Supplementary Figure 1. Technical validation of NanoString gene expression data by
quantitative real time PCR (qRT-PCR).
Technical validation of 8 genes from the 11-gene signature was performed using qRT-PCR.
Expression of the 8 genes was normalized to reference genes. Relative expression levels of the 8
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differentially expressed genes are shown in the resistant compared to the sensitive cohort. Data
analysis was performed using relative quantification by the ΔΔCt method.
Supplementary Figure 2. Western blot analysis showing STAT1 antibody specificity.
Total protein extracts from four different ovarian cancer cell lines, 3133, 3392, 2223, 3331 and
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OVCAR3 were loaded on 10% SDS-PAGE gel followed by hybridization using polyclonal
rabbit anti-human STAT1 antibody.
Supplementary Figure 3. Receiver operative characteristic curves
a) ROC curve for STAT1 for the progression of ovarian cancer. The area under the curve
(AUC) is 0.636 with p-value=0.006. b) ROC curve for STAT1 for the overall survival from
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ovarian cancer. The area under the curve (AUC) is 0.555 with p-value=0.200. The statistics
were done using SPSS assuming the smaller test result indicate a more positive test. The
diagonal line corresponds to random chance.
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