Digital Unit Plan Template Unit Title: Protein Power Content Area: Biology (Cell and Molecular Biology) Name: Jessica Malinchak th th Grade Level: 9 -12 grade CA Content Standard(s)/Common Core Standard(s): HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells. HS-LS1-2. Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms. HS-LS1-3. Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis. Big Ideas: Essential Question: What is the power of protein? What is protein and why is it important? Unit Question: How is protein structure related to its function? Content Question: How is protein synthesized? Unit Goals and Objectives: 1. Students will be able to list and summarize the two steps (transcription and translation) of the central dogma in writing. 2. Students will be able to compare the steps and molecular components of transcription and translation using a graphic organizer. 3. Students will be able to categorize the structures (primary, secondary, tertiary, and quaternary) and functions (enzyme, antibody, DNA-associated, contractile, structural, hormone, or transport) of given proteins when given a worksheet. 4. Students will be able to predict the function of a protein when given background information and the structure of the protein. 5. Students will be able to practice protein lab techniques in class and during lab homework assignments. 6. Students will be able to explain the importance of protein by creatively designing a group research project to investigate and share the importance of a specific protein. Unit Summary: In this unit, students will engage in class activities that will help them understand the power of protein! Together, we will identify common misconceptions about protein and develop a proper understanding of the importance, make-up, structure, function, and synthesis of protein. The first lesson will begin with a class discussion of protein within our everyday life and a detailed overview of the central dogma steps that allow for the synthesis of protein. The second lesson allows students to utilize 21st century technology skills by investigating information on reliable science websites about protein structure and function. The third lesson helps students organize important protein lab procedures and results into graphic organizers. At the end of the unit plan, students will be able to work collaboratively in pairs and groups to hypothesize the function of a protein and research the importance of a protein. Assessment Plan: Entry-Level: 1. Communication Strategy (Criss Cross Puzzle): Each student will be given a criss cross puzzle or can download the file from the unit plan site. At the beginning of the unit plan before any lessons, students will be asked to review the important parts of an eukaryotic cell using the criss cross puzzle. This activity can be completed individually or with a partner. This activity will help students to tap into prior knowledge about the parts of the cell. Formative: 1. Organization Strategy (Guided Notes): During the lecture 1 presentation, students will have guided notes which will be completed as the lecture progresses. The guided notes consist of a chart with pictures, questions, and space for notes. As the teacher asks students to find these features during the presentation, students will write important pieces of information in the space provided. During the lesson, the teacher will check to see if the students have identified the correct parts of protein Summative: 1. Collaboration Strategy (Research Project): After lesson 3, students will be asked to work in groups of 4 or 5 to research and present on a specific protein that can cause a disease when mutated or missing. Students 2. Interaction Strategy (Prediction Circles): Each student will have a picture of an eukaryotic cell with labeled cellular components on the guided notes. At the beginning of lesson 1, students will be asked to predict which parts of the cell are involved in making protein by circling and briefly explaining with one sentence. This activity can be completed individually or with a partner. This activity will help students to tap into prior knowledge about the parts of the cell to be able to visualize protein synthesis within the cell. 3. Communication Strategy (Class Discussion): During the beginning of lesson 1, students will be asked to participate in a class discussion that involves defining "protein" and sharing ideas as to why protein is important within biology and real world experiences. This activity will help students to tap into prior knowledge about protein so synthesis by walking around the room and checking their guided notes throughout the presentation. 2. Presentation Strategy (Digital Poster): Protein synthesis terms will be defined during lesson 1 and students will be able to write about the terms using the guided notes. For extra practice with the new terms, students will be directed to complete a group digital poster on the Padlet Site towards the end of the lesson. Students will be separated into groups of 3 and given 10 minutes to create a digital poster for one given term using class computers. Students will need to use visuals and/or videos with text to define the specific term. This activity will help students to practice vocabulary, strengthen technology skills, and create multimedia examples of vocabulary words for the class to use as extra review for the short essay assessment at the end of the unit plan. Click the green Padlet Site word above to go to the proper site. 3. Communication Strategy will be given: 1. a set of guidelines for the research and presentation and 2. a rubric that shows students how the presentation (content and skills) will be evaluated. Student s will use Google docs to organize and present research. 