OWL PELLET AND ENERGY PYRAMID LAB Online You will need a camera for this lab. Main heading for your post: Owl Pellet and Energy Pyramid Lab Subheadings: Part 1 Owl Pellet Dissection, Part 2 Ecological Pyramids See what to submit under each part. You will only work with one pyramid in Part 2. Objectives 1. 2. 3. 4. 5. 6. 7. 8. Demonstrate an understanding of the nature of science and the scientific process. Classify animals according to type of skeletal structure. Identify bones that compose the skeletal system. Trace the flow of energy through the trophic levels and energy pyramids. Explain how organisms interact within an ecosystem and how owls affect an ecological community. Demonstrate analysis of data to produce visual results and written explanations. Perform calculations to determine patterns. Demonstrate collaborative skills. Introduction Owl pellets are the undigested remains of prey ingested by an owl. The owl swallows its prey whole, and during the process of digestion, the soft parts of the prey are dissolved and passed on to the intestine for absorption. The hard, non-digestible parts-bones, teeth, fur, feathers, and chitonous remains of insects are compressed in the gizzard and passed on to the proventriculus, the first chamber of the owl’s stomach, where the pellet remains until it is expelled. These pellets are not eliminated as feces, but are regurgitated through the mouth. The Common Barn Owl feeds in early morning and early evening and will usually produce one to two pellets per day. Glossy black when fresh, the pellet remains smooth and dark in color when dry. Your pellet has been fumigated to eliminate the presence of any insects and then individually wrapped for preservation. Unless otherwise stated, the pellets in this lab are from the family Tytonidae and more specifically, the Common Barn Owl, Tyto alba. View this video to learn more about owls and pellets, http://youtu.be/JdrbSzC6Jcg. View these slideshows to learn more, http://www.barnowltrust.org.uk/infopage.html?Id=193. Safety 1. Be careful with sharp tools and bones. 2. Wash hands after activity. Materials Owl pellet Camera Dissecting surface Scale Bone sorting charts Other small tools, as supplied Glue Sheet of plain paper or cardboard for mounting bones Part 1 Owl Pellet Dissection What to submit for this part: o Table 1 (1 points) o Photo of chart with sorted bones (2 points) o Photo of reconstructed skeleton (2 points) o Table 2 (1 points) o Graph of species numbers (2 points) o Comments about other species found Procedure 1. Remove the pellet from the aluminum foil. 2. Measure the length and width of the pellet and record these measurements in Table 1. Table 1. Owl Pellet Measurements Length Width 3. Place the pellet on the dissecting surface. Carefully separate the bones from fur or feathers onto another part of your surface using your fingers and available tools. 4. Carefully clean the bones by picking or scraping off debris and sort them according to type—skulls, jaws, vertebrae, etc. 5. When you are sure that all bones have been separated, dispose of the other materials in the trash. 6. Identify the animals found in your pellet by matching the bones in your pellet with those in the Bone Sorting Chart. 7. Place each bone beside the matching bone on the Bone Sorting Chart.. You may have many of each type of bone sorted and glued or just a few. 8. Take a photo of your chart with the sorted bones. 9. Reconstruct a partly complete skeleton of one prey animal. Glue the parts on a sheet of paper and take a photo. 10. Record the types and numbers of animals in Table 2. Table 2. Kinds and Number of Animals Found in Owl Pellets No. Voles No. Shrews No. Birds Your Data 11. Make note of any other species you can identify. 2 No. Moles No. Rats 12. Construct a bar graph of the data in Table 2, using the grid below or another source. Part 2 Ecological Pyramids The amount of energy or matter in an ecosystem can be illustrated by a drawing called an ecological pyramid. In this activity you will construct three types of ecological pyramids, a number pyramid, a biomass pyramid, and an energy pyramid. Each pyramid you construct will consist of four trophic levels. The top of each will relate to your owl, the only tertiary consumer (3o). The next level down will relate to the owl’s prey, the secondary consumers (2o). For the purposes of this activity, assume the prey found in the owl pellets are secondary consumers. The next trophic level down will relate to the organisms eaten by the owl’s prey, the primary consumers, (1o). The base of each pyramid will relate to the organisms eaten by the primary consumers, the producers. Use the information obtained from your owl pellet dissection to construct the ecological pyramids below. A. Number Pyramid for a 24-hour Period Only students with last names starting with A through H perform this part. What to submit for this part: o A drawing or depiction of the completed number pyramid. You may not copy and paste someone else’s pyramid. (5 points) o Calculations for number 3, 4, and 6 (3 points) o Answers to questions 1-4. (4 points) A number pyramid is an ecological pyramid that indicates the total number of organisms in each trophic level. 1. Draw your pyramid on a separate piece of paper, labeling the number of 3o, 2o and 1o consumers. Reminder, there is only 1 owl, which is the 3o consumer. 