0006 Native revegetation of previously cultivated land in the dry mixedgrass natural subregion of Alberta, Canada. Jennifer Richman1, Brad Downey2, Kevin France1, Paul Jones2, Brandy Downey1, Francois Blouin3 1Alberta Environment and Sustainable Resource Development, Lethbridge, Alberta, Canada, 2Alberta Conservation Association, Lethbridge, Alberta, Canada, 3Prairie Conservation Forum, Lethbridge, Alberta, Canada MULTISAR is a multiple species conservation strategy that aims to conserve multiple species of wildlife, including Species at Risk, at the landscape level, through a process that integrates range management with fish and wildlife management principles. In the early 1900's a significant amount of native dry Mixedgrass range was ploughed to make way for intensive agriculture. Currently, only 43% of the dry mixedgrass natural subregion remains native and continues to be fragmented by human development. In 2008, the MULTISAR program partnered with a landowner to convert 57ha of marginal cropland back to native grassland. A seed mix for the site was determined by assessing the ecological characteristics of the native grasslands immediately adjacent and identifying the composition of the plant community. Seed was broadcast at 10kg/ha followed by a light harrow in the spring of 2008. In the spring of 2009 the site was sprayed with a broadleaf herbicide to combat weeds and 150 silver sagebrush plugs were planted in low lying areas. In 2010 after the third growing season, species composition equaled 13.4% blue grama (Boutelua gracilis), 13% northern wheatgrass (Agropyron dasystachyum), 10.9% June grass (Koeleria macrantha), 6.7% western wheatgrass (Agropyron smithii), and 4.7% needle and thread grass (Stipa comata). Wildlife present on the site shifted from a community dominated by horned larks (Eremophila alpestris) in 2007, to thirteen species identified in 2010 including species at risk like Sprague's pipit and chestnut-collared longspur. Further research is needed to determine their nesting and rearing success on reseeded native grasslands. 0015 Perennial Grass Establishment Following Cheatgrass Control Using Herbicides Charlie D Clements, Daniel N Harmon USDA-ARS, Reno, NV, USA The introduction and subsequent invasion of Cheatgrass (Bromus tectorum) onto Intermountain rangelands has resulted in increased frequencies of wildfires and severely altered native plant communities. The objective of this study was to test the effectiveness of herbicides [Imazapic (Plateau), Rimsulfuron (Matrix), Sulfometuron Methyl (Landmark)] on controlling cheatgrass and allowing for the establishment of seeded species. Twelve plots, 25m x 50m, were established in fall 2010 and treated with 1) Imazapic @ 6oz/ac, 2) Rimsulfuron @ 4oz/ac, and 3) Sulfometuron Methyl @ 1.75oz/ac rates and replicated 3 times in a completely randomized block design with controls. The treated plots were fallowed for one year and then seeded to Siberian wheatgrass (Agropyron fragilla ssp. sibiricum) at 7 lbs/ac rate. Sulfometuron Methyl yielded the highest control of cheatgrass above ground densities from 24.7/ft² down to 0.4/ft² (98.7%) followed by Imazapic, 17.6/ft² down to 0.8/ft² (95.6%) and Rimsulfuron 13.1/ft² down to 1.1/ft² (91.9%). The control plots averaged 39 cheatgrass plants/ft². Siberian wheatgrass seedlings emerged in March 2012 at fairly high densities (22.5/ft² in the Sulfometuron Methyl treated plots) and by mid-June 2012 still yielded excellent results in some of the treatments. Sulfometuron Methyl treated plots yielded 6.5/ft², followed by Imazapic, 4.1/ft² and Rimsulfuron, 1.5/ft². Control plots yielded 1.1/ft² despite only receiving 6.2" of annual precipitation. These numbers are expected to decline over the hot dry summer months, yet the Sulfometuron Methyl and Imazapic treated plots should result in sufficient long-lived perennial grass establishment to suppress cheatgrass densities in the near future. 0016 Combustion dynamics of cattle fecal pats John Derek Scasta, Dave M. Engle, John R. Weir Oklahoma State University, Stillwater, OK, USA The combustion of cattle fecal pats could have implications on focal micro-disturbances and production issues associated with cattle parasites. In 2011 and 2012, we geospatially identified fifty cow pats in six pastures (n = 300) and assessed combustion of each pat in relation to multiple variables, including: fire weather (relative humidity, temperature, hours since precipitation); fecal pat characteristic (index of age, volume); four time-lag fuel classes (1 hr, 10 hr, 100 hr and 1000 hr) and fuel load (at each pat). Multiple linear regression using AIC was applied to determine the best combustion models, and stepwise selection was used to select the best multiple regression model. Combustion of pats ranged from 3% to 62%. None of the fire weather variables were significant. Of the fecal pat characteristics, only the age score index was significant, and of the fuel variables, only 100-hr fuel moisture and fuel load were significant. The suggested regression model (r2 = 0.4266; p < 0.0001) was comprised of a three way interaction (100-hr fuel moisture × age score index × fuel load). Simple linear regression of pat combustion averaged for each pasture (n = 6) and 100-hr fuel moisture was highly correlated (r2 = 0.9587). These data suggest that grazing and fuel characteristics could be managed to optimize combustion of pats for a variety of ecological or production oriented goals. 0017 Perceptions of Drought: How Ranchers in Arizona Prepare for and Respond to Drought Greg Butler, George Ruyle, Mitch McClaran, Diane Austin University of Arizona, Tucson, Arizona, USA Ranchers and land managers in Arizona must respond quickly to drought in order to employ the most effective management practices for maintaining forage utilization without degrading livestock or range conditions. I determined relationships between rancher perceptions of drought, practices used in preparation for and response to drought, drought preparedness and sources of drought management information. In order to evaluate these relationships, I sent a mail survey to ranchers and land managers in eastern and southeastern Arizona. Respondents consider the most important practices used both to prepare for and respond to drought to be rotational grazing, reducing herd size, adding more drinking water sources and purchasing additional feed. Sixty eight percent of respondents believe they are more prepared for drought now than they were 10 years ago in spite of worsening drought conditions. They understand they cannot control the weather, but they can control how they manage their operation in response to changing weather conditions. 0036 Development of South Texas Germplasm sideoats grama and comparison to previous releases Keith Pawelek1, Forrest Smith1, Anthony Falk1, John Lloyd-Reilley2, Shelly Maher2, William Ocumpaugh1 1South Texas Natives, Caesar Kleberg Wildlife Research Institute, Texas A&M University-Kingsville, Kingsville, TX, USA, 2United States Department of Agriculture (USDA), Natural Resources Conservation Service (NRCS), E. “Kika” de la Garza Plant Materials Center, Kingsville, TX, USA Sideoats grama is a widespread native bunchgrass in North America, which is often used in reclamation plantings. Eleven seed releases of Bouteloua curtipendula have been made through the work of the USDA NRCS Plant Materials Program and their collaborators. In addition to these tested materials, a number of wild-harvested seed sources of sideoats grama are offered for sale by commercial seed companies, however, no regionally adapted or locally originating (e.g. ecotypic) seed source of sideoats grama has been available for restoration projects in the region known as south Texas. As a result, we began work to collect, evaluate, and develop a seed release of a blend of ecotypic populations for use in the area. South Texas Germplasm sideoats grama (Bouteloua curtipendula [Michx.] Torr. Var. caespitosa Gould&Kapadia) was cooperatively released in 2012 as a Texas Selected Native Plant Germplasm. This germplasm is a blend of six selected native populations originating from the Rio Grande Plains and Edwards Plateau Ecoregions of south Texas. Following initial evaluation, selection, and isolated seed increase of each component, South Texas Germplasm was compared to sideoats grama cultivars originating from nearest the area of intended use of the germplasm: ‘Haskell', ‘Premier' and ‘Vaughn', in a 6-site variety trial utilizing transplanted and seeded experimental plots. South Texas Germplasm exhibited significantly higher 90-day seedling emergence than available cultivars at planting sites within the area of intended use, as well as superior performance in the transplant experiment at 2 of 3 experimental sites in south Texas. 0038 Who can take the heat: Caespitose grass response to increasing fuel loads Ryan Limb Oregon State University, Corvallis, Oregon, USA Fire is a natural disturbance that frequents most landscapes globally and produces varying disturbance intensities depending on several factors. Likely the most easily understood and manipulated factor affecting fire intensity on rangelands is the herbaceous fuel load. Alterations in plant community composition and structure in addition to decreased grazing intensities have led to relatively high fuel loads on many western North America caespitose grass rangelands. Increased fuel loads directly increase fire intensity, but also increase other factors that may be more critical to plant survival including residual flame time, burn duration and total heat transfer. Numerous reports suggest that increased fire intensity reduces bunchgrass survival, but often these studies were conducted in wildfire situations where exact fuel loads and fire conditions were not knows. We planted four bunchgrasses native to western North America (Achnatherum thurberianum, Elymus elymoides, Festuca idahoensis, Psuedoroegneria spicata) in 2.5 L pots and maintained them in a climate controlled greenhouse. At 20 weeks old, we burned individual plants under controlled conditions with herbaceous fuel loads ranging between 2000 kg∙ha-1 to 4000 kg∙ha-1. An additional cohort of plants was clipped to 2-cm height to simulate a grazing event. We compared survival of individual plants among the different fuel load and clipping treatments and a nontreated control 21 days post-treatment and tracked the length of time to produce green biomass among the surviving individuals. 0039 Impact of High Stocking Density Grazing on Livestock Walking and Resting Behavior Alexander Smart, George Perry, Julie Walker, Cody Wright, Megan Mortellaro, Kevin Vander Wal SDSU, Brookings, SD, USA High stocking density "mob" grazing is a recent phenomenon that is being promoted by a few practitioners, but has not undergone rigorous scientific testing. The objective of this study was to determine the walking and resting behavior of cattle grazing at high and low stocking densities. The study was conducted in 2012 on smooth bromegrass (Bromus inermus L.) pasture near Brookings, South Dakota. Two replicate treatments consisting of livestock grazing at a high stocking density (200,000 kg of live weight per ha moved three times per day) or a low stocking density (3,600 kg of live weight per ha moved once per week) were compared. Pedometers were put on three heifers for each replication of the high stocking density treatment and two heifers in each replication of the low stocking density treatment. Pedometers recorded number of steps, the number of laying bouts, and time laying down in one second intervals. Number of steps taken per day in the high stocking density treatment (3103 steps) was 40% more (P=0.02) than the low stocking density treatment (2218 steps). Daily laying bouts was 50% fewer (P=0.01) for high stocking density (10) than for low stocking density (15). Daily resting time was 10.5 hours for heifers in the high stocking density treatment compared with 11.1 hours in the low stocking density treatment (P=0.03). It would appear that high stocking density grazing might cause cattle to do more walking and expend more energy compared with low stocking density rotational grazing. 0054 Long-term alfalfa and clover recovery dynamics in northern temperate pastures sprayed with residual broadleaf herbicide. Amanda Miller1, Edward Bork1, Linda Hall1, Patrick Forsythe2, Bill Summers0 1University of Alberta, Edmonton, Alberta, Canada, 2DuPont, Wetaskawin, Alberta, Canada Beneficial legumes such as alfalfa (Medicago sativa) and white clover (Trifolium repens) increase the productivity of pastures and quality of forage, and are important features of northern temperate pastures. When pastures are sprayed for the control of broadleaf weeds existing legumes are removed from the plant community. Although prompt re-establishment of legumes in sprayed pastures is a goal of many land managers, this process depends on a number of different factors. Current re-cropping guidelines for many herbicide bioactives are based on data from areas with different soil and climatic conditions relative to western Canada. This study used long-term plots to track the degradation of herbicide bioactives (aminopyralid and aminocyclopyrachlor) via the performance of key forage legumes: alfalfa and white clover. Five long-term field sites were established to assess the ongoing recovery of legumes by investigating changes in legume biomass for 3 years following herbicide application. Each experimental site was organized into 4 replicate blocks of a randomized strip-split plot design. Mowing main plots were randomly assigned to ½ of each block, herbicide subplots assigned to each ¼ plot, and seeding subsubplots (alfalfa, clover, or non-seeded) repeated between the mowed and non-mowed portions of herbicide sub-plots. Herbicide bioactives were applied at two rates (0x or 1x) to herbicide subplots. Legume performance differed modestly between plots sprayed with the two different bioactives, and more markedly between mowing regimes. As re-establishment of legumes is an important goal, this information provides insight into the expected return of legumes under field conditions. 0057 Impact of High Stocking Density Grazing on Litter Decomposition Megan Mortellaro, Alexander Smart, Jiyul Chang, Michelle Ohrtman, Sharon Clay, David Clay South Dakota State University, Brookings, SD, USA High Stocking density “mob” grazing is a recent phenomenon that is being promoted by a few practitioners, but has not undergone rigorous scientific testing. The objective of this study was to determine the decomposition of litter from cattle grazing at high and low stocking densities. The study was conducted in 2011-2012 on smooth bromegrass (Bromus inermus L.) pasture near Brookings, South Dakota. Two replicate treatments consisting livestock grazing at a high stocking density (200,000 kg of live weight per ha moved two times per day) or a low stocking density (5,800 kg of live weight per ha moved once per week) for 2consecutive years were compared. A 2x2x2x2 factorial treatment design was used to examine litter decomposition by stocking density treatment (high or low), liter type (fresh or old), placement (surface or buried at 7.5 cm), and origin (high or low stocking litter) in-situ from August 2011 until mid-May 2012. After collection, samples were washed, dried and weighed. Fresh litter decomposed 17% more than old litter (55% vs. 47%; P<0.01). Buried litter decomposed 55% more than surface litter (62% vs. 40%; P<0.01). There was no difference in decomposition between surface samples in high (39%) and low (41%) stocking densities, but significantly more decomposition occurred in buried samples at high (66%) than low stocking densities (58%; P=0.01). Results indicate that high stocking densities cause a higher rate of decomposition and greater nutrient cycling below the soil surface. 0058 Cheatgrass percent cover and dieoff mapping in the northern Great Basin, USA Stephen Boyte1, Bruce Wylie2, Donald Major3, Matthew Rigge4 1Stinger Ghaffarian Technologies, Inc. Contractor to the U.S. Geological Survey EROS Center, work performed under contract G10PC00044, Sioux Falls, SD/Midwest, USA, 2U.S. Geological Survey, EROS Center, Sioux Falls, SD/Midwest, USA, 3BLM Idaho—Great Basin Restoration Initiative, Bosie, ID/West, USA, 4Arctic Slope Regional Corporation Research and Technology Solutions, contractor to U.S. Geological Survey, EROS Center, work performed under contract G08PC91508., Sioux Falls,SD/Midwest, USA The Great Basin contains endangered ecosystems, and cheatgrass (Bromus tectorum) is a significant driver of change in this region. The presence of this invasive plant in shrub-steppe ecosystems results in increased fire frequency, size, and severity, and it now dominates large portions of the ecosystems, degrading the landscape. The conversion from diverse ecosystems to ones dominated by a single species reduces habitat for wildlife and forage for livestock. In recent decades, cheatgrass dieoff occurred across relatively large expanses of this region. We defined cheatgrass dieoff as stand failure causing the absence, or reduced production, of cheatgrass during years of adequate precipitation in previously invaded areas. Cheatgrass dieoff might be perceived as beneficial, but dieoff causes accelerated soil erosion, loss of early spring forage, and unknown recovery pathways. To better understand cheatgrass dynamics on a landscape scale and to provide science-based information on dieoff magnitude and trends, we developed and analyzed a time series of cheatgrass percent cover and dieoff maps by integrating satellite data into ecological models. When integrated with research that examines causes and consequences of dieoff, our work can facilitate cheatgrass management, fuels management and fire suppression strategies, and conservation/restoration planning. The percent cover model had an R 2 = 0.85 and the dieoff model had an R2 = 0.88. We also developed a long-term cheatgrass percent cover model (cheatgrass site potential) and a cheatgrass dieoff probability model (accuracy = 88%), both of which are flexible and which allow input of future climate data to estimate future cheatgrass dynamics. 0059 Seedling Success from Germination to First Year Mortality Event: Cheatgrass Versus Three Perennial Grass Species Dan Harmon, Charlie D Clements USDA-ARS, Reno, NV, USA Revegetation of Great Basin arid rangelands is an expensive yet largely unsuccessful process. Establishment predominantly falls below requirements for cheatgrass (Bromus tectorum) suppression (~10 plants/m2). Recent seedling demographic research suggests that seedling mortality from drought and lack of germination are not the main cause of failure but inability to emerge after germination. We examined germination, emergence and seedling survival of cheatgrass, squirreltail (Elymus elymoides), bluebunch wheatgrass (Pseudoroegneria spicata) and ‘hycrest' crested wheatgrass (Agropyron cristatum) seeded (October 2011) in multiple soil types at the Reno ARS research location. Cheatgrass germination occurred by February (temperature low=27F/-3C: high=37F/3C, 12% gravimetric moisture) and perennials by March (Low=33F/1C: high=41F/5C, 10% gravimetric moisture). Maximum emergence occurred by April 18 (temperature low-=40F/4C: high=59F/15C). April germination percent yielded: cheatgrass 27% (range=47-11), bluebunch wheatgrass 23% (range=45-8), ‘hycrest' wheatgrass 22% (range=30-10), and squirreltail 14% (range=30-6). Emergence resulted in: cheatgrass 17% (37% decrease post germination), bluebunch wheatgrass 17% (26% decrease), ‘hycrest' 18% (23% decrease) and squirreltail 15% (7% increase). Seedling survival by late May was: cheatgrass 2.3% (87% decrease), bluebunch 1.25% (93% decrease), ‘hycrest' 2.55% (86% decrease) and squirreltail 2.15% (86% decrease). July success per seeding effort was: cheatgrass 3.3%, "hycrest' 2.5%, Squirreltail 1.3% and bluebunch 1.1%. We conclude that seedling mortality from drought is the largest contributor to seedling failure (88%) comparable with germination (78%). Emergence contributed 20% failure. We recognize potential effects of drought (Reno precipitation [October-May] normal 5.4" vs. 2011-2012 actual 2.6"). Results however concur with our years of wild land seeding experiences and observation of seedling mortality. 0060 The fitness costs of delayed germination and diminutive growth response of cheatgrass Dan Harmon, Charlie D Clements USDA-ARS, Reno, NV, USA The competitive ability of cheatgrass is often attributed to rapid early season germination. Our previous research has observed germination occurring from October through June near the Reno Nevada ARS research location. In a controlled experiment we allowed cheatgrass to germinate naturally (October 2010) and then later in March (2011). Maturation date and biomass were then compared. Plants that had delayed germination matured later (~ 4weeks), had significantly smaller biomass (March germination=0.48g, October germination=8.48g), and had a greater percent occurrence of seed smut (March germination=22%, October germination=3%). These results indicate a large fitness cost of delayed germination. To further examine the cost of delayed germination we conducted an experiment with intraspecific competition. We previously found that in greenhouse competition experiments if native annuals were allowed to germinate four weeks prior to cheatgrass germination (an anomaly in nature), cheatgrass has very diminutive growth (biomass =0.57g after 24 weeks growth compared to 5g without competition). In 2012 we conducted an experiment to determine these results with intraspecific competition. We again found that if a target cheatgrass plant was started in a pot a few weeks after other competing cheatgrass seedlings germinate it had very diminutive growth, indicating resource depletion by the earlier germinating competitors. Soil nutrition analysis however did not find a drastic decrease in nutrients below the growth potential of cheatgrass. This possibly indicates a growth response mechanism triggered by earlier germinating plants occupying space, either above ground or in root zones. 0064 Defoliation and moisture effects on Dry Mixedgrass Prairie diversity, richness, evenness, and composition Tanner Broadbent1, Edward Bork1, Walter Willms2 ,1, Janice Cooke1 1University of Alberta, Edmonton, Canada, 2Agriculture and Agri-Food Canada, Lethbridge, Canada With growing impetus for rotational grazing on semiarid rangelands, it is necessary to understand the effects of defoliation regimes of varying intensities and frequencies on plant community diversity and composition. Moreover, uncertainty surrounds grazing effects on semiarid plant communities with indeterminate dominance of above- (i.e., space and light) and below-ground constraints (i.e., water and nutrients) on plant growth, such as the Dry Mixedgrass Prairie, with both canopy dominant mid-grasses coexisting with shorter species. We assessed plant foliar cover at three intervals (early, middle, and late in the growing season) in plots under factorial treatments of high intensity-low frequency (HILF), high intensity-high frequency (HIHF), low intensity-high frequency (LIHF), and control (deferred) defoliation in combination with either ambient moisture and moisture addition in a fully randomized design at two sites in the Dry Mixedgrass Prairie, including a xeric sandy site and mesic loamy site. Species richness, evenness, and diversity (Shannon’s index) were assessed using repeated measures ANOVAs. Defoliation effects on diversity and evenness varied between sites while moisture alone had no effect, and all parameters peaked early in the growing season. All defoliation regimes promoted diversity and richness in the loamy site, whereas only HILF defoliation under moisture addition promoted diversity in the sandy site. Overall, species evenness among plots was markedly greatly under HILF defoliation. Results suggest that defoliation regimes common to rotational grazing can promote plant community diversity, richness, and evenness in mixedgrass prairie plant communities, but these responses depend on edaphic conditions, such as soil texture and moisture. 