Ohio University
Heritage College of Osteopathic Medicine
Office of Research & Grants
Research & Scholarly Advancement Fellowship
Summer, 2015
APPLICATION DEADLINE: February 13, 2015
ACCEPTANCE NOTIFICATION: February 27, 2015
PROGRAM DATES: June 1-August 7, 2015
Contact: Jessica Wingett, Program Director (wingettj@ohio.edu)
Summary of Program
Since 1978, the Heritage College has sponsored a program to provide summer research experience for medical
students. The program seeks to match students who have successfully completed their first year of the
Heritage College curriculum (beginning second year medical students) with Heritage College faculty mentors
and Heritage College affiliates (e.g. affiliates within the OhioHealth, Nationwide Children’s Hospital or
Cleveland Clinic systems) who can encourage and supervise a meaningful experience in research or scholarly
activity.
This program provides research opportunities that fellows will pursue under Heritage College faculty or
Heritage College affiliate direction. Projects can include contributing to ongoing research in the laboratory or
clinic, or initiation of the student’s own project under Heritage College faculty or Heritage College affiliate
direction. In addition to the research project, fellows will be required to attend the program orientation,
seminars on various research topics, and the program closing session; submit project progress reports; and
prepare and present a poster or presentation on the project at the joint Heritage College/College of Health
Sciences & Professions research symposium during the spring semester following the fellowship program.
The fellowship pays $3,000 to assist fellows with living expenses over the ten-week program. Fellowship
payments will be paid intermittently throughout the program and will be paid through the Bursar’s Office,
located in Chubb Hall.
Upon successful completion of the fellowship program, fellows will receive one hour of academic credit. There
will be no tuition cost to the fellows for participation in the program and the program will be graded on a
pass/fail basis. Upon acceptance into the program, fellows will be registered for the fellowship with the
permission of the Office of Research & Grants.
Funding for up to nine RSAF fellowship awards (four from the Athens Campus; five from the Dublin Campus)
will be provided by the Osteopathic Heritage Foundations. Funding for up to eleven RSAF fellowship awards
will be provided by OU-HCOM. Additional RSAF fellowship awards may be made based on the availability of
funds.
Eligibility
All students who will successfully complete the Year 1 medical school curriculum by the end of spring
semester 2015 are invited to apply for the Research & Scholarly Advancement Fellowship (RSAF) program.
Students must also be in good professional and judicial standing at the time of application through the
completion of the program. This ten-week summer program is designed to introduce osteopathic students to
basic science and clinical research and scholarly activities under the guidance of an OU-HCOM faculty mentor
or affiliate.
Student Selection Process



Mentors will be provided with the applications for students who have expressed an interest in
working with the mentor and on his/her research project. After review of applications, mentors are
required to rank applicants in order of preference.
After all mentor rankings have been received, the Office of Research & Grants will then review
rankings and place students. We will make our best effort to place students according to the mentor’s
preference, but there is no guarantee. Due to the limitation of funds and competiveness of the
program, mentors are not guaranteed placement of a fellow for the summer. Final placement of
students will be made at the discretion of the Office of Research & Grants.
Exceptions to the student selection process will be made for students participating in the DO/PhD
program through the OU-HCOM Office of Advanced Studies. These students are not exempt from
the application process, but will be guaranteed placement within the RSAF program due to
their required participation as part of the DO/PhD program.
Conditions of Appointment
Appointment of a fellow is contingent upon successful completion of the Year 1 medical school curriculum by
the end of spring semester 2015 and that the student is in good professional and judicial standing at the time
of application through the completion of the program.
Fellows are expected to conduct research according to the highest scientific and ethical standards and in
compliance with all applicable laws, regulations, and policies regarding protection of human research subjects,
humane care and use of laboratory animals, and laboratory safety.
Application Process
1. Read the mentor/research project profiles provided. You must select only from mentors/research
projects included in this application package.
2. Contact the mentor(s) with whom you may be interested in working and talk with them about your
interests. Students must consult with potential mentors prior to applying to work with them.
Consultations must be conducted in person or via video conference, prior to submission of the
student application package. Students must also complete the Project Discussion Form (found
in the student application package) and have the form signed by both you and your proposed
mentor (or acknowledged via email to Jessica Wingett at wingettj@ohio.edu ) by the
application due date. One form must be completed for each faculty mentor/research project for
which you are applying.
3. Complete and return the RSAF application package (provided), no later than Friday, February 13,
2015. Application package includes:
 Document Checklist Form – Applicant must sign this form and insure that all documents are
included
 Program Application Form – Applicant must sign this form
 Driver’s License and Ohio University Identification Card – Copy of driver’s license and OU ID
card
 Résumé/Curriculum Vitae – Must include: academic history, employment history, research
experience, list of honors/awards received, and publications
 Project Discussion Form(s) – Applicant and Faculty Mentor(s) must sign this form (see
additional instructions above). One form is required for each project for which the student
applies.
