Vegetable Breeding Program The vegetable breeding program at OSU develops improved vegetable varieties to meet the needs of gardeners, growers, and processors operating in the unique growing conditions of the Pacific Northwest. The Willamette Valley is the main vegetable growing region in the state, and many cultivars require specific adaptation to thrive in its cool Mediterranean climate. Along regional adaptation, the program also focuses on developing cultivars with improved disease resistance. The program works closely with the Oregon Processed Vegetable Commission (link to: http://horticulture.oregonstate.edu/content/oregon-processed-vegetablecommission-0). The program adapts varieties to organic production systems and focuses on enhancing human nutrition value of many crops to address the needs of the growing number of organic farms and gardeners in the region. Projects (use photos on the intro page to link to each of these Project Pages) a. Green beans The Willamette Valley green bean processing industry is known for the high quality of the Blue Lake variety. The green bean project focuses on developing a cultivar with white mold resistance that has comparable processing qualities to Blue Lake. However, the green bean project at OSU also strives to maintain diverse approaches, germplasm and markets for its green bean varieties. Project Highlights: OSU has worked with The Common Bean Coordinated Agriculture Project or BeanCAP (link: http://www.beancap.org/) to create the bean SNP chip and an association-mapping panel of lines for disease resistance and nutrition traits. OSU also used the BeanCAP association mapping panel to map QTLs for white mold resistance. Recent grad student’s (Christina Hagerty’s) thesis project funded by Seneca (link to: http://www.senecafoods.com/)and utilitzed BeanCAP SNP chip to do QTL mapping (biparental RIL population) for resistance to Fusarium and Aphanomyces root rots OSU is looking at white mold resistance genes in the Scarlet Runner Bean and doing Fine-mapping QTLs as part of the National Sclerotinia Initiative, a collaborative project administered by the USDA Agriculture Research Service. Collaborators: Phil McClean, North Dakota State University, BeanCAP Perry Cregan, USDA – Beltsville, BeanCAP Phil McClean, North Dakota State University, National Sclerotinia Initiative Phil Miklas, Washington State University (USDA), National Sclerotinia Initiative Publications: We will be inserting a few publications out of this project. b. Broccoli The Broccoli breeding project is loosely divided into developing 2 types of varieties: one for the Processing market and one for the Organic market. For the Processing market OSU breed for traits that facilitate mechanical harvest and processing. For the Organic markets OSU breeds for traits that help the variety function in organic farming and generally uses open-pollinated cultivars to allow for seed saving. Publications: We will be inserting a few publications out of this project. c. Snow peas OSU snow pea project focuses on developing fresh market varieties that perform especially well in the Willamette Valley. We look for taller, more indeterminate vines and resistance to viruses, powdery mildew and Fusarium wilt. Publications: We will be inserting a few publications out of this project. d. Tomatoes OSU’s goals in tomato breeding are to improve disease resistance and human nutrition. Late Blight is the main disease problem for tomatoes growers in the Pacific Northwest. Isolates in the Pacific Northwest seem to be overcoming resistance genes, which are successful in the eastern United States. With human nutrition in mind, Dr. Jim Meyers breed and released Indigo Rose (link to: http://extension.oregonstate.edu/gardening/purple-tomato-debuts- %E2%80%98indigo-rose%E2%80%99), a purple tomato that is high in anthocyanins. Anthocyanins are a class of flavonoids that have potential human health benefits. Publications: We will be inserting a few publications out of this project. e. Pumpkin Developing a Golden Delicious pumpkin with zucchini yellows virus resistance. Publications: We will be inserting a few publications out of this project. f. Habanero peppers Developing a Habanero pepper adapted to the Pacific Northwest Publications: We will be inserting a few publications out of this project. g. Northern Organic Vegetable Improvement Collaborative (NOVIC) NOVIC (link to: http://eorganic.info/novic) is a collaborative project that brings