Shin’ichi ISHIWATA, PhD (D.Sci.)
E-mail: ishiwata@waseda.jp
URL: http://www.phys.waseda.ac.jp/bio/ishiwata/index.html
Affiliation:
Professor, Department of Physics, Faculty of Science and Engineering, Waseda University
Director, Waseda Bioscience Research Institute in Singapore (WABIOS)
Academic Background:
1975
Doctor of Science, Dept. of Physics, Faculty of Science, Nagoya University
1971
Master of Science, Dept. Physics, Faculty of Science, Nagoya University
1969
Bachelor of Science, Dept. Physics, Faculty of Science, University of Tokyo
Professional Carrier:
2006Professor in Department of Physics, Faculty of Science and Engineering Waseda Universit y
1986-2006 Professor in Department of Physics, School of Science and Engineering, Waseda University
1981-1986 Associate Professor in Department of Physics, School of Science and Engineering,
Waseda University
1979-1981 Lecturer in Department of Physics, School of Science and Engineering, Waseda University
1978-1979 Boston Biomedical Research Institute, Post-doctoral Fellow
1976-1977 Post-doctoral Fellow of Massachusetts Institute of Technology
(Post-doctoral Fellow of Muscular Dystrophy Association of America)
1974-1975 (Pre- and) Post-doctoral Fellow of Japan Promotion of Science
Society Post:
The President of The Biophysical Society of Japan (BSJ) (2004.1 -2005.12), The Vice-President of BSJ
(1996.1-1997.12), Editor-in-Chief of Biophysics (Japanese) (2000.1-2001.12), Editor-in-Chief of
BIOPHYSICS (English, On-line Journal of BSJ) (2008.4-present), Program Officer of JSPS (2007.42010.3), Editorial Board member of Biophys. J. (USA) (2006-2012), and EPJE (2012-), Member of
Committee of Scientist in Human Frontier Science Program Organization (2013 -) etc.
Society membership:
Biophysical Society of Japan, Physical Society of Japan, Biochemical Society of Japan, Physiological
Society of Japan, Biophysical Society of USA.
Major publications :
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Takagi, J., Itabashi, T., Suzuki, K., Kapoor, T. M., Shimamoto, Y. and Ishiwata, S. (2013) Cell Rep. 5, 44-50,
"Using micromanipulation to analyze control of vertebrate meiotic spindle size"
Sato, K., Kuramoto, Y., Ohtaki, M., Shimamoto, Y. and Ishiwata, S. (2013). Phys. Rev. Lett. 111, 108104.
"Locally and globally coupled oscillators in muscle"
Itabashi, T., Terada, Y., Kuwana, K., Kan, T., Shimoyama, I. and Ishiwata, S. (2012) . Proc. Natl. Acad. Sci.
U.S.A. 109, 7320-7325. "Mechanical impulses can control metaphase progression in a mammalian cell."
Shimamoto, Y., Maeda, Y.T., Ishiwata, S., Libchaber, A.J. and Kapoor, T.M. (2011). Cell 145, 1062-1074.
"Insights into the micro-mechanical properties of the metaphase spindle."
Oguchi, Y., Uchiyama, S., Ohki, T., Mikhailenko, S.V., and Ishiwata, S. (2011). Nature Cell Biol. 13, 846-852.
"The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends."
Ishiwata, S., Shimamoto, Y., and Fukuda, N. (2011). Prog. Biophys. Mol. Biol. 105, 187-198. "Contractile
system of muscle as an auto-oscillator."
Sato, K., Ohtaki, M., Shimamoto, Y., and Ishiwata, S. (2011). Prog. Biophys. Mol. Biol. 105, 199-207. "A
theory on auto-oscillation and contraction in striated muscle."
Oguchi, Y., Mikhailenko, S.V., Ohki, T., Olivares, A.O., De La Cruz, E.M., and Ishiwata, S . (2010). Nature
Chem. Biol. 6, 300-305. "Robust processivity of myosin V under off-axis loads."
Shimamoto, Y., Suzuki, M., Mikhailenko, S.V., Yasuda, K. and Ishiwata, S. (2009). Proc. Natl. Acad. Sci.
U.S.A. 106, 11954-9. “Inter-sarcomere coordination in muscle revealed through individual sarcomere response
to quick stretch.”
Itabashi,T., Takagi,J., Shimamoto,Y., Onoe,H., Kuwana,K., Shimoyama,I., Gaetz,J., Kapoor, T.M. and Ishiwata,
S. (2009). Nature Meth. 6, 167-172. “Probing the mechanical architecture of the vertebrate meiotic spindle.”
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Oguchi, Y. Mikhailenko, S. V., Ohki, T., Olivares, A. O., De La Cruz, E. M. and Ishiwata, S. (2008). Proc. Natl.
Acad. Sci. U.S.A. 105, 7714-7719. "Load-dependent ADP binding to myosins V and VI: Implications for
subunit coordination and function."
