TENDER CIIT EPC 02 (2015-16) ES 01
DEPARTMENT OF ENVIRONMENT (BIOTECHNOLOGY)
Prices are required on C& F Islamabad Basis
S.No Item Name
Lab Name Description of Items
Qty
01
Autoclave
GE
01
02
CO2
Incubator
SL
Interior Material: Stainless steel, Temperature controlled,
Temperature setting method, temperature display method,
timer, Chamber volume 23 lit minimum, Max operational
pressure: 0.18-0.2 MPa
Dimensions External dimensions (WxDxH)770 x 730 x 905
mmInternal dimensions (WxDxH)643 x 523 x 700
mmVolume230
LNet
weight
(approx)90
kgPerformance Temperature
control
range
and
fluctuationAT +5 ~ +50 0.1Temperature uniformity0.25CO2
control range and fluctuation0 ~ 20 0.15Humidity level and
fluctuation95Control Temperature
sensorThermistorCO2
sensorDual
IRDisplayLCD
touch
screenConstruction Exterior materialPainted steel (rear cover
not painted)Interior materialSS copper alloyedInsulation
materialExtruded polystyreneDHA heating systemYes Outer
door 1 Outer door lockoptionReversible doorYesInner
door 1Shelves 4Max. load per shelf7 kgMax. total load20
kgMax. shelf capacity10 kgAccess port - positionReardiameter30Alarms Power failureRearOut of temperature
settingV-B-RHigh temperatureV-B-ROut of CO2 settingVB-RDoor openV-BElectrical and noise level Power
supply230 VFrequency50 HzNoise level25 dB
Co2 Cylinder & Regulator
03
Shakers
ML



Capacity: 144 Flasks of 25 mL to 6 Flasks of 6000 mL
Temperature Range: 0 to 80C
Speed Range: 20 to 420 RPM (at 60 Hz)
02
01

Real-time PCR system for rapid cycling up to 96 samples.
Applications include absolute and relative quantification,
melting curve analysis, and endpoint genotyping. Precise
Instrument thermal homogeneity and cycling speed, accurate
and reproducible results in a very short time.
optical detection system flexibly detects sequence-dependent
probes, such as hydrolysis probes and sequence-independent
dyes (i.e., SYBR Green I). Multiplex and multicolor
capabilities to permit use of up to four different fluorescent
dyes.
Application and Instrument Software creates a simple
analysis workflow with easy import and export functions,
email notifications after each run, online monitoring
flexibility, and server-based network interfacing.
Features
Plate-based real-time PCR system with 96-well block
Temperature gradient (assay optimization)

Standalone system (integrated PC)

Touchscreen with separate instrument software (free to load
on any PC)

Dynamic range of 10 log
04
GE
qPCR
instrument:
01
TENDER CIIT EPC 02 (2015-16) ES 01
DEPARTMENT OF ENVIRONMENT (BIOTECHNOLOGY)
Prices are required on C& F Islamabad Basis

Sensitivity of one copy

4-plex multicolor

Runtime of less than 1 hour

Applications: Absolute Quantification, Relative
Quantification, Endpoint Genotyping, Melting Curve
Analysis

Intuitive state-of-the-art software

PC analysis software can be loaded free of charge on any PC

Thermal cycling system: Peltier-based, 96-well block ,Max
ramp rate heating: 4.4°C, Average ramp rate cooling: 2.2°C,
Programmable temperature range: 37–98°C Temperature
accuracy :±0.2°C of programmed target temperature TM
uniformity Range (max–min) 0.4°C SD < 0.1°C Gradient
function Yes Gradient operational range 37–98°C Gradient
programmable span (X–Y °C) Max. 20°C gradient range
Excitation High-power broad spectrum LED Measurement
(integration time) Simultaneous data acquisition for all
positions in 10–1000 ms (dynamic mode) Detection CCD
Camera Optical system Fixed fiber optics with four
excitation and four emission filters No moving scanning
elements Cq uniformity Range (max–min) 0.8 SD < 0.2
(enabling resolution of 2-fold concentration differences)

Electrical approvals CE, ICE, UL Reaction volumes 10–50
µl Sample format 96-well plates, 8-tube strips Runtime < 40
min for 3-step 40 cycles PCR Melting measurement
Continuous with 1–25 data points/°C
Operating systems Win 7 + Win XP etc, Data analysis Abs
Quantification Rel Quantification TM Calling Endpoint
Genotyping HRM* Qual Detection* Data export Result
table: export as .txt file Charts: Data and image export Data
import Sample information can be imported from Excel, .csv
or .txt files Failure flagging Automated flagging for critical
controls (e.g., positive control is negative or negative control
is positive) User definable flagging for further criteria (e.g.
standard curve with error greater than x)