Supplemental Files for Herbstman et al.
Cord and Three-year Global DNA Methylation
Table S1. Comparison of those with and without cord DNA methylation and with and without three year DNA
methylation
Without Cord DNA
Methylation
N
%
With Cord DNA
Methylation
N
%
Without Three-year
Methylation
N
%
With Three-year DNA
Methylation
N
%
Maternal Education
<HS
HS
>HS
228
201
137
40.28
35.51
24.2
100
96
82
35.97
34.53
29.5
45
29
39
39.82
25.66
34.51
55
67
43
33.33
40.61
26.06
412
149
73.44
26.56
206
68
75.18
24.82
87
119
78.38
73.01
24
44
24.62
26.99
266
300
47.00
53.00
120
159
43.01
56.99
48
66
42.11
57.89
72
93
43.64
56.36
507
56
90.05
9.95
255
23
91.73
8.27
100
13
88.5
11.5
155
10
93.94
6.06
355
210
62.83
37.17
193
86
69.18
30.82
82
32
71.93
28.07
111
54
67.27
32.73
35
268
115
148
6.18
47.35
20.32
26.15
14
125
62
78
5.02
44.8
22.22
27.96
5
51
22
36
4.39
44.74
19.3
31.58
9
74
40
42
5.45
44.85
24.24
25.45
189
369
33.87
66.13
93
181
33.94
66.06
35
77
31.25
68.75
58
104
35.8
64.2
117
394
22.9
77.1
47
212
18.15
81.85
21
86
19.63
80.37
26
126
17.11
82.89
222
228
N
557
557
406
521
434
49.33
50.67
mean (SD)
63.0 (4.0)
146.0 (37.3)
36.8 (71.0)
25.2 (4.9)
2.34 (2.03)
130
149
N
277
270
252
279
268
46.59
53.41
mean (SD)
63.7 (3.5)
151.1 (38.1)
34.3 (16.1)
25.1 (4.8)
2.44 (2.29)
52
62
N
112
111
100
100
114
45.61
54.39
mean (SD)
63.0 (3.8)
148.8 (36.3)
30.9 (15.8)
2.3 (2.5)
25.0 (4.7)
78
87
N
165
159
152
159
165
47.27
52.73
mean (SD)
64.1 (3.2)
152.7 (39.3)
36.5 (16.0)
2.5 (2.1)
25.1 (4.9)
Marital Status
Married/Cohab.
Unmarried
Material Hardship
Yes
No
Public Assistance
Yes
No
Ethnicity
Dominican
African American
BMI
Underweight
Normal weight
Overweight
Obese
ETS
Yes
No
Former smoker
Yes
No
Sex
Male
Female
Maternal Height (in)
Pre-pregnancy weight
Weight gain
Maternal Age
Total PAH*
Supplemental Files for Herbstman et al.
Cord and Three-year Global DNA Methylation
Table S2. Factors predicting absolute value of the change in DNA methylation over time (change = |log-three year meth
– log-cord meth|)
Beta
Maternal Education
HS (vs. no HA)
>HS (vs. no HA)
Married/Cohab. (vs. unmarried)
Material Hardship
African American (vs. Dominican)
Prenatal ETS
Former smoker
Male (vs. female)
Multiparous (vs. nulliparous)
Pre-pregnancy BMI
Weight Gain
Maternal Age
Total PAH (ln-adjusted)
Breast Fed
ETS at 3 yrs
Cord methylation
Birthweight
Gestational age
R2
Adjusted R2
-0.213
-0.660
-0.005
0.056
0.285
0.097
-0.588
0.005
0.066
-0.056
-0.008
-0.022
0.142
0.029
-0.185
-0.011
0.000
-0.091
0.140
0.000
Lower
95% CI
-0.921
-1.465
-0.751
-0.546
-0.449
-0.638
-1.323
-0.579
-0.621
-0.108
-0.029
-0.099
-0.269
0.000
-0.978
-0.239
-0.001
-0.333
Upper
95% CI
0.496
0.145
0.741
0.659
1.020
0.832
0.146
0.588
0.753
-0.004
0.012
0.055
0.553
0.057
0.607
0.218
0.001
0.151
Supplemental Files for Herbstman et al.
Cord and Three-year Global DNA Methylation
Figure S1. Partial regression plots from multiple regression analysis that test the
association between DNA methylation (cord or three year, as indicated) and pre-pregnancy maternal body mass index
(BMI).
A. All cord DNA methylation
B. Cord DNA methylation (subset with measured three year DNA methylation)
Supplemental Files for Herbstman et al.
Cord and Three-year Global DNA Methylation
C. Three year DNA methylation
D. Three year DNA methylation, adjusting for cord DNA methylation