SDSU Institutional Animal Care and Use Committee
Animal Protocol Form (APF)
Animal Breeding, Transgenic or other Genetically Modified Animals
Supplement 2
For Official Use Only
APF #:
Submission Date:
Approval Date:
PI Name:
I.
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Breeding
The use of genetically defined rodents is a powerful research tool that often requires breeding
of the animals as opposed to simply ordering all of the animals to be used from some outside
source. AT SDSU, breeding of animals for experimental purposes is limited to rodent species.
Investigators are encouraged to utilize the services of the SDSU Office of Laboratory Animal
Care (OLAC) and the Mouse Genomics Core to assist in rodent breeding and maintenance.
Note: Ensure that any laboratory personnel responsible for the breeding program are listed in
the Main APF Personnel Section A.
1. Strains / Lines/ Stock
Please complete the following table for each line or strain to be bred. For each strain
indicate the estimated number of animals for breeding and experimental use, the estimated
number and disposition of offspring and whether the breeding management will be
overseen by OLAC or by another group such as laboratory personnel.
Strain/Stock
/Line
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Estimated # of
Breeders
Breeding Research
Only
Use
Estimated # of Offspring
Total Number
Breeding
Only
Culled
Research
Use
NonResearch
research Use
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Total
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Breeding
Management
OLAC Other
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Version June 15,2015
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SDSU Institutional Animal Care and Use Committee
Animal Protocol Form (APF)
Animal Breeding, Transgenic or other Genetically Modified Animals
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2. Breeding Colony Maintenance
a. For each line, describe how the numbers needed for this APF were determined.
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b. Describe endpoints or interventions related to complications pertaining to breeding
such as aggression, cannibalism, neonatal trauma, orphan pups, or dystocia.
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c. Relative to the breeding of the animals, describe any known or potential
complications related to the particular strain(s) such as poor breeding performance,
low birth weights, low birth number, neonatal mortality, delayed weaning,
shortened breeding life-span, etc. and how the complications will be managed.
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d. By checking the following boxes, as the PI on this APF I have read and agree to abide
by the following policies, SOPs, guidelines and recommendations:
☐ SDSU Breeding and Cage Density IACUC Policy
☐ SDSU Breeding and Cage Density OLAC SOP
☐ I would like to deviate from the above listed Breeding and Cage Density IACUC Policy or
OLAC SOP as described below:
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e. Provide the name(s) of personnel who will be responsible for the breeding
maintenance or indicate “OLAC Personnel.” Also include the personnel information
in Section A PERSONNEL in the Main APF.
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II.
Transgenic or Other Genetically Modified Strains
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Version June 15,2015
SDSU Institutional Animal Care and Use Committee
Animal Protocol Form (APF)
Animal Breeding, Transgenic or other Genetically Modified Animals
Supplement 2
1. Will any of the animals be created or genetically manipulated utilizing the Mouse
Genomics Core (MGC)?
☐ Yes. Complete the Genetically Modified Mouse Order Form and submit to
iacuc@mail.sdsu.edu.
☐ No. Animals will be obtained from an approved vendor or received from another
institution following all SDSU rodent quarantine requirements. Contact the
Office of Laboratory Animal Care (OLAC) (619) 594-5421 for more information.
☐ No. Animals will be genetically manipulated as part of this protocol submission.
2. With regard to the generation of new lines of transgenic/genetically modified animals
as part of this protocol submission please check any/all that apply:
☐ DNA microinjection
☐ Embryonic stem cell-mediated gene transfer
☐ Retrovirus-mediated gene transfer
 Chemical Means (ex. N-ethyl-N-nitrosourea ENU)
☐ Other Explain:
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3. Please describe each method of generating transgenic/genetically modified animals in
detail including any agents administered. Note: Please complete Supplement 4 for any
substances (hormones, biological materials, chemicals) administered and Supplement 6
for any surgical interventions.
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4. Please provide the justification for the generation of genetically modified rodents.
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5.
Relative to the genetic manipulation, describe any known or potential adverse effects
that could be associated with the desired genotype such as physical impairments,
effects on breeding performance, life-span, and overall health and wellbeing of the
animal.
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6. Describe any special care or monitoring the animals will require including what
measures will be undertaken to alleviate any pain and/or distress. Include criteria for
euthanasia.
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III.
Tissue for Genetic Analysis
1. Will tissue be collected for genetic analysis?
☐ No
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Version June 15,2015
SDSU Institutional Animal Care and Use Committee
Animal Protocol Form (APF)
Animal Breeding, Transgenic or other Genetically Modified Animals
Supplement 2
☐ Yes
If yes, please describe whether the tissues will be collected postmortem or antemortem,
the method of tissue collection including type of tissue, amount of tissue, methods to
alleviate pain and distress, age of animals at time of collection, species and monitoring of
animals. List type(s) of restraint used in Section G3 of the Main APF.
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Version June 15,2015