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SUPPLEMENTAL FIGURE LEGENDS
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Figure S1. Bc infection in plants grown at high density. A) Lesion diameters scored 3dpi on leaves
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inoculated with a 5µl droplet of 5x105 Bc spore solution. I: lesion diameter < 1.5 mm, II lesion diameter ≥
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1.5 mm and ≤ 3 mm, III: lesion diameter > 3 mm (n > 20 leaves). B) Representative photographs of
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inoculated leaves from plants grown individually (single) or at high-density canopies.
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Figure S2. Leaf response to Bc-inoculation. Relative expression of PDF1.2 in Bc-inoculated petioles and
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leaf laminas 2dpi (n = 3). Data represent means ± SE. Asterisk indicates significant difference (Student’s t-
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test, p < 0.05).
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Figure S3. Disease incidence of the constitutive defence overexpressors cpr1 and cev1 in control light
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and low R:FR. A) Bacterial growth quantification 3dpi of Pst-inoculated leaves. Cfu = colony forming
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units. B) Lesion diameters scored 3dpi on leaves inoculated with a 5µl droplet of Bc spore solution. As
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average lesions size was much smaller in cev1 mutants (inset), different classes were used for Col-5 and
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cev1 to analyse low R:FR effect. For Col-5 I: lesion diameter < 4 mm, II: lesion diameter ≥ 4 mm and ≤ 8
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mm, III: lesion diameter > 8 mm. For cev1 I: lesion diameter < 1.5 mm, II: lesion diameter ≥ 1.5 mm and ≤
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3 mm, III: lesion diameter > 3 mm (n > 20 leaves).
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Figure S4. Overlap in differentially expressed genes induced by 2h combined treatment of SA and low
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R:FR and combined treatment of MeJA and low R:FR. The one overlapping gene is PHYTOCHROME
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RAPIDLY REGULATED1 (PAR1), which just falls short on the statistical cut off used for this dataset (low
1
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R:FR-induced adj.p = 0.005) and is a well-known low R:FR-induced gene (Roig-Villanova et al., 2007;
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Sessa et al., 2005).
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Figure S5. Heatmap of log2 Fold Change expression values of JAZ genes in MeJA treatment and MeJA
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combined with low R:FR. All presented JAZ genes were significantly differentially expressed in both
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treatments as compared to control except for JAZ1, which was not differentially induced by MeJA alone.
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Figure S6. Volcano plots of JA and SA microarrays, representing the significance (log odds; the probability
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that a gene is differentially expressed) and fold change for all genes.
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SUPPLEMENTAL TABLE LEGENDS
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Table S1. GO analysis of differentially expressed genes from SA- and MeJA microarrays. Adjusted p-
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values for enriched GO categories are depicted for separately analysed subgroups from the venn
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diagrams in Fig. 4).
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Table S2. Gene IDs corresponding to Fig. 5C and D.
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Table S3. SA-induced kinases repressed by low R:FR.
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Table S4: Primer sequences (5’→3’) used for Real-Time RT-PCR. F: forward, R: reverse.
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Table S5: Primer sequences (5’→3’) used for semi-quantitative RT-PCR. F: forward, R: reverse.
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2
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3