Store LENTICULE® samples at -20°C ± 5°C
Food and Environmental Proficiency Testing Unit
Laboratory identification no.
(check):
Please Enter your Lab No.
Dispatch date:
2 November 2015
Final date for return of results:
4 December 2015
Results may be returned by fax, mail or email
Contact details:
The Organisers - FEPTU
Public Health England
61 Colindale Avenue,
London, NW9 5EQ, UK.
Fax: +44 (0) 20 8200 8264
Tel:
+44 (0) 20 8327 7119
e-mail: foodeqa@phe.gov.uk
Legionella PCR Scheme PILOT DISTRIBUTION - Request/Report Form
Distribution No: PILOT
Download the safety data sheet:
Sample numbers: GXA and GXB
www.gov.uk/government/publications/safety-data-sheet-for-lenticules
If you cannot examine any of these samples return your results as ‘Not examined’
Request:
(i)
(ii)
Examine for the presence of legionellae
Quantify legionellae in samples
Results
Isolation of legionellae
(enter detected or not
detected (ND))
Final
Results
Identification
(enter L. pneumophila,
species name or ‘not
pneumophila’)
Quantification
GU L-1
FEPTU815.01
GXA
GXB
Detected
Detected
L.pneumophila detected
Not pneumophila detected
8.2x104
1.5x102
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Laboratory identification no.
(check):
Please Enter your Lab No.
We would be grateful if you would answer the following questions before returning this form to help
us with the scheme development:
Store LENTICULE® samples at -20°C ± 5°C
1. Which standard(s)/guidelines did you refer to for the examination these samples? ____ISO
12869_____________________________________________________________
2. Does your laboratory routinely filter samples? If not, please provide details of your concentration
method(s)
______________________________________________
3. Please give details of primers (e.g. in house / commercial kit)
_________________________________________________
4. Describe your DNA extraction method (including volumes)
______________________________________________
5. What volume of the extracted DNA used in PCR?
_______________________________________
6. Please provide details of master mix and volume used
___________________________________________
7. What are your limit of detection and limit of quantification?
______________________________________________
8. Does your laboratory routinely carry out both detection and quantification tests on samples?
_____________________________________________________________
9. Is your laboratory currently participating in any other legionella PCR scheme?
_____________________________________________________________
10. Please provide any further comment/s that will help FEPTU to ensure the scheme is appropriate
when launched:
__________________________________________________________________________
Authorised by:
FEPTU815.01
Date reported:
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