0068 Alberta ESRD Rangeland Plant Community Classification System Craig DeMaere, Mike Alexander Range Resource Mangement Program, Pincher Creek, AB, Canada In the mid 1980's Alberta's public rangeland managers identified a need for a more detailed inventory of biophysical and productivity potentials of their land base. The historic zonal rates used to set livestock stocking levels had been deemed inadequate to achieve proper management. Since that time much work has been completed in creating a classification system for rangelands that captures enough detail to facilitate management. This work has led to the creation of range plant community guides. These guides utilize statistical techniques that group similar field plots together within a hierarchal framework. Once a plant community is established it is described by plant species composition and common physical characteristics (soil, slope, aspect). An Ecologically Sustainable Stocking Rate is also calculated based on production estimates and historic field knowledge. Each individual pasture or distribution unit is inventoried by these established plant communities. Currently inventories are carried out on most public land grazing dispositions in Alberta. Once completed, these inventories provide a carrying capacity for the disposition. Carrying capacity values are adjusted at the polygon and field unit level to compensate for access, management and animal distribution to achieve a sustainable grazing capacity. Other benefits from our classification work include: developing an understanding of successional pathways between plant communities within the same ecological site, creating a basis of measure for rangeland health assessments, and providing a measuring stick for reclamation efforts. This classification system has also been utilized as a framework for multi-discipline landscape level inventories. 0072 Effects of fire and herbivory on an endangered terrestrial orchid in a Texas post oak savanna William Rogers1, Carissa Wonkka1, Dirac Twidwell0 ,2, Fred Smeins1 1Texas A&M University, College Station, Texas, USA, 2Oklahoma State University, Stillwater, Oklahoma, USA Fire and herbivory are widely recognized as important in savanna ecosystems, however, little is known about their effects on Spiranthes parksii, an endangered terrestrial orchid endemic to Texas post oak savannas. We designed in a full-factorial, completely randomized field experiment to assess fire and vertebrate herbivory on S. parksii. Thirty-two 1.5x1.5m plots were established in areas of high orchid abundance. Treatments included i) burned and fenced, ii) burned, not fenced, iii) fenced, not burned, and iv) unburned, unfenced. Pre-treatment data were collected and 2-m tall fences with 10-cm wire mesh were erected in May 2009. Fires were conducted in July 2010 using a steel burn-box. We routinely collected data on orchids, herbaceous cover, light, and soil moisture in each plot. Excluding large herbivores did not have an effect on rosettes, but flowering stalk numbers were significantly higher in fenced plots in 2010 (p=0.008) and 2011 (p=0.03). S. parksii rosettes in burned plots had greater leaf area in 2010, but this trend reversed in 2011 (p<0.05). In 2011-2012, both rosette emergence (p=0.03) and senescence (p=0.03) occurred later in burned plots. In 2010, unburned plots averaged one flowing stalk per plot, whereas only one orchid flowered overall in the burned plots (p=0.01). Flowering stalk herbivory was similar in burned and unburned plots, however with very few stalks emerging, it is difficult to interpret whether fire affected herbivore activity. No flowering stalks emerged in 2011, but this was likely due to historically extreme drought conditions throughout much of Texas prior to flowering season. 0073 A Unique Partnership In Rangeland Stewardship and Management:Alberta Environment and Sustainable Resource Development and the Rocky Mountain Forest Range Association Michael Alexander1, Dan Smith2, Roxy Wideman3 1Alberta Environment and Sustainble Resource Development, Pincher Creek, Alberta, Canada, 2Alberta Environment and Sustainable Resource Development, Edmonton, Alberta, Canada, 3Rocky Mountain Forest Range Association, Nanton, Alberta, Canada In 1998 Forest Reserve grazing permit holders and the Alberta Government entered into a unique partnership, through a memorandum of understanding, with the goal of improving management and stewardship of the Rocky Mountain Forest Reserve grazing allotments in southwest Alberta Canada. Permit holders formed an association where membership is voluntary. The Rocky Mountain Forest Range Association (RMFRA) collects a membership fee of $1.00/ AUM from Forest Reserve permit holders. Current membership in the RMFRA exceeds 95% of the 259 Forest Reserve grazing permit holders. The RMFRA works closely with Alberta Environment and Sustainable Resource Development to use its membership fees and any additional funds they can access to achieve their mandate: To ensure the sustainability of livestock grazing in the Rocky Mountain Forest Reserve. Since inception, the RMFRA has funded over $400,000 of range inventory work and over $850,000 in total investment towards enhanced range stewardship, management and knowledge development. The knowledge gained from RMFRA initiatives has improved the management of rangelands in the Forest Reserve, and improved integration of grazing with other resource users. Recently the RMFRA has expanded their program to include other resource management issues through research projects, grazing trials and policy development on such topics as livestock behaviour, riparian management, prescription grazing and grazing-timber integration. For their outstanding achievements and contributions to range management, the Society for Range Management awarded the Rocky Mountain Forest Range Association with a 2012 Outstanding Achievement Award. 0079 Restoring the Sagebrush Component to Crested Wheatgrass-Dominated Communities Kirk Davies1, Chad Boyd1, Aleta Nafus2 1USDA-ARS, Burns, OR, USA, 2Oregon State University, Burns, OR, USA Monotypic stands of crested wheatgrass, an introduced grass, occupy vast expanses of the sagebrush steppe. Efforts to improve habitat for sagebrush-associated wildlife by establishing a diverse community of native vegetation in crested wheatgrass stands have largely failed. Instead of concentrating on a diversity of species, we evaluated the potential to restore the foundation species, Wyoming big sagebrush in crested wheatgrass stands. We investigated the establishment of Wyoming big sagebrush into six crested wheatgrass stands (sites) by broadcast seeding and planting seedling sagebrush across varying levels of crested wheatgrass control with glyphosate. Planted sagebrush seedlings established at high rates (~70%), even without crested wheatgrass control. However, most broadcast sagebrush seeding failed. Only at high levels of crested wheatgrass control did a few sagebrush plants established from seed at a couple of the sites. Subsequently, sagebrush density and cover were much greater with planting seedlings than broadcasting seeding. Sagebrush cover, height, and canopy area were greater at higher levels of crested wheatgrass control. High levels of crested wheatgrass control also created an opportunity for exotic annuals to increase. Crested wheatgrass was rapidly recovering from control treatments; suggesting multiple treatments may be needed to effectively control it. Our results suggest that planting sagebrush seedlings can structurally and functionally diversify monotypic crested wheatgrass stands to provide habitat for sagebrush-associated wildlife. Though this is not the full diversity of native functional groups that previously occupied crested wheatgrass stands, it is a substantial improvement over other efforts that have largely failed to alter these plant communities. 0081 Distribution differences of Larrea tridentata seed density in relation to Ambrosia dumosa and Ambrosia deltoidea canopy cover Ashley Shepherd, Jeffrey Fehmi University of Arizona, Tucson, AZ, USA Larrea tridentata (Creosote bush) is a long lived shrub widespread throughout deserts of the southwest. Understanding germination and seedling survival patterns is crucial since a variety of wildlife species use L. tridentata as a den or to avoid high summer temperatures and predators. Ambrosia dumosa (white bursage) and A. deltoidea (triangle-leaf bursage) exhibit plant-protégé interactions with L. tridentata. Seed density of L. tridentata was measured under both Ambrosia species to determine the role of seed distribution and its effects on the nurse plant-protégé relationship. Further statistical analysis of seed density concluded density was greater under both Ambrosia species canopy (live and dead) compared to areas with no canopy cover, with 0.0326 seeds/cm 3 and 0.0048 seeds/cm 3 respectively (p=0.037). Canopy type (dead or live) did not affect seed density as it only differed by 0.0016 seeds/cm 3 (p=0.421). An absence of L. tridentata seedlings was observed possibly due to specific germination requirements, soil crusting, seed/seedling predation, but not the absence of seed. 0084 Effects of Gap Disturbance on the Seedling Emergence, Survival and Growth of Two Different Native Species in China Haiming Kan, Yingjun Zhang, Nan Liu China Agricultural University, Beijing, China Grassland restoration is an urgent issue in China. Reintroduction of native species has proven to be a highly effective way for restoration of degraded grassland. A field study was conducted to investigate the effects of gap disturbance on the process of two native species seedling establishment. Seeds of Agropyron cristatum and Stipa krylovii were reseeded to artificially created gaps in a degraded steppe in North China. There were seven treatments with each of eight replications: shoot gaps and shoot + root gaps (10cm, 20cm and 40cm in dia.), no gaps (control). Shoot gaps were formed by removing aboveground vegetation and belowground biomass without restricting the re-growth of neighbor roots back into the gap, and then the shoot + root gaps were accomplished by using PVC pipe driven in the soil of shoot gaps. Survival and growth performance after 90 days of seed sowing were recorded for both species. Gap disturbance significantly increased the soil moisture, especially for shoot + root gaps. Seedling emergence and survivorship of both species were greater in gaps than in controls. However, the gap size showed a significant negative effect to Agropyron cristatum's survivorship. Growth performance of Agropyron cristatum and Stipa krylovii differ in their response to gap disturbance. Gap-treatments had positive effects on seedling growth (include seedling height, dry weight, and numbers of tillers and leaves) of Stipa krylovii, but contrary of Agropyron cristatum. 0086 The Internet, Innovation, and the Range: Documenting Ranchers' Internet Use and Preferences Shayan Ghajar, Maria Fernandez-Gimenez Colorado State University, Fort Collins, CO, USA The distribution and speed of internet access continue to improve, offering unprecedented ability to both disseminate and locate information on rangeland management. Innovation diffusion theory suggests that the internet is both an innovation that continues to diffuse throughout the United States, and a vehicle by which innovations may be communicated with very little transaction cost relative to previous methods of communication. Increasingly, scientists and educators are being encouraged to use the internet as a platform for outreach to land managers such as natural resource professionals and ranchers. However, to date, no study has comprehensively documented the internet use patterns of ranchers, and few scientific studies have examined the internet use patterns of natural resource professionals. As both the quantity and scope of rangeland management information available online continue to grow, documenting and understanding the efficacy of digital outreach to natural resource professionals and ranchers becomes more critical. This poster will present the results of a quantitative and qualitative assessment and evaluation of extant rangeland outreach websites, along with the preliminary results of qualitative semistructured interviews with ranchers, Extension professionals, and webmasters about their use of the internet. 0088 Perennial Grass Biomass is related to Grazing Exclusion and Ecological Site, but not Mesquite Cover Amber Dalke, Mitchel McClaran University of Arizona, Tucson, AZ, USA Mesquite (Prosopis spp.) encroachment is often believed to reduce grass abundance. We evaluated this, accounting for grazing exclusion and ecological site, on perennial grass biomass at the Santa Rita Experimental Range in southern Arizona. Three biomass (g/m2) measures were used: total per plot, total covered with mesquite (Under), and total not covered by mesquite. Biomass was measured in paired grazed and long-term (1917) exclosures within three ecological sites (12-16” precipitation): Loamy Upland (LU), Sandy Loam Upland (SLU), and Sandy Loam Deep (SLD). Mesquite cover ranged from 0-40%. It did not differ between grazing and exclusion or SLD and SLU, but was less in LU. Regressions between biomass and mesquite cover were limited to SLU and SLD. There were few significant relationships, all with low adjusted R-squared values suggesting biomass is unrelated to mesquite cover. Biomass was related to ecological site and grazing exclusion using two-way ANOVA. Ungrazed areas had more biomass, mainly from natives, despite low-moderate stocking rates since 1970s. There was more native Under without grazing, but more introduced Under with grazing, suggesting introduced grass responds better to defoliation under mesquite than natives. Native and introduced biomass (including Lehmann lovegrass, Eragrostis lehmanniana) was greater in LU than SLU and SLD suggesting more clay results in more plant production. Plains bristlegrass (Setaria macrostachya) had an interaction indicating LU ungrazed had more biomass than all other grazing practices and ecological sites. Perennial grass biomass was unrelated to mesquite cover between 0-40%, but was related to grazing exclusion and ecological site. 0091 CHANGES IN INTAKE AND NUTRITIONAL QUALITY OF SALT CEDAR Colt Knight1, Cody Scott1, Corey Owens1, Travis Whitney2 1Angelo State University, San Angelo, TX, USA, 2Texas Agrilife Research and Extension Center, San Angelo, TX, USA Recently weaned Boer-cross (n=20) and Angora (n=6) goats were individually penned and fed increasing amounts of salt cedar (Tamarix ramosissima Ledeb.) at weaning. For the first 14 d of trial, goats were fed 2.0% BW of a basal diet. Following the first 14 d, goats were then fed the basal diet at 1.5%, 1.0%, 0.5%, and 0.0% BW for 7 d sequentially in addition to salt cedar. Freshly harvested salt cedar was fed for 30 min each day prior to feeding the basal diet. The amount of salt cedar offered each day was increased as intake increased. Weight, intake, and water consumption data were monitored. In addition, salt cedar samples were collected during the months of June, July, August, September and October and analyzed for in vitro digestibility. Boer-cross and Angora goats consumed 3.7 g ∙ kg-1 of BW and 3.8 g ∙ kg-1 of BW, respectively, of salt cedar. Water consumption differed by breed at 0.8 L/d and 2.1 L/d for Angora and Boer-cross, respectively, but did not differ by period. All goats increased intake of sal tcedar as the amount of the basal ration was reduced. Both breeds lost weight after the final period of the trial when the basal diet was reduced to 0% BW. Crude Protein of salt cedar ranged from 16.0% to 19.6%, and TDN varied from 67.5% to 69.4%. Sodium content of salt cedar varied from 0.1% to 2.4%. Dry Matter content averaged 32.2%. 0092 THE EFFECTS OF REPEATED GOAT BROWSING ON SALT CEDAR Grant Rogers, Cody Scott, Corey Owens Angelo State University, San Angelo, TX, USA Goats increase intake of salt cedar (Tamarix ramosissima Ledeb.) when exposed to the plant in individual pens at weaning. The purpose of this study was to quantify intake of salt cedar on pasture and to determine if goat browsing will reduce the amount of salt cedar cover. Prior to release on salt cedar plots, all goats were individually penned and fed salt cedar for 14 days. Salt cedar was fed each day at 0800 with refusals weighed to estimate intake. The amount of salt cedar was increased daily as intake increased. Once goats were released on plots, bite counts were taken to quantify diet selection. Nine 6 X 12 m plots were established in dense stands of salt cedar. Three plots were stocked with 6 goats for 3 consecutive days. Each plot was exposed to goat browsing for 5 hours each day. Three additional plots were stocked in the same manner and were restocked with 6 goats 30 days later. Three additional plots were not stocked with goats and served as the control. In addition, 20 additional salt cedar plants were individually tagged outside the plots. One half (n=10) trees were completely defoliated with vegetation response recorded. While in individual pens, all goats increased intake daily. In addition, goats readily selected salt cedar in plots. After the three days of foraging, goats reduced salt cedar canopy cover from 30% to 15%. Other results will be discussed. 0093 What should be in a recommended reseeding mixture? Michael Schellenberg, B Biligetu Semiarid Prairie Agricultural Research Centre, Swift Current, Saskatchewan, Canada Increased diversity of plant species in seeded mixtures has been suggested as a means to increase yields and potentially mediate effects of changing climate. The question arises if this is true what would be the appropriate combination for reseeding rangelands? From a forage standpoint, the mixture should include grazable plants such as legumes and grasses. In 2009, a study was initiated at Swift Current, SK Canada using a full factorial random block design with 4 replicates to examine the potential benefit of combining 2 grass species (western wheatgrass, green needle grass) with two legume species (alfalfa and purple prairie clover) in mixture ranging from monocultures to all four species. The dry matter yield results are presented for the year 2010 and 2011. With these two years having above normal precipitation, alfalfa dominated plots (P<0.05) in which it was seeded. Examination of functional group (legume vs. grass) indicates having 2 legumes has the greatest benefit for dry matter production. Seeding legumes resulted in greater production (P<0.05) than grasses alone. The mixtures had trace element and forage quality differences (P<0.0001) that indicated marked differences based on the presence of legumes or grasses. This would suggest the usual recommendation of having a single legume with grasses needs to be reconsidered from a production stand point as well as nutritional quality. 0094 INTAKE OF SALT CEDAR BY TWO BREEDS OF SHEEP Zach Borroum, Cody Scott, Corey Owens Angelo State University, San Angelo, TX, USA Goats will readily consume salt cedar (Tamarix ramosissima Ledeb.) in individual pens and on pasture, but it is unknown if sheep will consume the plant in the same manner. For this project, 10 recently weaned Rambouillet ewe lambs and 10 recently weaned Suffolk ewe lambs were individually penned and fed salt cedar daily along with a basal ration to meet maintenance requirements. In addition, 10 recentlyweaned Boer nannies were placed in individual pens and exposed to the same feeding regime. For the first 14 days, salt cedar was fed each day at 0800 for 30 minutes. Refusals were weighed to estimate intake. The amount of salt cedar fed was increased daily as intake increased. At 0900, the basal ration (2.5% BW) was fed with refusals weighed the next morning to estimate intake. On days 15-28, salt cedar was fed three times a day to estimate the upper limit of salt cedar intake. Animals were weighed every 7 days of the study, and water and a mineral supplement were available ad libitum. Both sheep and goats readily consumed salt cedar and increased intake across the 28 days of feeding. All animals gained weight throughout the study as well. It appears that both sheep and goats will consume salt cedar and may reduce salt cedar cover once released on pasture. Other details of the study will be discussed. 0097 Forage quality variation among accessions of warm-season grasses of North America during summer of semiarid western Canada Bili Biligetu, Mike Schellenberg Semiarid Prairie Agriculture Research Centre, Swift Current, Canada Warm-season grasses with good forage quality can provide forage for cattle after cool-season grasses have reached maturity in the semiarid region of western Canada. The objective of this study was to evaluate forage quality of ecotypes of little bluestem (Schizachyrium scoparium (Michx.) Nash), side-oats grama (Bouteloua curtipendula (Michx.) Torr.), and prairie sandreed (Calamovilfa longifolia (Hook.) Scribn.) during the seed ripening stage of plains rough fescue (Festuca hallii (Vasey) Piper), a coolseason grass. From 2006-2007, randomized complete block field plots were established for each of the grasses near Swift Current, SK, Canada using accessions collected from the Canadian prairies. Forage samples were taken for quality analysis in early July of 2010 and 2011. Eleven accessions of plains rough fescue had similar crude protein (CP) (83 g kg-1), neutral detergent fiber (NDF) (592 g kg-1) concentration, but had different acid detergent fiber concentration (ADF) (ranging 327-366 g kg-1). Accessions of sideoats grama differed for ADF (ranging 263-284 g kg-1), but had similar CP (132 g kg-1) and NDF (638 g kg1). Accessions of prairie sandreed differed for CP (ranging 115-136 g kg-1), but had similar NDF (716 g kg1) and ADF (380 g kg-1). Little bluestem accessions differed for CP (78-101g kg-1) and NDF (669-696 g kg-1) concentrations, but had similar ADF (686 g kg-1). Compared to mature plains rough fescue, side-oats grama had higher CP with a similar fiber concentration, but the other two warm-season grasses contained relatively higher fiber concentrations. 0105 Individual Tree and Plot Level Mesquite (Prosopis glandulosa) Biomass Estimation Using Aerial Imagery Mustafa Mirik, R James Ansley, Brady Surber Texas A&M AgriLife Research, Vernon, TX, USA Both temporal and spatial magnitude, structure, and distribution of rangeland aboveground biomass (AGB) are important inputs for many necessities, in particular for estimating terrestrial carbon amount, ecosystem productivity, climate change studies, and potential bioenergy uses. Much of the remote sensing research previously completed has focused on determining AGB in forested ecosystems with little attention directed to estimate AGB in rangelands. Our objectives were to 1) identify and delineate individual and plot level honey mesquite (Prosopis glandulosa) plants from surrounding live vegetation using two-dimensional (2D) geospatial imagery with a 1-m spatial resolution at two sites and 2) develop regression models relating imagery-derived canopy area and field-measured single tree and plot level dry AGB estimation. The regression results show that there were very close and significant relationships between mesquite plant AGB and canopy area. The individual tree AGB to canopy area relationship was linear with coefficient of determination (r2) > 0.94, whereas plot level AGB and canopy area exhibited curvilinear relationship with r2 > 0.90. These results suggest that spectral reflectance recorded on 2D high resolution imagery is suitable for assessing and quantifying mesquite AGB in rangeland settings. 0106 Feeding the pasture vs. feeding the steer: Interaction of fertilizer and supplement inputs to a wheat grazing system. Ryan Reuter, James Rogers, Jon Biermacher The Samuel Roberts Noble Foundation, Ardmore, OK, USA Producers may consider using several types of inputs in grazing systems, including both agronomic and animal management inputs. This study sought to determine how these two types of inputs may interact in a typical stocker cattle grazing program. Treatments were arranged in a 2x2 factorial with 2 levels of fertilizer and 2 levels of soybean hull supplementation. Twelve 0.81-ha paddocks were established to wheat (Triticum aestivum, var. ‘Endurance’) in the fall of 2011. In a completely random design, paddocks received combinations of 56 or 168 kg per ha of actual nitrogen fertilizer, and soybean hull supplementation at 0 or 0.75% of steer body weight (BW) per day (fed in three feedings per week). Paddocks were stocked with 2 tester steers (initial BW 183 ± 9.5 lb) for 175 d. Tester steers were shrunk in drylot for 16 h and then weighed on calibrated scales. Forage mass in each paddock was measured twice per month with a calibrated electronic plate meter, and stocking rate adjusted with grazer steers to maintain a forage allowance of 2.5 kg of forage DM per kg of steer BW. Dependent variables were subjected to analysis of variance with paddock as the experimental unit. Fertilizer and supplementation inputs both increased the number of grazing days, but neither affected ADG . Consequently, both inputs improved total gain per acre. There was no interaction between the inputs, indicating that a producer should evaluate the economic value of each input independently. 