Submission Procedures
Application Deadline: February 13, 2015
All forms must be submitted in one large envelope by the application deadline. Dublin Campus students may
send via Campus Mail. Please allow adequate delivery time, as no applications will be accepted after the
deadline date. Electronic applications will not be accepted. Please deliver application envelopes to:
Jessica Wingett
Office of Research & Grants
243 Irvine Hall
Athens, OH 45710
IMPORTANT DATES
Application Deadline
Acceptance Notification
Program Begins/Orientation Session
Research Seminars
Program Ends/Closing Session
February 13, 2015
February 27, 2015 (approximate)
June 1, 2015
June 1-5, 2015
August 7, 2015
For more information, contact:
Jessica Wingett, Program Director, at 740-593-2336 or wingettj@ohio.edu
Ohio University
Heritage College of Osteopathic Medicine
Office of Research & Grants
Research & Scholarly Advancement Fellowship
Summer, 2015
CHECKLIST
To the Applicant:
Please complete and sign below. Make sure to have ALL requirements in one sealed envelope. Incomplete
application packages will NOT be reviewed.
 Document Checklist Form – Sign below and include this form in application envelope
 Program Application Form
 Driver’s License and Ohio University Identification Card – Copy of driver’s license and OU ID card
 Résumé/Curriculum Vitae – Must include: academic history, employment history, research
experience, list of honors/awards received, and publications
 Project Discussion Form – Applicant and Faculty Mentor(s) must sign (or acknowledge via email
to Jessica Wingett at wingettj@ohio.edu) this form. One form is required for each project for
which the student applies.
I verify that I have included all of the above documents in my application package. I understand that if all of
the above documents are not included, my application will not be reviewed.
Name of Applicant: __________________________________________________________________________________________
______________________________________________
Signature of Applicant
___________________________________________
Date
Ohio University
Heritage College of Osteopathic Medicine
Office of Research & Grants
Research & Scholarly Advancement Fellowship
Summer, 2015
PROGRAM APPLICATION
General Information
Applicant’s Name (Last, First, Middle Initial)
Daytime Phone
Date of Birth (mm/dd/yy)
Evening/Alternate Phone
OU PID #
Email (OHIO)
Current Mailing Address (Street and Apt. or PO Box Number, City, State, Zip Code)
Permanent Mailing Address (if different than above)
Program Preferences
Choose mentor/research project in preference order (maximum 2 options). Must be
selected from the research mentors/projects included in this application package.
1.
2.
1. Describe honors, hobbies, special interests or previous work experience that you would
like considered. (use additional pages as needed)
2. Have you conducted research previously?
If yes, please describe:
3. Publications? (List)
YES
NO
4. Why did you decide to apply for this program?
program?
Use additional pages as needed
What are your goals as related to the
Certification & Eligibility Verification
I certify that the above information is accurate and true to the best of my knowledge. I give permission to the
Ohio University Heritage College of Osteopathic Medicine Office of Research & Grants to share this information
for the purpose of recruitment, placement, monitoring, and evaluation. I certify that I meet all the eligibility
criteria stated in the Research & Scholarly Advancement Fellowship Program Summary.
Name of Applicant: ___________________________________________________________________________________________
_______________________________________________
Signature of Applicant
___________________________________________
Date
Ohio University
Heritage College of Osteopathic Medicine
Office of Research & Grants
Research & Scholarly Advancement Fellowship
Summer, 2015
PROJECT DISCUSSION FORM
This form is designed to insure that students and potential mentors meet (in person or via video conference)
to discuss projects that interest students prior to application. This meeting will provide students and mentors
with an opportunity to meet outside of the classroom or clinical setting, discuss the project and the fellow’s
role in the project, and allow for questions. This form must be completed by the student, signed by both the
student and faculty mentor (or acknowledged via email to Jessica Wingett at wingettj@ohio.edu ) by the
application due date or included in the application package. One form must be completed and included for
each project for which the student applies.
Applicant Name
Mentor Name
Project Title
Meeting Date
1. Overall Objective/Goal of the Project
2. Role of Fellow in Project
3. Role of Fellow in Lab/Clinic
4. Other (if applicable)
_______________________________________________
Signature of Applicant
_______________________________________________
Date
_______________________________________________
Signature of Mentor
_______________________________________________
Date
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Fabian Benencia, PhD
Department of Biomedical Sciences
Academic & Research Center 202C
Athens, OH 45701
Phone: (740) 597-2133
Email: benencia@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Molecular biology techniques; Polymerase Chain reaction
2. Cell culture techniques (Sterile environment procedures)
3. Immunohistochemistry; Immunofluorescence
4. Literature review and notebook keeping
Project Title: Expression And Subcellular Localization Of Toll-Like Receptors In Breast Cancer Cells Upon
Induction Of Epithelial- To-Mesenchymal Transformation
Project Description:
The Epithelial-to-Mesenchymal Transformation (EMT) is a cellular process that polarizes immotile epithelial
cells and turns them into motile mesenchymal cells. Thus, the EMT endows cancer cells with migratory and
invasive capabilities needed during cancer metastasis. In breast cancer, the EMT is associated with overexpression of transcription factors(SNAIL, SLUG, TWIST, E-Cadherin, and SPARC) and a decrease in the
expression of N-Cadherin. Also, upon EMT cells show an increase in self-renewal capabilities and resistance to
conventional therapies. Taking all of these into account, it is not surprising that the EMT process is considered
a very important player in cancer malignancy. Research indicates that soluble molecules such as transforming
growth factor (TGF)-β, interleukin (IL)-6, or epithelial growth factor (EGF) are able to induce EMT in cancer
cells.