attention to the need for and breeds varieties that are adapted to organic production. NOVIC breeding takes place on research stations and on organic farms in a variety of climates through out the nation. In addition to breeding varieties, NOVIC maintains a database of variety performance across environments. One trait OSU focuses on breeding for through NOVIC is season extension and overwintering. Varieties that extend the harvest seasons of crops allow growers a greater ability to continually fill fresh market needs. Highlights: Breeding for broccoli adapted to organic production, which are also open-pollinated varieties Breeding for snap peas that are tall, trellised, indeterminate, have a long harvest period, and have a good resistance package Collaborators: Dr. Michael Mazourek, Cornell University Dr. Erin Silva, University of Wisconsin – Madison Micaela Colley, Organic Seed Alliance – Port Townsend, WA Publications: We will be inserting a few publications out of this project. Releases (Have link to a page or a pdf with the below information on the main page) Common Bean Germplasm Released: Name 83B229 6315 83B282 K0440 Market Class Pinto Pinto Great Northern Great Northern Release Date 1988 1988 1988 1988 Common Bean Variety Released: Name Market Class 'UI 686' 'UI 722' 'UI 906' 'UI 125' 'UI 196' 'UI 537' 'UI 137' 'UI 911' 'UI 228' 'UI 239' 'Black Knight' 'UI 320' 'UI 465' 'UI 259' Cranberry Dark Red Kidney Black Small White Pinto Pink Navy Black Small Red Small Red Black Pinto Great Northern Small Red Release Date 1989 1989 1989 1990 1990 1990 1991 1993 1993 1993 1997 1998 1998 1998 Plant Var. Protection Certificate No. 8900253 8900254 8900255 --9100207 9300202 ---9700355 ---- PVP issue date 12/31/1992 07/31/1992 07/31/1992 03/31/1993 01/01/1997 02/15/2001 Vegetable Cultivars Released Release Date Name Description 'Indigo Rose Tomato: Saladette with high levels of anthocyanin in the fruit. 2011 Pending (201100302) 'Legend' Tomato: Large fruited, early maturing, late blight resistant parthenocarpic slicer. 2000 Not obtained OSU5630 Bush blue lake snap bean: high yielding, improved architecture & pod quality. Dry bean: high yielding disease resistant kablanketi type for Eastern & Southern Africa. 2005 Not obtained 2006 Dry bean: high yielding disease resistant kablanketi type for Eastern & Southern Africa. 2006 Not obtained (released through Sokoine Univ. Agric., Tanzania) Not obtained (released through Sokoine Univ. Agric., Tanzania) Mshindi Pesa PVP Vegetable Cultivars Nearing Release Name Description OSU 6443 BBL green bean: Full sieve, good color and processing characteristics, higher yielding and better architecture than Oregon 91G. PEMV, RCVMV, Powdery Mildew, and Fusarium Wilt resistant. Developed for organic production. Open pollinated population selected for improved sprouting characteristics. Resistance to ZYMV and other cucurbit potyviruses. Developed for processing and culinary seed industry. Stringless Snap and Snow Peas Broccoli Golden Delicious winter squash Cucurbita maxima Release Date PVP 2014 Contemplated 2 – 3 lines in 2013 Contemplated Release as OP in 2015 2 – 3 lines in 2014 Contemplated Contemplated Program Structure (Have link to a page with the below information on the main page) a. Faculty Jim Meyers is the primary vegetable breeding faculty. Link to his webpage: http://horticulture.oregonstate.edu/content/jim-myers b. Staff Profiles Shinji Kawai (Research Assistant): I'm Jim's new research assistant and help logistics on the vegetable farm and greenhouse. I enjoy learning all about vegetables and collaborating with researchers, students and farmers. Joel Davis (Faculty Research Assistant): My work in the vegetable breeding program involves using molecular markers as a tool in snap bean breeding. This includes such methods as genome mapping and quantitative trait loci (QTL) analysis, developing PCR-based markers for specific traits of interest, and implementing marker-assisted selection for introgressing disease resistance and other novel traits. I provide technical support in the lab by training students and researchers in molecular techniques, maintaining lab equipment and supplies, and overseeing laboratory safety practices. Other duties include various greenhouse experiments such as pollination and disease inoculation assays, and occasional field work (planting, harvesting, data collection). c. Graduate Students Graduate Student Training Graduate students in the vegetable breeding