ANIMAL ETHICS APPLICATION FOR APPROVAL TO
ESTABLISH AND MAINTAIN A BREEDING LINE
The purpose of this form is to allow the AEC to be informed of the potential Welfare Implications of the
animals you wish to introduce/breed. It also informs the AEC of the proportion of animals bred which are
ultimately useful for your purposes (i.e. the wastage rate).
If the animals being bred are genetically modified the attachment 'Phenotype Passport - Phenotype report
for genetically modified (GM) animals' must be completed and submitted with this application
Instructions
 After completing this form please convert to a pdf and add any required pdf attachments in the
relevant sections;
 For the signature section, we suggest printing out the relevant page(s) and after scanning
signatures, replace these pages in the pdf version of the document;
 Please note that printed copies will NOT be accepted, nor will Microsoft Word versions of the
document; it must be a pdf version;
 To submit, email the final pdf to secretary.aec@sahmri.com.
Bioresources is a PC2 and SOPF facility and all animals housed here will need to comply with this
health status. Please contact Bioresources for further information. We can offer rederivation
services to restore your colony to a specified and opportunistic pathogen free (SOPF) health
status.
Common Name of Line
Exact Nomenclature1
Background Strain2
Nomenclature of genetic alterations (if any) 1
Number of Backcross Generations3
Contact
e.g. Facility Manager, Scientist
Name (include title)
Applicant's Institution and
Department
Contact details
(including After Hours)
Email
Phone
Mobile
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Details of modification45
References and Websites5
Origin of strain (location and researchers)6
Genotyping Method7
Please attach a protocol; send control DNA if possible.
Husbandry Recommendations
1. What diet and bedding have been used?8
2. What housing system has been used and what is most appropriate?9
3. What environmental enrichment has been used?10
4. Current breeding strategy (e.g. heterozygote x heterozygote)?
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5. Breeding information
Average litter size:
Pre-weaning mortality:
Post-weaning mortality:
Fecundity limit (age):
6. What containment category is appropriate for the animals (PC1-4)?
7. Detail the current environmental parameters for the animal (e.g. temperate, humidity, light cycle)
8. Breeding recommendations11
9. Other
Phenotype
1. Current health status of the colony12
2. General appearance and coat colour
3. Any physical abnormalities?
Yes
No
If yes, please detail and indicate if any remedial action is necessary
4. Are there any behavioural traits e.g, aggression, termor, abnormal movement?
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Yes
No
If yes, please detail and indicate if any remedial action is necessary
5. Has a welfare assessment been carried out on the line?13
Yes
No
If yes, detail over what period assessment was carried out and findings.
6. Have any specific phenotype test been carried out? 14
Yes
No
If yes, detail which tests and findings. Include published references if relevant?
7. What is the immune status of the line e.g. normal, deficient?
8. If a GM line, describe any issues associated with homozygosity versus heterozygosity for the mutation
e.g. lethality.
9. Growth rate
10. Other phenotype observations
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Date Passport Last Updated
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Attachment: Mouse Passport
Attach as pdf pages or if text by copy-paste into field below
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Attachment: Other
Attach as pdf pages or if text by copy-paste into field below
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Passport Guidance Notes
The mouse passport is intended to provide husbandry and welfare information for establishments receiving
genetically altered mice. The passport does not replace any requirements for Home Office permission for
Movement, Defra import licences or Health Certification.
The passport is provided for information and guidance and the originating establishment accepts no
liability arising from the use of information provided in this document.
1. Details of correct strain nomenclature for rodents can be found at
http://www.informatics.jax.org/mgihome/nomen/#rag.
2. Details of the original genetic background should be given.
3. Details of any subsequent breeding should be given.
4. Please give details of the modification e.g. transgene (and, where known, copy number if
generated by pronuclear microinjection or random integration) or targeting construct and ES cell
details; or TALEN or Crispr-Cas mutation if appropriate. If mice are genetic mutants, give details
of the nature of the mutation and whether original mice were spontaneous or induced mutants).
5. Please give details of any publications or websites that deal specifically with the strain. If
substantial information is available please give the most comprehensive or recent reference
6. This should refer to the originator of the GA mice and the contact details in order to obtain
permission to use the mice. Details should include where and when the mouse was generated.
7. Please give details of the genotyping procedure. Is screening by Southern Blotting, PCR, ELISA,
physical appearance or phenotypic expression? Please attach a protocol for the screening method.
8. Are any dietary supplements required i.e.: Lectade rehydration therapy? Please provide a reason
as to why this may be necessary.
9. What is the most appropriate housing system? For example, conventional housing, I.V.C, isolators,
containment or quarantine.
10. This would include nesting material and other enrichment devices found to be useful or
inappropriate.
11. Breeding recommendations. It is important to state which animals (male, female, all) carry the
relevant transgene for breeding and welfare purposes. Details of system used to breed mice and
any comments relating to breeding management (e.g. age for first mating, pairs or harems, inbred
or outbred, etc.
12. Any known microbiological contamination? Include any information on what microorganisms were
included in the screen, the length of time between each screen and the method used (e.g.
sentinels or littermates).
13. Welfare assessments should be carried out for the period that adults are normally maintained for
that particular line, and the limit to this should be noted.
14. Specific phenotypic tests include post mortem data, histology and any other analyses performed
on the mice. Please give references if applicable.
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