CHAPTER-III
Method of Study
For selection of Cholesterol Affected individual person Advertise
published in news paper that person who have cholesterol problem if they want free
treatment on their disease without taking drugs, only cholesterol control by exercise
and food supplement can call on this mobile No. 7709003653 for taking part into
cholesterol controlling programme.
150 person Men and women examined and
declared 100 of them were medically fit for this study and they were selected by lot
method and they divided randomly in to two groups, Men and women group separated
as one control and one experimental group. The selected Hundred subjects were
randomly divided in to four groups of twenty five each, out of which group I (N-25)
Underwent running activity and Food Package and group II (N-25) remained as
control. Pre test were conducted for all two groups on Low Density Lipoproteins,
Triglyceride, Total Cholesterol and High Density Lipoproteins. The experimental
group participated in their respective Exercise programme including Walking and
other Aerobic for a period of Twelve month. Post test were conducted on the above
mentioned dependent variables after Twelve month of the training period. The
training programme scheduled at morning 6.00 a.m. to 7.00 a.m. and evening 6.00
p.m. to 7.00 p.m. The advises were given walking exercise for 30 minutes at morning
and evening. Every exercise session should include a warm-up for 5 to 10 min. must
mean we have to stress your hand and legs relax, neck by moving roundly then start
exercise, at the time of exercise pulse rate must be increase 45% to 80% then
functional capacity and then stay constant for 60 min. at the time of stopping exercise,
stop slowly for 5-10 min. cool down phase.
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During this time they were closely observed, their dietary pattern assessed by
a questionnaire. They were given knowledge about the risk factors of coronary artery
disease and that disease could be reversed by changing diet. they were advised to
follow a modified diet schedule based on Diet Manual. The modified diet included
more vegetables and fruits, with moderate amount of rice and pulses. They were
advised to keep away from the possible risk factors like alcohol, smoking. Cooking
Food Package:Before exercise: 1 Cup green tea or skimmed milk,/ Fenugreek seed + wheat Bran +
semolina make sweetmeat ball of normal size
Breakfast: 50gm of cereal + 25 gm of leg tunes + 250gm of Vegetables (like salad or
curries). Or 200ml Any Juice – Orange, pineapple, Sweet lime, carrot, luffa
Lunch : 50gm. of cereals + 50 gm. of leafy vegetables + 1 00gm. of other vegetables.
4 O ‘clock : 1 glass of any type of juice + any types of fruits 1oo gm.
Dinner: 2 Chappathees with vegetable cury or equivalent. Or Khichari like jawar,
maize, pigeon pea. Oil – Ground nut, karadi, sesame
(Patients were supplied with a dietary guide describing various choices of food).
If patients like to take non vegetarian food, they were allowed to
take 100 g of fish thrice in a week, 100 g of skin removed chicken twice in a month.
They were also allowed to take egg white, but not egg yolks.
Sources of data
To execute these investigation twenty five males and twenty five females of
available abnormal Cholesterol person having age 45 year or more who did lipid
profile test and angiography, Who having no blockage in main blood vessels and do
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not have tendency to do bypass surgery and angioplasty on him or obese person. Lazy
whose life style is Luxury or without hard work, diabetic person one whose have
heredity of heart disease Cholesterol person selected in Nagpur municipal corporation
only.
25 men and 25 women applying controlling program of selected abnormal
cholesterol person and after one year conduct lipid profile test and data collected then
necessary statistical techniques applied to find the To study Effect of Selected
Exercise Module and Food Package on Cholesterol Level of Affected Individuals.
The research scholar use mean, standard deviation and t-test for interpreting the
results.
Criterion Measure and Administration of Test
For determination of the Cholesterol level or lipid profile test, estimate with
the CHOD/PAP ( CH- Cholesterol, OD- Oxides, P- Phenol, A- Amino antipyrine and
P-Peroxide ) most popular color metric methods The blood sample of the subject
taken before 10 to12 hours of fasting in the morning session.
1) The blood sample of the subject collected with the help of clinical laboratory
technician by using disposable syringe.
2) 2 ml blood sample collected from the subject
3) Blood sample taken in 5 ml test tube and allow to clot blood sample.
4) The blood sample centrifuged for 5 min till the serum from R.B.C. are separated
5) Then blood samples stored at 2.8 c and the level of Cholesterol estimated in
pathological laboratory.
6) Estimating total Cholesterol level
i)
1000 μml Cholesterol reagent taken and add 10 μml serum sample to it.
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ii)
Take 1000 μml Cholesterol reagent and add 10 μml Cholesterol standard in to
test tube
iii)
It incubated it at 37 c for 10 min.
iv)
After incubation taken OD (Optimal Density) of test and standard against
reagent blank.
v)
Total Cholesterol calculated through following formula
Abs. of test
Total Cholesterol in Mg/DL = Abs of Standard x 200
7) Estimating of Triglyceride level
i.
1000 μml Triglyceride reagent taken and add 10 μml serum sample to it.
ii. Take 1000 μml Triglyceride reagent and add 10 μml Triglyceride standard in to
test tube
iii. It incubated it at 37 c for 10 min.
iv.
After incubation taken OD (Optimal Density) of test and standard against
reagent blank.
Total Triglyceride calculated through following formula
Abs. of test
Triglyceride in Mg/DL = Abs of Standard x 300
8) Estimating of HDL level
Step –ii
i.
1000 μml HDL reagent taken and add 50 μml serum sample to it.
ii. Mixed well and incubate at room temperature for 5 minute.
iii. The above tube centrifuged at 2500-3000 rpm. From centrifuge machine
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iv. To all clear supernatant form above solution.
Step –ii
i. Take 1000 μml HDL reagent and add 50 μml Supernatant from step-i
ii. Take 1000 μml HDL reagent and add 50 μml Standards in to test-tube
iii. It incubated it at 37 c for 10 min.
iv. After incubation taken OD (Optimal Density) of test and standard against reagent
blank.
HDL calculated through following formula
Abs. of test
HDL Cholesterol in Mg/Dl = Abs of Standard x (25 x 2)
9) Estimating of LDL level
LDL Cholesterol level calculated with the help of total cholesterol,
Triglyceride and HDL through following formula.
LDL Cholesterol in Mg/Dl = Total cholesterol-{
Triglyceride
5
} - (25x 2)
11 Then estimating of VLDL level
With the help of LDL level through following formula
LDL Cholesterol in Mg/Dl =
Triglyceride
5
12. The estimated total cholesterol, Triglycerides, LDL VLDL, HDL Cholesterol
value compare with normal reference values as fallow.
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Types of Cholesterol
Values
Category
Total Cholesterol
Less than 200
Desirable
Mg/dl
LDL Cholesterol
200-239
Borderline
240 and above
High
Less than 100
Optimal
Mg/dl
HDL Cholesterol
Triglycerides
100-129
Near Optimal/ above optimal
130-159
Borderline High
160-189
High
190 and above
Very High
Less than 40
Low ( High Risk )
60 or highest
High ( Optimal)
Less than 150
Optimal Goal