In the last decades, toll-like receptor (TLRs), have emerged as important molecules related to inflammatory
process. TLRs are a family of membrane-associated receptors expressed by immune cells that recognize
microbial (pathogen-associated molecular patterns, PAMPs) or cellular (Damage-associated molecular
patterns, DAMPs) products. Signaling through TLR can induce the production of cytokines and growth factors
by immune cells, helping them to eliminate microbial infections. In addition, in the last 10 years it has been
determined that these receptors are also expressed by tumor cells, although their function in these cells is still
not completely defined. Recently, it has been shown that there is relationship between signaling through some
TLRs and EMT in tumor cells. Activation of TLR4 or 5 in lung or hepatic cells was able to induce EMT in vitro
(1,2). Interestingly, it has also been shown that the subcellular localization of TLRs was modified in cancer
cells regarding to non-cancerous cells of the same ontogeny (3).
In lieu of these observations, we propose to investigate the levels of expression and subcellular localization of
TLRs in cancer cells under EMT. This will help better define the relationship between TLR signaling and the
EMT process in cancer. To accomplish this, human breast cancer cells will be subjected to EMT by a protocol
already in place in our lab. At different time points, cells will be analyzed at the level of RNA for the expression
of TLRs 1-9, and at the level of protein by means of immunofluorescence and/or immunohistochemistry. The
latter will allow us to determine a modification on the localization of these receptors in the cells. TLRs localize
to the plasma membrane or the membrane of endosomes, but some reports indicate relocalization to the
cytoplasm in tumors (3). Finally, cells under EMT will be also treated with TLR agonists to determine if they
are differentially activated respect to non-EMT controls. Activation will be evaluated by chemokine production
and by using NFK-β reporter systems.
1. Am J Physiol Lung Cell Mol Physiol 303: L1057-L1069, 2012
2. BMC medicine 2012, 10:98
3. Frontiers in Immunology, 2014, Vol5, Article 638
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Darlene Berryman, PhD
Department of Biomedical Sciences
Diabetes Institute
Konneker Research Labs
The Ridges, Room 108
Athens, OH 45701
Phone: (740) 593-9661 or (740) 593-9943
Email: berrymad@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Sample collection/isolation procedures – Student will become trained in handling potentially
biohazardous materials, experiment with fecal sample collection, and extraction methods for
DNA/RNA and metabolities from the sample will be optimized.
2. Diversity/Dynamics data analysis – The student will work with existing datasets to explore the
diversity and dynamics of the microbiota by exploring available bioinformatics and statistical tools.
3. Genomics work – The student will shadow staff at the genomics facility (either OU or OSU) to see large
scale sequencing options.
4. Clinical exposure/literature review – Student will work with a bariatric surgeon, Dr. Rana, from
OhioHealth, faculty in our Diabetes Endocrine Center, or faculty engaged in the CHIP program (Dr.
Drozek) to determine the feasibility of developing a dissertation project focusing on the microbiome
and their patient population. To do this, the student will evaluate the commonly collected clinical
measures in these practices and gain a better understanding of the patient population. Finally, the
student will use this information to formalize a dissertation project.
NOTE: This project was designed specifically for a DO/PhD candidate participating in the TBS program. NonDO/PhD students are not permitted to apply to work on this project.
Project Title: The relationship between the gut microbiome and nutrition on health and obesity
Project Description:
Recent evidence has shown that the gut microbiota has a significant influence upon an individual’s health.
Each individual’s gut contains various amounts of microbes, and it is this diversity of microbes that competes
for food and sites of colonization. A lack of this diversity allows harmful bacteria to populate the gut,
associating with and possibly contributing to an increased risk of chronic diseases, including inflammatory
conditions. Nutrition and lifestyle factors have been shown to influence this variation of microbiota found in
the gut. For example, studies that have examined the impact of whole grains and red meat upon intestinal
bacteria show that whole grain foods can increase the microbial diversity. Not only do short-term diet
changes, like eating whole grains or red meat, impact gut microbial growth, but long-term dietary patterns
have a significant influence as well. This can be seen especially in obese populations. Malnutrition and a
sedentary lifestyle, as seen with certain cases or obesity, can reduce the growth of certain populations of
bacteria, allowing more harmful microbes to settle and negatively affect the individual’s health.
How different diet patterns and lifestyle factors alter microbial growth and how the relationship between
nutrition and the microbiome influence health are yet to be completely understood. This project aims to
expose the student to methods that will be needed to complete a dissertation in this area. Specifically, the
student will learn the methods associated with collecting samples of gut microbiota or fecal metabolites,
isolating and sequencing DNA or RNA from fecal samples, and analyzing large datasets. The student will also
gain exposure to the sequencing methods available at the Ohio University genomics facility and potentially
other nearby institutions (e.g. Ohio State University). Finally, the student will become immersed in several
clinical practices or a nutrition intervention program (CHIP) to determine the feasibility of applying her
interest in the gut microbiome to these clinical populations. At the end of the summer, it is expected that the
student will have a developed dissertation topic and the initial skills to be able to address this topic. In
addition, the student will be asked to submit an external grant to relevant entities requesting funding for this
project.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Darlene Berryman, PhD
Department of Biomedical Sciences
Diabetes Institute
Konneker Research Labs
The Ridges, Room 108
Athens, OH 45701
Phone: (740) 593-9661 or (740) 593-9943
Email: berrymad@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Flow cytometry – will learn to isolate the stromalvascular fractions from adipose tissue and count
specific cellular population
2. Cell culture – will culture stromalvascular fraction from adipose tissue (so mainly preadipocytes) to
evaluate the secretory products from these cells including proteins and enzymes relevant to
extracellular matrix deposition and remodeling
3. Immunocytochemistry – will develop optimal methods for cyrosectioning and immunostaining in
adipose tissue.