0107 Assessing disturbance regimes of northern temperate pastures using landowner surveys Lysandra Pyle, Edward Bork, Linda Hall University of Alberta, Alberta, Canada The sustainable management of pastures depends heavily on understanding the previous impact of disturbances such as grazing, fire, fertilization, seeding, etc. For example, management actions such as long-term grazing and broadleaf herbicide application for noxious weed control can eliminate standing legume biomass, and even limit their long-term presence in the sward. Despite the importance of this information, little is known of the relative presence, frequency and intensity of historical disturbance regimes in the Aspen Parkland Natural Subregion of central Alberta. Here we report on the results of a survey of pasture landowners completed during the summer of 2012 at 44 locations in and around the Edmonton region. Management history was determined by interviewing landowners to gather information on pasture age, planting history, fertilization regime, and disturbance history, including the timing, frequency, intensity and type of grazing animals, etc. Summary results of this survey containing landowner management behaviour will be linked in future work to soil and vegetation properties sampled in the field in order to link pasture conditions (range health, seed bank composition) with disturbance history. This information should provide range managers with improved information on the relative impact of disturbance regimes on rangeland sustainability. 0108 Patch burn-learning: Rangeland and liberal arts education Devan McGranahan The University of the South, Sewanee, TN, USA Rangeland management has traditionally been under the purview of land-grant universities and research stations focused on productivity and technological development, often from a positivistic scientific perspective. As rangeland management has developed a broader ecological - but no less scientific perspective, the questions asked and methods applied in rangeland science are of interest to schools less associated with rangelands: small liberal arts colleges. Increasingly, liberal arts institutions seek to integrate research and hands-on experience into undergraduate programs. The University of the South, a small liberal arts college in Sewanee, Tennessee, applied patch burn-grazing to an old field pasture as part of an integrated ecology and sustainable agriculture initiative. Goals included restoration and maintenance of a locally under-represented habitat type (open grassland); hands-on student experience with prescribed fire and cattle management; and ultra-local beef production. Students participated in prescribed fire, cattle handling, and ecological monitoring of primary and secondary fire and grazing effects. The on-campus pasture applied landscape ecology to the working landscape in which students live and study, and the Sewanee community will enjoy campus-raised beef in the dining hall. The application of rangeland management principles in the design and study of the grazing system at Sewanee integrates ecology and agriculture in the Environmental Studies curriculum. 0113 To Plow or Not to Plow: Investigating Grassland to Cropland Conversion in the Northern Great Plains Using Systems Dynamics. Benjamin Turner1 ,2, Roger Gates1, Tim Nichols2, Melissa Wuellner2, Luis Tedeschi3 1South Dakota State University, West River Ag Center, Rapid City, SD, USA, 2South Dakota State University, Brookings, SD, USA, 3Texas A&M University, College Station, TX, USA From 1997 to 2007, 23.7 million acres of grassland were converted to cropland. Fifty seven percent were located in the Northern Great Plains (NGP). Since 2007, another 23.7 million U.S. acres have been converted with the majority located in the NGP. These changes were likely driven by prices, U.S. farm programs (e.g. crop insurance) and policies (e.g. ethanol mandates), technology, and social-cultural changes of land owners and managers. The short term positive benefits have been increased food production and returns to farmers. However, there could be unintended consequences through loss of ecosystem services like water quality degradation, wildlife habitat loss/fragmentation, and decreased carbon sequestration. The principal objective of this work was to: 1) identify structural features influencing land use decisions, 2) quantify implications for land management, and 3) forecast potential unintended consequences from those decisions. This will be achieved through triangulation of qualitative and quantitative data using a systems dynamics approach. Triangulation is defined as a procedure to find convergence among different sources of information to form themes in a study. The three spokes of triangulation are: 1) qualitative information gathering through interviews with system stakeholders, 2) using system dynamics modeling to link identifiable themes, and 3) quantitative data incorporation through methodology to test themes and identify potential outcomes. Outcomes include: 1) evaluation of land uses from financial, ecological and managerial perspectives, 2) identifying alternative intervention strategies, and 3) creation of a decision support tool to provide long term insight on NGP land use decisions. 0115 Above and Below Ground Nutrient Cycling in North Dakota Wetlands Lindsey Meyers, Edward DeKeyser, Jack Norland, Christina Hargiss, Thomas DeSutter North Dakota State University, Fargo, ND, USA Nutrient cycling in wetlands provides crucial ecosystem services. Nutrient storage in vegetation and soils can affect wetland quality and function. Over the summer of 2011, 55 wetlands were visited across the state of North Dakota for the National Wetland Condition Assessment (NWCA). An additional 18 wetlands that were treated for hybrid cattail (Typha x glauca) invasion by sediment removal were sampled in the summer of 2012. Plant and soil samples were collected at each wetland site. Samples were taken at three different landscape positions in and adjacent to the wetland: upland, toe slope, and shallow marsh. Plant samples were analyzed for Phosphorus, Carbon, and Nitrogen content and soil samples were analyzed for Phosphorus content. Soil samples collected in conjunction with the NWCA were analyzed for an additional 18 nutrients and soil properties. Several regional wetland assessments were also completed at each site for comparison modeling: the Index of Plant Community Integrity (IPCI), the North Dakota Rapid Assessment (NDRAM), and the Hydrogeomorphic (HGM) Model. Comparisons of resource pools were made between landscape position, soil content, vegetation type, surrounding land use, and wetland condition. Additional results will be presented. 0116 Survival Risk for Pronghorn Exposed to Wind Energy Development on Winter Range in SouthCentral, Wyoming Kaitlyn Taylor, Jeffrey Beck University of Wyoming, Laramie, WY, USA Landscapes with high potential for wind energy development often coincide with suitable habitat for pronghorn (Antilocapra americana). Evaluating the influence of energy development on pronghorn is particularly critical in winter when they encounter elevated energetic demands. The purpose of our study was to evaluate pronghorn survival risk on a landscape developed for wind energy production (Dunlap Ranch [DR]) in comparison to an offsite reference area (Walcott Junction [WJ]) over winters 2010, 20102011, and 2011-2012 in south-central Wyoming, USA. In January 2010 we attached GPS-transmitters to 35 doe pronghorn in each study area. We recovered transmitters from dead animals located via fixedwing flights and from transmitters that detached from living animals at the end of our study. We used 57,660 pronghorn locations to model winter survival risk for individuals from the DR and WJ populations using Cox's proportional hazards model. In our models we incorporated anthropogenic and environmental landscape coverages thought to be important in predicting pronghorn survival. For example, we developed a fence layer using satellite imagery to evaluate the role of fences on pronghorn winter survival. We used the Kaplan-Meier product-limit estimator to estimate survival for each population. Twenty-three pronghorn died in the DR and 10 in the WJ with the majority of deaths occurring in winter 2010-2011 for DR pronghorn and in summer for WJ pronghorn. Survival (Ŝ) was 0.30 (95% CI: 0.14-0.46) in the DR and 0.68 (95% CI: 0.52-0.85) in the WJ at the end of our 2.5-yr study. 0118 Variables Influencing Off-Road Travel in Sonoran Desert Landscapes Douglas Whitbeck University of Arizona, Tucson, AZ, USA Off-road travel can cause major concerns for land managers in arid environments such as those in southwest Arizona. Proper travel management practices require an understanding of the terrain and of those traveling across it. Geographical Information Systems (GIS) software and remote sensing can offer unique methods for approaching these management issues. In this study, GIS software was used to measure relationships between the severity of existing roads and environmental variables in the San Cristobal Valley of the Barry M. Goldwater Air Force Range in southwest Arizona. First, the accuracy of high resolution 24 cm aerial was calculated by ground proofing in each soil type; the overall weighted accuracy was 64.21%. The imagery was then used to identify off road vehicle (full size) paths, their severity (low, moderate, severe, or authorized), and their intention (convenience or non-convenience). GIS software was used to digitize these paths across the entire valley. Spatial statistics were then calculated using geographically weighted regression. Route density by class and intention, slope, and percent vegetation cover rasters were used in the regression model. Results show that the measured variables have a significant influence on off road travel through the valley. Slope had the highest correlation to density of routes of all classes and vegetation cover was the least influential on all route densities. Using GIS and remote sensing, in this sense, is labor and time intensive but can produce results for areas in need of improved travel and land management. 0122 Potential management of Kentucky bluegrass (Poa pratensis) to increase native prairie diversity in the Northern Great Plains Jonathan Quast1, Shawn DeKeyser1, Amy Ganguli1, Steven Travers1, Cami Dixon2 1North Dakota State University, Fargo, ND, USA, 2US Fish and Wildlife Service, Woodworth, ND, USA Kentucky bluegrass (Poa pratensis) has invaded thousands of rangeland hectares in the Northern Great Plains. Kentucky bluegrass and other invasive perennial grasses along with historic management have changed the prairie from a heterogeneous to a homogeneous landscape resulting in losses of diversity and richness. The influence of Kentucky bluegrass management efforts towards maintaining diversity are addressed in this research by studying the techniques used by federal, state, private, and non-profit land managers. Techniques used by the USFWS Native Prairie Adaptive Management Program as well as more novel techniques being utilized by private, state, and non-profit managers included burning, grazing, a combination of burning and grazing, and rest. Within these parameters, variation to the techniques also occurred such as Spring, Summer or Fall burning, timing and intensity of grazing (winter/early spring, High Intensity/Low Frequency, and adaptive grazing), and species of animal grazing (cattle and bison). Plant community sampling was conducted using modified Whittaker plots to detect species richness and diversity as well as using ocular estimation to determine percent cover within the plot on each native prairie site. The number of plots at each site was determined based on the number of acres with a minimum of three plots per site. Using a 10-pin frame, we estimated the percent of bare ground within each plot as well. Statistical analysis reveals species diversity responses to management techniques, and landscape level patterns in species and community characteristics. 0126 Complexity of Rangelands within Canadian Forces Base Wainwright Tennille Kupsch Alberta Envirnoment and Sustainable Resource Development, Red Deer, Alberta, Canada The Central Parkland Natural Subregion is a complex area in Alberta due to the mosaic of vegetative communities ranging from prairie grassland to forest. To further add to the complexity, CFB Wainwright, a military training base, is located in this natural subregion, consisting of 148 590 acres on which 28,400 AUM's of grazing can be allotted to four different grazing associations. The focus of the project was to provide accurate and up- to- date vegetation and range resource inventories as well as recommendations to address any issues or limitations in current range management practices to ensure the ecological integrity and diversity is maintained on CFB Wainwright. Range Vegetation Inventory and Range Health Assessments were completed to determine what communities are present on that landscape as well as determining the health of those rangelands. The area is unique as it consists of majority of sandy soils with high fire interval. Data was collected for six years with over 600 range inventories being completed. After conducting statistical analysis, 32 plant communities were determined in that area, ranging from early seral burnt communities to climax deciduous communities. Additionally 1 310 Range Health points were completed; of those 451 were healthy, 540 healthy with problems and 319 unhealthy. By knowing range health information in combination with digital resource mapping, appropriate management strategies can be developed on areas that are of concern or deteriorating to ensure the ecological integrity and diversity is maintained on CFB Wainwright. 0127 Monitoring Grazinglands with Satellite Imagery Sarah Goslee USDA-ARS, University Park, PA, USA While Landsat data has been used to monitor primary production in range and pasture areas, such monitoring has generally been intended to track broad changes across multiple years. With an 8-day return time and 30m resolution, Landsat data can be used to assess intra-annual changes, even within rotationally-grazed paddocks. Such detailed monitoring requires careful attention to the correction of atmospheric effects and illumination angle; biases introduced by these factors can overwhelm the changes of interest. Two cases are of particular interest: standardizing images of a single site within a single growing season so that production can be tracked, and standardizing multiple scenes from a single date to enable comparison of multiple sites. A standardized procedure for tracking primary production in grazed systems has been developed and tested with retrospective data. This protocol has been largely automated for use across large areas and multiple times, and can be used in conjunction with 250m MODIS NDVI images to efficiently monitor regional and site changes in production, thereby improving both farm management and regional planning. 0128 Micoclimate modification effects on the species composition of BC grassland communities Donald Thompson0, Barbara Wheatley0, Michael Schellenberg0, Mathilde Leborgne0 1Lethbridge Research Centre, Lethbridge/AB, Canada, 2Grassland Applied Tech Centre, Kamloops/BC, Canada, 3SemiArid Prairie Agicultural Research Centre, Swift Current/SK, Canada, 4VetAgro Sup, Lempdes, France Climate change models for interior BC predict warming and a shift in precipitation from summer to winter. We simulated warming with open top chambers (OTC) and the precipitation shift by covering the plots with a transparent roof in May or June, followed by applying the water excluded back in October. We also included 3 clipping treatments to simulate season of grazing (June clip, Sept. clip. or no clip). Treatment combinations (2x3x3 factorial design) were randomly located within each of 4 blocks at three sites varying in elevation. At the lower grassland (LG) site the OTC's and rainout increased the cover of Sandberg bluegrass while clipping reduced the cover of bluebunch wheatgrass. In the middle grassland (MG) cover of bluebunch wheatgrass increased in the OTC's but and again decreased with clipping. Rosy pussytoes increased with the May rainout. In the upper grasslands (UG) the OTC's reduced the cover of timber milkvetch while clipping increased its cover. Species diversity(H') was unaffected by any treatment at LG, was reduced by rainout at MG and increased by clipping at UG. Even after one year of these treatments some changes in species abundances were noticable. Cumulative effects of three years of treatments are under investigation. 0129 Restoring perennial grasses following downy brome (Bromus tectorum) control with burning and imazapic in salt desert shrublands Beth Fowers1, Thomas Monaco3, Christopher Call0 1University of Wyoming, Laramie, WY, USA, 2Utah State University, Logan, UT, USA, 3USDA-ARS, Logan, UT, USA Downy brome degrades wildlife habitat and produces inconsistent livestock forage in salt desert shrublands of the Great Basin. Most research has focused on reducing downy brome abundance at small-plot scales rather than changing vegetation trajectories at larger scales. A large-scale demonstration study was implemented in 2008 at two salt desert shrubland sites to evaluate how treatments impact downy brome seed bank density, canopy cover and plant density, and seeded perennial grass density. We applied single and combined treatments of late-fall burning to reduce litter and destroy seeds, and late-fall imazapic at 71.6 g ai ha-1 to reduce seedling growth and survival to large (8-15 ha) plots. Sites were then drill seeded with a mix of native and introduced perennial grasses at 7-14 kg ha-1 PLS. Downy brome seed bank densities were 10-fold greater in litter than soil fractions. Significant treatments typically reduced seed bank densities by 50-70%. Downy brome density increased from 2001,500 plants m-2 in untreated plots from 2009 to 2011. Burning and herbicide independently reduced downy brome density and cover; however, when combined, reductions were nearly always significantly more pronounced. Although seeded perennial grass density significantly decreased from 2010 to 2011 across all treatment combinations, both burning and herbicide treatments significantly increased seedling establishment, and when combined, between 4-6 plants m-2 were established in the burning/herbicide treatment. Results indicate that impacting both reproduction and productivity of downy brome, combined with successful perennial grass establishment alter the underlying demographic and competitive processes that initiate desirable ecosystem trajectories. 0131 Sustainable Rangelands: Assessing contributions to food security Kristie Maczko1 ,2, John Tanaka2 ,1, John Ritten4, Jack Morgan7, Netra Chhetri3, Lori Hidinger3, Matt Reeves6, John Mitchell5 1Sustainable Rangelands Roundtable - University of Wyoming, Laramie, WY, USA, 2University of Wyoming - Department of Ecosystem Science and Management, Laramie, WY, USA, 3Consortium for Science, Policy and Outcomes - Arizona State University, Tempe, AZ, USA, 4University of Wyoming Department of Agricultural and Applied Economics, Laramie, WY, USA, 5USDA Forest Service Rocky Mountain Research Station, Fort Collins, CO, USA, 6USDA Forest Service Rocky Mountain Research Station, Missoula, MT, USA, 7USDA ARS - Rangeland Resources Research Unit, Fort Collins, CO, USA Food production from rangelands in the United States will face challenges, as the 21st century progresses, from changing land use priorities, population growth, loss of open agricultural spaces, and climate change. The latter may include increasing temperature, variable rainfall patterns, frequency of extreme weather, severity of invasive species and pest infestations, and changes in livestock forage quantity and quality. These impacts will not only affect food production, but other ecosystem goods and services from agro-ecosystems including rangelands. Changes in U.S. food production systems may ripple around the world because the U.S. is the world's primary grain producer and markets for many food products are global. In adapting to climate change, farmers may relocate to follow favorable climatic conditions, potentially displacing ecosystems and impacting biodiversity and other services ecosystems provide. Concerns surrounding future food production in the U.S. also involve sustainable energy use, genetic biodiversity, and environmental impacts. Land managers (e.g. farmers and ranchers) are among the most important stakeholders in managing agro-biodiversity, food security and rangeland sustainability. Key questions focus on quantifying how much food comes from rangelands and how much food production will change over time due to environmental stresses or policy modifications. Sustainable Rangelands Roundtable participants used an indicator-based system to assess rangelands' contributions to food security and ecosystem services and modeled meat production from cattle, sheep and meat goats. Relationships between meat production and resilience of rangelands to predicted changes in climatic change, invasive species, fire regimes, land use policies, population, and income, remains to be assessed. 0136 Using grazing management and fire to restore Texas Gulf Coastal Prairie Josh Britt, M.M. Kothmann Texas A&M University, College Station, TX, USA A combination of high intensity rotational grazing and a plan for recurring prescribed fire were implemented in Spring 2012 on a 940 acre degraded rangeland in the Gulf Coastal Prairie region of Texas. The area receives about 45 inches of annual precipitation. The soils are primarily loamy sands and sandy loams, with scattered clays causing water to pond in the lower areas. The rotational grazing system utilizes a five-paddock herd rotation. Three paddocks of roughly equal area represent approximately half of the acreage, with one large paddock representing the other half. The smaller paddocks are grazed an average of 7 days and the large paddock up to one month. Length of stay varies depending on forage availability and rate of growth. Monitoring of the pasture through cover data and bimonthly photopoints and utilization estimates began in Summer 2011. The goal of this project is to shift the species composition from various introduced Paspalum species back to native grass species and reduce the abundance of the invasive shrub Macartney rose (Rosa bracteata), while maintaining or increasing the number of animals in the cattle herd. It is hypothesized that over time these practices will promote plant succession and restore the area to a more diverse ecosystem that will support sustainable quail populations, increase the carrying capacity for livestock, and improve overall system health and stability. Initial data showed mean rose canopy cover to be 20%. Total mean canopy cover for all shrub species was 23%. Other initial results will be presented. 0141 Predicting litter and live herb fuel consumption during prescribed fires in native and old-field upland pine savannas of the southeastern United States Angela Reid1, Kevin Robertson1, Tracy Hmielowski2 1Tall Timbers Research Station and Land Conservancy, Tallahassee, FL, USA, 2Louisiana State University, Baton Rouge, LA, USA This project identified predictors of fuel consumption for the dominant fuel bed components (litter (<0.6cm diameter dead material) and live herbs) during 217 prescribed fires in native longleaf pine (Pinus palustris Mill.) and old-field loblolly pine (Pinus taeda L.) - shortleaf pine (Pinus echinata Mill.) savannas in the southeastern United States. Additionally, these data were used to validate the First Order Fire Effects Model (FOFEM) fuel consumption computer model using custom and default fuel loads. Regression models using empirical data suggested that litter and live herb fuel consumption can be predicted by the combined effects of prefire litter and live herb fuel loads, litter and live herb fuel moisture, litter fuel bed bulk density, season of burn, years since fire, days since last rain ≥0.64 cm, relative humidity, energy release component, community type, pine and hardwood basal areas, and the Keetch-Byram drought index. FOFEM's prediction of fuel consumption for litter, live herbs, and duff combined using default fuel loads was 1.5 times the measured fuel consumption (where duff fuel load was zero). Refinement of FOFEM's fuel load and consumption calculations in the studied community types using the newly collected data and suggested model improvements would provide more accurate air quality inventories and assist in guiding appropriate regulation of prescribed fire. Validation of widely used fuel models is important to undertake for all ecosystems using prescribed fire as a management tool to ensure the best possible predictions. 