2. Interaction Strategy (Short Essay): After the research project, a final short essay assessment will be given to students to assess the acquisition of the big ideas of the unit plan. Students will be given a prompt that they will be able to understand the relevance of protein within real world experiences. 4. Collaboration Strategy (Class Discussion): During the beginning of lesson 3, students will be asked to participate in a class discussion that involves identifying correct and incorrect lab safety techniques. Pictures of Walt from Breaking Bad and George W. Bush within the lab will be displayed within the short teacher lecture. Students will be given time to analyze the pictures and identify good and bad lab technique. This activity will help students to tap into prior knowledge about lab safety so they will be able to understand the importance of proper lab technique. (Poem/Rap): After lesson 1, students will be told to listen to the protein synthesis rap on the last slide of the lecture and to read the lyrics on the guided notes. This rap is used to show students an example of a creative summary and to inspire them to write their own rap/poem that summarizes and compares transcription and translation as a homework assignment. The creative summary needs to be at least five full sentences. It doesn’t have to rhyme but extra credit points will be given to fun and creative work! This assignment will help students practice summary writing. 4. Collection Strategy (Webercise): During lesson 2, the webercise will be completed. This activity will require students to access given internet sites and answer questions about the information provided on the sites. The questions in the webercise are separated by topic and site. The webercise will be completed outside and inside of class time. describes a specific protein's structure, gives clues to the protein's function, and explains the harmful effects when the protein is mutated. Students have to predict the function of the protein and describe how the protein obtained mutations by outlining and explaining the steps of protein synthesis. Key vocabulary terms must be used properly for students to receive full credit. 5. Communication Strategy (Flash Cards): After lesson 2, students will be asked to take a protein vocabulary flash card quiz on the quizlet site. This quiz is used to check if students are forming the targeted protein concepts as intended. Click the green quizlet site word above to go to the flash card quiz. 6. Organization Strategy (Lab Graphic Organizers): During the lesson 3 lab activity, students will have a lab report worksheet to complete. The lab report involves organizing lab procedures and results by using a graphic organizer. Students can work together to complete it but they need to fill it out and turn it in individually. The worksheet includes assignment directions, timeline for completing the assignment, how the assignment helps students achieve content standards, and how the assignment fits into the digital unit assessment plan. A sample student graphic organizer is given to reflect what is expected of a highly scored assignment. The scoring rubric is also given to show students how the content and format of the graphic organizers will be graded. Lesson 1 Student Learning Objective: 1. Students will be able to understand the importance of protein by explaining at least one protein function (enzyme, antibody, DNAassociated, contractile, structural, hormone, or transport). Acceptable Evidence: This lesson will require students to communicate, interact with, present, and organize information provided within the lecture. Acceptable evidence of student understanding can be 2. Students will be able to collected and list and summarize the measured in two steps (transcription students’ completion and translation) of the of the guided notes. central dogma in writing. 3. Students will be able to define the steps and molecular components of transcription and translation. Instructional Strategies: ☐ Communication ☐ Collection ☐ Collaboration ☐ Presentation ☐ Organization ☐ Interaction Lesson Activities: Interaction Strategy (Prediction Circles): Each student will have a picture of an eukaryotic cell with labeled cellular components on the guided notes. Students will be asked to predict which parts of the cell are involved in making protein by circling and briefly explaining with one sentence. This activity can be completed individually or with a partner. This activity will help students to tap into prior knowledge about the parts of the cell to be able to visualize protein synthesis within the cell. Communication Strategy (Class Discussion): Students will be asked to participate in a class discussion that involves defining "protein" and sharing ideas as to why protein is important within biology and real world experiences. This activity will help students to tap into prior knowledge about protein so they will be able to understand the relevance of protein within real world experiences. Presentation Strategy (Digital Poster): Protein synthesis terms will be defined during the lesson and students will be able to write about the terms using the guided notes. For extra practice with the new terms, students will be directed to complete a group digital poster on the Padlet Site. Students will be separated into groups of 3 and given 10 minutes to create a digital poster for one given term using class computers. Students will need to use visuals and/or videos with text to define the specific term. This activity will help students to practice vocabulary, strengthen technology skills, and create multimedia examples of vocabulary words for the class to use as extra review. Presentation