program train in all aspects of production from planting to harvest, including some processing and running farm machinery. Training is in classic plant breeding and students obtain solid experience in molecular techniques. Students can expect to get extensive experience or knowledge of QTL mapping, marker-assisted selection, association mapping, and the integration of genomics and bioinformatics to identify candidate genes for traits. Current Graduate Students Lyle Wallace I will be mapping flavor traits in snap beans with the goal of improving the flavor of bush blue lake snap beans. Initial research will include panels of tasters who will assess more than a hundred accessions of snap bean and gas chromatography to identify volatile substances associated with flavor traits. Kara Young My research is focused on breeding for disease resistance in tomato. Specifically, I am working on breeding for late blight resistance in tomato. The goal of the project is to develop a tomato variety with durable resistance to late blight. The project involves work to introgress known sources of genetic resistance to late blight into cultivated tomato as well as screening material for novel sources of resistance. Noor Al Bader Haidar Anwar Mustafa Al Jadi Past Graduate Students Students Name Thesis Title Occupation after Completion Christina Hagerty* *NOTE: Title of defense seminar. Thesis not online, so will have to contact graduate Mapping quantitative trait loci for root rot resistance, root traits, and morphological traits in a common bean recombinant inbred population (MS, 2013) Ph.D. candidate, cereal pathology program, Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR Laurie McKenzie* Breeding an open pollinated broccoli for organic production systems using participatory methods (MS, 2013) Transfer, characterization and mapping of white mold resistance in an advanced backcross interspecific population between Phaseolus vulgaris and Phaseolus coccineus (MS, 2011) Pyramiding quantitative trait loci conditioning partial resistance to Sclerotinia sclerotiorum in bush blue lake green beans (Phaseolus vulgaris) (MS, 2010) Breeding tomato for increased fruit phenolics (PhD, 2009) Characterization of physiological resistance to white mold and search for molecular markers linked to resistance via advanced backcross QTL analysis in an interspecific cross Breeding Internship, Organic Seed Alliance, Port Townsend, WA *NOTE: Title of defense seminar. Thesis not online, so will have to contact graduate Shawna Zimmerman Miles Barrett Peter Boches Erron Haggard MS Student, Graduate Degree Program in Ecology, Colorado State University, CO Research Technician , Barenbrug USA, Tangent, OR Blueberry Breeder, Fall Creek Nursery, Lowell, OR Cucurbit Breeder, Hollar Seed Co., Rocky Ford, CO Paul Kusolwa between Phaseolus coccineus and P. vulgaris (MS, 2007) Breeding for bruchid resistance in common bean (Phaseolus vulgaris L ) : interspecific introgression of lectin-like seed proteins from tepary bean (P. acutifolius Gray), genetic control and bruchid resistance characterization (PhD, 2008) Lecturer, Sokoine University of Agriculture, Morogoro, Tanzania Barbara Gilmore Genetic resistance to white mold (Sclerotinia sclerotiorum (Lib.) De Bary) in scarlet runner beans (Phaseolus coccineus L. ) (PhD, 2007) Nursery Care, One Green World, Molalla, OR Peter Mes Breeding tomatoes for improved antioxidant activity (PhD, 2005) Traditional and molecular approaches to Zucchini yellow mosaic virus resistance in Cucurbita (PhD, 2001) Vegetable Breeder, AgroGene, Twin Falls, ID Carl Jones Not online – will have to check hard copies Nihat Guner Not online – will have to check hard copies Kevin McPhee Not online – will have to check hard copies Vegetable Trait Integration Strategy Lead, SeminisMonsanto, Sacramento, CA Global R&D Head Watermelon & Senior Watermelon Breeder, Sakata Seed America Inc., Ft. Myers, FL Assistant Professor & Pulse crops breeder, Department of Plant Sciences, North Dakota State University, Fargo, ND Muhammad Shahsawar Not online – will have to Rebecca Brown Assistant Professor of Plant Breeding, Department of Plant Sciences and Entomology, University of Rhode Island, Kingston, RI check hard copies d. Lab infrastructure: (is this needed?) On campus: Molecular lab Pathogen culture facilities Wet lab (for seed work, primarily) Greenhouses On farm (OSU vegetable farm): Extensive field space Seed storage Wet lab Processing equipment