0142 In situ disappearance of bypass DNA from sagebrush plants in cattle by qPCR Jose Alberto Perez-Amaro1, David Bohnert2, Tim DelCurto3, Chad Boyd4, Jeff Leonard1, Yudith NietoCarrillo1, Elieter Gricel Urena-Armas5, Ricardo Mata-Gonzalez1 1Oregon State University, Corvallis, OR, USA, 2Eastern Oregon Agricultural Research Center, Burns, OR, USA, 3Eastern Oregon Agricultural Research Center, Union, OR, USA, 4U.S.D.A.-ARS, Eastern Oregon Agricultural Research Center, Burns, OR, USA, 5University of Papaloapan, Loma Bonita, OC, Mexico Studying how plant DNA is affected by livestock digestion is an intrinsic, poorly understood aspect of molecular dietary analysis in sagebrush ecosystems. The aim of this study was to measure In situ DNA disappearance at various time intervals by the Cy0-qPCR method. In this experiment, values of sagebrush DNA digestibility of freeze-dried and oven-dried ground materials were determined for triplicates using nylon bags containing 4 g of sagebrush leaves. Plant leaf tissues were digested in the reticule-rumen at 00, 4, 8, 16, 24, 36 48, 72, 96, and 120 hours and DNA was extracted by QIAamp DNA mini Kit. To describe progression of DNA degradation, fluorogenic probes and primers were used to detect and quantify the residual amplifiable NTS 5S rRNA region of sagebrush species in Real-Time qPCR analysis. Due to the sensitivity of analysis on starting DNA template, 10 ng of extracted DNA was measured using the Qubit 2.0 fluorometer. DNA disappearance through incubation times differed among treatments, and in general, were higher in the freeze-dried method (P<0.05). The extents of percentage of DNA degradation in the freeze-dried samples were 2.79%, 58.28% and 73.97% as opposed to 1.74%, 32.30% and 43.73% in the oven-dried samples at 4, 48, and 120 h, respectively. In conclusion, the Cy0qPCR method makes it possible to determine the presence of un-degraded and amplifiable DNA regions from sagebrush genome. By quantifying bypass DNA, it may be possible to develop and apply correction factors for DNA-based cattle diet determinations. 0144 Two year regrowth patterns of three key forage species in the southern interior of British Columbia Vanessa Volpatti, Wendy Gardner Thompson Rivers University, Kamloops, British Columbia, Canada In the southern interior of British Columbia, Canada the dominant grass species that exist change along an elevation gradient with bluebunch wheatgrass (Pseudoroegneri spicata) dominating in the lower grasslands, rough fescue (Festuca campestris) in the upper grasslands, and pinegrass (Calamagrotis rubescens) in the forested areas. Understanding how these species respond to grazing, specifically intensity and timing, is important for management. For this study, a site excluding grazing was located for each species: lower grasslands (550 m) for bluebunch wheatgrass, upper grasslands (900m) for rough fescue, and in the interior douglas-fir zone (1000m) for pinegrass and data was collected over two growing seasons, 2011 and 2012. At each site 30 plants where selected and randomly assigned a treatment of a one-time clipping at a 40 or 70 percent removal of photoshynthetic material. After clipping, 10 tillers per plant were marked and these tillers were measured for regrowth 5, 7, 9, 11, and 13 days after clipping. Probes at the base of each plant were used to collect soil moisture and temperature. In year one, each species responded differently. Bluebunch wheatgrass clipped at a higher intensity regrew significantly slower than the low intensity treatment while rough fescue and pinegrass showed no significant treatment effect. In year 2, there were no treatments effects on any of the species. Soil moisture was shown to have a relationship with regrowth. Varied regrowth between species and years helps to show that adaptive management for individual species may work better than a single management strategy. 0146 Land degradation in a Mexican tropical dryland: An evaluation using state-and-transition models Hector Godínez-Alvarez, Itzel Cabral, Mayra Hernández, Rafael Lira, Daniel Muñoz, Leticia RíosCasanova UBIPRO, FES Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, Mexico Land degradation is an environmental problem in drylands resulting from factors such as climate variation and human activities. The state-and-transition models are synthetic descriptions of the dynamics of vegetation and soil surface at particular sites. They are used to determine the processes driving ecosystems and the way in which management affect such processes. They are also used to determine the factors associated with land degradation. The Zapotitlan Valley is a tropical dryland in central Mexico with land degradation problems because land-use change and habitat fragmentation. The goal of this work was to evaluate land degradation using state-and-transition models. The work was conducted in fluvial terraces that were classified according to soil texture and vegetation cover. At each terrace, we conducted 3-5 transects to obtain data on soil properties, vegetation traits, and human activities. With these data quantitative indicators were calculated and models were built. Indicators suggested that terraces differ in vegetation and soil traits. There were no clear differences in human activities. The model has four states (closed canopy mesquite shrublands, open canopy mesquite shrublands, field crops, and badlands) and five transitions among them. There were two reversible transitions. The processes associated to land degradation are primary productivity, water and nutrient cycles. The management actions affecting these processes are firewood collection and sowing. This is the first model proposed to understand land degradation in this region; therefore it is necessary to continue improving it through historical information, local and ecological knowledge, and experimental data. 0149 The interactive effects of prescribed fire and ungulate grazing on bankrupt bush (Seriphium plumosum) in a South African highveld grassland. Michele D. Clark1, William E. Rogers1, Carissa L. Wonkka1, Deseri Nally1, W. Richard Teague2, Urs P. Kreuter1, James P. Muir3 1Texas A&M University, College Station, Texas, USA, 2Texas A&M AgriLife Research and Extension Center, Vernon, Texas, USA, 3Ukulima Farm Research Center-Borlaug Institute, Alma, Limpopo Province, South Africa Encroachment by Seriphium plumosum (bankrupt bush) is degrading South African rangeland ecosystems by converting productive grasslands into largely unpalatable shrublands. The factors responsible for its increased abundance are poorly understood and experimental assessments of potential management strategies are lacking. We established a full-factorial, completely randomized experiment to examine the interactive effects of prescribed fire and ungulate grazing on Seriphium and herbaceous community structure in a South African highveld grassland. The experiment was located on a leased pasture with 400 cow/calf pair of Bonsmara cattle. We established sixteen 10x10m plots. Randomly assigned treatments included i) burned and fenced, ii) burned, not fenced, iii) fenced, not burned, and iv) unburned, unfenced. Pre-treatment Seriphium demography, plant community, and soils data were collected and grazing exclosures were erected in July 2012. Cattle exclosures consisted of 4strands of evenly spaced barbed wire to a height of 2m. Independent prescribed fires were conducted for each burned plot in July 2012. Flame heights frequently exceeded 4m and aboveground foliage was fully consumed including all the leaves of Seriphium shrubs. This study will provide important insights into the factors influencing Seriphium growth and survival and will assist with the development of management efforts that attempt to mitigate its continued degradation of South African rangelands. 0154 Evaluating Festuca campestris plug establishment along slope environments Laura McGregor1, Steve Tannas2 1University of Guelph, Guelph, Ontario, Canada, 2Foothills Fescue Reseach Institute, Calgary, Alberta, Canada Current restoration techniques in Alberta's threatened fescue grasslands are not often successful due to low seedling survival, high cost and limited availability of seed. The goal of this research was to evaluate the establishment and vigour of Festuca campestris (foothills rough fescue) plugs in various microclimates along slopes in Southern Alberta. A field experiment was initiated in June 2011 on two separate slopes with three slope locations (lower, middle, upper) outside of Cochrane, Alberta and a separate upland control on black chernozemic soil near Cremona, Alberta for comparison to the lower slope positions. A total of 36 plots were planted with a split-plot design, half Festuca campestris planted alone and half planted with Potentilla fruticosa (shrubby cinquefoil). Variables monitored on each slope position consisted of soil type, available NPK, CEC, pH, organic matter and monthly soil moisture Vigour measurements of tiller number, height and basal diameter and climatic data were collected from June 2011 to August 2012. Initial results show that the plug survival rate was high at all sites. There was an increase in mean plant volume and tillering from June to August of year one and then again from year one to year two. The plots on black chernozemic soil had significantly more tillering then the lower slope position. Overall Festuca campestris plugs were able to establish in challenging microclimates along a slope and other factors such as competition may influence plug growth. The use of plugs shows promise in the restoration of rough fescue plant communities. 0155 Reduced Establishment of Canada Thistle in Planted Grasslands Using a High Density of Planted Forbs Jack Norland1, Cami Dixon2, Kristine Askerooth3, Kyle Kelsey4, Guojie Wang5 1North Dakota State University, Fargo, ND, USA, 2USFWS/Dakota Zone Biologist, Woodworth, ND, USA, 3Tewaukon NWR, Cayuga, ND, USA, 4Madison Wetland Management District, Madison, SD, USA, 5Central Grasslands Research Extension Center, Streeter, ND, USA The control of Canada thistle (Cirsium arvense) in new grassland plantings is costly often reducing desirable forbs and the vegetative diversity of the planted grassland. Spiking native seed mixtures used for planting grasslands with a high density, 4-10 times the typically recommended seeding density (300 seeds/m2), of 3-5 native forbs that are functionally similar to Canada thistle is theorized to reduce Canada thistle establishment in planted grasslands. The use of functionally similar species at high seed densities is to provide an aversive environment for Canada thistle limiting their establishment. An experiment to test the spiked method consisted of small-scale (4 x4 m) and large-scale (>400 m2) plot experiments with native and spiked plantings in a paired plot design. The small plot spiked treatment had significantly lower (p<0.05) percent Canada thistle cover (spiked 4.6, native planting 14.9) and density (spiked 4.9, native planting 11.6 plants/m 2). The large plots had the same response with the spiked treatment having significantly lower (p<0.05) percent cover of Canada thistle (spiked 2.8, native planting 6.3) and density (spiked 5.3, native planting 13 plants/m 2). A survey of the large plots found the spiked treatment had a significantly lower amount of area with less than 10% cover of Canada thistle. The results indicate that the spiked treatments reduced the establishment of Canada thistle immediately after seeding. An economic analysis found that the cost of the spike treatment would be similar to the cost of spraying Canada thistle in the native planting over a 10 year period. 0156 Soil seed bank dynamics of white locoweed (Oxytropis sericea) infested plots in relation to targeted grazing with sheep and herbicide application Stephanie Lopez, Andres Cibils, Laura Goodman, Laurie Abbott, Keisha Yazzie, Kirk McDaniel New Mexico State University, Las Cruces, NM, USA White locoweed exhibits extreme population cycles driven by germination pulses that are supported by vigorous soil seed banks (SSB) which can contain several thousand seeds per m 2. Given that sheep avidly consume locoweed flowering heads and seed pods, we predicted that targeted grazing (TG) would be as effective as herbicides in reducing the number of viable seeds in the SSB at three sites in northern New Mexico. Thirty-six soil cores were collected from nine 200m 2 plots that had received: a) TG with sheep in two consecutive growing seasons; or b) a single application of Picloram P+D; or c) control. Sheep fecal pellets were also collected from all grazed plots. White locoweed seeds were manually separated from soil cores and fecal samples, classified into current (light) or previous season (dark) seed crops, counted, and then subjected to surface sterilization and mechanical scarification. Seeds were then placed in a germinator for six weeks. Daily temperature cycles were set at 7 oC for 10h and 13oC for 14h to simulate spring day and night soil temperatures at our research sites. Germination rates of seeds recovered from TG (89%) and control (88%) plots were similar. A trend toward reduced germination rates in herbicide plots (77%) was observed (P=0.11). Proportion of current vs. previous season seeds was similar among treatments. None of the 23 seeds recovered from sheep fecal pellets germinated. Number of white locoweed seeds in the SSB and their germination rates were not reduced with TG but were slightly diminished with herbicide application. 0158 Linkages between livestock-raising and acequia irrigation farming in rural communities of northern New Mexico: a preliminary assessment Stephanie Lopez, Andres Cibils, Ursula Smedly, Steven Guldan, Alexander Fernald New Mexico State University, Las Cruces, NM, USA Acequia irrigation farming communities of northern NM were founded over 300 years ago during the Spanish colonial period. We conducted a preliminary study to assess the role played by livestock-raising in three farming communities of the area. We conducted unstructured interviews with community leaders and analyzed long-term livestock inventories, precipitation and Palmer Drought Severity Index records, and hay production data. State, county (Rio Arriba and Taos), and watershed level data were obtained for livestock inventories. Climate and crop production were only assessed at the county level. Community leaders whom we spoke with raised livestock and viewed this activity as a central component of their agricultural enterprise. Livestock-raising was characterized as providing financial stability in the face of climatic uncertainty. Farming in the valley and livestock-raising on upland rangelands were described as inextricably linked endeavors since the days of Spanish settlement. Reduced participation in livestockraising by younger generations was viewed with concern. Long term downward trends in state and county livestock inventories were positively correlated with each other. Time series breakpoints (permanent change in numbers) in livestock inventories differed by livestock species and county, and appeared to be associated with droughts only in the case of cattle. Availability of labor, public land use policy, and termination of government incentive programs were the presumed causes of breakpoints in sheep inventories. Correlation between acres in hay production and cattle inventories at the county level became stronger during the 2000-2010 decade suggesting a possible increase in the strength of valleyupland linkages in recent years. 0159 Impact of Temperature, Defoliation and Competition on a Selected C3 and C4 Grass Mahesh Khadka Thompson Rivers University, Kamloops, Canada Plant communities are impacted by climate change and an increase in global temperature may impact how native grass species respond to inter and intra specific competition and defoliation. A greenhouse study was conducted with the objective of understanding the competition and impact of grazing on a native warm season and cool season grass under different temperature regimes. Temperature, competition and defoliation were altered on one C3- western wheat grass (Agropyron smithii) and one C4- little blue stem (Andropogan scoparius) and total above and below ground production was measured. There were two temperature treatments: 20°C and 30°C, and 5 competition treatments: C3 (1 plant/pot), C3 (4 plants/pot), C4 (1 plant per/pot), C4 (4 plants/pot), and C3/C4 combination (2 plants of each per pot). The defoliation treatments altered frequency (either clipping 1 time or clipping 3 times each a week apart) and intensity (either moderate with removal of 50% of vegetation or heavy with removal of 75% of vegetation). This resulted in a total of 5 different defoliation treatments: control (no clipping), low frequency-moderate intensity, high frequency-moderate intensity, low frequency-high intensity, and high frequency-high intensity. All treatments were replicated 8 times. The results showed that western wheatgrass out competed little blue stem in growth and total biomass production but the difference was less under the higher temperature treatment. For both species biomass production was not affected if the frequency of defoliation was low, regardless of intensity. 0160 Environmental characterization of rangeland pastures in piñon-juniper grassland mosaics in central New Mexico Vanessa J. Prileson, Andrés F. Cibils, Michaela Buenemann, Caitriana M. Steele, Eric J. Schollegerdes, Darren K. James, Richard L. Dunlap, Shad H. Cox, Wendy Taylor, Stephanie López New Mexico State University, Las Cruces, NM, USA Describing the biotic and abiotic characteristics of rangeland grazing environments is critical to explaining spatial distribution of grazing livestock. We characterized the environment of two adjacent, 300 acre pastures containing piñon-juniper grassland mosaics during spring, summer, fall, and winter in two consecutive years. Landsat-derived NDVI, herbaceous vegetation cover, forage biomass, chemical (nutrient) composition, and weather variables were measured in grassland ridges, grassland swales, open woodlands (8 – 19% tree cover) and closed woodlands (32 – 37% tree cover). A mixed-model ANOVA was used to analyze the effects of year, season, and vegetation type on all environmental variables. Most vegetation and weather variables varied across seasons (P<0.05), year (P<0.05), and vegetation types. NDVI and forage NDF values were higher in woodlands than in grasslands (P<0.05) while forage biomass, CP content, and herbaceous vegetation cover were higher in grasslands than woodlands (P<0.05). Forage biomass and chemical composition of open woodlands and grasslands was similar (P>0.05). Closed woodlands had detectably less herbaceous cover and forage biomass (P<0.01). Forage chemical composition did not differ (P>0.05) between open and closed woodlands. Average ambient temperatures were similar in woodlands and grasslands (P>0.05) across all seasons. Maximum ambient temperatures and average wind chill corrected temperatures were warmer in woodlands than in grasslands (P<0.01) across all seasons. Average wind speed decreased with increasing tree cover (P<0.01). Results confirm earlier studies at this site, which suggested open woodlands can offer similar quality and quantity of forage and more opportunities for thermal comfort in cooler weather compared to adjacent grasslands. 0162 COMPARING LIVESTOCK GRAZING EFFECTS ON PLANT FOLIAR COVER IN MONGOLIA ACROSS ECOLOGICAL SITES AND ZONES Chantsallkham Jamsranjav, Robin Reid, Maria Fernandez-Gimenez Colorado State University, Fort Collins, Colorado, USA Mongolia's rangelands span a range of ecological zones expected to respond differently to grazing, with implications for appropriate grazing management and policy. Few studies have compared grazing effects across multiple ecological zones in Mongolia or accounted for within-zone differences in grazing responses by soil type. To address these gaps, we investigated the effects of livestock grazing on plant cover within different ecological sites in three Mongolian ecological zones. We measured cover by by line point intercept in plots located along grazing gradient from herder winter camps at 100 m, 500 m and 1000 m from each camp, assuming grazing pressure is highest close to the winter camp and decreases with distance away from the camp. Ecological sites were classified based on soil and environmental characteristics sampled at each plot. Based on existing theories of rangeland dynamics and past research in Mongolian ecosystems, we hypothesized that a) plots closest to impacts points will be associated with a shift from grasses to forbs, and from palatable to unpalatable plants relative to more distanst plots, and b) these effects will be strongest in the forest-steppe and weakest in the desert-steppe. Field observations adn preliminary analyses suggest these hypothese are supported by our data. By evaluating rangeland dynamics in relation to both soils and ecological zones, this study contributes to development of appropriate management and policy responses for different ecological zones. 0165 Seed production of Jojoba in the Matorral area of central Sonora, Mexico. Martha Martin1, Fernando Ibarra1, Lilia Alcaraz3, Salomon Moreno1, Francisco Denogean1, Felix Ayala1, Fernando Ibarra1, Jassany Fuentes1, Heriberto Leiva1, Ramon Amarillas1 1University of Sonora, Santa Ana, Sonora, Mexico, 2University of Sonora, Hermosillo, Sonora, Mexico, 3CIBNOR, La Paz, Baja California Sur, Mexico Jojoba (Simmondsia chinensis) is a native forage shrub important for livestock and wildlife in the Sonoran Desert. Recently, severe droughts, frost and overgrazing have caused a drastic density decline. Seed production under irrigation has declined and is no longer an option and information on seed production of natural communities for range rehabilitation is lacking. The objective of this study was to calculate seed production potential in the matorral area during the spring summer and fall of 2012. Three ranches were selected around Santa Ana, Sonora, Mexico. We measured plant density, height, canopy cover, number of seeds per plant and total seed production. Site selection within ranches was conducted at random. Descriptive statistics were used for analyses. Precipitation was 35% below average. Plant densities were very heterogeneous between ranches and varied from 9 to 95 plants/ha (35.8±9.5 average). Plant height varied from 1.7 to 3.2 m (2.1±0.23 average). Canopy cover varied from 0.9 to 5.8 m 2 (2.25 m2 average). The number of seeds per plant varied from 69 to 1109 (397 average). Seed production varied from 45 to 721 grams/plant (258±27.2 average). Total seed production of Jojoba on a year under below average precipitation may vary from 0.40 to 68.0 kilograms (9.2 average) of clean seeds/ha. We conclude that the amount of seed harvested in this study is sufficient for ranchers to justify the use of this practice. Nearby areas with higher jojoba densities may represent a better option for harvesting considering the high cost of seed. 0167 Seed harvesting of native plants to improve income and natural resources sustainability on a rural area at central Sonora, México. Martha Martin1, Fernando Ibarra1, Salomon Moreno1, Teodoro Ramos2, Hector Montano2, Alberto Ibarra2, Ramon Clavero3, Felix Ayala1, Fernando Ibarra1, Francisco Denogean1 1University of Sonora, Santa Ana, Sonora, Mexico, 2San Francisco Timmins Mining Co., LLano, Sonora, Mexico, 3STPS Gobierno del Estado, Hermosillo, Sonora, Mexico Llano, Sonora is a small community located 100 miles north from Hermosillo, Sonora. Habitants are currently struggling due to lack of employment. Activities such as agriculture, ranching, mining, wood production, charcoal extraction, and brick construction have left deteriorated rangelands. Aware of this, the University of Sonora, San Francisco Timmins Mining Co. and the employment office of the State Government of Sonora joined efforts and start a training course in natural resources management to teach inhabitants of Llano to take care of the environment and teach them to use native vegetation as a source of income in a sustainable way. Twenty five unemployed mature woman and man were hired to be trained for 60 days. Classroom lectures and field work was designated for preparation. They were trained to harvest and manage seeds of Palo fierro (Olneya tesota) Palo verde (Cercidium microphyllum), and Mezquite (Prosopis juliflora) to be either sold or used for range seeding in their own communal land. Results were successful and encouraging. People were very motivated and interested in participating on solving the problem. Each person was able to harvest from 0.650 to 1.5 kg of clean seed/day. This yields a profit of $130 to $195 pesos/day, which is two to three times greater than the official daily minimum wage for the area. We conclude that this extra income is sufficient for a family to survive and may aid in a more efficient management of natural resources. 