The Society for Biotechnology, Japan Journal of Bioscience and Bioengineering Checklist January 2016 Please read all items carefully and check applicable boxes to indicate that you have understood and agreed with the statements. Manuscripts that do not meet the journal’s requirements will be returned to the author for modification. Instructions to authors are available at http://www.sbj.or.jp/e/jbb/jbb_instructions.html. Guidance on ethical standards for authors (which JBB expects authors to follow) are available at http://publicationethics.org/files/International%20standards_authors_for%20website_11_Nov_2011.pdf. Title: please print Authors: please print AUTHORSHIP All authors agree to take accountability for all aspects of the work. Multiple corresponding authors are not allowed. The corresponding (submitting) author vouches that: he/she will be responsible for all communications between the journal and the authors he/she will provide correct current contact details for all coauthors all authors have approved the submitted version of the manuscript all authors have agreed to submission to JBB all authors meet the ICMJE authorship criteria (see www.icmje.org) all individuals meeting the ICMJE criteria have been included as authors Note: Requests to add or remove an author after first submission will be handled according to the COPE (Committee on Publication Ethics) guidelines, set out in the COPE flowcharts (see http://publicationethics.org/files/u7140/Authorship%20A_0.pdf and http://publicationethics.org/files/u7140/Authorship%20B.pdf) COVER LETTER The cover letter addresses the following points: 1) All authors have read the submitted version of the manuscript and agreed to submit the work to JBB; 2) The work has not been published before and is not being considered by another journal; 3) The novelty and significance of the submitted work are briefly described; 4) The copyright of the manuscript will be transferred to the Society for Biotechnology, Japan (SBJ) upon acceptance for publication. LANGUAGE CORRECTION The manuscript has been checked by a professional language editing service or someone proficient in scientific English. Yes (Company or language editor’s name: please print) Author used no professional language editing service. TITLE PAGE The corresponding author is designated by an asterisk. The corresponding author's email, phone and fax numbers (including the country code) are given in a footnote. The full given name and family name (surname) of all authors are stated (middle name may be abbreviated). The complete address (city, postal code and country) for all affiliations is given. Affiliations are indicated by superscript numbers after each author name. For Review or Regular papers a running title of up to 54 letters (including spaces) has been provided. (The running title is a short title which appears at the upper corner of published article pages). STYLE The manuscript file (text body) is a Microsoft Word file. Manuscript pages have line and page numbers. 1 The manuscript conforms to the size limits for Regular papers: no more than 25 double-spaced pages of text (ca. 25 lines per page, including references). Figures and tables are restricted to essential data. For Regular papers, preferably no more than 6 figures or tables (combined). An abstract is included no more than 250 words, written in the past tense as a single paragraph Guidance on style PDF is not acceptable for typesetting. Avoid using third-level headings and section numbers. Conclusion should be included in Results and Discussion (for Regular papers). REFERENCES References are arranged in citation order (not in alphabetical order). References are indicated by numbers in parentheses in the text. SUPPLEMENTARY MATERIALS Supplementary materials are limited to illustrations, pictures, tables, audio, video, and other multimedia. All methods are included in the manuscript. Supplementary figures and tables are labeled as Fig. S1/Table S1/Movie S1. Note: Supplementary materials will not be edited by journal staff. I have read the instructions to authors and this checklist, and ensured that the manuscript meets the journal’s requirements Name: please print Date: please print 2