4. Seahorse methodology – will participate in ongoing studies in the lab to assess the metabolic
activity/mitochondrial health of isolated adipocytes versus whole adipose tissues
5. Mouse handling/breeding – all trainees in the laboratory are exposed to new and ongoing mouse
technologies
NOTE: This project was designed specifically for a DO/PhD candidate participating in the TBS program. NonDO/PhD students are not permitted to apply to work on this project.
Project Title: Role of growth hormone in white adipose tissue fibrosis
Project Description:
Obesity is characterized by insulin resistance, inflammation and pathologically accelerated white adipose
tissue (WAT) remodeling. Although it has not yet been extensively studied, the extracellular matrix (ECM) of
WAT is intimately linked with these key features of obesity and is thus an important area of current obesity
research. The ECM is the structural framework of WAT and is made primarily of collagen fibers. An excess of
these collagen fibers, called fibrosis, has been observed in WAT with obesity. WAT fibrosis is thought to
contribute to the metabolic abnormalities and inflammation present in obese individuals. Growth hormone
(GH) has been shown to increase collagen in other tissues and is also known for its ability to impact WAT in a
depot specific manner. Preliminary data from our lab shows that the extracellular matrix in WAT is also
altered in bGH transgenic mice in a depot-, age- and sex-specific manner. Note that bGH mice are giant and
lean, yet have shortened life spans and exhibit characteristics normally associated with obesity, such as insulin
resistance. This contradictory phenotype – unhealthy leanness - allows us to examine WAT fibrosis
independent of normal adiposity and question whether the amount of ECM in the WAT is more important than
the overall amount of tissue. Our preliminary results demonstrate a novel role of GH in WAT metabolism and
suggest that ECM deposition likely contributes to the depot specific differences in WAT previously reported in
mice with modified GH action. During the summer, the student will augment these initial findings by several
alternative methods. Specifically, the student will participate in as many of the following related activities as
feasible: 1) evaluate the cellular source of collagen by isolating adipocytes from the stromalvascular fraction
(which contains preadipocytes, immune cells, etc); 2) evaluate the activity of enzymes relevant for ECM
degradation from a cell culture system; 3) explore a consistent method of cryosectioning and staining for WAT
and specific types of collagen; 4) determine the feasibility of using human adipose samples obtained via
collaborators for ECM abundance, and 5) explore the impact of excess ECM on metabolic/mitochondrial health
of preadipocytes/adipocytes. One of the projects will be chosen as the primary focus and the other portions of
the project will provide an opportunity for exposure to a variety of techniques though they may not be
completed during the RSAF program.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Jane Broecker, MD
Department of Obstetrics & Gynecology
75 Hospital Drive, Suite 260
Athens, OH 45701
Phone: (740) 594-8819 or (740) 818-6267 (Cell)
Email: broecker@ohio.edu
Project Classification:
Clinical Research & Social/Behavioral Research
Techniques and/or skills that the student will learn:
1. Data Analysis
2. Writing a research paper
3. Working with computer specialists
4. Informed Consent
5. Potentially, study design and the IRB process
Project Title: Internet Impressions of Long Acting Contraceptive Methods: College students' impressions of
internet based health information concerning contraceptive devices.
Project Description:
My ongoing research is about long acting contraceptives and our ongoing project will involve data analysis of
surveys administered by our past RSAF student, Robin Fuchs. We will be working with Joan Jurich to code the
responses and analyze the data collected. We will write a paper about our findings and how knowledge and
attitudes toward contraceptives may affect decision making about birth control, and how parental influences
affect patient attitudes.
My new study will be a study about how female (and possibly male) college students interact with the internet
to find information on short acting and long acting methods of contraception. This will involve working with
computer specialists, learning how to contact and consent patients for the study, and analysis of the data
collected. It has not yet been submitted for IRB approval, but I anticipate approval in the Spring.
I would also welcome a student to create his or her own study and go through the design, creation and IRB
approval of a simple study, if he or she desires.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Brian Clark, PhD
Department of Biomedical Sciences/OMNI
250 Irvine Hall
Athens, OH 45701
Phone: (740) 593-2354
Email: clarkb2@ohio.edu
Project Classification:
Clinical Research
Techniques and/or skills that the student will learn:
1. Medical Imaging Analysis (MRI, DEXA)
2. Human electrophysiology techniques (EMG, brain stimulation, etc.)
3. Clinical trial design and basic principles of clinical research
NOTE: Dr. Clark has multiple projects on which a student could participate. Dr. Clark will assign the RSAF
student to the study or studies of his choice, depending on need during the time period of the program. The
projects listed below are Dr. Clark’s ongoing projects to which the RSAF student could be assigned.
Project Titles & Descriptions:
The RELIEF Study: A phase 2 randomized controlled trial investigating the effectiveness and mechanisms of
action of manipulative therapy in low back pain.
