IMPAQ Work Package 1
WP nr.
WP responsible
Objectives:
(could be divided
into tasks- if so use
one form per task)
WP1 – 25-03-2011
Josianne Støttrup
Technical improvement of copepod production system (indoor and outdoor)
Task 1.1. Indoor culture. Engineers develop system based on known technology
(AKVAgroup, RUC, ISPRA).
Task 1.2. Outdoor culture. Engineer and biologists (Maximus, Fish-Lab, NERI, RUC,
DTU Aqua).
Milestones (list
these for the WP
or WT with months
for deliverables)
Activities planned
project
Year 1
1.1. Establish intensive system + manuals
1.2. First year experiments + manual
1.1. Establish system. Delays due to problems with tender, but system expected
established end of summer. Experiments with A. tonsa to establish tolerance
levels for ammonia.
1.2. First year experiments:
Suggestion: Aim to establish seasonal dynamics of phyto- and zooplankton
development in extensive systems. Compare spring and summer situation(s).
Four tanks to be set up in each campaign. Start up should take place at the same
time, if possible with the same batch of larvae to reduce one of the variables.
Suggest to try this out in Maximus in the large tanks, and plan during 2011 the
experiments on Venøsund and Maximus for 2012.
Monitor
A. Abiotic parameters such as temp. Sal, Oxygen, pH, light
B. Phytoplankton qualitative and quantitative
C. Zooplankton qualitative and quantitative
D. Nr. Fish larvae delivered to system and fry output
E. Fish larvae physiology
Participation and resources allocated for 2011.
Budgeted man months for the first year – WP 1.
P.Nr Inst.
PhD
Post doc
Researcher
0
RUC
8
1
1
DTU Aqua
1
2
DVDB KU Life
3
ISPRA
4
NTOU
5
ULST
6
FishLab
1
7
Venø
8
AKVA group
9
Maximus
10
IFRE (KU)
11
NERI (AU)
1
Please refer to detailed plan for outdoor campaigns.
TAP
Input to this WP or WT.
Indoor + Outdoor system
WP lead mostly
Outdoor system
2
Technical Assistance
Outdoor system
Experiment for optimizing of outdoor copepod system
Year 1: Comparative study of the seasonal differences fish larvae (Turbot) fitness related to composition of
phytoplankton and zooplankton communities. Emphasizing the spring and summer blooms.
Fish stocking: mid May, late June, August, September.
Description: The experiment will be performed in 4 x 200m3 ø12 x 2,5m outdoor concrete ponds with
pretreated water. The intention is to monitor the growth and survival of the turbot larvae compared to the
quantity and quality of phytoplankton and zooplankton.
For now the tanks are filled with prefiltered seawater and left for bloom 1 week before stocking of fish
larvae, around 10 May. They are monitored daily for oxygen and secchi-depth. When quantity of the
phytoplankton and temperature are evaluated to be sufficient by measures of the secchi-disk and
thermometer the tanks are stocked with turbot larvae. Each tank is stocked with around 30.000-40.000
turbot larvae. The fish larvae are grown in 5 weeks before they are transferred to the indoor systems, for
on-growing.
Researcher allocation:

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Maximus are responsible for Abiotic factors supported by Per (CTD/chlorophyll/HOBO) (70
samples).
Fish-Lab is responsible for phytoplankton analysis (20 samples).
RUC is responsible for zooplankton samples and fatty acid (2 x 80 samples).
DTU-Aqua is responsible for Fish larvae sampling supported by Per (40 samples + initial and final)
Environmental factors:
Abiotic factors – pH (RUC), Light – HOBO-loggers in tank and in air (RUC), Oxygen/Temperature (Maximus),
Secchi-disk (Maximus), (Sampling every second day).
CTD + insitu fluorometer to analyze plant pigment, salinity and temperature (sampling every second day)
Phytoplankton:
Sampling by 3 L heart valve water sampler (Once a week). Size fractionated chlorophyll (<20, 20-50, >50um,
Total) (RUC). Algae identification and quantification by inverted microscopy (Fish-Lab).
Zooplankton:
Sampling twice a week by zooplankton pump, 120L (Maximus?), alternatively by 50um mesh size plankton
net hauls (sampling on-site conditions?). Every sample is spitted by Folsom plankton splitter into 2 equal
subsamples (RUC).
Subsample 1 – Zooplankton identification:
Divided into two different size fractions >200um and <200um. Each fraction is counted and species and
development stage determined (RUC).


Copepods,( <200um) nauplii Stage 1-3 and 4-6,( >200um) copodites and adults (RUC/Fish-Lab).
Rotifers ( <200um) (RUC/Fish-Lab)
Subsample 2 – Zooplankton fatty acid analyzes:
Divided into two different size fractions >200um and <200um. Each fraction is processed for GCMS (RUC).
Fish larvae:
Initial stocking & end of experiment (number, weight, length) (DTU-Aqua/Maximus/RUC).
Sampling of fish larvae twice a week (Is it realistic to obtain a quantitative measurement?)(Net hauls,
pumps, size fraction sorting?)

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Counted, initial and terminal (population survival)
Individual length measure (notochord) and individual weight measure
Fatty acid analysis
Metamorphosis success
Pigmentation
The experiment are indented to be replicated at least ones more. Maximus intent to stock 4 batches in
2011 (10 May, 20 June, 1 August and 1 September). Dependent on weather and batch survival.