0168 Long-term Effects of Roller Chopping and Fire on Invasion of Exotic Grasses Johanna M. Prukop1, J. Alfonso Ortega-S1, Felix Ayala-Alvarez2, Timothy E. Fulbright1 1Caesar Kleberg Wildlife Research Institute, Kingsville, Texas, USA, 2Universidad de Sonora, Santa Ana, Sonora, Mexico The invasion of exotic grasses into South Texas native rangelands is affecting the ecology, leading to decreased biodiversity. Practices such as roller chopping used to reduce brush vegetation and encourage herbaceous cover, may also favor the invasion of exotic grasses. Prescribed burning may be used to prolong the life of roller chopping, however, it may also encourage the establishment of invasive plants. The hypothesis that prescribed fire can prolong the effects of roller chopping and re-seeding is being tested. The study is being conducted at the Welder Wildlife Foundation Refuge near Sinton, TX. The study area consists of nine plots. During the Fall of 2007, three treatments where evaluated: roller chopping, roller chopping plus seeding a native plant mix, and the control. Currently, with the addition of prescribed fire done in 2011, five treatments are currently being evaluated: roller chopping, roller chopping and prescribed fire, roller chopping plus seeding, roller chopping plus seeding and prescribed fire, and the control (no manipulation). Since 2011, data has been collected for percentage of canopy cover of exotic grasses, native grasses, litter and forbs. Woody plant cover data using the line intercept method will be presented. Information on percent canopy cover of exotic vegetation, percent canopy cover of native vegetation, and percent canopy cover of forage available to deer and quail species will be presented. 0170 Nutritive value and cattle preferences in an old Boer lovegrass stand treated with biosolids in Cananea, Sonora, Mexico. Fernando Ibarra1, Martha Martin1, Roque Martinez1, Felix Ayala1, Francisco Denogean1, Salomon Moreno1, Rafael Retes2, Fernando Ibarra1, Ana Martinez1 1University of Sonora, Santa Ana, Sonora, Mexico, 2University of Sonora, Hermosillo, Sonora, Mexico Boer lovegrass (Eragrostis curvula var. conferta) is an introduced species planted to increase productivity on deteriorated rangelands in northern Mexico. This study was conducted during 2008 at Cananea, Sonora, Mexico to evaluate nutritive value and cattle preferences on a 15 years old Boer lovegrass stand three summers following biosolids application at rates of 0, 25, and 50 ton/ha. Biosolids were handapplied during 2006 on triplicate 5x5 m plots. Variables evaluated were crude protein, digestibility and animal preference. A mature Hereford cow was used to test grazing preferences during the summer of 2008. Three grazing periods of 60 minutes each were allowed and each was considered as replication. A different cow was used on each grazing event. Time spent grazing was recorded on each plot. Data was analyzed by ANOVA (P<0.05). Precipitation was average during the study period. Results show that crude protein averaged 5.1% in the controls and varied from 7.5-9.9% on treated plots. Digestibility averaged 37.1% in controls and varied from 40.5-46.3% on treated plots. Cattle preferred (P<0.05) to graze Boer lovegrass plants on plots treated with biosolids. Cows spent 28.2 (15.7%), 58.5 (32.5%), and 93.2 (51.8%) minutes on plots treated with biosolids at rates of 0, 25 and 50 tons/ha, respectively. Overall, cows spent 84.3% of the time grazing plants on biosolid treated plots. We conclude that nutritive effect of biosolids continues even three growing seasons after treatment application. The uses of these nutrient-rich organic materials play an important role on the improvement of deteriorated rangelands. 0171 Vegetation changes following biosolid applications in a short grassland at northern Sonora, Mexico. Salomon Moreno1, Martha Martin1, Fernando Ibarra1, Alejandra Armenta1, Francisco Denogean1, Rafael Retes2, Felix Ayala1, Ernesto Gerlach1 1University of Sonora, Santa Ana, Sonora, Mexico, 2University of Sonora, Hermosillo, Sonora, Mexico Extensive areas of short grasslands in northern Mexico are deteriorated, in poor condition, and show low productivity, reduced vegetation cover and high chances of soil erosion. The study was conducted during 2009 in a Bouteloua-Aristida short grassland at Cananea, Sonora, Mexico to evaluate vegetation changes three summers following the application of biosolids at rates of 0, 10, 20, 30, 40 and 50 ton/ha. Biosolids were hand applied over the soil surface on triplicate 5x5 m plots which were arranged in a randomized complete block design. Variables evaluated were plant density, basal cover, and standing biomass. Data was analyzed by ANOVA (P<0.05). Precipitation was average during the study period. Results show that plant density, cover, and forage production was greater (P<0.05) in biosolid treated plots as compared with the check, even three summer growing seasons after treatment application. Plant density varied from 27.5 to 41.2 p/m2 in treated plots and averaged 19.5 p/m 2 on the checks. Basal cover varied from 18.1 to 37.2% in treated plots and averaged 9.5% on the checks. Forage production varied from 2.5 to 4.3 tons D.M./ha on treated plots and averaged 1.3 tons D.M./ha in the checks. Total standing biomas was 92 to 230% greater (P<0.05) in biosolid treated plots as compared with the untreated checks. We conclude that biosolids play an important role in increasing plant density, cover and forage production on short grasslands in arid and semiarid areas in northern Mexico. They represent a future alternative to restore deteriorated short grasslands. 0181 Groundwater-vegetation relationships in an irrigation-maintained wet meadow and considerations for restoration following irrigation removal Craig Carr1, Tamzen Stringham 2, Erica Freese2 1Montana State University, Bozeman, MT, USA, 2University of Nevada, Reno, Reno, NV, USA Restoring meadow vegetation is a challenge in rangeland systems affected by altered hydrologic regimes. Meadow vegetation is influenced by groundwater depth, thereby providing a management opportunity to manipulate groundwater through strategic meadow flooding or water exclusion. Several studies have evaluated wet meadow restoration associated with increasing groundwater availability, however, fewer studies have evaluated potential changes in vegetation as wet meadows, artificially maintained through irrigation, lose contact with the groundwater when irrigation is foregone and water returned to in-stream use. In 2010, a long-term project was initiated in a formerly irrigated meadow in northwest Nevada to evaluate relationships among vegetation and groundwater and to elucidate the ecological processes driving plant community change. This information will be used to develop an understanding of potential restoration constraints and opportunities and will lead to further research, testing potential treatment options to promote desired ecological processes and vegetation structure. Analyses of 2010 vegetation and depth to groundwater data using non-parametric multivariate techniques indicated 1) differences in vegetation assemblages associated with depth to groundwater; 2) a significant risk of weed invasion associated with propagule pressure and disturbance; and 3) potential state changes in response to altered hydrology. Although early in the study, these data suggested the need to evaluate meadow vegetation response to lowered water table within the framework of state-andtransition models and to consider hydrologic change as a disturbance that may promote invasion by undesirable species. Long-term results of this project may be applicable in other irrigated meadow situations where water use is transferred in-stream. 0186 Comparison of rainfall products of varying spatial scales on herbaceous biomass outputs from the Phytomass Growth Simulator Edward Rhodes1, Doug Tolleson2, Jay Angerer1, Richard Conner3 ,4, Wayne Hamilton3 1Center for Natural Resource Information Technology - Texas AgriLife Research, Temple, TX, USA, 2University of Arizona School of Natural Resources and the Environment, Camp Verde, TX, USA, 3Texas A&M Department of Ecosystem Science and Management, College Station, TX, USA, 4Texas A&M Department of Agricultural Economics, College Station, TX, USA Modeling herbaceous biomass production for forage and fuels-related management requires the proper combination of growth, soil and weather characteristics. The Phytomass Growth Simulator (PHYGROW) is a daily timestep, hydrologically-driven plant growth model requiring accurate, up to date weather data and forecasts. In this project we examined the effects of different NOAA rainfall products of varying spatial scales on PHYGROW model outputs in Arizona. These consisted of the NEXRAD radar (NEXRAD; 4 km resolution), the CPC Morphing (CMORPH; 8 km) and the Unified Precipitation (UNIFIED; 27 km) products. Our study consisted of 3 plant communities located in the Coconino National Forest; desert, juniper, and pinyon. Herbaceous plant communities and soils data were collected in the field and calibrated into the PHYGROW model using NEXRAD data. Model outputs were then recalculated by changing the rainfall data to the CMORPH, and UNIFIED products. The model was considered validated if the output was within the standard error of the clipped field data. All three sites remained calibrated when rainfall data was changed between CMORPH and UNIFIED data. Changing from the 4km to 8km dataset caused the model to lower production on all 3 sites; whereas going from 8km to 27 km triggered production values to increase. The juniper and pinyon sites showed very little difference between the 4km and 27km datasets; whereas the 4km and 8km calibrations were more similar on the desert site. These results suggest that xeric sites may require a higher spatial resolution to yield more accurate model predictions. 0190 Germination of shortgrass prairie species influenced by smoke in the glasshouse. Robert Cox, Yi-Fang Chou, David Wester Texas Tech University, Lubbock, TX, USA Exposure to smoke can influence the germination of seeds in many fire-prone ecosystems, but this effect is not well studied in grasslands. Smoke treatments could be useful as management and restoration tools, if the response of target species in natural settings is well understood. We tested 8 species native to the Southern High Plains region in Texas, USA, already known to respond to smoke in the laboratory, for their response to smoke in a less controlled glasshouse environment. Seeds were exposed to smoke water, heat, or a combination of the two, sown into greenhouse flats, and emergence was observed and recorded. Emergence of nearly all species was influenced by smoke, with most species experiencing either lower emergence or longer times for emergence when exposed to high concentration smoke water. Emergence of Gutierrezia sarothrae seeds was enhanced by smoke exposure, with about twice as many treated seeds emerging as untreated seeds. Because many species displayed different results in the glasshouse as compared to the laboratory, smoke treatments should be tested in the field before being used on a larger scale. Doing so will allow a better understanding of how target species might respond to smoke treatments that interact with soils, fluctuating temperatures, and other complications encountered in the field 0192 Fire seasonality effects on northern mixed prairie production and cover Lance Vermeire ARS, Miles City, MT, USA Seasonal timing is believed to affect plant response to fire. We compared biomass and cover of nonburned, summer (SUM), autumn (AUT), and spring (SPR) burned sites in northern mixed prairie. Criteria for fire timing were summer quiescence, autumn temperature (< -2oC), and spring initiation of Bouteloua gracilis growth. Fire treatments were thrice replicated on silty and dense clay ecological sites monitored two years following fire. All comparisons are relative to non-burned sites. Total current-year biomass was not affected by AUT, reduced 10% by SPR, and reduced 15% by SUM. Fire did not affect grass production (1126 ± 43 kg ha-1). SUM and AUT reduced forb production 65 and 52%. Bare ground increased with SUM (51%) and AUT (33%), but was similar to non-burned sites (12%) following SPR (14 ± 4%). Fire reduced litter cover similarly across fire seasons (55%) compared to no fire (76%) on the silty site, whereas litter cover on dense clay was least for SUM and AUT (34%), greater for SPR (63%), and greatest with no fire (78 ± 4%). Fire increased perennial C3 grass basal cover relative to no fire (0.6%), with SUM (2.8 ± 0.3%) having the greatest effect. Perennial C4 grass basal cover was less with no fire (1.1%) than either SPR (4.3%) or SUM (3.5%) and intermediate for AUT (2.4 ± 0.7%). Grass productivity was resistant to fire and combined cover data indicated native species were promoted by fire in any season (6.6%) compared to sites where fire was excluded (2.0 ± 1.1%). 0193 Influence of Community-based Management and Relative Grazing Pressure on Above-ground Biomass in Three Mongolian Ecoregions Khishigbayar Jamiyansharav, Maria Fernandez-Gimenez, Robin S. Reid, Chantsallkham Jamsranjav Colorado State University, Colorado, USA Mongolia, where the rangeland covers 75-80% of the total territory, has a long history of grazing. About one third of Mongolia’s population is semi-nomadic pastoralists, dependent for their livelihoods on rangeland productivity, which is vulnerable to changing climate and grazing management. Since 1999, over 2000 community-based rangeland management (CBRM) organizations have been established with the aims of improving grazing management and reversing perceived declines in rangeland condition and productivity. We evaluated the effects of CBRM and relative grazing pressure on aboveground biomass in three ecological regions of Mongolia with different ecological dynamics. We compared winter pastures grazed by herders belonging to formal CBRM organizations (n=72) with those grazed by herders in traditional neighborhoods without formal organizations (n=69). Within each winter pasture, we sampled along a grazing gradient from a winter campsite at 100-m, 500-m and 1000-m from the impact point where livestock densities and grazing pressure were assumed to be greatest. We hypothesized that CBRM pastures have enhanced regulation and monitoring of pasture use resulting in greater amounts of above-ground biomass than non-CBRM pastures. We also expected that the effects of relative grazing pressure on above ground biomass would be strongest in the mountain-steppe and weakest in the desert-steppe zone, consistent with the predictions of non-equilibrium rangeland theory. Field observations and preliminary analyses suggest that these data support both our CBRM and nonequilibrium hypotheses. Results of this study have significant implications for policy and management approaches in Mongolia’s diverse rangeland types. 0195 The Potential Value of Festuca valesiaca for Use on Great Basin Rangelands Yingmei Ma1, Jack Staub2, Steven Larson2, Matthew Robbins2, Paul Johnson1 1Utah State University, Logan, UT, USA, 2USDA ARS FRRL, Logan, UT, USA Fine-leaved Festuca valesiaca possesses varied abiotic tolerances and is widely distributed on Asian rangelands. However, its performance in the western United States has not been investigated. Therefore, F. valesiaca accessions were evaluated for performance and their relatedness to species in the F. ovina complex. Twelve F. valesiaca accessions from Kyrgyzstan and eight commercial cultivars were transplanted as spaced plants at Blue Creek, Utah for evaluation. Plant height and width, total biomass, and seed weight and number were evaluated from 2009 to 2011. Amplified fragment length polymorphisms (AFLP) with 10 primer combinations were used to evaluate the genetic relatedness between F. valesiaca and the F. ovina complex. For some traits, F. valesiaca accessions (e.g., PI 659923, PI 659932, W6 30575, and W6 30588) were equal to or better than commercial controls and possessed an abundance of small seeds. Seed production was significantly correlated with total biomass (r2 = 0.84, P = 0.001), plant height (r2 = 0.58, P = 0.05), and plant vigor (r2 = 0.83, P = 0.001). Principal component analysis suggested that these accessions were distinct from the majority of Festuca accessions examined. Neighbor-joining cluster analysis defined four distinct groups: F. idahoensis (Group 1), F. rubra (Group 2), F. ovina and F. valesiaca (Group 3), and F. trachyphylla (Group 4). These relationships were confirmed by genetic structure analysis, indicating that F. valesiaca is closely related to F. ovina. Given their performance, F. valesiaca accessions PI 659923, PI 659932, W6 30575, and W6 30588 should be considered for plant improvement. 0197 Analysis of Beef Cattle Operations in Sonora, Mexico from 2000 to 2009 Francisco G. Denogean B., Salomon Moreno M., Fernando A. Ibarra F., Martha H. Martin R., Felix AyalaA., Rafael Retes L. Universidad de Sonora, Santa Ana, Sonora, Mexico Beef cattle production in rangelands is one of the dominant business operations in Sonora, Mexico. We analyzed statistical information available from various sources regarding the population of cattle in Sonora, Mexico, during the period of 2000 to 2009. We considered the behavior of Sonoran beef cattle herds regarding their number and classification, the characteristics of the production, exports of animals to the United States of America, slaughtered animals, price and value of livestock production, and environmental aspects that influence the production of cattle in Sonora, mainly precipitation and vegetation. Descriptive statistics was used in the analysis using the statistical software SPSS v. 17. Our results indicate that size and herd composition have changed from the previous period of 1991-2000, which reported 1,383,002 head; composed of 49.9% cows, 24.8% calves, 20.6% steers and heifers, and 4.7 % bulls. During 2000 to 2009 the Sonoran herd increased to 1,456,559 head; composed of 53.2% cows, 27.2% calves, 15.8% steers and heifers, and 3.8% bulls. We were able to observe a tendency that ranchers are increasing the number of mother cows, which has in return paid off in larger number of calves, which have had a better price increasing the business profitability. On the other hand, the number of replacement animals, such as heifers, has shown a tendency to decrease in order to maintain more productive cows in the ranch. 0198 Utilizing Winter Grazing to Create Heterogeneity in a Northern Prairie Grassland: Livestock use and Distribution. Christina Koehler, Patricia Johnson, Kent Jensen, Ken Olson, David Gay, Janna Kincheloe, Elise Hughes Berheim South Dakota State University, Brookings, South Dakota, USA Patch-burn-graze systems have been implemented in various areas of the United States to improve range utilization and wildlife habitat. However, in the Northern Great Plains, burning is often viewed negatively. We initiated a study in the winter of 2012 to simulate a patch-burn-graze system with heavy winter grazing used as a replacement for the fire regime. Four native mixed-grass prairie pastures were divided in half, with one half receiving the patch treatment and the other as control. Patches representing twenty percent of the treatment pastures were winter grazed to remove most of the standing vegetation. In mid-May we stocked patch treatment and control pastures at similar stocking rates with yearling steers (253 kg avg. wt.). Two steers in each pasture were fitted with GPS collars to track use and distribution patterns. All cattle were weighed prior-to and after the 10 week grazing season. Parameters evaluated included: (1) vegetation characteristics including height, VOR, percent cover, peak standing crop, and utilization; (2) steer distribution; and (3) livestock responses, including steer weight gains, average daily gains, and livestock production per unit area. Differences in these parameters between treatments were evaluated and changes over the grazing season were also assessed. 0200 The Cost of Increasing Herbaceous Vegetation by Roller Chopping and Seeding in Mixed-Brush Plant Communities Felix Ayala-A.1, J. Alfonso Ortega-S.2, Timothy E. Fulbright2, G. Allen Rasmussen2, D. Lynn Drawe2, Fernando A. Ibarra F.1 1Universidad de Sonora, Santa Ana, Sonora, Mexico, 2Texas A&M University-Kingsville, Kingsville, Texas, USA Rangeland managers commonly assume that seeding is necessary following roller chopping. In semiarid environments, such as south Texas, allowing herbaceous native plant to re-establish from soil seed banks can benefit wildlife as well as domestic animals. Our objectives were to determine if reseeding is required following roller chopping in south Texas mixed-brush communities to reestablish herbaceous plant vegetation; and to evaluate the cost of the increase in herbaceous vegetation cover and species richness with this management practices. The study was conducted on three locations in south Texas. Study sites were selected along an annual precipitation gradient ranging from 550 mm to 920 mm. The experimental design was a randomized complete block with three replications. Treatments evaluated were: roller chopping, roller chopping and seeding a native plant mix, and control. Response variables included canopy cover of woody plants, canopy cover of herbaceous plants and native species richness. Our results indicate that the mechanical brush management practice of roller chopping by itself is enough to reestablish herbaceous vegetation canopy cover; therefore, the cost of seeding after roller chopping is 4 to 8 times higher in order to increase similar herbaceous vegetation cover as with only roller chopping. 0215 Evaluation and Development of Early Successional Native Plant Materials for Rangeland Restoration in Central Texas Mia McCraw1, Forrest Smith1, Jeff Breeden2, Jim Muir3, Randy Bow3 1Texas A&M University Kingsville, Kingsville, Texas, USA, 2Tarleton State University, Stephenville, Texas, USA, 3AgriLife Research and Extension, Stephenville, Texas, USA The success of a native seeding project often hinges on local adaptability of plant materials utilized and diversity of successional stages of plants within the seed mix. Often the commercial market for certified native seed is dominated by most desirable late successional species that provide the best forage and cover. However, slow germination and poor success rates of current seeding projects suggest successful establishment of these late successional species requires site modifications created by early to mid successional species often not available in commercial seed trade. To better understand statewide adaptability of available seed sources, the Texas Native Seeds project is conducting field trials of 65 commonly used native and improved species in Central Texas at the Stephenville AgriLife Research field and the Knox City NRCS Plant Material Center. Seeding trials and transplants at each location are being evaluated for many variables including germination, vigor, cover, and long-term adaptation. Data collected over the past two years supports development of early successional native plant materials for use in seeding projects. White tridens (Tridens albescens) and purple three awn (Aristida purpurea) are two such species we are working to make commercial seed selections of. In summer 2012, we began evaluations of 70 native populations of white tridens and 93 native populations of threeawn for selection and increase purposes. Ultimately, the best performing collections will be increased, and provided to commercial growers for certified production. Increasing the availability of certified seed of early successional species should improve native seeding success in Central Texas. 