The BEND Pilot Study: A phase 2 randomized controlled trial investigating the effectiveness of two different
exercise interventions at enhancing muscle size and function of the paraspinal muscles in patients with low
back pain.
The UNCODE Study: this study investigates the neurological contributors to muscle weakness in the elderly.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Karen T. Coschigano, PhD
Department of Biomedical Sciences
228 Irvine Hall
Athens, OH 45701
Phone: (740) 593-2196
Email: coschigk@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Animal (mouse) handling
2. Primary cell culture
3. Real-time RT/PCR
4. Immunohistochemistry
Project Title: Investigating the Molecular Basis of Diabetic Nephropathy
Project Description:
The focus of my research is to identify genes, proteins and regulatory pathways involved in the development of
diabetic nephropathy, with an emphasis on the roles of signal transducer and activator of transcription 5
(STAT5) proteins and inflammation. The goal is to someday aid in the design of more specific, targeted
markers and therapeutic approaches for the diagnosis, treatment or prevention of human diabetic kidney
disease. I am currently characterizing what appears to be a novel mouse model of diabetic tubulopathy, with
damage occurring in the tubulointerstitial region rather than the glomeruli of the kidneys. Damage in the
tubulointerstitial region is understudied and thus of high importance. Drs. Ramiro Malgor and Bob Hikida are
assisting with the morphological/pathological characterization and Dr. Lonnie Welch is helping with the
molecular characterization. We use a mouse line with mutated STAT5 genes and induce type 1 diabetes by
injection with streptozotocin. We are correlating pathology with changes in gene expression in the kidneys of
the mice using immunohistochemical or immunofluorescent methods, real-time RT/PCR and western blot
analyses in order to develop hypotheses regarding the molecular mechanisms of diabetic kidney damage. I am
also trying to create an in vitro cell culture system to more easily test the hypotheses generated during our
studies. Depending on the student’s interest, we will together design a project that contributes to the overall
research goals of the lab. Interested students can contact me at coschigk@ohio.edu to further discuss possible
projects.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
David Drozek, DO
Department of Specialty Medicine
106 Parks Hall
Athens, OH 45701
Phone: (678) 447-2509
Email: drozek@ohio.edu
Project Classification:
Clinical Research
Techniques and/or skills that the student will learn:
1. Data analysis
2. Literature review
3. Preparing an abstract, poster and paper draft
NOTE: Dr. Drozek has multiple different projects available. Students must select the specific project in which
they are interested in working on the application form. Funding to place students in more than one project is
not guaranteed.
Project Title #1: Effect of Email on the Complete Health Improvement Program (CHIP) Six Month Follow Up
Results
Project Description:
Participants of CHIP in Athens County, Ohio will be asked to participate in a study on the effect a weekly
follow-up email will have on their six-month health screen results. CHIP routinely performs a health screen at
baseline, at about the eleventh session and again at 6 months post graduation. Each health screen consists of
the CHIP health inventory questionnaire, which asks about lifestyle practices, and measurement of biomarkers
to include weight and fasting blood lipid profile and glucose.
Willing subjects will be randomized into two groups. The control group will receive one email following
graduation, congratulating them, and thanking them for study participation. The study group will receive a
weekly email with a link to Survey Monkey to take a survey and provide them with a short educational
experience via a link to another site where they can read an article or watch a video on a lifestyle medicine
topic.
Statistical analysis will be performed to determine the effect of a weekly email on the subject’s adherence to
lifestyle changes learned in CHIP and the effects on their weight, lipids and glucose.
The objective of this study is to evaluate the effect of weekly email communication on the adherence to
lifestyle change and consequent effect on biometric markers of chronic disease. The hypothesis is that email
communication will increase the likelihood of participation in the 6-month follow up, and that the results of
those who receive email will be better than those who do not receive regular email communication.
A study of several weight loss programs, including the Ornish program, which has similarities to CHIP,
demonstrated low rates of adherence and persistent weight loss at 1 year. Another study documented positive
persistence of lifestyle behavioral changes in CHIP participants at 18 months but did not document
biomarkers. A study of CHIP in New Zealand demonstrated persistence of improved biomarkers at three or
more years in the 37% of graduates that returned for evaluation. Our initial study of the immediate results of
the first 6 CHIP classes in Athens demonstrated significant improvement in biomarkers.
Work to be done:
The student may be involved in:
1) Literature search on the use of email to motivate / maintain compliance in patient treatment.
2) Data collection and entry
3) Statistical analysis of data with statistician
4) Preparing abstract and poster
5) Writing initial draft of a paper for publication
Project Title #2: Complete Health Improvement Program (CHIP) Risk Reduction/Claims Evaluation Project
Project Description:
In our Western culture, lifestyle changes focusing on diet, exercise and tobacco could prevent about 40% of all
cancer deaths, and 82% of cardiac deaths, in the U.S. It is estimated that 71% of colon cancers, 70% of strokes,
and 91% of diabetic cases could be avoided by living a healthy lifestyle. 1 These health problems add a
tremendous burden to our healthcare budget, and to the loss of productivity of our society. In 2007, it was
estimated that 2.3 trillion dollars was spent on healthcare in the U.S., $7,600 for each individual. 2 Expectations
are that without dramatic change, this cost will continue to increase to unsustainable levels.
The Complete Health Improvement Program (CHIP) is a community based lifestyle medicine program with
proven effectiveness in addressing these problems.
