Peroxidase Lab
Objective: 1) Identify clues that indicate a chemical reaction is taking place. 2) Identify the
products of a reaction. 3) Classify the reaction in terms of the reaction’s energy.
Question: Will yeast react with hydrogen peroxide and if so what products are formed?
Hypothesis: If I ____(what will you do?)__________, then ____(what will happen?)_______.
Materials:
Yeast
Hydrogen Peroxide (H2O2)
2 250 mL Erlenmeyer flask
Cork Stopper
Skewer
Lighter
Procedure:
1. Add 50 mL hydrogen peroxide (H2O2) to the Erlenmeyer flask
2. Stopper the flask
3. Record Observations
4. Remove stopper from flask and insert Skewer into neck of flask (DO NOT DROP IT!!)
5. Record Observations
6. Stopper flask
7. Use the lighter to ignite the skewer, blow out the flame on the skewer
8. Remove stopper from flask and insert skewer into neck of flask (DO NOT DROP IT!!)
9. Record Observations
10. Add a small amount of yeast to the flask
11. Stopper the flask
12. Record Observations
13. After about 1 ½ to 2 minutes, carefully feel the bottom of the flask
14. Record Observations
15. Ignite the skewer and blow out the flame on the skewer
16. Remove stopper from flask and insert skewer into neck of the flask (DO NOT DROP IT!!)
17. Record Observations
18. Stopper the flask and repeat steps 15-18 three more times
Peroxidase Lab
Objective: 1) Identify ________ that indicate a chemical reaction is taking place. 2) Identify
the __________ of a reaction. 3) Classify the reaction in terms of the reaction’s _________.
Question: Will yeast __________ with hydrogen peroxide and if so what
______________ are formed?
Information:
Peroxidases are ___________ all organisms have that ___________________________
____________________________________________________________________.
Hydrogen peroxidechemical formula: _____________
physical properties: ___________________________________________________
chemical properties: __________________________________________________
Hypothesis: If I ________________________, then _____________________________.
Procedure:
1. Add 50 mL hydrogen peroxide (H2O2) to the flask
2. Stopper the flask
3. Record Observations
4. Remove stopper from flask and insert skewer into neck
of flask (DO NOT DROP IT!!)
5. Record Observations
6. Stopper flask
7. Use the lighter to ignite the skewer and then blow out
the flame on the skewer
8. Remove stopper from flask and insert skewer into neck
of flask (DO NOT DROP IT!!)
9. Record Observations
10. Add a small amount of yeast to the flask
11. Stopper the flask
12. Record Observations
13. After about 1 ½ to 2 minutes, carefully feel the bottom
of the flask
14. Record Observations
15. Ignite the skewer and blow out the flame on the
Skewer
16. Remove stopper from the flask and insert skewer into
the neck of the flask (DO NOT DROP IT!!)
17. Record Observations
18. Stopper the flask & repeat steps 15-18 three more times
Peroxidase Lab
Analysis:
Observations:
1. Why did you make observations after each step in the procedure?
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
2. What observations did you make that indicate a chemical change has taken place?
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
3. Classify the reaction as endothermic or exothermic using evidence to support your answer.
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
4. What products were formed in this reaction? Provide evidence to support your answer.
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
5. Why is it important for the stopper to be replaced quickly when it has been removed?
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
6. Complete and balance the following chemical equation based on the results from this lab.
2H2O2
Peroxidase
_______ + _______ + _______
Conclusion:
Based on the results you can see that the yeast __________ react with the hydrogen
peroxide, therefore my hypothesis was _____________.
Peroxidase Lab
Objective: 1) Identify ________ that indicate a chemical reaction is taking place. 2) Identify
the __________ of a reaction. 3) Classify the reaction in terms of the reaction’s _________.
Question: Will yeast __________ with hydrogen peroxide and if so what
______________ are formed?
Information:
Peroxidases are ___________ all organisms have that ___________________________
____________________________________________________________________.
Hydrogen peroxidechemical formula: _____________
physical properties: ___________________________________________________
chemical properties: __________________________________________________
Hypothesis: If I ________________________, then _____________________________.
Procedure & Observations
Analysis:
1. Why did you make observations after each step in the procedure?
_______________________________________________________________________
_______________________________________________________________________
2. What observations did you make that indicate a chemical change has taken place?
_______________________________________________________________________
_______________________________________________________________________
3. Classify the reaction as endothermic or exothermic using evidence to support your answer.
_______________________________________________________________________
_______________________________________________________________________
4. What products were formed in this reaction? Provide evidence to support your answer.
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
5. Why is it important for the stopper to be replaced quickly when it has been removed?
_______________________________________________________________________
_______________________________________________________________________
6. Complete and balance the following chemical equation based on the results from this lab.
2H2O2
Peroxidase
_______ + _______ + _______
Peroxidase Lab
Observations:
Procedure:
1. Add 50 mL hydrogen peroxide (H2O2)
to the flask
2. Stopper the flask
3. Record Observations
4. Remove stopper from flask and insert
toothpick into neck of flask
(DO NOT DROP IT!!)
5. Record Observations
6. Stopper flask
7. Use the candle to ignite the toothpick
and then blow out the flame on the
toothpick
8. Remove stopper from flask and insert
toothpick into neck of flask
(DO NOT DROP IT!!)
9. Record Observations
10. Add a small amount of chicken liver
to the flask
11. Stopper the flask
12. Record Observations
13. After about 1 ½ to 2 minutes,
carefully feel the bottom of
the flask
14. Record Observations
15. Ignite the toothpick and blow out the
flame on the toothpick
16. Remove stopper from flask and insert
toothpick into neck of the flask
(DO NOT DROP IT!!)
17. Record Observations
18. Stopper the flask & repeat steps
15-16 three more times
Conclusion:
Based on the results you can see that the chicken liver __________ react with the
hydrogen peroxide, therefore my hypothesis was _____________.