VEER BAHADUR SINGH
e-mail: vbsinghh@gmail.com
 +91-9958339492
+91-9350563010
Senior Research Fellow
Division of Biochemistry
Indian Agricultural Research Institute
New Delhi-110012
As research scientist, aspiring to explore and find new heights in the field of science through
novel experimental and analytical approaches.
Academia
Doctor of Philosophy (Bioscience)
2012
Jamia Millia Islamia (A Central University), New Delhi, India
Master of Science (Biotechnology)
2000-2002
62.3%
Specialization: Agricultural Biotechnology
Allahabad Agricultural Institute (Deemed University), Allahabad, India 62.3%
Bachelor of Science–Honors (Agriculture)
Specialization: Plant Science
C.S.J.M., University, Kanpur, India
1996-2000
62.87%
Research Experience
Doctorate Research:
Supervisor: Dr. V.G. Malathi
Duration: 2009-2012
Work Place: Advanced Centre for Plant Virology, Division of Plant Pathology,
Indian Agricultural Research Institute, New Delhi, India
Thesis title: “Studies on RNAi mediated resistance for the management of Mungbean yellow
mosaic India virus (MYMIV) in soybean”
Nature of work:
Soybean is highly proteinaceous among legumes and also good source of phosphorous, lecithin
and as well as water soluble vitamins. Soybean has importance in human dietary and industrial
use. Yellow mosaic disease (YMD) of grain legumes is a major constraint in the cultivation of
pulses and grain legumes in India. Yellow mosaic disease (YMD), the most destructive disease of
soybean, caused by the viruses belonging to the Genus Bogomovirus in the family Geminiviridae.
Yellow mosaic disease of soybean is caused by two viruses i.e. Mungbean yellow mosaic virus
(MYMV) and Mungbean yellow mosaic India virus (MYMIV). Viruses of family Geminiviridae have
typical geminate particle morphology, encapsidating single stranded circular DNA of 2.5 to 3.0 kb
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in size as genome. The viruses of the genus Bogomovirus infect dicotyledonous plants and are
transmitted by whitefly (Bemisia tabaci Genn.). MYMIV have bipartite genome designated as
DNA-A and DNA-B, each of about 2.7 kb in size. Soybean cultivation was difficult in northern
India during 70s, because of YMD constraint. To exploit that potential, it is essential to overcome
productivity constraints of which YMD plays a major role. For control of YMD in soybean, the
pathogen should be identified, and managed by developing resistance variety. To achieve this
goal, following objectives have been framed in this study.
1. Survey, collection and maintenance of yellow mosaic virus isolates infecting Soybean.
2. Molecular cloning of replication initiation protein gene and intergenic region of the virus
isolates.
3. To study the variability among different isolates and identify the conserved region in the
replication initiation protein gene and intergenic region.
4. Development of RNAi constructs targeting replication initiation protein gene and intergenic
region.
5. To develop transgenic soybean plants with RNAi construct and their analysis.
The YMD infected leaves were collected from major soybean growing region of India. The
replication initiation protein gene (Rep, ORF AC1) and intergenic region (IR) were PCR amplified
using specific primer. The antisense construct of Rep gene and hairpin constructs for Rep and IR
region were made. Three Indian cultivars were transformed by agrobacterium-mediated
transformation and regeneration protocol standardized.
The salient findings of the study are summarized below:
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Eight YMD infected samples were collected from different geographical region of India.
Primers have been designed for antisense Rep, hairpin construct of Rep and IR region on the
basis of conserved region.
Two RNAi construct targeting Replicase gene (ORF AC1, antisense and hairpin) and one
targeting intergenic region (IR, hairpin) of MYMIV-Sb was made in plant transformation vector
and confirming through sequencing. The efficiency of this RNAi construct was tested for
mitigation of disease by co-agroinoculation along with the infectious clones of MYMIV-Sb. In
the case of plants co-agroinoculated with RNAi construct, the symptom severity as well as the
percentage of infection was almost negligible, confirming the inhibition of disease
development by RNAi construct.
Transformation protocol was standardized for three Indian cultivars. Fourteen T1 lines were
confirmed for antisense Rep gene, thirteen T0 lines for hairpin Rep and eighteen T0 lines for
hairpin IR gene incorporation.
Bioassay experiment were performed for 176 T1 antisense Rep transgenic plants, 143 lines
were expressed resistant for the MYMIV.
RNAi construct made is effective against the virus inoculation. First in soybean, the proof of
concept of RNAi has been shown in this study.
Post Graduate Research:
Supervisor: Dr. Radha Rama Devi
Duration: 2000-2002
Work Place: Diagnostic Lab, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India
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Thesis title: “Population based study of Spinal Muscular Atrophy using PCR based RFLP”
Nature of work:
Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease,
characterized by deletion in the exons 7 & 8 of SMN gene (188 bp and 187 bp respectively) of
the SMNt gene mapped on the 5q11.2 to 13.3 chromosome. A first time pilot study was
undertaken to asses SMA incidence in neonatal population of Indian using PCR based RFLP
analysis as a tool.