0216 Evaluating Weed Management Options to Improve Reclamation of Drastically Disturbed Lands Beth Fowers, Brian Mealor University of Wyoming, Laramie, WY, USA Reclamation after disturbance of soils and vegetation associated with energy extraction is critical for ecosystem function and required by law. Soils are scraped from sites to access mineral deposits or to create level surfaces for drilling or mining activities. This disturbance kills plants, and makes it necessary to reintroduce desirable species once extraction activities are completed. Weedy annual species often dominate reclamation sites for the short-term, competing for resources with newly-seeded desirable vegetation. Our objectives are to: 1) evaluate herbicide effects on weedy and desirable species, 2) determine the effect of treatment timing (herbicide and seeding) on reclamation success, and 3) evaluate the performance of different species and seed mixes. Fifteen herbicide treatments and ten seed mixes were applied in a split-plot design to three sites in Wyoming to evaluate effectiveness of reclamation practices. Most herbicide treatments targeted broadleaved weeds with the addition of two treatments targeting annual grasses at one of the three sites. Seeding treatments were drill seeded at two timings (fall, spring) across herbicide treatments to investigate establishment rates of specific species under different seeding time treatments. We present first-year observations on weed control and seeded species establishment from cover and percent stand observations from mid-summer 2012. All herbicide treatments reduced annual weedy forb cover (p<0.05) across the three sites. Overall, seeded species establishment was low, presumably because of very low precipitation in 2012. Current results are too early in the reclamation process to make reliable management recommendations, but evaluation will continue for two more years. 0217 Potential Direct and Indirect Effects of Climate Change on Northern Great Plains Grasslands David King1, Dominique Bachelet1 ,2, Amy Symstad3 1Oregon State University, Corvallis, OR, USA, 2Conservation Biology Institute, Corvallis, OR, USA, 3U.S. Geological Survey, Hot Springs, SD, USA Climate affects grassland production and composition directly and quickly by governing soil moisture resources, as well as indirectly and more slowly by influencing management decisions regarding grazing and fire. Using MC1, a dynamic global vegetation model that incorporates carbon dioxide effects, climate, fire, and grazing, we are simulating vegetation dynamics under a combination of future climate and management scenarios for United States northern Great Plains (US NGP). Simulations for Wind Cave National Park (WCNP), a 13,000-ha natural area with bison grazing on a prairie-forest transition in the southern portion of the US NGP, show that simulated future vegetation is quite sensitive to fire management. Woody species spread into nearly all park grasslands in a fire suppression scenario but remain in currently forested areas in a "natural" fire scenario. These WCNP simulations suggest that fire management will become more difficult as increasing temperatures and decreasing humidity increase the frequency of high fire danger days from approximately 10 per year in the 20th century to 20, 60, or 100 days per year by 2100 for three contrasting climate scenarios. Simulated grass production for two of the climate scenarios remains similar to current levels well into the 21st century when grazing is moderate, but in the hottest, driest scenario grass production decreases by at least 30% from its current level by 2100. With this poster, we seek feedback from SRM members on realistic grazing and fire scenarios to apply to our simulations for all of the US NGP. 0219 Effects of sequential herbicide applications on cheatgrass and native rangeland vegetation Shayla Burnett, Brian Mealor University of Wyoming, Laramie, WY, USA Cheatgrass (Bromus tectorumL.) is one of the most significant invasive weeds of North America. Because eradication may not be a feasible goal, it may be desirable to manage cheatgrass in low-density stands below the ‘impact’ stage of invasion. Little work has investigated managing such stands by single or sequential herbicide applications. We inset randomized complete block experiments with five herbicide treatments and a control into seven different sites. Five sites were treated previously with imazapic in different years (2006-2010). Two sites – one dominated by Hesperostipa comata (Trin.&Rupr.) and one co-dominated by Pascopyrum smithii (Rydb.) and Bouteloua gracilis (Willd.exKunth) – were treated for the first time in 2011. Herbicide treatments included imazapic (70 and 105 g ai/ha), propoxycarbazone sodium (59 g ai/ha), rimsulfuron (158 g ai/ha), and a combination of tebuthiuron (170 g ai/ha) and aminopyralid (92 g ai/ha) applied prior to cheatgrass emergence in autumn 2011. Vegetation response (cover and biomass production) was evaluated spring 2012. Cheatgrass cover was observed only at the site initially treated in 2006 and at the sites untreated previously, indicating potential cheatgrass reinvasion at the 2006 site. All herbicide treatments reduced cheatgrass biomass (p<.0001) and increased perennial grass biomass (p=.0379) at the 2011 H. comata site but had little effect on vegetation cover at the other sites (p>0.05). Although treated repeatedly with herbicide, desirable natives did not show significant damage – even in a drought year. Further work includes evaluating the seedbank changes at all sites and grazing exclusion at the 2011 sites. 0221 Effects of Forest Harvesting, Wildlife Browsing and Cattle Grazing on Understory Vegetation and Lodgepole Pine Regeneration: Fallen Timber Coniferous Regeneration Study Summary Report Terri France, Carcey Hincz, Michael Willoughby Government of Alberta Environment and Sustainable Resource Development, Alberta, Canada In Alberta, public lands are managed to provide economic and ecological goods and services to Albertans through the sustainable use of natural resources. However, integrating a variety of activities and multiple uses on the landbase can be challenging and adaptive management is often required. Multiple-use goals for cutblocks include successful forest regeneration, sustained timber yields, and maintenance of forage for livestock and wildlife. The Fallen Timber study investigated the effects of cattle grazing, wildlife browsing and forest harvesting practices on lodgepole pine regeneration and understory vegetation. Three monitoring sites were established in clearcut harvested cutblocks within a grazing lease located in west-central Alberta, Canada. The objectives of the study were to compare grazing treatment, slash treatment, and their interaction on forage production, species composition, and tree regeneration. At each site six treatment combinations were examined which included three grazing treatments (unrestricted grazing, cattle excluded, and cattle and wildlife excluded) and two slash treatments (trees limbed in block and trees limbed at landing). The effects of the grazing treatments on forage production were not significant; whereas, the presence of slash in the block significantly reduced forage production. Species composition did not significantly differ among the grazing treatments or slash treatments. The grazing treatment by slash treatment interaction was also not significant. Lastly, lodgepole pine seedling survival was not significantly affected by grazing treatment or slash treatment. These results are consistent with most studies that show cattle grazing can be compatible with timely free to grow coniferous regeneration standards. 0223 Ecological responses to cattle grazing on a degraded southeastern Arizona mine site Shawn Stone University of Arizona, Tucson, Arizona, USA There has been widespread debate regarding the use of grazing as a tool in ecosystem reclamation. Some parties believe that grazing by domestic livestock threatens the structure and function of natural ecosystems, as it may increase soil compaction and deformation, reduce plant density and biodiversity, increase runoff and soil erosion, and export minerals from the system. However, some studies have posited that short-duration grazing may increase production of some grasses by increasing water infiltration, incorporation of litter into the soil, and root growth. This study aims to improve our understanding of these processes in arid and semi-arid areas. We introduced grazing to an existing reclamation experiment at the Rosemont Copper property in southeastern Arizona. There are two test sites at differing elevations, each containing twenty-four plots that were broadcast-seeded with ten native desert grassland species. Within each plot, we constructed exclosures to limit the impact of grazing to a defined area. The cattle were introduced after each growing season and were managed for short-term, low-intensity grazing on each site. We then evaluated the differences in vegetative response, macronutrient loads, microbial communities, and slope stability between the exclosed and open areas, and then compared those results to previous, ungrazed years’ results. Our results may provide a novel reference for the use of grazing as a tool in other desert grassland reclamation projects. 0226 Timing and Intensity of Steer Use on Old World Bluestem (Bothriochloa ischaemum) and Blue Grama (Bouteloua gracilis) in Southern Mixed-Grass Prairie Jessica Casey1, Jordana LaFantasie1, Keith Harmoney2 1Fort Hays State University, Department of Biological Sciences, Hays, Ks, USA, 2Kansas State University Agricultural Research Center-Hays, Hays, Ks, USA Invasive grasses are of particular concern when it comes to native grassland communities; the invasive grasses decrease the productivity of the native grasses in the native grasslands. Old World Bluestems are an increasing problem in many of the prairie states in North America. Yellow bluestem is a non-native species that has been introduced for hay production, pasture grasses, and roadside grasses for erosion control, and has now exceeded the bounds. Cattle have been known to graze yellow bluestem early in the grazing season along with the cool season grasses, and then the cattle begin to graze native warm season grasses. The objectives of this study are 1) compare timing and intensity of defoliation of yellow bluestem and native grass, blue grama by steers, and 2) determine whether mowing pastures with yellow bluestem will increase the amount of steer use. I hypothesized that 1) yellow bluestem and blue grama will be utilized the by steers, 2) the recently mowed areas will have higher defoliation rates than the unmowed areas due to the fact that the standing dead tissue would discourage steer use will be removed, and 3) blue grama will have a significantly higher rate of defoliation than the yellow bluestem. Some preliminary results are both blue grama and yellow bluestem were utilized by the steers. The yellow bluestem was defoliated at higher intensities in the mowed areas than in the un-mowed areas. 0235 Habitat selection patterns of young cows in grassland/woodland mosaics in relation to stocking rate and weather Mohammed Sawalhah1, Andres Cibils1, Caitriana Steele1, Jerry Holechek1, Robert Wesley1, Christina Black1, Shad Cox2, Richard Dunlap2 1New Mexico State University, Las Cruces, NM, USA, 2Corona Range and Livestock Research Center, Corona, NM, USA A study was conducted in central NM to evaluate effects of two levels of stocking rate and weather factors on habitat selection (grassland vs. woodland) of young rangeland raised cows grazing a grassland/woodland mosaic. Data were collected over four years by tracking 52 cross-bred cows grazing a 146 ha pasture (55% grassland and 45% woodland). GPS collars were used to record and store cow position at 5-min intervals. The pasture was stocked moderately in 2004 (73 AUMs) and 2005 (78 AUMs) and lightly in 2006 (34 AUMs) and 2007 (32 AUMs). A preference index which computed the ratio between percentages of time spent in the woodland by cows and percentage of woodland area in the pasture was used as the main response variable. Stocking rate level significantly (P<0.01) affected daily woodland preference during the entire 24h day + night period (D+N), daytime hours (DAY, from sunrise to sunset), pre-night hours (PRE, from midnight to sunrise), and post-night hours (POST, from sunset to midnight). During all four time periods, cows in moderately grazed treatment spent more time in woodland area (higher preference index) than counterparts in lightly grazed treatment (P<0.01). Cumulative precipitation, wind speed or direction, and air temperature affected woodland preference regardless of stocking rate. Lunar cycle affected the time spent in woodland area during D+N (P<0.05) and DAY (P<0.01). Previous studies indicate that woodland preference increased during the years when forage availability was scarce in the grassland open area. Our results support this hypothesis. 0239 Effect of landscape position on soil carbon and nitrogen in three different arid and semi-arid ecosystems. Doug Tolleson1, Jay Angerer2, Stephen Prince2, Marco Bomfim2 1The University of Arizona, School of Natural Resources and the Environment, V Bar V Ranch, Camp Verde, AZ, USA, 2Texas AgriLife Research, Center for Natural Resource Information Technology, Blackland Research and Extension Center, Temple, TX, USA We analyzed the effect of landscape position on soil carbon and nitrogen content in three different ecosystems in the southwestern US (MLRA's 38, 41, and 42) in spring 2012. Within each experimental location, soil surface (< ~2.5 cm) samples were collected from two different ecosystem sites and within each site; from under shrub canopy, grass canopy, and interspaces. Ecosystem sites, shrub, and grass species varied and were specific to each experimental location. Samples were analyzed for total carbon and nitrogen. Differences in soil nutrients were determined by Mixed Model procedures in SAS. Results are reported as least square means ± standard error. Ecosystem sites within experimental locations generally differed in soil nutrients. The effect of landscape position on soil surface carbon and nitrogen was not consistent across experimental locations. In MLRA 38, soil under shrubs had greater (P < 0.02) percent carbon and nitrogen (2.44 ± 0.35, 0.23 ± 0.02, respectively) than under grass (1.35 ± 0.35, 0.17 ± 0.02) or within interspaces (0.81 ± 0.35, 0.13 ± 0.02). The same effect of landscape position was true (P ~ 0.1) for the MLRA 41 location; i.e. shrub (1.00 ± 0.25, 0.13 ± 0.03) > grass (0.37 ± 0.25, 0.07 ± 0.03) > interspace (0.18 ± 0.25, 0.06 ± 0.03). In the MLRA 42 location, however, shrub (5.83 ± 0.40, 0.30 ± 0.02) > interspace (4.50 ± 0.40, 0.22 ± 0.02) > grass (3.51 ± 0.40, 0.17 ± 0.02; P < 0.01). Landscape position and site affected soil carbon and nitrogen. 0247 EFFECTS OF SOIL HETEROGENEITY ON WOODY PLANT ENCROACHMENT IN THE ARGENTINE CALDENAL REGION: A CLASSIFICATION APPROACH Lauren Svejcar1 ,2, Brandon Bestelmeyer2, Raul Peinetti3 1New Mexico State University, Las Cruces, NM, USA, 2USDA ARS Jornada Experimental Range, Las Cruces, NM, USA, 3Universidad Nacional de La Pampa, Santa Rosa, La Pampa, Argentina In the Caldenal ecoregion of central Argentina thicketization of the calden trees (Prosopis caldenia burk) in a previously savanna ecosystem resulted from a change in grazing history and altered fire frequencies. The transition from savanna state to thicketized state has had negative effects on ecosystem services, such as loss of palatable species, and has advanced the system's level of ecological degradation with increased erosion being a primary concern. Little success has been achieved through attempts to minimize losses of ecosystem services and restore historic savanna states by reduced grazing and prescribed burning. In this project, we drew together existing sources of information to develop ecological site classifications with correlated, process-oriented state-and-transition models (STM), use measurements of vegetation and soils collected with both random and selected sampling schemes to examine state concepts and characterize the properties of alternative states, and then ask how the occurrence of thicketized states may be related to variations in soil properties. We sought to examine the relationship of vegetation structure to selected dynamic soil properties that reflect the functioning of the states, including texture, P, N, soil organic matter (SOM), calcium carbonate content of soil horizons and depth to petrocalcic horizon. We hypothesized that thicketized states would exhibit increases in total P, total N and SOM compared to ancestral savanna states. The information derived from this project can serve as a basis for recognizing different ecological sites bearing specific state-and-transition models that can assist land managers in land use decisions. 0248 Phenological bud bank development of Bouteloua gracilis, Hesperostipa comata, and Pascopyrum smithii during drought in the northern Great Plains Morgan Russell1, Lance Vermeire2, John Hendrickson3, Amy Ganguli4 1North Dakota State University, Fargo, ND, USA, 2USDA-ARS, Miles City, MT, USA, 3USDA-ARS, Mandan, ND, USA, 4New Mexico State University, Las Cruces, NM, USA Vegetative reproduction in rangelands relies heavily on tiller recruitment from belowground bud banks. Improved understanding of species-specific bud production and phenology would facilitate timing of aboveground management strategies. Tillers from twelve individual plants of the warm season grass (Bouteloua gracilis), and cool season grasses Hesperostipa comata, and Pascopyrum smithii were marked on 15th of March and followed throughout the 2012 growing season. Tiller counts, mean growth stages of tillers, and axillary bud production of marked plants were recorded bi-weekly. Mean growthstage counts (MSC) estimate the average growth stage of the tiller populations. Phenological development of H. comata and P. smithii differed during the first sampling, but demonstrated a similar MSC by the second sampling. Pascopyrum smithii matured earlier than H. comata and was approximately 2 weeks ahead until 30th July when both species reached a developmental plateau. Both cool season grasses produced the most active buds by mid-March, with H. comata producing 3.3 ± 0.4 and P. smithii producing 7.2 ± 0.4. In contrast, B. gracilis initiated maturation at the end of May and experienced rapid phenological development during the first week in July producing the most active buds (6.7 ± 0.4) by 5th July and decreased active bud production by 16th July (4.8 ± 0.4). Abrupt decreases in B. gracilis active buds may be attributed to drought. Better understanding of bud phenology may improve predictability of grassland responses to timing of various stimuli, such as fire, grazing or drought and their effects on tiller and biomass production. 0249 Impact of burning and glyphosate on restoration success in a crested wheatgrass stand. John Hendrickson USDA-ARS, Mandan, ND 58554, USA We investigated the ability of fire and/or glyphosate to increase seeding success of native grasses in an established crested wheatgrass monoculture [Agropyron cristatum (L.) Gaetn.] on US Forest Service land in Perkins County South Dakota. Treatments were 1) burn only; 2) burning and seeding; 3) burning seeding and followed by a glyphosate application; 4) no burning but seeding 5) no burning but seeding followed by glyphosate and 6) an untreated control. Each treatment was applied to a 30 x 20 m plots and replicated four times. Plots were burned on April 17, 2008 seeded on May 12, 2008 and sprayed on May 20, 2008. The seeding mixture consisted six native grasses dominated by western wheatgrass [Pascopyrum smithii (Rydb.) Á. Löve ] and green needlegrass [Nassella viridula (Trin.) Barkworth]. Glyphosate was applied at the rate of 13 oz a.i/acre. One thousand points were collected in each plot using 10 point frames to determine species composition in late July of 2008, 2009 and 2011. Basal hits were divided into native grasses, native forbs, introduced grasses, introduced forbs and moss. Our data indicate burning did not increase the percent native grasses or forbs in the species composition. However, glyphosate nearly doubled the percent of native grass or native forbs compared to the controls. Burning or spraying reduced moss compared to non-treated plots. Our data suggests that the use of glyphosate, with or without burning, is an effective treatment to increase native grass seeding success in crested wheatgrass stands. 0250 Soil Microbial Community Responses to Grazing Period Length and Stocking Density in Nebraska Sandhills Meadow Robert Vavala, Walter Schacht, Jerry Volesky, Rhae Drijber, Elizabeth Jeske University of Nebraska-Lincoln, Lincoln, NE, USA Soil health is important for long-term grassland ecosystem structure and function. A diverse microbial community is generally linked with healthy soil as its activities can increase soil fertility, improve soil structure, decrease nutrient leaching, and improve suppression of diseases. Analysis of fatty acid methyl ester (FAME) profiles within soil can be used to determine the main groups of microorganisms present in the soil and their relative abundance. The purpose of this two-year study was to investigate how grazing period length and stocking density, either directly or indirectly, affected soil microbial function on a subirrigated meadow in the Nebraska Sandhills. One treatment entailed grazing 36 yearling steers on 0.06 ha for one half day for a stocking density of 445 animal units (AU) per hectare. The second treatment used four steers grazing continuously on 0.75 hectares for a stocking rate of 4.25 AU per hectare. Two replications of each treatment and two ungrazed controls were included. For each sampling date, fifteen six-inch soil cores were taken from each treatment paddock and composited. Samples were taken one week and one day prior to grazing, as well as, one day, one week, one month, and two months after grazing began. FAME analysis was performed on all composited samples. Results of analysis will be presented. 0253 Sensitive species range mapping in Wyoming Joy Handley, Mark Andersen Wyoming Natural Diversity Database, Wyoming, USA Wyoming Natural Diversity Database (WYNDD) is the Natural Heritage program in Wyoming and offers the most complete source of data for species and vegetation communities of conservation concern in Wyoming. Part of the WYNDD mission is to distribute these data upon request under the philosophy that the best decisions regarding natural resources will be made only when everyone has access to complete and current scientific data (http://www.uwyo.edu/wyndd/). Recently, the online application Wyoming Interagency Spatial Database & Online Management System (WISDOM, http://wisdom.wygisc.org/) was developed to allow users to determine species of concern that may be present within a specific area of interest without requiring GIS experience or software. Range data provide a useful visual representation for understanding the geography of a taxon at broad scales. In order to provide clear range mapping for species, it was decided to use Hydrologic Unit Code (HUC10 or watershed) Boundaries, rather than "dot maps" of known species observations. Watersheds are accessible as a consistent dataset for the entire United States, and can be produced at finer and coarser scales at different levels. Pinus albicaulis (whitebark pine) and Pinus flexilis (limber pine) are declining due to Cronartium ribicola (white pine blister rust), Dendroctonus ponderosae (mountain pine beetle), and other environmental changes. Both these pine species have important ecological and ethnobotanical roles in their high elevation habitats. WYNDD recently started tracking Pinus albicaulis and Pinus flexilis as species of potential concern, and the maps of these two species are useful examples of range mapping and WISDOM capabilities. 0257 Influence of temperature and precipitation on steer gains in Northern Mixed-Grass Prairie Justin Reeves1, Justin Derner1, Matt Sanderson2, Mark Petersen3, Matthew Rinella3, John Hendrickson2, Scott Kronberg2, Lance Vermeire3 1USDA - ARS, Cheyenne, WY, USA, 2USDA - ARS, Mandan, ND, USA, 3USDA - ARS, Miles City, MT, USA Precipitation and temperature influence forage production in Northern Mixed-Grass Prairie, but it is unclear if these environmental variables also influence livestock production. Yearling steer gains and weather data have been collected at the USDA-ARS, High Plains Grasslands Research Station near Cheyenne, WY since 1982. Using these long-term data and novel model averaging methods, we determined responses of average daily gain (kg/hd/day) and beef production (kg/ha) to stocking rate, precipitation and temperature in individual spring months (April, May, June) and combined summer months (July - September). Prior fall/winter (prior October to February) and prior growing season (prior April - prior September) precipitation was examined as well, along with multiple interaction terms. Though stocking rate alone explained more variability than any of the climate parameters (~17% of daily gain variability; ~76% of beef production variability), addition of the climate parameters above increased the predictive ability of the models to ~57% of daily gain variability and ~84% of total production variability. Incorporation of relationships between stocking rate and the above climate variables on steer gains into decision support tools will assist in the reduction of risk associated with running yearlings in the highly variable climate of the Northern Mixed-Grass Prairie. 