Fig. S1. EPR spectrum of LccA purified from H. volcanii US02 (—, solid line) with simulation (‒ ‒,
broken line). Simulation parameters are listed in the figure. The spectroscopic parameters were ν =
9.442357 GHz, Pmw = 0.63 mW, 10 G mod. amp., 60 dB receiver gain, 41 ms time constant, 82 ms
sampling time, 1.563 G/point, 16 averages, T = 20 K. The spectrum was normalized and baseline
corrected against a background of buffer solution. Simulations were carried out with the easyspin
toolbox in Matlab™. g┴, g║, and a║ were freely varied parameters while all other parameters were
judiciously chosen prior to the simulation.
Fig. S2. EPR spectrum of LccA from E. coli US05 (—, solid line) with simulation (‒ ‒, broken line).
Simulation parameters are listed in the figure. The spectroscopic parameters were ν = 9.443192 GHz,
Pmw = 0.63 mW, 10 G mod. amp., 60 dB receiver gain, 41 ms time constant, 82 ms sampling time, 1.563
G/point, 4 averages, T = 20 K. The spectrum was normalized and baseline corrected against a
background of buffer solution. Simulations were carried out with the easyspin toolbox in Matlab™. g┴,
g║, and a║ were freely varied parameters while all other parameters were judiciously chosen prior to
the simulation.
Fig. S3. EPR spectrum of 100 μM Cu(II) in buffer solution (—, solid line) with simulation (‒ ‒, broken
line). Simulation parameters are listed in the figure. The spectroscopic parameters were ν = 9.442082
GHz, Pmw = 0.63 mW, 10 G mod. amp., 60 dB receiver gain, 41 ms time constant, 82 ms sampling time,
1.563 G/point, 16 averages, T = 20 K. The spectrum was normalized and baseline corrected against a
background of buffer solution. Simulations were carried out with the easyspin toolbox in Matlab™. g┴,
g║, and a║ were freely varied parameters while all other parameters were judiciously chosen prior to
the simulation.
Fig. S4. EPR spectrum of the superdex fraction of LccA purified from H. volcanii US02 (—, solid line)
with type I (‒·‒, modified line 1) and type II (‒ ‒, broken line) Cu(II) simulations in the approximate
relative intensities necessary to reproduce the experimental spectrum. In addition, the sum of both
simulations is shown (‒ ‒ ·, modified line 2). The parameters for type I and type II Cu(II) were obtained
from the individual simulations shown in figs S.1 and S.2.
Fig. S5. Same spectra as in fig. S.4 plus single and double integrals of the simulations. The double
integrals show the approximate relative intensities of the contributions of type I and type II Cu to the
experimental spectrum of LccA purified from H. volcanii US02 (relative intensities of 251 and 115,
respectively).