Supplement for ‘Risk factors for urinary schistosomiasis and active trachoma in Burkina Faso before preventive chemotherapy: implications for monitoring and evaluation of integrated control programmes’ Artemis Koukounari, Seydou Touré, Christl A. Donnelly, Amadou Ouedraogo, Bernadette Yoda, Cesaire Ky, Martin Kaboré, Elisa Bosqué-Oliva, María-Gloria Basáñez, Alan Fenwick, Joanne P. Webster S.1 Here we present two tables that contain Pearson correlations. The first table (i.e. Table S.1) contains correlations between the average values of weather and environmental variables as derived from the 9 weather stations. The second table (i.e. Table S.2) contains Pearson correlations between the school-level prevalences of single and dual infections (i.e. n=21) with the interpolated values of the weather and environmental variables through inverse distance weighting (i.e. assigned values to unknown points of weather and environmental variables for schools from the known average values as derived from the weather stations). Table S.1 Mean altitude Mean precipitation Mean max temp Mean min temp Mean temp av Mean 1 altitude Mean 0.410 1 precipitation p=0.273 Mean max 1 -0.711 -0.825 temp p=0.032 p=0.006 Mean min -0.435 0.650 1 -0.677 temp p=0.241 p=0.058 p=0.045 Mean av -0.359 1 -0.885 0.800 0.875 temp p=0.342 p=0.002 p=0.010 p=0.002 Mean air -0.639 -0.284 0.623 -0.047 0.096 pressure p=0.064 p=0.459 p=0.073 p=0.905 p=0.806 Bold typeface is used for the results statistically significant at the 0.05 significance level. Mean air pressure 1 1 Table S.2 Altitude◊ Precipitation◊ Max temperature◊ Min temperature◊ Av temperature◊ Air pressure◊ S. haematobium prevalence◙ Active trachoma prevalence◘ -0.595 p=0.005 -0.631 p=0.002 0.688 p<0.001 0.440 p=0.046 0.543 p=0.011 0.413 p=0.063 0.771 p<0.001 0.470 p=0.031 -0.693 p<0.001 -0.384 p=0.086 -0.399 p=0.073 -0.762 p<0.001 Prevalence of coinfections with S. haematobium and active trachoma□ -0.411 p=0.064 -0.436 p=0.048 0.424 p=0.055 0.274 p=0.230 0.342 p=0.129 0.331 p=0.143 Bold typeface is used for the results statistically significant at the 0.05 significance level. ◊ Interpolated values of environmental variables. ◙ S. haematobium prevalence at the school level (single infections with S. haematobium and co-infections with active trachoma are included in these calculations). ◘ Active trachoma prevalence at the school level (single infections with active trachoma and co-infections with S. haematobium are included in these calculations). □ Only co-infections with S. haematobium and active trachoma at the school level are included in these calculations. S.2 Scatter plots are displayed below to show values for the interpolated values of the environmental variables and the school-level prevalences of single and dual infections. Initially six scatter plots are displayed for the S. haematobium prevalences at the school level and the interpolated values of the environmental variables; then another six scatter plots are displayed between the active trachoma prevalences at the school level and the interpolated values of the environmental variables. Finally six scatter plots are displayed for the prevalences of co-infections with S. haematobium and active trachoma at the school level and the interpolated values of the environmental variables. 2 60 S. haematobium school prevalence S. haematobium school prevalence 60 50 40 30 20 10 0 0 100 200 300 400 50 40 30 20 10 0 500 0 0.5 1 Altitude (MSL) school prevalence 40 30 20 10 0 33 2.5 3 3.5 34 35 36 37 38 50 40 30 20 10 0 21.5 Max temperature (celsius) 22 22.5 23 23.5 Min temperature (celsius) 60 60 S. haematobium school prevalence school prevalence 2 60 50 S. haematobium S. haematobium school prevalence 60 S. haematobium 1.5 Precipitation (mm) 50 40 30 20 10 0 27 27.5 28 28.5 29 Av temperature (celsius) 29.5 30 50 40 30 20 10 0 960 965 970 975 980 Air pressure (millibar) Points at all the six scatter plots above show S. haematobium prevalence at the school level (single infections with S. haematobium and co-infections with active trachoma are included in these calculations) versus the interpolated values of the environmental variables. 