0258 Influence of climatic variability on livestock gains in the Northern Great Plains: a collaborative effort by the USDA-Agricultural Research Service Justin Reeves1, Justin Derner1, Matt Sanderson2, Mark Petersen3, Matthew Rinella3, John Hendrickson2, Scott Kronberg2, Lance Vermeire3 1USDA - ARS, Cheyenne, WY, USA, 2USDA - ARS, Mandan, ND, USA, 3USDA - ARS, Miles City, MT, USA Understanding how climate change (i.e., variability of precipitation and increasing temperatures) affects livestock production is critical for ensuring food security and maintaining economic viability of rural communities. Relationships for determining forage production from precipitation and temperature have been used by land managers to reduce risk, but similar efforts for livestock weight gains are needed. To evaluate relationships with livestock gains, we will use existing long-term (30-94 years) data sets from three USDA-Agricultural Research Service (ARS) locations in the Northern Great Plains (Cheyenne, WY; Miles City, MT; Mandan, ND),where livestock production is a key agricultural enterprise. The central question asked here is: How does climatic variability influence livestock weight gains in the northern Great Plains? Our hypotheses are that livestock weight gains are positively influenced by amounts of cool-season (spring) and warm-season (summer) precipitation in the northern Great Plains and that monthly average temperatures during the grazing season do not affect livestock weight gains. Relationships developed for each site based on these unprecedented analyses, along with a synthetic relationship across these locations, will be incorporated into decision support tools that reduce risk and facilitate adaptive management decisions for land managers. 0263 Predicting forage mass for cereal forages with a rising plate meter Corey Moffet, Ryan Reuter, James Rogers The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA Wheat and rye are important forages for beef stocker operations in Oklahoma. Accurate forage mass measurements are needed to set stocking rates. Rising plate meters have been used to rapidly estimate forage mass. The objective of this study was to develop equations to estimate cereal forage mass with a rising plate meter. We collected 20 datasets with paired measurements of plate height and clipped mass between 15 November, 2011 and 20 April, 2012. A linear mixed model was used to investigated the effect of rye or wheat, no-till or conventional till, and days since a typical 1 September seeding date on the relationship between mass and plate height. Only days since seeding effected the relationship. The standard deviation of the random intercept for dataset was 455 kg/ha. The standard error of an estimate from the population level model includes the random effect and residual error. In practice, a pasture forage mass is estimated from the mean of many samples. With 30 and 60 height measurements (average height = 12.7 cm, sd = 2.5 cm) and a population level model, users can predict forage mass with sd = 490 and 481 kg/ha, respectively. With the dataset specific model, the sd is reduced to 145 and 103 kg/ha, respectively, with only a small change in the absolute estimate (-35 kg/ha). If relatively low precision of forage mass estimates are acceptable, the plate meter is a very efficient method. Greater precision of estimates requires a dataset-specific model, which requires additional calibration effort. 0264 An experimental assessment of different restoration practices in a brush encroached South Texas rangeland Carissa L Wonkka1, William E Rogers1, Dirac Twidwell2 1Texas A&M University, College Station, TX, USA, 2Oklahoma State University, College Station, TX, USA Woody plant encroachment into semiarid rangelands is a worldwide phenomenon that is having negative consequences for a variety of important ecosystem services. We designed a study to experimentally determine the effects of several common brush removal techniques for restoration of south Texas rangelands. At each of three sites in Zavala county Texas, 18 (0.15 acre) plots were established across three soil types with treatments randomly assigned within soil blocks. Mechanical brush removal treatments were initiated in fall 2010 and prescribed fires conducted in fall 2012 once sufficient surface fuels accumulated in each treatment area. The treatments are as follows: 1. control (no mechanical removal and unburned), 2. burned (no mechanical removal), 3. cut-stump (cut and treated with herbicide and unburned), 4. cut-stump and burned, 5. roller-chop (unburned), 6. roller-chop and burned. Woody brush and herbaceous cover were estimated pre- and post-treatment for the entire unit, and individual grasses were mapped in five 1m 2 quadrats randomly located with each larger experimental plot. One year following initiation of mechanical treatments, woody cover was substantially reduced in cut-stump and roller-chopped plots compared to controls, as expected. However, resprouting occurred in both mechanical treatments. Additionally, grass cover was substantially reduced in roller-chopped plots compared with cut-stump and control plots. Herbaceous cover took two years to recover enough to initiate prescribed fire in the roller-chopped plots, although extreme drought conditions in 2011 may have exacerbated this effect. Additional data are needed to determine the relative effectiveness of these treatments for restoring woody encroached rangeland ecosystems. 0267 Riparian Ecological Site Descriptions in northern Utah: a collaborative project with the BLM Sarah Quistberg1, Mellissa Rosenhan2 1USDA-NRCS, Ogden, UT, USA, 2USDOI-BLM, Salt Lake City, UT, USA Riparian ecological sites are designed to address the ecotone between moving water and the adjacent uplands. Riparian ESD's do not fit neatly into the rangeland ESD model of single or multiple soils with the potential of producing one type of plant community. Riparian complex ecological sites allow multiple plant communities with generally occur parallel to the stream channel. Developing riparian ecological sites includes inventory of the multiple plant communities and soils that occur within the riparian zone and describing the stream and hydrology of the system. Utah has been involved in developing riparian complex ecological sites for the northeastern part of the state in collaboration with the Bureau of Land Management. We have developed four ecological sites on BLM land using some existing data and data we collected. The riparian ESD's can be used for watershed or allotment management. 0269 Influence of Extreme Precipitation Events on Livestock Production in Shortgrass Steppe Justin Derner1, David Augustine1, Daniel Milchunas0 ,2 1USDA-Agricultural Research Service, Cheyenne, WY/Fort Collins, CO, USA, 2Colorado State University, Fort Collins, CO, USA The influence of extreme precipitation events on livestock production has substantial implications for sustainably providing animal protein for a growing world population. Here, we used a long-term (19392008) dataset of precipitation and yearling Hereford heifer weights to assess the influence of extremely dry and wet periods (lowest and highest 10% of years), compared to "normal" (middle 10% years), during the spring (April-June), summer (July-September), prior fall/winter (prior October to March) and prior growing season (prior April to prior September) on livestock production. Pastures were grazed at moderate (40%) or heavy (60%) intensities from May-October. Compensation occurred with increased beef production (kg/ha) occuring with wet spring years (21% in heavy, 9% in moderate) overcoming reductions of 9% (heavy) and 1% (moderate) in dry springs. Compensation also occurred for beef production in moderate grazing with summer precipitation (+15% increase in wet and 4% decrease in dry). In contrast compensation for beef production did not occur for heavy grazing with summer precipitation (+2% increase in wet compared to 20% decrease in dry), and for both grazing intensities with fall/winter and prior growing season precipitation. These findings suggest that more frequent extreme precipitation events in all seasons, except spring, will further increase risks and decrease sustainability of livestock production for managers employing heavy compared to moderate grazing intensities. 0271 Forage Quality of Selected Grasses in the Lincoln Hills of Missouri Allen Casey, Steve Bruckerhoff, Ron Cordsiemon USDA-NRCS-Plant Materials Center, Elsberry, Missouri, USA The changes in forage quality of some grasses throughout the growing season have not been well documented in the Eastern United States. In recent years there has been a push in the Eastern United States to convert cool-season grass pastures to perennial warm season grasses. There is limited data on the change in forage quality throughout the growing season and its relation to beef production. In 2002 accessions of ‘Rountree' big bluestem (Andropogon gerardii Vitman), 9078831 big bluestem, ‘Cave-InRock' switchgrass (Panicum virgatum L.), 9062244 switchgrass, ‘Aldous' little bluestem (Schizachyrium scoparium Nash), ‘Rumsey' Indiangrass (Sorghastrum nutans Nash), 9083214 Eastern gamagrass (Tripsacum dactyloides L.), ‘Pete' Eastern gamagrass, ‘El Reno' sideoats grama (Bouteloua curtipendula Torr.), and ‘Ozarka' bermudagrass (Cynodon dactylon Pers.) were grown in a replicated complete block design. Vegetative samples of primary and re-growth of the selected species were taken at multiple times throughout the growing season and tested for forage quality. The acid detergent fiber (ADF) varied from 27.2% to 43.2%, the neutral detergent fiber (NDF) varied from 46.4% to 68.5%, and total digestible nutrients (TDN) varied from 49.2% to 60.6% for all species tested throughout the growing season. The selected species have the potential to provide adequate to excellent forage quality but proper management is needed to maintain that quality. 0272 Utilizing NAIP Imagery to Estimate Tree Cover and Biomass in Pinyon and Juniper Woodlands April Hulet1, Bruce Roundy2, Steven Petersen2, Stephen Bunting3 1Agricultural Research Service, Burns, OR, USA, 2Brigham Young University, Provo, UT, USA, 3University of Idaho, Moscow, ID, USA Land managers need to be able to rapidly assess and monitor fuels in pinyon and juniper (PJ) woodlands. Geospatial technologies, particularly remote sensing, could potentially be used in these ecosystems to better understand the spatial distribution of fuels and monitor PJ expansion at a scale necessary to make management decisions. This research utilized the National Agriculture Imagery Program (NAIP) aerial imagery to assess the relationship between remotely sensed tree cover and ground based cover and biomass measurements collected as part of the Sagebrush Steppe Treatment Evaluation Project (SageSTEP). eCognition Developer software was used to extract tree cover from imagery using objectbased image analysis (OBIA) techniques. Ground measurements were collected during the summer of 2006 in 30x33-m subplots using the crown diameter method; biomass was estimated using structurally based analytic models. Averaged across all sites, OBIA cover estimates were approximately 2.5% less than ground-measured cover. Cover estimates from the two methods were highly correlated (r = 0.91) suggesting that NAIP imagery and OBIA techniques are a good method to rapidly identify areas threatened by PJ expansion. Preliminary correlation results between OBIA cover estimates and groundmeasured biomass are highly correlated for Utah juniper trees (r = 0.87). However, for western juniper trees the correlation is less (r = 0.57), which may be due to structural differences between juniper species which impacts both our OBIA cover estimates and ground-measured biomass 0273 Economic Diversification of Farms in Namibia, Africa: Can Ecotourism and Fee Hunting Programs find Application in the Great Plains Laura Snell University of Nebraska- Lincoln, Lincoln, NE, USA Drought and increasing land and cattle prices may decrease the profitability of family farms and ranches in the Great Plains, and one solution is to diversify farm income through tourism and fee hunting. Much can be learned from farmers in Namibia, Africa who have already developed these enterprises on their land in an effort to diversify their farm incomes and cope in years when traditional agriculture and cattle ranching is not profitable. Only two percent of Namibia has suitable rainfall for crop production, but over 70% of the 825,000 km2 country is used for grazing livestock. In June and July of 2012 three farms were studied in three different geographic regions of Namibia. Survey data was gathered by interviewing and shadowing three landowners, as well as, interviewing 16 of their clients. Along with economic benefits, several social and cultural benefits were documented. A majority of the interviewed farmers and clients supported further development of fee hunting programs and ecotourism in the Great Plains and were honored that the Namibian system might be used as a model for the United States. Data gathered on the development and daily operations of the three Namibian farms will provide information to aid farmers in the Great Plains to develop profitable and long-lasting ecotourism operations. 0274 Grasshopper herbivory reduces blue grama abundance in a northern mixed grassland David Branson USDA, Agricultural Research Service, Sidney, MT, USA Insect herbivores often comprise more biomass than vertebrate herbivores in grassland ecosystems, but little longer term research has examined insect herbivore impacts on plant communities in grass dominated ecosystems. A manipulative cage-based experiment was conducted from 2000 through 2006 at a northern mixed-grass prairie site in eastern Montana, USA. Treatments consisted of vegetation controls, Ageneotettix deorum herbivory (whitewhiskered grasshopper), and Melanoplus sanguinipes herbivory (migratory grasshopper). Vegetation composition was monitored using a 100 point frame in early summer prior to installation of cages. Blue grama declined in abundance by the end of the six year experiment with the gramnivorous feeding A. deorum, but was not affected by a larger bodied mixed grass and forb feeding grasshopper M. sanguinipes. Grasshoppers were maintained at similar densities that did not strongly reduce plant biomass. The results were likely driven by plant preference, as A. deorum selected grasses more in proportion to their abundance and M. sanguinipes selected against blue grama. Although blue grama could be less resistant to herbivory by a grass feeding grasshopper than cattle, additional research on a wider range of grasshopper species is required. The results point to the need for additional future research on the impact of abundant grasshopper herbivores on rangeland health and vegetation composition, in light of grasshoppers’ frequent dominance by biomass in grassland systems. 0275 Improving Sage Grouse Habitat from a Watershed Perspective Tammy Koldyke0, Patti Barney0 ,2 1Mule Deer Foundation, Ogden, UT, USA, 2USDA-NRCS, Ogden, UT, USA In 2010, the USFWS gave sage grouse the designation of "warranted but precluded" under ESA. That means that the science shows these birds qualify to be listed & protected, but that higher priority species come first. The designation gives partners across the west extra time to take action to prevent the need for listing. This poster will reflect the work that has been done within watershed areas to affect sage grouse habitat through working with private landowners, public land agencies, and many other partners in Northern Utah. It will also demonstrate the collaborative process used to effectively work with varous entities. 0278 Soil Temperature and Available Soil Water Characterization of Sagebrush Steppe Seedbeds in the Great Basin Nathan Cline, Bruce Roundy Brigham Young University, Provo, UT, USA Practical application for predicting germination of weed and revegetation species from thermal-time models (summation of heat units over time) depends on the amount of time wet seedbeds (> -1.5 MPa) are at various field temperatures. From the literature, thermal-time models predict germination at moderate temperatures (5-30°C) fairly well (R2> 0.7). For extreme temperature ranges (<5°C and >30°C), thermal-time models have poor statistical fit. The objective of this study was to quantify wet seedbed duration at moderate and extreme temperature ranges. Also, the effect of site, season, year, and treatment were tested in order to better understand the variations in the seedbed environment. Using thermocouples and gypsum blocks, near-surface (1-3 cm depth) soil temperature and water potential were measured at 30 locations (a monoculture of Elymus elymoides (Raf.) Swezey, four monocultures of Agropyron spp., 13 stands of Artemisia spp. and 12 stands of Juniperus spp. and Pinus spp. -Juniperus spp. woodlands ) in the Great Basin. Treatments evaluated included: (1) mow, (2) herbicide, and (3) prescribed burn for grasslands and shrublands. Woodland treatments included (1) cut and drop and (2) prescribed burn. At some sites, spring (Mar - Jun) soil temperatures spent 27% ± 1.14 SE of the season and 88 ± 3.28 SE degree days at the 0-5 °C temperature range when the soils were wet. The effects of site, season, year, and treatment were significant. A significant amount of thermal time at extreme temperatures would indicate the need for further examination of the thermal-time model at these temperatures. 0280 A method for characterizing western juniper tree densities in a sagebrush-steppe using NAIP orthoimagery and ArcView GIS Image Analyst Extension Marc Horney California Polytechnic State University, San Luis Obispo, San Luis Obispo, CA, USA This poster describes a method used to characterize tree density in a sagebrush-steppe biome, where western juniper (Juniperus occidentalis) is the only tree species of significance on the landscape. The method involves extracting geo-TIFF imagery from the National Agricultural Imagery Program (NAIP) orthomosaics and classifying them using the Image Analyst extension for Arcview 3.x GIS (Esri, Redlands, CA). By constructing an overlay of 1-hectare grid cells across a management area, the resulting classification can be used to characterize mature juniper tree densities across a heterogeneous landscape. This can afford a relatively inexpensive means of monitoring one particular aspect of an 81,000 hectare sage-grouse habitat management program in NE California, which can be conducted by resource-limited conservation organizations which possess at least some basic GIS capability. This method involves a number of trade-offs. The accuracy of juniper canopy determination is limited by the quality of the extracted images, and variations in shadows produced by different times of image acquisition and topography. Time required to classify images for very large areas can be substantial, especially for older processors 32-bit software systems. The resulting information can be a useful improvement over existing datasets, for guiding habitat management actions, however. 0281 Feral horse impacts on pronghorn use of watering holes on the Sheldon National Wildlife Refuge, Nevada. Amy Gooch1, Steven Petersen1, Gail Collins2, Tom Smith1, Brock McMillan1 1Brigham Young University, Provo, UT, USA, 2Sheldon-Hart Mountain National Wildlife Refuge Complex, Lakeview, OR, USA The purpose of this study is to investigate interference competition between pronghorn and feral horses at water in Nevada. Horses are considered large, aggressive animals that are socially dominant over native ungulate species. If horses are excluding pronghorn from water, this could have detrimental effects on pronghorn fitness, especially during the hottest months when water is limited. We placed motionsensitive cameras at 21 water sources across our study area to examine overlap of water use by pronghorn and horses and to determine if spatial or temporal partitioning of water is occurring. We also made direct observations of horses and pronghorn at high-use water sources, and recorded occurrences of negative pronghorn/horse interactions as well as changes in pronghorn behavior in the presence of horses. Preliminary results suggest that pronghorn spend more time on vigilance behavior in the presence of horses than in their absence, and that the majority of pronghorn/horse interactions at water result in pronghorn exclusion, sometimes due to horse aggression, supporting the hypothesis of interference competition for water in areas with high horse concentrations. 0283 A snapshot of Wyoming ranching operations: natural resource goals, context and management Emily Kachergis1, Justin Derner1, Jim Magagna2, Rachel Mealor3, Leslie Roche4, Lorien Jasny4, Mark Lubell4, Valerie Eviner4, Kenneth Tate0 1USDA-ARS, Cheyenne, WY, USA, 2Wyoming Stock Growers Association, Cheyenne, WY, USA, 3University of Wyoming Extension, Laramie, WY, USA, 4University of California-Davis, Davis, CA, USA Private ranches steward almost half of Wyoming's 62 million acres. Food production and conservation benefits depend on native ecosystems managed by these operations. What is a "typical" ranch in Wyoming, and how do ranches manage natural resources? In cooperation with the Wyoming Stock Growers Association (WSGA)-a predominant agricultural organization in the state-we asked WSGA producer members about their individual goals, ranching operation characteristics, and management practices via a mail survey. A total of 303 ranchers (46%) responded to the survey. Across this group of respondents, results indicate Wyoming ranches are very diverse in terms of land uses, ownership, vegetation types, and management. Several general themes emerged. Over 50% of reporting operations 1) consist of public or private leased land in addition to privately owned land, 2) graze cow-calf pairs in grassland and sagebrush ecosystems, and 3) include other activities (e.g. hunting, energy development) that affect land management. Livestock production and forage production were the top reported goals, with ecosystem characteristics that support these goals (e.g. soil health, water quality) coming in second. Over 50% of respondents reported they worked toward these goals via vegetation management and landscape enhancements, in addition to herd management and facilities. The diversity of operations suggests that efforts to improve food production and conservation in Wyoming should involve partnerships among landowners, public land managers, and other stakeholders at a landscape scale. Efforts that focus on livestock and forage production and supporting ecosystem functions are most likely to find synergies with ongoing management goals and strategies. 0287 Evaluating the Impact of Wyoming Big Sagebrush Fuel Loads on Bunchgrass Mortality Following a Fire Event April Hulet, Chad Boyd, Tony Svejcar Agricultural Research Service, Burns, OR, USA Fire temperatures can vary spatially due to fuel loading characteristics and burn conditions. The influence of fuel loads, particularly that of sagebrush, on bunchgrass mortality following a wildfire is largely unknown. In this study, we quantified pre-burn shrub fuel loads and its impact on bluebunch wheatgrass and Idaho fescue mortality. We used a randomized complete block design with varying amounts of shrub fuel loads per plot. Within each plot and for each bunchgrass species, two locations relative to sagebrush plants were selected: one within a sagebrush canopy and one in an interspace. Each bunchgrass was instrumented with four, type-K thermocouples immediately before the burn at the following locations: 2cm below the soil surface, at the growing point in the center of the plant, at the growing point within 2 cm of the edge of the plant, and 8-10 cm above the soil surface in the center of the plant. Preliminary results suggest that bunchgrass plants within sagebrush canopies were exposed to lethal temperatures (>50° C) longer than bunchgrass plants found in the interspace. Plots with greater sagebrush fuel loads, were more likely to create fire conditions severe enough to kill bunchgrass plants both within shrub canopies as well as interspace plants. As we increase our understanding regarding the influence of Wyoming big sagebrush on bunchgrass mortality, we can better prioritize fire rehabilitation efforts and identify communities where pre-emptive restoration practices can be implemented. 