Supporting information
Figure S1 Map of study location. Numbers indicate site locations.
2
4
5
1
3
Table S1. Geologic information for study sites. Information comes from the Secretary of
Industry, Commerce, and Mining for Argentina (1997), except in site 2, where higher
resolution information was available from Cucchi &Leanza (2006).
Site
Location
Description of parent material
Age
1
40º 15´ S
Colluvial and alluvialdeposits
Quaternary
39º 57´ S
Continental
Tertiary
71º 06´ W
sedimentsassociatedwithvolcanicactivity,
70º 48 W
2
representedbyandesitictuffs, partlydacitic,
alongwithtuffites
3
4
5
40º 26´ S
Mesosilic and basicvolcanite. Epi and
71º 13´ W
pyroclasticsediments.
40º 04´ S
Granodiorites, tonalitegranite,
71º 20´ W
rhyolitedikes
40°09´S
Mesosilic and basicvolcanite. Epi and
71°34´W
pyroclasticsediments.
Tertiary
Carboniferous
Tertiary
Table S2 Plant species from which leaves and ectomycorrhizae were collected along the
precipitation gradient in Patagonia, Argentina.
Native vegetation
Site
Leaves collected
Pine plantations
Ectomycorrhizae
Leaves collected
collected
Ectomycorrhizae
collected
1
Pappostipa spp., Mulinum spinosum
none
Pinus ponderosa
P. ponderosa
2
Pappostipa spp., Mulinum spinosum
none
P. ponderosa
P. ponderosa
3
Nothofagus antarctica, Pappostipa spp.,
N. antarctica
P. ponderosa
P. ponderosa
Mulinum spinosum
4
N. antarctica
N. antarctica
P. ponderosa
P. ponderosa
5
N. dombeyi, N. nervosa, N. obliqua,
N. dombeyi, N.
P. ponderosa
P. ponderosa
Chusquea culeou
nervosa, N. obliqua
Table S3 ANOVA results for evaluation of mean annual precipitation (MAP), vegetation
(Native or Pine Plantations) and plant part (leaf or ectomycorrhizae (ECM) and leaf and soil
values of C and N and stable isotope signatures. P values are marked in bold when
significant.
Leaf and ECM δ15N
Factor
MAP
Vegetation
Plant part
MAP x vegetation
MAP x plant part
vegetation x plant part
MAP x vegetation x plant part
F
32.023
16.103
88.848
0.005
0.457
0.746
0.493
p
< 0.001
< 0.001
< 0.001
0.946
0.501
0.391
0.485
Total soil C
Factor
MAP
vegetation
MAP x vegetation
F
102.922
8.188
0.091
p
< 0.001
0.007
0.765
Total soil N
Factor
MAP
vegetation
MAP x vegetation
F
112.394
16.107
0.018
p
< 0.001
< 0.001
0.893
Soil C:N
Factor
MAP
vegetation
MAP x vegetation
F
53.139
0.055
0.428
p
< 0.001
0.815
0.517
Soil δ15N
factor
MAP
vegetation
depth
MAP x vegetation
MAP x depth
vegetation x depth
MAP x vegetation x depth
F
0.883
3.753
254.398
0.008
0.251
2.084
0.155
p
0.349
0.055
< 0.001
0.930
0.617
0.152
0.695
Soil δ13C
factor
MAP
F
259.54
p
< 0.001
vegetation
depth
MAP x vegetation
MAP x depth
vegetation x depth
MAP x vegetation x depth
9.045
240.962
8.433
2.744
7.296
5.961
0.005
< 0.001
0.003
0.100
0.007
0.020
Table S4 Soil δ13C by depth ± standard error, in sites with native vegetation and pine
plantations, along the precipitation gradient.
Native vegetation
Mean annual precipitation (mm)
Depth (cm)
250
650
1100
1350
2200
0-5
-26.1
±
0.0
-26.4
±
0.1
-26.8
±
0.1
-27.0
±
0.1
-27.0
±
0.1
5-15
-25.2
±
0.1
-25.5
±
0.1
-26.1
±
0.1
-25.6
±
0.0
-26.2
±
0.2
15-30
-24.5
±
0.0
-25.0
±
0.0
-25.3
±
0.2
-25.3
±
0.0
-26.1
±
0.1
30-60
-
-
-
-
-
-
-25.5
±
0.1
-24.9
±
0.1
-26.1
±
0.0
60-90
-
-
-
-
-
-
-25.5
±
0.3
-24.9
±
0.1
-26.0
±
0.1
Pine plantations
Mean annual precipitation (mm)
Depth (cm)
250
650
1100
1350
2200
0-5
-25.2
±
0.1
-25.9
±
0.1
-26.1
±
0.5
-26.2
±
0.0
-27.2
±
0.1
5-15
-25.1
±
0.1
-25.5
±
0.1
-25.7
±
0.1
-25.5
±
0.1
-27.0
±
0.1
15-30
-24.6
±
0.1
-25.0
±
0.0
-24.9
±
0.1
-25.2
±
0.1
-26.1
±
0.1
30-60
-
-
-
-
-
-
-24.7
±
0.1
-25.0
±
0.1
-26.0
±
0.1
60-90
-
-
-
-
-
-
-25.1
±
0.8
-24.6
±
0.1
-25.8
±
0.1
2
4
5
1
3