Comparing high and low input systems to manage soil organic carbon Frances Hoyle, Natalie Hogg, Justin Laycock, Liam Ryan (Department of Agriculture and Food WA) and North-Stirling Natural Resources Group staff Key messages Increasing fertiliser rates did not result in measurable yield responses and at high rates decreased grain protein in canola. There were no agronomic benefits measured in response to the application of biochar. No measureable change in soil organic carbon or other soil properties was determined from imposed treatments. Biochar application may be decreasing mineralisation (breakdown) of background SOC in surface soil. No agronomic response = no economic benefit observed as a result of imposed treatments. Aim To identify what, if any, effect altering inputs to increase plant biomass would have on soil carbon, and whether amending soils with biochar would increase soil carbon storage or provide agronomic benefits. Background Growers are constantly assessing the long term profitability and sustainability of their farming systems. Often growers look to target an optimum gross margin rather than highest yield. This demonstration trial was established in 2013 and carried on into 2014 to determine whether measureable changes in (SOC) and productivity could be associated with higher amounts of organic inputs. The question being posed at this site was whether changes in net primary productivity (NPP) or the application of biochar would influence short term soil organic carbon levels by manipulating the organic inputs entering soil. In this instance three rates of fertiliser were used, and the biochar assessed in comparison to a control that had been ripped and harrowed. Supporting your success Trial details Property Plot size and replications Soil type Paddock rotation Treatments Clear Valley, Gnowangerup 100m x 18.2m x 3 replicates Shallow duplex - gravelly sand over clay to shallow sand over clay, sloping site 2012: Canola 2011: Pasture 2010: Wheat 2009: Pasture 1. Farmer practice (55 kg/ha NPK crop + 15 kg/ha sulphate of ammonia) 2. Low Fertiliser (35 kg/ha NPK crop + 10 kg/ha sulphate of ammonia) 3. High Fertiliser (79 kg/ha NPK crop + 21 kg/ha sulphate of ammonia) 4. Rip and harrow (Plus low fertiliser) 5. Biochar 1.5 t/ha (Plus rip and harrow, low fertiliser) Activity 2013 2014 Crop type Canola (Hyola 559) Wheat (Mace) Sowing date 3 May 26 May Seeding rate 2.6 kg/ha 60 kg/ha Growing season rainfall 209 mm 314 mm 550 g of Atrazine 500 g Propyzamide 1.2L glyphosate 100ml Alfa-cypermethrin 100ml Diametholote Herbicide pre-emergent 500 g of Propyzamide 200 ml Bifenthrin 300 ml Chlorpyifos 1 L gromoxone 300 gms diuron 118 gms sakura Herbicide post-emergent Atrazine 1.1 kg Select 500 ml 440ml LVE MCPA 570 Herbicide knockdown 8 June 19 L/ha Black Urea 26 June 21L/ha Black Urea Fertiliser 30L Hi Tech Black Urea (post-em) 7 August 30L Hi Tech BluS 1L Hi Potash 2L Poly PK 500ml fulvic 200ml fulva wet 1 September 25L Black Urea 500ml fulvic 2L Poly PK 300ml boron 1L Hi Pot 1L Hi 3 (Traces blend) 2L Hi Calcium 200ml Fulva wet *fertiliser is from Australian Mineral Fertilisers. Results 2013 Soil Baseline soil sampling was conducted in March 2013 (Table 1). There was low to moderate water repellence measured in the surface soil (0-10 cm) at this site using the molarity of ethanol droplet test, but none measured for soils below 10 cm. Soil pH increased with depth and was considered above the critical target of pH 4.8 in the subsoil but below the critical target of pH 5.5 in the surface (Table 1). Soil was marginal for nitrogen and low in potassium (Table 1). Water holding capacity of the soil was 21% (average all depths). Table 1 Baseline soil test results from the trial site prior to the implementation of treatments in March 2013. Depth (cm) BD (g/cm3) pH (CaCl2) Total SOC (%) SOC stock (t C/ha) 0-10 1.48 4.9 1.1 16.8 13 7 20 31 28 26 10-20 1.65 4.8 0.5 7.7 12 4 5 23 18 8 20-30 1.61 5.1 0.3 2.4 12 3 4 21 22 10 C/N NH4 ratio (mg/kg) P K NO3 S (Colwell, (Colwell, (mg/kg) (mg/kg) mg/kg) mg/kg) Grain yield The site averaged 0.86 t/ha canola and 49.8% oil but there were no treatment differences in grain yield or oil measured. Grain moisture averaged 4.6%. Grain protein was significantly (p < 0.05) higher on the farmer practice and low fertiliser treatments (average 18.5%) and lowest on the biochar treatment (average 17.5%). However this difference was not large enough to influence the total amount of nitrogen (N) taken up by the plant which averaged 26 kg/ha N across all treatments. Site influences were also evident in higher amounts of admixture in plots at the top end of the trial area where there was a greater depth to clay in the subsoil. However this was not evident in any one treatment. 2014 Soil The carbon to nitrogen ratio (16:1) of the soil was typical of the range found in WA soils. Moderate water repellence was evident for surface soil in soils that had been ripped. Other treatments showed low risk of water repellence. Soil pH was nominally higher (pH 5.4 decreasing to 5.0 below 10cm) than when measured in 2013 but showed no treatment responses. Soil ammonium levels averaged 3 mg/kg and though there was slightly higher levels for the topsoil (0-10cm) in low input and biochar treatments this change was <1.5 mg/kg and was not considered further. Nitrate concentration was also low at this site (<10 mg/kg). Sulphur was highest in the surface (15 mg/kg) decreasing to 5 mg/kg below 10cm. Soluble and small particulate organic carbon (DOC) was approximately 20% higher in ripped treatments than in un-ripped treatments with either normal or high fertiliser rates, suggesting a release of ‘new’ labile carbon previously protected by the