The project proposes to provide CHIP to adult (non-pregnant) Ohio University employees (and/ or their adult
family members) with diabetes / prediabetes, obesity / overweight, hypertension / prehypertension,
atherosclerotic cardiovascular disease, or dyslipidemia in an effort to improve self-management and the
consequences of biometric factors that can be modified by lifestyle changes. The CHIP program is an
educationally based, lifestyle intervention program that aims to reduce healthcare cost, absenteeism, and
increase employee productivity. We expect that participants following the programs guidelines will lower
their body mass index, cholesterol, reduce blood pressure and blood glucose levels, and therefore help to
prevent chronic disease.
Ohio University HR will provide research participants with scholarships to attend the CHIP program. One aim
of the project is to compare biometrics factors (weight, cholesterol, LDL, HDL, triglycerides, blood pressure,
and fasting blood glucose, HgA1c) of participants before and after completion of the program (program
completion defined as those who attended at least 14 of 16 CHIP classes, or 15 of the 18 new CHIP+ classes). A
second aim is to compare this group’s health claims (health care utilization—office visits, emergency room
visits, hospitalizations, medication costs) with a control group’s data (OU employees who have diabetes /
prediabetes, obesity / overweight, hypertension / prehypertension, atherosclerotic cardiovascular disease, or
dyslipidemia and do not participate in CHIP program) and a third aim is to compare the treatment groups’
absenteeism due to illness data with that of the control group.
Work to be done:
The student may be involved in:
6) Literature search on corporate lifestyle intervention programs
7) Data collection and entry
8) Statistical analysis of data with statistician
9) Writing case studies
10) Preparing abstract and poster
11) Writing initial draft of a paper for publication
1
2
Aldana, Steven G., The Culprit and the Cure, Chpt. 1, Maple Mountain Press, 2005,
Vedro, Peter J, Absolute Advanatage, Vol 3, #8, 2007, pg 9.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Jen-Tzer Gau, MD, PhD
Department of Geriatric Medicine & Gerontology
355 Grosvenor Hall
Athens, OH 45701
Phone: (740) 593-2136
Email: gau@ohio.edu
Project Classification:
Clinical Research
Techniques and/or skills that the student will learn:
1. Developing skills for case series study
2. Literature review and be familiar with clinical study design
3. Descriptive statistical data analysis and reporting
4. Writing the abstract and preparing manuscript in submission for publication
NOTE FROM MENTOR: My research interest is broad in spectrum, ranging from nutrition and health, case
series study or case reports. My current interest is to investigate the association between nutritional status
and fracture risk in older adults, particularly on the role of magnesium in bone health (osteoporosis status and
fracture risk). For the research projects that are specifically for medical students during the fellowship period,
there are potential projects that can be developed. Currently, these potential projects are:
Project Title #1: Abdomen imaging study findings and sleeping habit—case series study (new).
Project Description:
Abdominal discomfort and bowel health issues are common in the elderly. From my clinical observation, older
adults who complained of right abdomen discomfort without the evidence of acute cholecystitis and had
abdomen x-ray or CT scan evaluation, the right colon is often filled with significant amount of stool with colon
dilation, which may explain their clinical symptoms. Further investigation revealed that these patients have
the habit of sleeping on their right side. A case series study will be conducted to reveal the possible
relationship between right colon stool stasis with dilation and the sleeping habit.
Furthermore, our previous study of abdomen x-ray scoring system in analyzing the severity of stool retention
also revealed that patients with significant higher score of stool retention in the right colon will predict the
overall risk of clinical constipation. The data is available for data analysis for a potential student who is
interested in carrying out the statistical analysis of the existing data.
Project Title #2: A case series study of fracture cases who were hospitalized in a community hospital from
2006-2012 (ongoing project).
Project Description:
Data collection is completed and will need statistical data analysis and preparation of manuscript for
publication.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Jody M. Gerome, DO
Department of Obstetrics & Gynecology
Department of Medical Education
OhioHealth O’Bleness Hospital
55 Hospital Dr.
Athens, OH 45701
Email: geromez@ohio.edu
Project Classification:
Clinical Research
Techniques and/or skills that the student will learn:
1. Experience in extracting clinical data from electronic medical record (EMR)
2. Data and database management, collection and maintenance
3. Data analysis and manuscript/poster preparation
4. Clinical observation/shadowing – Collecting EMR data in clinical setting; shadowing physicians in
busy OB/GYN practice
Project Title: Screening and Diagnosis of Gestational Diabetes
Project Description:
A diagnosis of gestational diabetes mellitus (GDM) is well known to increase the incidence of adverse
pregnancy outcomes for both moms and babies. Maternal complications can include hypertensive disorders,
increased risk for cesarean section and birth trauma. Infants have an increased likelihood of being large for
gestational age, to suffer from jaundice and hypoglycemia and are more prone to operative delivery, shoulder
dystocia and birth trauma. At this time, there is not a consensus with regards to the optimal testing used to
determine the diagnosis of GDM. Even though complications are well known, and many feel that diagnosis
strategies at this point are inadequate, current opinion suggests that further research should be done before
embracing any newly endorsed diagnostic paradigms.