3 40 Active trachoma school prevalence 40 Active trachoma school prevalence 35 30 25 20 15 10 5 0 0 100 200 300 400 35 30 25 20 15 10 5 0 0 500 0.5 1 2 2.5 3 3.5 40 Active trachoma school prevalence Active trachoma school prevalence 40 35 30 25 20 15 10 5 35 30 25 20 15 10 0 33 34 35 36 37 38 5 0 21.5 22 22.5 23 23.5 Min temperature (celsius) M ax temperature (celsius) 40 40 35 35 Active trachoma school prevalence Active trachoma school prevalence 1.5 Precipitation (mm) Altitude (MSL) 30 25 20 15 10 5 0 30 25 20 15 10 5 0 27 27.5 28 28.5 29 Av temperature (celsius) 29.5 30 960 965 970 975 980 Air pressure (millibar) Points at all the six scatter plots above show active trachoma prevalence at the school level (single infections with active trachoma and co-infections with S. haematobium are included in these calculations) versus the interpolated values of the environmental variables. 4 4.5 4 Co-infections school prevalence Co-infections school prevalence 4.5 3.5 3 2.5 2 1.5 1 0.5 4 3.5 3 2.5 2 1.5 1 0.5 0 0 0 100 200 300 400 0 500 0.5 1 2 2.5 3 3.5 4.5 4.5 4 Co-infections school prevalence Co-infections school prevalence 1.5 Precipitation (mm) Altitude (MSL) 3.5 3 2.5 2 1.5 1 0.5 4 3.5 3 2.5 2 1.5 1 0.5 0 21.5 0 33 34 35 36 37 22 38 22.5 23 23.5 Min temperature (celsius) Max temperature (celsius) 4.5 4 Co-infections school prevalence Co-infections school prevalence 4.5 3.5 3 2.5 2 1.5 1 0.5 0 27 27.5 28 28.5 29 Av temperature (celsius) 29.5 30 4 3.5 3 2.5 2 1.5 1 0.5 0 960 965 970 975 980 Air pressure (millibar) Points at all the six scatter plots above show prevalences of co-infections at the school level (single infections are not included in these calculations) versus the interpolated values of the environmental variables. 5 S.3 Scatter plots of predicted versus observed values were inspected in order to examine the appropriateness of the binomial and multinomial multivariate fitted models. Figure S.3.1 shows predicted versus observed S. haematobium prevalence as derived from the single pathogen binomial logistic regression model (results of which are indicated in Table 3 in the main text). Figure S.3.2 shows predicted versus observed active trachoma prevalence as derived from the single pathogen binomial logistic regression model (results of which are indicated in Table 4 in the main text). Figure S.3.3 shows predicted versus coinfections prevalence by assuming independence and multiplying together the predictions from these two single pathogen binomial logistic regression models. This figure suggests that the individual models may overestimate co-infections and therefore a multinomial logistic regression model was used in order to quantify risk factors for single and coinfections (results of which are indicated in Table 5 in the main text). Figure S.3. 4 shows predicted versus observed co-infections as derived from the multinomial logistic regression model. Figure S. 3. 1 From binary logistic S. haematobium regression model (Table 3 in paper) 50 40 Observed S. haematobium prevalence 30 20 10 0 0 10 20 30 40 50 Predicted S. haematobium prevalence 6 Figure S. 3. 2 From binary logistic active trachoma regression model (Table 4 in paper) Observed active trachoma prevalence 40 35 30 25 20 15 10 5 0 0 10 20 30 40 Predicted active trachoma prevalence Figure S. 3. 3 Observed co-infections Multiplication of predictions of co-infections from the two single pathogens logistic regression models. 8 7 6 5 4 3 2 1 0 0.00 2.00 4.00 6.00 Predicted co-infections 8.00 7 Figure S. 3. 4 From mutlinomial logistic regression model (Table 5 in paper) Observed coinfections 8 6 4 2 0 0 2 4 6 8 Pre dicte d co-infe ctions 8