following method(s) to be used for sacrificing the animals will not cause
unnecessary/ prolonged pain to them:
□ cervical dislocation ( □ under anaesthesia OR □ not under anaesthesia )
□ decapitation ( □ under anaesthesia OR □ not under anaesthesia )
□ overdose of anaesthetic
□ carbon dioxide asphyxiation
□ exsanguinations under anaesthesia
□ other(s), please specify:________________________________________
(b) Application for a licence with an “Endorsement to Enable Performance of
Experiments without Anaesthetics or without Destroying the Animal” under section
10 of the said Ordinance –
□ I confirm that the experiment would necessarily be frustrated by –
□ the performance of such experiment under any anaesthetic; AND/OR
□ killing the animal on which such experiment is performed before it recovers from the
influence of any anaesthetic.
Please indicate why –
4.
□
5.
□
I confirm that the experimental procedures under the type of experiment(s)* and the
purpose of experiment(s)* are exactly the same as that of my existing licence under the
reference number quoted above; AND
□ I will not conduct any experiment* after the expiry date of my existing licence under the
reference number quoted above until a new licence is issued AND I have been keeping a
proper Form 6 in accordance with regulation 4 of the Regulations.
I confirm that the experimental procedures under the type of experiment(s)* and the
purpose of experiment(s)* are exactly the same as that of my previous licence under the
reference number quoted above; AND
□ I hereby declare that I have not conducted any experiment* after the expiry date of my
previous licence under the reference number quoted above AND I have kept a proper Form
6 during the validity period of my previous licence under the reference number quoted
above in accordance with regulation 4 of the Regulations.
“experiment” means any experiment performed on a living vertebrate animal and calculated to give pain
(section 2 of the Animals (Control of Experiments) Ordinance, Cap. 340).
Full name**:
HK Identity Card/Passport/ Travel Document No.:
Email Address:
Contact No.:
Mobile No.:
(Facsimile)
Signed
(Institute/Company chop)***
(Applicant)
** Full name as appears on HK Identity Card/Passport/Travel Document
*** Please obtain an official chop of the Institute/ Company where you are working or studying.
Appendix IV
Regulations for Personnel Working in Animal Holding Room (at Nam Shan Entrance)
1. Access to the animal facility is only limited to authorized persons.
2. All users should wear laboratory gowns and anti-bite gloves when handling animals.
3. Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing
food for human use inside animal holding facility are forbidden. Persons who wear
contact lenses should also wear goggles or face shield when working with animals.
4. Door to animal holding room should be kept closed when experimental animals are
present.
5. All animal-holding cages must be closed properly.
6. All procedures should be carefully performed to minimize the generation of aerosols.
7. Work surfaces should be decontaminated after use or after any spill of viable
materials.
8. Carcasses and used bedding must be properly wrapped up and kept in the
designated freezer until collected by licensed clinical waste collector.
9. Personnel should wash their hands after handling animals.
10. Any injury and accident must be reported to the technical staff in charge.
Appendix V
Regulations for Personnel Working in Rodent Dissection Area (P1702)
1. No housing of rodent is allowed in Rodent Dissection Area. The room and facilities
inside are restricted to rodent-associated experiments only.
2. All users should wear laboratory gowns and anti-bite gloves when handling
animals.
3. Laboratory gowns or uniforms worn in the Rodent Dissection Area should not be
worn in other areas.
4. Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing
food for human use inside Rodent Dissection Area are forbidden. Persons who
wear contact lenses should also wear goggles or face shield when working with
animals.
5. Door to Rodent Dissection Area should be kept closed when experimental animals
are present. The sign ‘Rodent-associated experiment in progress’ should be posted
up when there are animal inside Rodent Dissection Area.
6. Cultures, tissues or specimens of body fluids should be placed in leak-free
containers during collection, handling, processing, storage and transportation.
7. Broken glassware must not be handled directly by hand, but must be removed by
mechanical means such as brushes and dustpans, tongs or forceps.
8. Used needles, sharps and broken glassware should be disposed into designated
sharp boxes.
9. Carcasses, isolated animal tissues and wastes must be properly wrapped up and
kept in the designated freezer until collected by licensed clinical waste collector.
10. Work surfaces should be decontaminated after use and after any spill of viable
materials.
11. Personnel should wash their hands after handling animals and before leaving the
Rodent Dissection Area.
12. Any injury and accident must be reported to the technical staff in charge.