2. From the number of skulls in your pellet (same type or different types) determine the number of prey consumed. One skull = one prey animal. 3. Determine the ratio, R, of the number of 2o consumers to the number of 3o consumers. R = # 2˚ Consumers 3 # 3˚ Consumers 4. Use R to calculate the number of 1o consumers by multiplying the number of 2o consumers by R. # 1o Consumers = R (# 2˚ consumers) 5. Label your pyramid with the number of 1o consumers. 6. Calculate the number producers by multiplying the number of 1o consumers by R. # Producers = R (# 1˚ consumers) 7. Finish your pyramid by labeling it with the number of producers. 1. What are owl pellets? 2. During the nesting season the young need an enormous amount of food for growth. If the nest contains three young and each of the young eat five mice per night for a month and the two adults eat four per night, how many mice would the parents have to capture in 30 days? 3. The pellets are from what owl family? 4. Environmentalists are concerned about the accumulation of certain poisons in predators. Why do you suppose owls, eagles, swordfish, humans and lions are often the most threatened organisms of a community that are exposed to DDT or mercury? B. Biomass Pyramid for a 2.5-year Lifespan. Only students with last names starting with I through P perform this part. What to submit for this part: o Completed Table 3 (3 points) o Drawing or depiction of your biomass pyramid. You may not copy and paste someone else’s pyramid. (2 points) o o Calculations for number 3, 5, and 7 (3 points) Answers to questions 1-4 (4 points) A biomass pyramid is an ecological pyramid that indicates the total mass of organisms in each trophic level. 1. Complete Table 3. Table 3 A B C Prey types Biomass No. found in pellet House Mouse 20 g Vole 40 g D= C x 365 No. eaten per year (1 pellet per day for 365 days) 4 E= BxC F= E x 365 Biomass eaten per 24 hours (g) Biomass eaten per year (365 days) (g) G= F x 2.5 Biomass eaten over a 2.5 year lifespan (g) Shrew 5g Brown Rat 240 g Bird 80 g Other Total 2. Draw your biomass pyramid on a separate piece of paper. Label the total biomass of the 3o consumer. Assume the owl’s biomass is 500 g. 3. Determine the new ratio, R, of the total biomass of the 2˚ consumers to the total biomass of the 3˚ consumers. R = Biomass of 2˚ consumers Biomass of 3˚ consumers 4. Add the biomass of the 2o consumers to your pyramid. 5. Use your ratio, R, to calculate the total biomass of the 1˚ consumers by multiplying the total biomass of the 2˚ consumers by R. Biomass of 1˚ consumers = R (biomass of 2˚ consumers) 6. Add the total biomass of the 1˚ consumers to your pyramid. 7. Calculate the total biomass of the producers by multiplying the total biomass of the 1˚ consumers by R. 8. Finish your pyramid by labeling it with total biomass of the producers. Answer these questions. 1. What happens to the soft parts of the prey? 2. These pellets are not eliminated as _________________, but are regurgitated through the ___________________. 3. In terms of energy flow in an ecosystem, why is the amount of energy passed from one trophic level to the next so small? Since we know energy cannot be destroyed, account for the loss of energy that occurs between the prey and the owl. 4. Describe any pattern you see. C. Energy Pyramid for a Trophic Level. Only students with last names starting with Q through Z perform this part. What to submit for this part: o Drawing or depiction of your energy pyramid. You may not copy and paste someone else’s pyramid. (5 points) 5 o o Calculations for number 2 and 3. (2 points) Answer questions 1-5 (5 points) An energy pyramid is an ecological pyramid that indicates the amount of energy in each feeding level. According to the terms of thermodynamics, only about 10% of the energy available within one trophic level is transferred to organisms at the next trophic level. 1. Assume that the producers in this pyramid (plants) contain a total of 500,000 Calories of food energy. Draw an energy pyramid and label the base with this value. 2. Calculate the amount of energy for the 1˚ consumers by multiplying the food energy of the producers by 0.10. Write this value in your pyramid. 3. Complete your energy pyramid by calculating and labeling the energies for the top two trophic levels. Assuming that the barn owl regurgitates one pellet per day, how many prey items would the owl that produced your pellet consume per year? Answer these questions. 1. Where does the pellet remain until it is expelled? 2. List 6 other birds that are known to regurgitate pellets. 3. Placing the owl at the highest trophic level, create a food web using the following items: vole, deer mouse, mole, house mouse, weasel, shrew, snake, starling, frog, salamander, spider, grubs, earthworms, centipedes, crane fly, seeds, plants, roots. 4. Why does the number of organisms decrease as you move up the pyramid level? 5. We know that about 50% of the energy in an organism is not digested by the consumer and is simply passed through as waste or feces. Is the energy stored in the feces lost to the ecosystem? Why or why not? 6 How to measure the jaw Tooth types Jaw Length Lobed Angled Pointed Rat Vole Mouse Shrew Bird Jaw Length (mm) 17-30 15-20 10-15 7-14 15-40 Tooth Type Lobed Angled Lobed Pointed None Skull and Jaw Shoulder Blade Hip Upper Leg Lower Leg Rib Vertebra Foot 2