Strategy (Interactive Lecture): The teacher will show a prezi presentation about protein synthesis. The presentation will progress through a series of questions which ask students to think critically about the information provided. Students will write answers and thoughts to the questions in their guided notes. Organization Strategy (Guided Notes): During the lecture presentation, students will have guided notes which will be completed as the lecture progresses. The guided notes consist of pictures, questions, and space for notes. As the teacher asks students to find these features during the presentation, students will write important pieces of information in the space provided. Communication Strategy (Poem/Rap): Check for understanding through a creative summary assignment. Students will listen to the rap on last slide of the lecture and then write their own rap/poem that summarizes and compares transcription and translation. The creative summary needs to be at least five full sentences. It doesn’t have to rhyme but extra credit points will be given to fun and creative work! This assignment will help students practice summary writing. Lesson 2 Student Learning Objective: Acceptable Evidence: 1. Students will be able to list and summarize the two steps (transcription and translation) of the central dogma in writing. This lesson will require students to communicate, collect, collaborate, interact with, and organize Instructional Strategies: ☐ Communication ☐ Collection ☐ Collaboration ☐ Presentation ☐ Organization ☐ Interaction Lesson Activities: Communication, Collection, Collaboration, Organization, and Interaction Strategy (Webercise): The webercise will be completed outside and inside of class time. Webercise questions from the topics Importance of Protein, 2. Students will be able to compare the steps and molecular components of transcription and translation using a graphic organizer. 3. Students will be able to categorize the structures (primary, secondary, tertiary, and quaternary) and functions (enzyme, antibody, DNAassociated, contractile, structural, hormone, or transport) of given proteins when given a worksheet. information provided within the web-based learning activity (webercise). Acceptable evidence of student understanding can be collected and measured in students’ completion of the webercise. Protein Diet, Basics of a Protein, and Protein Code will be completed before class as homework. Webercise questions from the topics Protein Synthesis (Translation), Build A Protein, Build Your Protein Name Quiz, Protein Structure, Protein Functions, and Mutated Proteins will be completed during pair and/or group class work. Lastly, webercise questions from the topic Test Protein Knowldege will be completed after class as homework. This webercise will require students to access given internet sites and answer questions about the information provided on the sites. Students must interact with specific websites to collect the important information for the webercise worksheet. Once the students collect the information, students must communicate and organize the important information in their own words and within a clear easy to follow format. This activity can be completed individually or in collaboration with a partner. 4. Students will be able to predict the function of a protein when given background information and the structure of the protein. Lesson 3 Student Learning Objective: Acceptable Evidence: 1. Students will be able to practice a protein lab This lesson will require students to Instructional Strategies: ☐ Communication ☐ Collection ☐ Collaboration Lesson Activities: Collaboration Strategy (Class Discussion): Students will be asked technique in class. collaborate, present, and organize 2. Students will be able to information provided organize protein lab within the protein lab protocol and results into activity. Acceptable graphic organizers. evidence of student understanding can be 3. Students will be able collected and to predict the function of measured in a protein when given students’ completion background information of the pre-lab and the structure of the worksheet and lab protein. graphic organizers with post-lab 4.Students will be able questions worksheet. to explain the importance of protein by creatively designing a group research project to investigate and share the importance of a specific protein. ☐ Presentation ☐ Organization ☐ Interaction to participate in a class discussion that involves identifying correct and incorrect lab safety techniques. Pictures of Walt from Breaking Bad and George W. Bush within the lab will be displayed within the short teacher lecture. Students will be given time to analyze the pictures and identify good and bad lab technique. This activity will help students to tap into prior knowledge about lab safety so they will be able to understand the importance of proper lab technique. Presentation Strategy (Interactive Short Lecture): The teacher will show a short prezi presentation about the lab activity. The presentation will include safety, purpose, procedure, and results of the lab technique. The students' participation will be necessary during the short lecture to ensure successful completion of the lab activity. Organization Strategy (Pre-Lab Worksheet): During the lecture presentation, students will have a pre-lab worksheet to be completed as the short lecture progresses. The pre-lab worksheet consists of empty boxes to be filled in with lab safety and ELISA information. Students can work together to complete it but they need to fill it out individually. Collaboration Strategy (Lab Activity): Students will work in groups of 3-4 to complete the ELISA technique experiment. Students will use the pre-lab worksheet