This project will be retrospectively collecting data exploring the outcomes of pregnancies where the mothers
were diagnosed with gestational diabetes (GDM) by two different screening mechanisms. Approximately 150
participants have been consented and approvals have been obtained to collect the data. Under the guidance of
the principal investigator and co-investigator (Lucy Bucher, DO), the 2015 RSAF student will be the primary
lead for data extraction from the electronic medical record (EMR) based in our clinical offices; managing the
data and database; perform the data analysis; and prepare manuscript and poster. As the data collection and
analysis progresses, the student may have the opportunity to develop sub-studies given the volume and
complexity of the data collected. The sub-study would be dependent on PI’s interest, IRB approval, and
progress on primary research project.
Ohio University Heritage College of Osteopathic Medicine’s Primary Care departments have developed a
Maternal, Infant and Child (MIC) research collaborative. This collaborative, comprised of OB/GYNs;
psychologists, nurses, Pediatricians, informatics faculty, public health professionals, and physical therapists, is
designed to generate collaborative research projects and initiatives. RSAF student will also have an
opportunity to participate in these research meetings and potentially contribute to MIC collaborative projects.
The level of involvement will be dependent on student interest and progress on primary project.
Individuals who participate with this investigator will be given ample opportunities to experience
collaborative, community-based research as well as shadowing clinical care in a busy OB/GYN practice.
Expected outcome of project: Abstract, poster and potentially manuscript for publication
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Zelalem Haile, PhD
Department of Social Medicine
6775 Bobcat Way, Room 450
Dublin, OH 43016
Phone: (614) 793-5591
Email: haile@ohio.edu
Project Classification:
Clinical Research
Techniques and/or skills that the student will learn:
1. Evaluating research methodologies that can be applied to large secondary data
2. A working understanding of database construction and manipulation
3. Analyzing data elements in a variety of data sets
4. Utilizing existing data to answer a research question
Project Title: Association between prenatal maternal characteristics, gestational diabetes and postnatal
feeding practices
Project Description:
In the United States, despite improvements in the overall rates of initiating breastfeeding, rates for
breastfeeding duration remain low. In order to achieve national breastfeeding objectives it will be necessary to
examine maternal characteristics that may be linked to breastfeeding outcomes across different subgroups.
Recent preliminary findings have identified that women with a history of gestational diabetes mellitus have
different breastfeeding outcomes. However, these findings need to not only be confirmed with additional
longitudinal studies. A comprehensive understanding of the role of maternal prenatal characteristics on
postnatal feeding practices is necessary to inform public health practice and policy. Using the prospective
observational cohort data from Infant Feeding Practices Study II and its Year Six Follow-Up, this study will
examine (a) factors associated with prenatal intention to initiate breastfeeding and continuation of
breastfeeding and how these factors differ by maternal diabetes status; and (b) the association of prenatal
weight gain and the presence of gestational diabetes mellitus (GDM) on patterns of breastfeeding outcomes
(initiation, frequency, and duration). Upon completion, this project has the potential to provide health
educators, healthcare providers, and policymakers specific information on prenatal and postnatal maternal
characteristics and health behaviors that need to be addressed to improve breastfeeding rates.
Students will have the opportunity to learn the use of large population-based secondary data in research.
Students will utilize a wide range of statistical software and learn to apply appropriate data management and
analytic strategies to existing data. Students will be receiving intensive introduction to epidemiological
concepts and methods and will be expected to actively engage in, collaborate in, or interpret the results of
these epidemiologic studies.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Gillian Ice, PhD
Department of Social Medicine
301 Irvine Hall
Athens, OH 45701
Phone: (740) 593-2128
Email: iceg@ohio.edu
Project Classification:
Social Science Research
Techniques and/or skills that the student will learn:
1. Literature Review
2. Manuscript preparation/manuscript review
3. Statistical analysis and interpretation
4. Grant writing
Project Title: Cross-national comparisons of the impact of family caregiving.
Project Description:
The reported impact of caregiving on the caregiver’s well-being is frequently negative in US-based studies, but
more varied globally. An estimated one billion people of all ages are disabled and approximately 140 million
orphans globally with many of these individual likely needing some level of assistance (Thrush and Hyder
2014; UNICEF 2014; WHO and The World Bank 2011). Although the burden of care often falls to family
members worldwide, this is especially true in low- and middle-income countries (LMICs) where formal care
alternatives are often scarce and cultural norms about family care are very strong. I am currently working on
analyzing two databases relating to older adults who are caring for ill or disabled adults and orphaned or
vulnerable children. The first is a 4 year longitudinal study in Kenya of older adults caring for orphaned
children. There are several outcome variables yet to be examined including nutritional intake, energy
expenditure and mental health. Additionally, these data can be used to test evolutionary hypotheses about
grandparental investment. The second data set was collected by WHO [(Study on global AGEing and adult
health (SAGE)] in six low and middle income countries and is a multifaceted aging study, which includes
caregiving data and multiple mental and physical health outcomes This study has data from China, India,
Ghana, South Africa, Mexico and Russia. With complete household data, it allows the examination of multiple
forms of caregiving and family dynamics. The objectives of this project are to compare caregiving
measurement in two ageing studies (SAGE and the US Health and Retirement Study), determine the impact of
caregiving on caregiver’s well-being and strengthen a multi-site research program on caregiving dynamics
across generations. Specifically, this project will 1) Compare prevalence of elder and child/younger adult
caregiving in SAGE and the US (HRS); 2) Determine impacts of caregiving on social connectedness, social
isolation and loneliness; 3) Compare and contrast relationships between caregiving and physical/mental
health within and between studies.
