Title: A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes
Journal name: Molecular Breeding
Authors: Andrea Mazzucato, Francesco Cellini, Mondher Bouzayen, Mohamed Zouine, Isabelle Mila, Silvia Minoia, Angelo Petrozza, Maurizio E.
Picarella, Fabrizio Ruiu, Filomena Carriero
Corresponding author: Dr. Filomena Carriero, Alsia-Centro Ricerche Metapontum Agrobios, S.S. Jonica 106 Km 448.2, 75012 Metaponto (MT),
Italy email: filomena.carriero@alsia.it
Table S2 Expression of parthenocarpy and other fruit related traits in natural and transgenic sources of parthenocarpy compared with the respective, near isogenic
wild-type (WT). The table includes only systems for which suitable information is available.
Type of
parthenocarpy
Genetic/
natural
Genetic
background
pat
Chico III
OP
0
59 to 95b
pat-2
Several
OP
1 to 33
20 to 87c
pat-3
/pat-4
IAA9
-d
OP
-
44
=
-
>
-
MicroTom
Red
Setter
E (+10)
3
40 to 70
=
-
-
E (+15)
OP
7
0 to 4
39
68 to 93
= or >
>
UC82b
E
-
100
<
More
elong
ated
More
elong
ated
Misshapen
=
- Thicker pericarp, late
ripening
= Anther and ovule defects
-
-
IAA9
Transgenic
(loss of
function)
Della
Aucsia
Flower
treatmenta
Parthenocarpy (%)a
Mutation/
Gene
involved
WT
mutant
Characteristics of seedless
Other reproductive traits
fruits compared to WT
distinctive of the mutant
Size
Shape °Brix Firmness
<
=
>
< Anther and ovule defects,
early flowering and
ripening
= or <
=
>
< Late flowering
References
Mapelli et al. 1978
Mazzucato et al. 1998
Philouze et al. 1988
Casas Diaz et al. 1987
Philouze 1984
Casas Diaz et al. 1987
Gourguet et al. 2005
Saito et al. 2011
This work
Martì et al. 2007
Breeding
E
0
44-100
<
Molesini et al. 2009
lines
IAA9
Micro
E
0
48 to 82
=
= Subtle ovule defects
Wang et al. 2005
Tom
Wang et al. 2009
Ailsa
Craig
Transgenic
IAAM
Breeding
E
0
63 to 100
=
= = or >
=
Ficcadenti et al. 1999
(gain of
lines
function)
rolB
Breeding
OP
Variable
>
=
>
- Early ripening
Carmi et al. 2003
lines
a
parthenocarpy estimated as the percentage of emasculated not pollinated flowers developing (E, in parenthesis the number of d after emasculation the fruit set was
observed) or as percentage of seedless fruits at maturity after open pollination (OP) of flowers
b
depending on temperature conditions at flowering
c
depending on the genetic background
d
not determined or not reported