soil structure (data not presented). The concentration of DOC - which is a suitable food substrate for soil organisms - is highly correlated to both the microbial biomass (MBC) and greater biological soil nitrogen supply (potentially mineralisable nitrogen; PMN; Table 2). Table 2 Soil testing results from the trial site after the implementation of treatments in March 2014. Soil depth (cm) 0-10 10-20 20-30 LSD BD (g/cm3) Clay (%) 1.7 1.8 1.8 p<0.05 4 4 7 2 SOC stock (t C/ha) 16 6 5 1 SOC CEC DOC MBC PMN P K (%) (meq/100g) (mg/kg) (mg/kg) (mg/kg) (mg/kg) (mg/kg) 0.99 0.35 0.27 0.06 3.3 1.4 1.5 0.3 106 45 42 10 141 35 22 12 11 1 <1 1 28 21 16 6 40 21 18 4 Soil organic carbon stocks (t C/ha) No significant changes in soil organic carbon stocks were measured at this site. Soil organic carbon stocks were variable across the site and while a slightly higher carbon stock is associated with the biochar treatment this is not considered different to the rip and harrow treatment (Figure 1). High variability in carbon together with a large carbon background (25 t C/ha) make it difficult to measure the small change in carbon associated with the biochar. At an application rate of 1.5 t/ha biochar and assuming 40% carbon content, this treatment only added 0.6 tonnes carbon per hectare - so there is insufficient confidence to suggest the ‘difference’ indicated below is real. 40 30 20 10 0 High fert Control (100kg/ha) (70 kg/ha) Low fert Biochar (40 kg/ha) (rip+harrow) Rip and harrow Figure 1 Soil organic carbon (t C/ha, 0-30cm) in 2014 under treatments imposed in 2013. Bar is the standard error of the mean. Grain yield The site averaged 2.45 t/ha of wheat with no treatment differences measured (Figure 2) and no signficant change in grain protein yield (nitrogen uptake in grain; Figure 2). There were no differences in grain quality receival standards (hectolitre weight = 77 kg/hl; small grain screenings = 6%; protein at 11% moisture = 8.1%) . 3.5 Grain yield (t/ha) 3.0 2.5 2.0 1.5 1.0 29 32 35 31 39 High fert (100kg/ha) Control (70 kg/ha) Low fert (40 kg/ha) Biochar (rip+harrow) Rip and harrow 0.5 0.0 Figure 2 Grain yield (bars) and nitrogen uptake (numbers in bars, kg N/ha) of wheat in 2014 under treatments imposed in 2013. Bar is the standard error of the mean. Economic analysis While no extensive analysis has been done on the economics of these applied treatments, the application of biochar at 1.5 t/ha did not result in any measureable agronomic benefit and thus to date has been an expense with no associated return. Ripping and harrowing in this instance did not return any further gains in terms of yield or quality at this site in 2013 compared to the control and again did not appear to address a significant constraint at this site in this year. Higher fertiliser rates did not affect grain yield or quality. Thus in this instance the ‘best’ treatment (i.e. ‘most profitable’) would have been the low fertiliser input treatment with lower associated input costs and achieving the same grain yield and quality. Comments The site is situated on a slope and has a gradual change in depth to clay moving down slope. In 2013 and 2014, despite low soil nitrogen levels there was likely sufficient nitrogen applied in basal application of black urea to supply crop requirements and it is not surprising that the small change in nutrient addition - equivalent to 3 kg/ha nitrogen, 2 kg/ha phosphorous, 1 kg/ha potassium and 1.5 kg/ha sulphur between applied fertiliser treatments, combined with dry periods during crop growth was not evident in terms of measureable plant responses. Changes in soil organic carbon take time and are difficult to measure due to large spatial (and temporal) variability. The application of organic or carbon based inputs must be put in context to a large background stock and sampling induced errors. A good question to ask in the case of considering why you might try a new product is whether it provides a specific function or role that is missing from your soil, or whether it is applied at a rate sufficient to actually make a measureable change. A great approach is to ‘strip’ test treatments in a paddock making sure that you also represent your ‘normal’ farming practice – measure a change in the attribute of interest - and go from there. Abbreviation Meaning kg/ha t/ha L/ha kg/hl mg/kg g/cm3 mm Kg C/ha N/ha LSD CEC N C NH4 NO3 P K S SOC CaCl2 BD MCPA LVE MBC PMN C/N ratio N/ha DOC Kilogram per hectare Tonne per hectare Litre per hectare Kilogram per hectolitre Milligram per kilogram Gram per cubic centimetre Millimetre Kilogram Carbon per hectare Nitrogen per hectare Least significant difference Cation exchange capacity Nitrogen Carbon Ammonium Nitrate Phosphorus Potassium Sulphur Soil organic carbon Calcium chloride Bulk density MCPA herbicide (present as the ethylhexyl ester) low volatile ester Microbial biomass-carbon Potentially mineralisable nitrogen Carbon nitrogen ratio Nitrogen per hectare Soluble and small particulate organic carbon in solution Acknowledgements Thanks to the Facey group and their growers for continued interest and support for this project and management of the trial site. A special thanks to Kevin Wise for access to the trial site and assistance for the duration of the trial. Also thanks to the North-Stirlings Pallinup Natural Resource Group staff for help in trial site sampling and Richard Bowles (UWA) for analyses. This project is led by the Department of Agriculture & Food WA in collaboration with the North-Stirlings Natural Resources Group and is supported by funding from the Australian Government Department of Agriculture - Action on the Ground program and the GRDC. Contact: Frances Hoyle frances.hoyle@agric.wa.gov.au