and the lab protocol to help complete the lab activity. Students will use the graphic organizer lab report to document and organize lab procedure and results. Organization Strategy (Lab Graphic Organizers): During the lab activity, students will have a lab report worksheet to complete. The lab report involves organizing lab procedures and results by using a graphic organizer. Students can work together to complete it but they need to fill it out and turn it in individually. The worksheet includes assignment directions, timeline for completing the assignment, how the assignment helps students achieve content standards, and how the assignment fits into digital unit assessment plan. A sample student graphic organizer is given to reflect what is expected of a highly scored assignment. The scoring rubric is also given to show students how the content and format of the graphic organizers will be graded. Collaboration Strategy (Post Lab Questions): Check for understanding through post lab questions. Students can work individually or in a group to answer the questions. These questions will be given as homework if the students don't have time to finish in class. Unit Resources: Books 1. Campbell, Neil A., and Jane B. Reece. "Chapter 3 and 10." Essential Biology. San Francisco: Benjamin Cummings, 2001. Print. 2. Freeman, Scott. "Chapter 13: Transcription and Translation." Biological Science / Scott Freeman. Upper Saddle River, NJ: Pearson Prentice Hall, 2005. Print. 3. Reece, Jane B., and Neil A. Campbell. "Chapter 17: From Gene to Protein." Campbell Biology / Jane B. Reece. Boston: Benjamin Cummings, 2011. Print. Websites 1. "ELISA Immuno Explorer Kit." Bio-Rad Laboratories Inc. USA, 2014. Web. Apr. 2014. 2. Manning, M.D., Michael."ELISA Tests: Read About the Blood Test and Antibodies." MedicineNet Inc. Web. Apr.2014. 3. National Science Teacher Association. "Books & Resources." NSTA: safety in the Science Classroom. Web. Apr.2014 4. "Protein Synthesis Animation Video." YouTube. YouTube, 04 Aug. 2010. Web. 5. "Protein Synthesis Rap." YouTube. YouTube, 05 May 2013. Web. 6. "Transcription." YouTube. YouTube, 13 Nov. 2006. Web. Useful Websites: Protein in Everyday Life: The Healthy Eating by Demand Media Site provides healthy eating options including the importance of high protein meals. This site helps students understand how protein is involved in our everyday life. The Protein and the Teen Athlete Site provides information on the importance of protein for a teen athlete. This site gives readers an equation to use to calculate how much protein is necessary for a certain body weight and activity level. Protein Building Blocks: The John Kyrk Site provides great visuals that allow students to compare the molecular makeup of amino acids, DNA, and RNA. This site also allows students to see which molecules are involved during the two steps of the central dogma, transcription and translation. Protein Structure: The Chem4Kids Site provides information and clear visuals of the four structures of protein (primary, secondary, tertiary, and quaternary structure). Protein Function: The Science Learning Site provides a list and explanation of protein functions within the body. Transcription: The Pearson Site provides a BioCoach activity that will help students review transcription, the synthesis of RNA from DNA. This site provides text, visuals, interactives, and quizzes. The Howard Hughes Medical Institute (HHMI) Site provides a detailed animation and explanation of transcription. The Scitable by Nature Education Site provides great reading material that explains transcription. The site gives analogies, examples, and graphic visuals of transcription. The McGraw-Hill Site provides an animation of mRNA synthesis (transcription) along with quizzes to test student understanding of transcription. Translation: The Pearson Site provides a BioCoach activity that will help students review translation the synthesis of protein from RNA. This site provides text, visuals, interactives, and quizzes. The Howard Hughes Medical Institute (HHMI) Site provides a detailed animation and explanation of translation. The Scitable by Nature Education Site provides great reading material that explains translation. The site gives analogies, examples, and graphic visuals of translation. The McGraw-Hill Site provides an animation of protein synthesis (translation) along with quizzes to test student understanding of translation. Mutated Protein: The Genetic Home Reference Site provides an explanation of the different kinds of mutations that occur in DNA, RNA, and protein. The Cold Spring Harbor Laboratory Site provides an animation of sickle cell anemia. This condition is a specific example of how a mutated protein can affect human health. The Live Science Site provides an explanation of diabetes. This condition is a specific example of how a mutated protein can affect human health. The Abcam Discover More Site provides a list of important proteins that need to be regulated within humans. The site explains that when these proteins are mutated, over produced, or not produced cancer can begin to form. Protein Virtual Labs: The Virtual Analytical Laboratory Site provides an explanation and animation of a protein lab that measures protein concentration. This type of lab is called a Bradford Assay. The Virtual Amrita Laboratories Universalizing Education Site provides an explanation and animation of a protein lab that detects protein and separates protein by size. This type of lab is called a SDS PAGE Gel. The Howard Hughes Medical Institute provides an explanation and animation of a protein lab that detects a specific protein with the use of highly specific binding antibodies. This type of lab is called an ELISA assay.