Suzuki, M., Tseeb, V., Oyama, K. and Ishiwata, S. (2007). Biophys. J. 92, L46-L48. “Microscopic detection of
thermogenesis in a single HeLa cell.”
Suzuki, M., Fujita, H. and Ishiwata, S. (2005). Biophys. J. 89, 321-328. "A new muscle contractile system
composed of a thick filament lattice and a single actin filament."
Uemura, S., Higuchi, H., Olivares, A., De La Cruz, E. M. and Ishiwata, S. (2004). Nature Struct. Mol. Biol. 11,
877-883. “Mechanochemical coupling of two substeps in a single myosin V motor.”
Uemura, S. and Ishiwata, S. (2003). Nature Struct. Biol. 10, 308-311. “Loading direction regulates the affinity
of ADP for kinesin.”
Uemura,S., Kawaguchi,K., Yajima,J., Edamatsu,M., Toyoshima,Y.Y. and Ishiwata,S. (2002).Proc.Natl.Acad.Sci.
U.S.A. 99, 5977-5981. “Kinesin-microtubule binding depends on both nucleotide state and loading direction.”
Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T. and Ishiwata, S. (2002). Nature Cell Biol. 4, 666-673.
“Microscopic analysis of polymerization dynamics with individual actin filaments.”
Ali, M. Y., Uemura, S., Adachi, K., Itoh, H., Kinosita, Jr., K. and Ishiwata, S. (2002). Nature Struct. Biol. 9,
464-467. “Myosin V is a left-handed spiral motor on the right-handed actin helix.”
Kawaguchi, K. and Ishiwata, S. (2001). Science, 291, 667-669. “Nucleotide-dependent single- to double-headed
binding of kinesin.”
Kato, H., Nishizaka, T., Iga, T., Kinosita, Jr., K. and Ishiwata, S. (1999). Proc. Natl. Acad. Sci. U.S.A. 96,
9602-9606. "Imaging of thermal activation of actomyosin motors."
Fujita, H., Yasuda, K., Niitsu, S., Funatsu, T. and Ishiwata, S. (1996). Biophys. J. 71, 2307-2318. "Structural
and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle."
Nishizaka, T., Miyata, H., Yoshikawa, H., Ishiwata, S. and Kinosita, Jr., K. (1995). Nature 377, 251-254.
"Unbinding force of a single motor molecule of muscle measured using optical tweezers."
Nishizaka, T., Yagi, T., Tanaka, Y. & Ishiwata, S. (1993). Nature 361, 269-271. "Right-handed rotation of an
actin filament in an in vitro motile system."
Funatsu, T., Higuchi, H. and Ishiwata, S. (1990). J. Cell Biol. 110, 53-62. "Elastic filaments in skeletal muscle
revealed by selective removal of thin filaments with plasma gelsolin."
Ishiwata, S., Kinosita, Jr. K., Yoshimura, H. and Ikegami, A. (1987). J. Biol. Chem. 262, 8314-8317.
"Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements."
Ishiwata, S. and Funatsu, T. (1985). J. Cell Biol. 100, 282-291. "Does actin bind to the ends of thin filaments in
skeletal muscle?"
Thomas, D. D., Ishiwata, S., Seidel, J. C. and Gergely, J. (1980). Biophys. J. 48, 873-890. "Submillisecond
rotational dynamics of spin-labeled myosin heads in myofibrils."
Tanaka, T., Ishiwata, S. and Ishimoto, C. (1977). Phys. Rev. Lett. 38, 771-774. "Critical behavior of density
fluctuations in gels."
Kondo, H. and Ishiwata, S. (1976). J. Biochem. 79, 159-171. "Uni-directional growth of F-actin."
Oosawa, F., Fujime, S., Ishiwata, S. and Mihashi, K. (1972). Cold Spr. Harb. Symp. Quant. Biol. 37, 277-285.
"Dynamic property of F-actin and thin filament."
Ishiwata, S. and Fujime, S. (1972). J. Mol. Biol. 68, 511-522. "Effect of calcium ions on the flexibility of
reconstituted thin filaments of muscle studied by quasielastic scattering of laser light."
Fujime, S. and Ishiwata, S. (1971). J. Mol. Biol. 62, 251-265. "Dynamic study of F-actin by quasielastic
scattering of laser light."
Current research interests:
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Single-molecular mechanism of protein motors such as myosin II, V & VI and kinesin s
Molecular mechanism and physiological significance of SPOC (Spontaneous Oscillatory Contraction) of skeletal
and cardiac muscles
Structural and functional reconstruction, functional analysis and formation mechanism of cytoskeleton and the
contractile system of muscle
Micro-mechanics of meiotic spindle, mechanical control of check point for chromosome segregation in cells
Application of temperature pulse technique to cells, and imaging of temperature distribution of single cells