0288 Prediction of rangeland forage quality via near infrared spectroscopy in a mobile field laboratory. Stephen Prince1, Marco Bomfim1, Jay Angerer1, Doug Tolleson2 1Texas AgriLife Research, Center for Natural Resource Information Technology, Blackland Research and Extension Center, Temple, TX, USA, 2The University of Arizona, School of Natural Resources and the Environment, V Bar V Ranch, Camp Verde, AZ, USA The application of near infrared spectroscopy (NIRS) in natural resource management will be enhanced by the improved performance of calibrations in the field. Our objective was to evaluate the performance of crude protein (CP) and neutral detergent fiber (NDF) calibrations developed under mobile field laboratory conditions on a Perten 7200 diode array NIRS instrument. Thirty two samples from a variety of grass, shrub, succulent, and forb species were collected on a commercial cattle ranch in the Trans-Pecos area of West Texas in Spring 2012 and subjected to NIRS and reference chemistry procedures. The NIRS instrument, drying oven, and grinder were transported to the ranch and operated in a concrete-floored metal shop building. Spectra (950 to 1650 nm) were collected at the ranch on all samples after drying at 60 C overnight and grinding to 1mm. AOAC procedures were used to quantify CP and NDF at a Texas A&M University facility. Calibrations were developed in Grams software using partial least squares regression. Calibration performance was evaluated using cross validation. Mean and standard error (SE) for percent CP and NDF in the calibration set were 8.55 ± 1.08 and 55.77 ± 2.07 respectively. The CP calibration resulted in an R2 of 0.91 and SE of cross validation of 1.79. Similar calibration performance statistics for NDF were 0.91 and 3.47 respectively. Both forage quality constituents were predicted accurately enough to inform nutritional management of grazing animals. Larger calibration sets encompassing greater spatial and temporal variation should help improve NIRS performance in mobile laboratories. 0293 Rangland Management on the Seward Peninsul, Alaska Kacey Capuchino1, Joyce Pexton1, Greg Finstad2, Laurie Thorpe3 1University of Alaska, Fairbanks, Fairbanks Alaska, USA, 2Cal Poly San Luis Obispo, San Luis Obispo CA, USA, 3Alaskan BLM, Anchorage Alaska, USA Domestic reindeer are one of the primary range livestock used for red meat production in Alaska. However, there has been a lack of technical evaluation and monitoring the effect of reindeer grazing on their primary winter forage, lichen. A project was initiated to measure the impact of reindeer grazing on the Seward Peninsula to help estimatethe stocking density of ranges. Monitoring sites with exclosures were placed randomly to estimate lichen offtake and recovery rates. High resolution photography and physical estimation of percent-cover sampling of vegetation were used to establish baseline values. One of the challenges was to find a suitable design for exclosures to withstand the weather conditions such as wind and snow, as well as the permafrost and uneven terrain of the Alaskan tundra. Traditional post-inground construction isn't feasible because of the freezing and thawing of the tundra so "floating" exclosures were used that simply sit on top of the tundra in a circular design. The exclosures put up in the first year did have some engineering faults such as staying upright on steeper slopes. To correct this stakes and guide wires were attached uphill to keep the panels from collapsing inward. The exclosures and associated lichen and vegetation plots will be monitored for the next 30 years. 0304 California-Pacific Section Range and Natural Resources Youth Camp Curriculum - Modifications and Challenges Julie Finzel1, Marc Horney2, Cece Dahlstrom3, Theresa Becchetti4 1University of California Cooperative Extension, Bakersfield, CA, USA, 2Cal Poly San Luis Obispo, San Luis Obispo, CA, USA, 3Department of the Navy, San Diego, CA, USA, 4University of California Cooperative Extension, Modesto, CA, USA In the last five years, the Cal-Pac Section's Range Camp staff have made significant changes to the botany curriculum in order to facilitate a more practical understanding of plant ecology and to encourage students as they learn plant names for the first time. These included a booklet modeled after one developed by the International Mountain Section. This booklet provides prompts where students can take notes on the topics covered by speakers and can be used to study for the comprehensive exam that all students take at the end of camp. The camp's botany curriculum has also been shifted in the taxonomic characteristics it emphasizes, and the types of plant species included in their identification list. Plant ID scores have improved substantially in the last several years that these changes have been implemented. With these and other curriculum changes, the Cal-Pac Range Camp staff hope to improve the educational component of the camp, and teach some skills that may last longer with camp participants. 0308 VARIATIONS OF UNDERGROUND MORPHOLOGY OF FALCATA ALFALFA AFTER FIRST YEAR DORMANCY Lan Xu, Arvid Boe, Roger Gates, Patricia Johnson South Dakota State University, Brookings, SD, USA Plants persist in a community through regeneration initiated from dynamic seed and bud banks. Although seeds are important for dispersal, initial colonization, and maintenance of genetic diversity, under grazing seed production often is unreliable. Communities dominated by perennial species depend heavily on vegetative reproduction through bud banks. These dormant meristematic structures (i.e. rhizome buds, crown buds, and adventitious buds) represent a potential source of vegetative regeneration and regrowth. Yellow-flowered alfalfa (Medicago falcata) produces broad, deep-set crowns, a large number of rhizomes and adventitious buds and shoots on the roots. Our objectives are to evaluate variation in underground morphological traits contributing to vegetative reproduction and to identify potential parent materials for breeding grazing persistent alfalfa cultivars. Ten alfalfa entries, including one Medicago sativa control, three naturalized predominately yellow-flowered alfalfa populations, and six Medicago falcata populations were evaluated. One hundred uniform seeds were selected from each entry. Seeds were scarified and inoculated with rhizobium before planting in plastic cone containers. After seedling completed first year dormancy under an ambient day/night photoperiod cycle in a greenhouse, ten plants from each population were randomly selected and excavated to examine variation among underground morphological traits: crown diameter, rhizome, branch root, adventitious root number, angle and number of shoots from crown. Additionally, 25 plants from each population were randomly selected to assess the capacity of root segments to produce adventitious shoots. 0309 Groundwater Movement and Loss through Anthropogenic Ponds in Restored Meadow Streams Adam McMahon, Sherman Swanson University of Nevada, Reno, Reno, NV, USA Stream degradation in the Sierra Nevada has led to restoration using pond-and-plug techniques. This technique uses earth dams or plugs to fill eroded gullies, which then fill with water, creating a series of ponds. Stream flows divert into an historic or designed channel that is connected to the historic flood plain. Raised groundwater elevations, increased flooding frequency and energy dissipation return meadows to natural function and wet-meadow vegetation rebounds. Little research has addressed the hydrology of the created ponds, which present a large surface area of open water to evaporation. This may be a large "sink" or water loss. Three hydrologic models have been proposed for groundwater movement in meadows, "sponge, valve, and drain". Meadows can: soak and slow the movement of groundwater, supply groundwater through springs, or drain groundwater through deep discharge. Objectives of this research are to: 1. Compare water levels in ponds and streams to describe aquifer recharge; 2. Evaluate pond water levels through time and space to establish which hydrologic model each meadow may resemble; and 3. Examine whether pond evaporation is reducing stream base flows in the late season. Water Level Data Loggers and surveying technique will record: 1. Pond water elevation; and 2. Pond water surface area. Stream discharge will be measured entering and leaving restored areas using stage-discharge relationships and water level loggers. Evaporation will be calculated from regional metrological stations. Collected data will be analyzed over time and distance. Surface water elevations between ponds and streams will be compared to observe interaction. 0315 Sage-grouse habitat models for the state of utah. Chris Balzotti1 ,2, Philip Dennison1, Steven Petersen2, Randy Larsen2, Richard Forester1, Mitchell Power1 1University of Utah, SLC UTAH, USA, 2BYU, provo utah, USA Concern over greater sage-grouse management has increased over recent years and Utah land managers are in the process of improving their sage-grouse protection plans. Some of the challenges to sage-grouse management in Utah are the extensive areas of potential habitat, the cost of assessment and the diverse land ownership (private, BLM, DWR, Forest Service, Native American, etc.). To assist with this process, we created statewide predictive models using multiple methods to delineate potential sage-grouse habitat. Non-parametric multiplicative regression was utilized to create sagebrush presence models. Total vegetation cover models were created using generalized additive models. These were then combined with topographic and climate variables in Maxent to delineate potential sage-grouse habitat. Finally, multiple global climate and emission scenarios were used to identify sagebrush areas within the state of Utah that may be prone to change for years 2020, 2050, and 2080. All models, with the exception of climate models, were assessed using both model strength (during creation) and validation (as defined by overall accuracy compared to field collected data). The statewide sagebrush cover model (sagebrush greater than 5 percent) had a logB of 6.4 and an overall accuracy of 63%. The total vegetation model had a R2 of 0.596 and an overall accuracy of 70% (within 10% of ground data). The Sage-grouse nest model had an AUC of 0.93 and an overall accuracy of 91%. 0316 Wild Horse and Burro Utilization and Population Estimates in the Cibola Trigo Herd Management Area John Hall Bureau of Land Management, Yuma, az, USA The Cibola-Trigo Herd Management Area, managed by the Bureau of Land Management (BLM), is overpopulated by wild burros, with approximately 215 wild burros exceeding the appropriate management level. In order to manage the population at a thriving natural ecological balance, annual utilization studies and a population estimate every three years are conducted in collaboration with Arizona Game and Fish, the Fish and Wildlife Service, and Yuma Proving Grounds. The current data collection method is aimed at determining if a relationship exists between utilization rate and population size. Preliminary results show there is a slight correlation between utilization and population size (R 2= 0.0313). Continuing data collection may show a stronger correlation between utilization rate and population size. 0318 The influence of fire severity on ecological succession patterns within an aspen-conifer post-fire community Steven Petersen1, Standley Kitchen0 ,2 1Brigham Young University, Provo, Utah, USA, 2Rocky Mountain Research Station, Shrub Sciences Laboratory, Provo, Utah, USA Post-fire succession within mixed aspen-confier woodlands of western North America is poorly understood. Research is needed that investigates the influence of fire severity on both short-term and long-term plant community establishment and succession. Between July 20 to October 16, 2010, the Twitchell Canyon fire burned across the Tushar mountains in southwestern Utah consuming approximately 18,160 hectares. The fire burned with variable severity through single or mixed stands of quaking aspen (Populus tremuloides), ponderosa pine (Pinus ponderosa), Engelman spruce (Picea engelmannii, Douglas fir (Pseudotsuga mensezii) and white fir (Abies concolor). Fire severity classes (low, moderate, high, none) were determined using Landsat satellite imagery. Tree age was measured by counting the rings of cores collected from a subset of trees within each plot. Tree strucutre was calculated using DBH and distance based measurements. Herbaceous cover and frequency were estimated using the point intercept and nested frequency methods. Aspen regeneration was determined by counting the number of shoots within subplots. Results indicate that across all vegetaion types, low, moderate, and high severity caused 35, 69, and 100% tree mortality. Aspen shoots were found on 81% of plots across all severity classes. Understory species were dominated in cover by root sprouters (both herbacous and shrubs) and disturbance adapted herbacous species. Using these data, managers can predict succession patterns in aspen and mixed-aspen communities after fire. 0319 Improving rangeland seeding success in post-fire water repellent soil using surfactant seed coating technology Matthew Madsen1, Daniel Zvirzdin2, Kaitlynn Fernelius2, Bruce Roundy2 1USDA - Agricultural Research Service, Burns, OR, USA, 2Brigham Young University, Provo, UT, USA Severe disturbance from catastrophic wildfires often requires that native plant materials be reintroduced through reseeding, but the success rate of these restoration efforts in arid environments is notoriously low. Post-fire soil water repellency can limit reseeding success by decreasing soil moisture for seed germination and establishment. Surfactant seed coating (SSC) technology may help to overcome this limitation. Laboratory testing has shown that by coating seeds with soil surfactant, water repellency within the seed's microsite can be ameliorated, which increases root-zone water reserves and enhances seedling emergence and plant survival. The objective of this research was to evaluate the influence of SSC technology on plant establishment within recently burned pinyon-juniper (Pinus-Juniperus) woodlands. The study design consisted of a randomized complete-block design with two treatments (seeds left uncoated or coated with SSC) applied to two bunchgrass species. We implemented the treatments in 2010 and 2011 at two separate wildfire locations. At the 2010 site, plant establishment from SSC was between 2.0-4.2 fold higher than the uncoated seed two years after planting. Plantings in 2011 had 7.5-fold more seedlings emerge in the SSC treatment, for one of the two species sown the first year after treatment; however, due to severe drought conditions the majority of emerged seedlings desiccated during the first year, resulting in poor plant establishment for all treatments (i.e. less than 1-plant·m-2). These preliminary results demonstrate the ability of SSC technology to improve reseeding success, but additional research is needed to further improve the efficacy of SSC technology on drought years. 0321 Carbon Stock in Chihuahua grasslands under different range conditions Pedro Jurado-Guerra1, Martin Juárez-Morales2, Carlos Ortega-Ochoa2, Alicia Melgoza-Castillo2 1Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Chihuahua, Mexico, 2Universidad Autónoma de Chihuahua, Chihuahua, Mexico Range condition as a result of rangeland management and climate might have an effect on soil carbon stocks. The objective was to determine soil carbon stocks at shortgrass prairies with different range condition in Chihuahua, Mexico. Four sites with different plant cover and productivity were selected in central and southern Chihuahua. In each site, three field plots with three 50 m transects each were established. Plant composition was estimated using the point intercept method. Range condition was determined in each site using the climax method. Soil samples were taken at each transect at two microsites (bare soil and underneath B. gracilis) and two soil depths (0-15 and 15-30 cm). A composite sample was used for chemical analysis. Soil organic carbon (SOC) was estimated through an elemental analyzer equipment. Soil carbon stocks were estimated using SOC and soil bulk density. Most abundant grasses were Bouteloua gracilis and Aristida spp. Based on plant composition, two sites were found at poor range condition and two at good range condition. Data was analyzed with lineal models to determine condition, microsite and soil depth effects. Results showed both condition and microsite significant effects (P≤0.001). Total soil carbon was 23.30 ton C ha-1 and 26.72 t C ha-1 for bare soil and underneath B. gracilis, respectively. Soil carbon stock appeared to be greater under good range condition and underneath grass plants. Soil depth did not influence soil carbon stocks. 0322 Use of Biosol Forte as a seed coating to improve stand establishment of native bunchgrass species Matthew Madsen, Kristen Munday USDA - Agricultural Research Service, Burns, OR, USA Applying fertilizers at the time of planting may improve native plant establishment by increasing the ability of the seedlings to cope with environmental stresses. However, traditional fertilizer applications are often economically infeasible and may be detrimental by encouraging weed invasion. Seed coating technology allows for the efficient application of fertilizers within the microsite of the seeded species. The objective of this study was to evaluate the efficacy of the slow-release fertilizer Biosol Forte® for use as a seed coating treatment to improve seedling emergence and plant establishment. Biosol coated seeds and nontreated seed were compared, using Pseudoroegneria spicata and Leymus cinereus as the model species. Seeds were coated with Biosol in a rotary coater at 60% weight of product per weight of seed. The study design consisted of factorially arranging the two species and two treatments in a randomized completeblock design, within a droughty-loam ecological site, in eastern Oregon, USA. Plant density was counted in the spring of the first year (May 2012). At the end of the growing season (August 2012) plant density was recounted and above-ground biomass was harvested. Biosol coated seed increased the number of established L. cinereus plants by 130% and improved biomass production by 156%. Established plant density and biomass production of P. spicata was 49% and 53% higher in the Biosol coating treatment. These preliminary results indicate that a Biosol seed coating can be effective in improving stand establishment of native bunchgrass species. Additional research is needed to determine long-term response of the treatment. 0324 Post-Fire Soil Water Repellency: Persistence within Piñon-Juniper Woodlands Daniel Zvirzdin1, Matthew Madsen2, Bruce Roundy0 1Brigham Young University, Provo, UT, USA, 2USDA - Agricultural Research Service, Burn, OR, USA Erosion and weed dominance often limit the recovery of burned piñon-juniper woodlands. Soil water repellency (SWR) is one factor that may contribute to this by increasing overland flow and impeding seedling establishment. Previous research has shown that SWR is widespread within piñon-juniper woodlands; however, the persistence of SWR within these systems is unknown. In this study, SWR extent, severity, and thickness were sampled for three years within 41 1000 m2 subplots nested in three major wildfires that burned in the Great Basin in 2009. These data were used in concert to determine the effects of climate, topography, soil attributes, burn severity, and piñon-juniper height, width, canopy cover, and age on SWR persistence. Across the study, SWR was found at 37% of the points sampled in the first year, 22% in the second, and 18% in the third; over 80% of the total decline in SWR extent occurred in the first year. This decline followed no clear trends relative to ecological site characteristics, especially when viewed at the subplot level. However, the severity of SWR the first year following fire showed some relative strength to SWR persistence: sites with the highest SWR severity also had the lowest declines in SWR extent over time. As invasive weeds often colonize areas where SWR has dissipated, these results support the conclusion that if weed dominance is to be averted in areas with moderate levels of SWR, desirable species need to be quickly established the first year after fire. 0325 Are early summer wildfires an opportunity to revegetate medusahead-invaded rangelands? Dustin Johnson1, Aleta Nafus1, Kirk Davies2 1Oregon State University, Burns, OR, USA, 2USDA-Agricultural Research Service, Burns, OR, USA Successful revegetation of medusdahead-invaded plant communities can be prohibitively expensive, because it often requires iterative applications of integrated control and revegetation treatments. Prescribed burning has been used to control medusahead and prepare seedbeds for revegetation, but burning has been constrained by liability concerns and produced widely varying results. Capitalizing on naturally occurring wildfires could reduce revegetation costs and alleviate liability concerns. Thus, our objective was to determine if early summer wildfires and fall drill seeding could be used as a treatment combination to decrease medusahead and increase perennial and native vegetation. Treatments were evaluated pre-treatment and for three years post-fire at six sites and included: 1) an early summer wildfire combined with a seeding treatment (burn and seed) and 2) a non-treated (no burn, no seed) control. Perennial grass density was 4.6- to 10.0-fold greater in the burn and seed treatment compared to the control in the first three years post-treatment (P < 0.05). Exotic annual grass density and cover in the third year post-treatment were lower in burn and seed treatment than in the control (P < 0.05). However, exotic annual grass density was still > 130 individuals·m-2 in the burn and seed treatment. The density of exotic annual grass is of concern because over time medusahead may displace perennial grasses and annual forbs that increased with the burn and seed treatment. However, it appears that early summer wildfires may provide an opportunity to reduce the cost of integrated programs to revegetate medusahead-invaded plant communities. 0327 Nitrogen fertilization and ration supplementation reduces annuals in smooth bromegrass pastures John Guretzky, Walter Schacht, Laura Snell, Jonathan Soper, Stephanie Pruitt, Andrea Watson, Terry Klopfenstein University of Nebraska, Lincoln, NE, USA Supplementation of corn (Zea mays L.) dried distillers grains plus solubles (DDGS) reduces forage demand and improves N use efficiency of beef cattle. Our objective was to evaluate how supplementation of DDGS on unfertilized pasture (SUPP) affects botanical composition relative to unsupplemented beef cattle on N fertilized (FERT) and unfertilized control (CONT) smooth bromegrass (Bromus inermis Leyss.) pasture. From 2010 to 2011, we measured relative basal cover, aerial cover, and frequency of occurrence of plant species and functional groups, as well as forage yields, within an ongoing experiment near Mead, NE, where N input has averaged 6.5, 55.4, and 96.5 kg ha -1 yr-1 within CONT, SUPP, and FERT, respectively, since 2005. We found relative basal and foliar cover of plant species and functional groups depended on treatment and year interactions. From 2010 to 2011, relative basal cover of smooth bromegrass decreased by 23.6, 11.6, and 3.9 percentage points while relative basal cover of annuals increased by 18.3, 8.2, and 0.8 percentage points in CONT, SUPP, and FERT, respectively. Measurements of frequency of occurrence showed annuals were most in CONT, intermediate in SUPP, and least in FERT in both years. Forage yield of smooth bromegrass averaged 6.063, 6.496, and 10.437 Mg ha-1 yr-1 in CONT, SUPP, and FERT, respectively. Supplementation of DDGS on unfertilized pastures improves beef cattle weight gains, production, and N use efficiency relative to unsupplemented cattle on fertilized and unfertilized pastures while providing an intermediate level of annuals.