LIMITED TENDER # 04/2014
Sealed Quotation/Tenders quoting rates for the supply of stores as detailed below to National Institute for
Biotechnology and Genetic Engineering (NIBGE), FAISALABAD, are invited as under:-
Limited Tender #
04/2014
Item
Chemicals and Reagents etc
Reference File No.
Proc-5(259)/2014 /NIBGE
Date & Time of
Tender Opening
11-03-2014
at 1500 hours
The following terms and conditions have been laid down for suppliers for submitting their quotations to NIBGE, Faisalabad.
(I)
The quotations should be submitted showing description/ specifications/ drawings along with signatures of
dealer/ suppliers duly affixed with his seal. The suppliers are not allowed to change or modify the given
specifications.
(II)
Additional information & Literature may also be attached and position of availability of stores in stock,
indicated before signing the quotations. Samples may be attached, if required.
(III)
Quotation/Tender should be sent in sealed cover duly marked with File No. and opening dated. Quotations
received without the marking on the Envelope will not be considered.
(IV)
Quotation should normally be valid for a period of 90-days from the date of opening of quotations and it will
be presumed that the Firms/dealers will supply the items on credit.
(V)
The bill will be paid at the earliest possible, provided the supplied store is of the ordered’s specifications, in
serviceable condition & correct quality.
(VI)
All amendment/over writing of rates in quotation may please be attested failing which the quotations will not
be considered.
(VII)
The rates are to be quoted on F.O.R. NIBGE, FAISALABAD, basis.
(VIII)
In any condition contrary to these terms detailed above are laid down by the supplier, such condition would
not be acceptable or binding on NIBGE.
(IX)
Earnest Money @ 2% of the total quoted value (including 17% GST ) in the shape of Deposit-at-Call
duly affixed Revenue Stamps according to its value (Cheque /Pay Order/ Bank Draft/ Demand Draft
not acceptable) in favour of Accounts Officer, NIBGE, FAISALABAD must accompany the quotation.
The bids not supported with CDR OR with less amount earnest money shall be rejected at spot.
(X)
The quotations may be sent through POST/COURIER SERVICE to the undersigned OR by hand at R & I
(NIBGE) Faisalabad before due date. Quotations shall be received at 1430 hours and opened at 1500 hours.
(XI)
The Competent Authority reserves the right to accept/reject any of the quotations or part thereof without
assigning any reason.
(XII)
The quotations of those firms will be accepted which are registered with Sales Tax Department having Sales
Tax Registration which should clearly be mentioned on quotation.
(XIII)
The rates should be in full rupee inclusive of 17% General Sales Tax. If GST not mentioned as Exclusive or
Exempted in quotation, the quoted rates shall be considered as Inclusive of 17% GST. Any decimal figure
shown with quoted rates shall be considered as deleted/ignored.
NOTE: Writing as “GST as per Govt. rules” will also be deemed as misleading and shall be considered as
inclusive of GST.
 All the firms who are interested to participate must be registered with any academic or research
institute working under Government of Punjab and should not be black listed by any establishment of
Pakistan Atomic Energy Commission of Pakistan.
Admin Officer (Procurement)
NIBGE, P.O.Box # 577, Jhang Road, Faisalabad.
Ph: (041) 2651475-79/Ext. 201,206/(041) 2650818
Limited Tender No. 04/2014
1
Taq Master Mix (2x); Quantity; 1000 reactions
20 x 1.25 ml (for 1000 reactions of 50 µl each). The mix should have high sensitivity
Taq polymerase for efficient amplification of atleast6 Kb DNA fragments from
genomic DNA and upto 20 Kb from viral DNA. The amplified DNA fragments should
have 3’-dA overhangs for efficient ligation in TA vector. Capable of incorporating
modified nucleotides and supplied with nuclease free water. The specifications should
be easily accessible as a brochure with the quote/supply or internet search using
catalogue number.
2
Oligonucleotide primer synthesis. Primer length 17-80 bases long at 50nmol scale
and a minimum OD of 20. Provided as Desalted/Salt free pellet in screw cap 2 ml
polypropylene tube supplemented with the synthesis data sheet. The requirement per
demand will be raised for synthesis of single or multiple primers over a span of three
years period. Total requirement for the three years period would be greater than 1000
bases with primer lengths ranging between 17-80 nucleotides per primer.
Low electroendosmosisAgarose (LE Agarose) EEO (mr) <0.12 Gelling temperature
(1.5%) 35oC +/- 1.5 melting point 89 oC+/- 1.5. Gel strength (1%) > 1800 gm/cm2
moisture< 6% sulphate< 0.2%, Ash <0.2%;
StoragePowder stable at room temperature. Quantity 500g
Quality Control: should not have nonspecific Endonuclease, Exonuclease or RNAse
Taq DNA Polymerase (recombinant), Quantity; 500 Units (5 units /l) Thermostable
withhalf life more than 40 min at 95°C. Should generate PCR products with 3’-dA
overhangs and supplied with two buffers – 10X Taq Buffer with KCl and 10X Taq
Buffer with (NH4)2SO4 for testing at wide range of magnesium concentrations and
decreases unspecific priming. The 25mM MgCl2 should also be supplied. The enzyme
should be capable of incorporating modified nucleotides (e.g., biotin-, digoxigenin-,
fluorescently-labeled nucleotides). For routine PCR the amplification of DNA
fragments up to 5 kb should be possible. The enzyme should catalyzes 5' to 3'
synthesis of DNA with no detectable 3' to 5' exonuclease (proofreading) activity. It
should possess negligible or low 5' to 3' exonuclease activity. In addition, Taq DNA
Polymerase should exhibit deoxynucleotidyltransferase activity to add extra adenines
at the 3'-end of PCR products. One unit of the enzyme should catalyze the
incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in
30 min at 70°C. The error rate of Taq DNA Polymerase should not be more than
2.2x10-5 errors per nt per cycle, and the accuracy of PCR should be 4.5x104. The
enzyme must be devoid of E.coli DNA and non-specific amplifications must not be
obtained in a template free PCR. The specifications should be easily accessible as a
brochure with the quote/supply or internet search using catalogue number.
dNTPsmixFor use in molecular biology applications including PCR,real time and
RT. Purity should be greater than 99%. Should be free of human and E.coli DNA and
other contaminants from the source organism. The mix should be stable for about 100
or more freeze-thaw cycles. Concentration 10 mM each dNTP in the mix.
3
4
5