Students who work with me, will develop a research question to test with the data, conduct a literature search
and perform statistical analysis. Following the analysis, students will work with my collaborative team to
develop a manuscript for publication. Additionally, students may choose to work with me on a grant proposal.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Erin Murphy, PhD
Department of Biomedical Sciences
228 Irvine Hall
Athens, OH 45701
Phone: (740) 597-3061
Email: murphye@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Sterile technique
2. Genetic manipulation – gene detection and cloning
3. In vitro tissue culture based analysis of bacterial virulence processes
NOTE: Dr. Murphy has two different projects available. Students must select the specific project in which they
are interested in working on the application form. Funding to place students in both projects is not
guaranteed.
Project Title: Metagenomic-based detection of pneumonia causing bacteria from ventilator filters
Project Description:
Background: Ventilator-associated pneumonia is a common complication suffered by patients supported by
ventilators. Are study is designed to determine if we can detect pneumonia causing bacterial pathogens
present in the external filters of ventilation units. Such detection would allow for fast, early and importantly
non-invasive detection of ventilator-associated pneumonia before the patient shows signs of illness. The
currently funded project is “proof of concept” and aims to validate the technique prior to establishing a clinical
collaboration.
Objective: Determine if we can utilize 16s metagenomic-based analysis to reliably detect the presence of
bacterial pathogens seeded onto ventilator filter units.
Nature of the work to be performed: A series of experimental approaches will be utilized to determine if we
can utilize 16s metagenomic analysis to reliably detect bacterial pathogens seeded onto ventilator filters. The
project will involve seeding ventilator filters with pure or mixed bacterial culture, removing the culture from
the filter, isolating total bacterial DNA from the extract and working with the genomics center to carry out 16s
metagenomic analyses. The techniques that the RSAF scholar can expect to learn include:
 Sterile techniques in handling bacterial cultures
 Bacterial DNA isolation
 Polymerase Chain Reaction
 16s metagenomic analysis and associated data analysis
o Students need not have experience in these approaches prior to their research experience.
They will be trained during their program
The RSAF participant will join an active laboratory, participating in all lab activities including weekly lab
meetings and journal clubs.
Research & Scholarly Advancement Fellowship
Summer, 2015
Faculty Mentor and Research Project Profile
Erin Murphy, PhD
Department of Biomedical Sciences
228 Irvine Hall
Athens, OH 45701
Phone: (740) 597-3061
Email: murphye@ohio.edu
Project Classification:
Basic Science Research
Techniques and/or skills that the student will learn:
1. Sterile technique
2. Genetic manipulation – gene detection and cloning
3. In vitro tissue culture based analysis of bacterial virulence processes
NOTE: Dr. Murphy has two different projects available. Students must select the specific project in which they
are interested in working on the application form. Funding to place students in both projects is not
guaranteed.
Project Title: Characterizing the role of the small RNA RyfA in controlling virulence of the pathogenic
bacterium Shigella flexneri
Project Description:
Background: Research in the Murphy lab focuses largely on understanding the role that regulatory RNA
molecules play in controlling the virulence of Shigella species. Regulatory RNA molecules, including small
RNAs, function by varied molecular mechanisms to control the expression of specific target genes. Previous
studies in our laboratory have revealed that twin small RNAs RyfA1 and RyfA2 influence virulence-associated
processes in S. dysenteriae. Unlike S. dysenteriae, S. flexneri encodes only one copy of RyfA and its function in
this pathogenic species is completely unknown.
Objective: Determine the role of the small RNA RyfA in controlling virulence-associated processes in S.
flexneri.
Nature of the work to be performed: Background: Research in the Murphy lab focuses largely on
understanding the role that regulatory RNA molecules play in controlling the virulence of Shigella species.
Regulatory RNA molecules, including small RNAs, function by varied molecular mechanisms to control the
expression of specific target genes. Previous studies in our laboratory have revealed that twin small RNAs
RyfA1 and RyfA2 influence virulence-associated processes in S. dysenteriae. Unlike S. dysenteriae, S. flexneri
encodes only one copy of RyfA and its function in this pathogenic species is completely unknown.
Objective: Determine the role of the small RNA RyfA in controlling virulence-associated processes in S.
flexneri.
Nature of the work to be performed: A series of experimental approaches will be utilized to characterize S.
flexneri RyfA and to determine if this small RNA, like its counterparts in S. dysenteriae, impacts the virulenceassociated processes of eukaryotic cell invasion and cell-to-cell spread. The role of RyfA in these virulenceassociated processes will be experimentally evaluated using in vitro tissue culture based analyses with S.
flexneri strains lacking RyfA (mutant strain lacking ryfA) or producing increased levels of the molecule (RyfA
produced from an expression plasmid). Techniques that will be learned and utilized include:
 Sterile techniques in handling bacterial cultures
 Genetic manipulation: gene cloning and/or inactivation
 Bacterial growth analysis

In vitro tissue culture based analysis of bacterial invasion into eukaryotic cells and the spread of
bacterial from one eukaryotic cell to the next
o Students need not have experience in these approaches prior to their research experience.
They will be trained during their program
The RSAF participant will join an active laboratory, participating in all lab activities including weekly lab
meetings and journal clubs.