The salient findings of the study are summarized below:
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DNA of blood samples from 67 neonates and 15 referred cases were analyzed for detection
exons 7 & 8 of SMNt gene. PCR of exons 7 & 8 of SMN gene and subsequent RFLP
standardized.
Of the 15 referred cases 3 were detected with deletion of 188 bp on exon 7 and 187 bp on
exon 8 of SMN gene. However no positive cases were detected in neonatal DNA studies.
The results of the study indicated that DNA analysis of larger neonatal population is required
for early detection, genetic counseling, prevention and therapy.
Current Research Profile:
Supervisor: Dr. Archana Sachdev
Currently working as Senior Research Fellow (15th February, 2012-Till date)
Project: “Use of RNAi technology in developing low phytate soybean and rice”.
Work place: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India
Nature of work:
Cereal grains and oilseeds contain large quantities of phytic acid, which provides myo-inositol
and phosphorus required during seed germination and seedling establishment. Phytic acid has
a negative impact on animal nutrition and the environment. The otherwise nutritionally
adequate phosphorus content of cereal grains and oilseeds is bound in phytic acid and
therefore not available to monogastric animals that lack sufficient phytase in their digestive
tract for optimal phosphorous nutrition. ATP binding cassette (ABC) transporter is a key
contributor to phytic acid accumulation in soybean seeds. Phyitc acid level can be reduced by
silencing the ABC gene which engineer the dominant, seed specific block in phytic acid
accumulation.
Key Performance area:
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RNAi construct were designed using ABC transporter gene to reduce the level of phytic acid in
developing seeds of soybean.
Agrobacterium mediated transformation using the RNAi construct and analysis of putative
transformants for gene incorporation.
Analysis the level of phytic acid by MegaZyme kit, modified colorimetric method and HPLC.
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Previous Research Profile:
Supervisor: Dr. V.G. Malathi
(I) Senior Research Fellow (23rd Sept., 2010-14 Feb., 2012)
Project: “Net Work Project on Transgenics in Crops (NPTC)”
Work Place: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural
Research Institute (IARI), Pusa Campus, New Delhi, India.
(II) Senior Research Fellow (3rd Sept., 2007-8th August, 2010)
Project: “Molecular analysis of virulence of tomato leaf curl viruses: role of satellite DNA β”
Work Place: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural
Research Institute (IARI), Pusa Campus, New Delhi, India.
Key Performance area:
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Molecular characterization of monopartite (DNA A and DNA β) and bipartite (DNA A and DNA
B) tomato leaf curl begomoviruses through Rolling Circle Amplification (RCA).
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Infectivity analysis through Agroinoculation of Partial Tandem Repeat constructs (PTR) of the
clones and through biolistic delivery of rolling circle amplified products, of tomato
begomoviruses.
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Screening of susceptible and resistance tomato genotypes against tomato begomoviruses
through whitefly (Bemisia tabaci) transmission.
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Construction of cDNA library of susceptible and resistant tomato variety, binding activity of
cDNA with satellite conserved region (Yeast one-hybrid system) and βC1 of betasatellite
(Yeast two-hybrid system) was analyzed.
Supervisor: Dr. D.V. Singh and Dr. Rashmi Aggarwal
(I) Research Associate (16th Nov., 2006- 3rd Sept. 2007) and Junior Research Fellow (4th
April 2005- 14th Nov. 2006)
Project: “Toxin and DNA based variability among the isolates of Bipolaris sorokiniana causing
spot blotch of wheat and induction of defense genes involved in host pathogen interaction”
Work Place: Fungal molecular and Plant pathology laboratory, Division of Plant Pathology,
Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, India.
Key Performance area:
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Bipolaris sorokiniana (teleomorph Cochliobolus sativus) is the causal agent of common root
rot, leaf spot disease, seedling blight, head blight, and black point of wheat and barley.
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Total 113 isolates of spot blotch were collected from 6 zone and characterized by different
molecular markers. Many molecular markers is applied in the present study like RAPD, URP,
ISSR, ITS and RELP. After studying the result, the each zone are preparing separate cluster.
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On the basis of these data SCAR marker is developed for the estimation of Bipolaris spp. The
SCAR marker has industrial importance and this marker is to be commercialize for the quick
detection of fungal disease.
(II) Senior Research Fellow (28th Nov. 2003- 3rd April 2005)
Project: “Development of bioformulation for control of leaf blight in wheat under rice wheat
cropping system”
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Work place: Fungal molecular and Plant pathology laboratory, Division of Plant Pathology, Indian
Agricultural Research Institute (IARI), Pusa Campus, New Delhi, India.
Key Performance area:
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Trichoderma species have been investigated as biological control agents against Bipolaris
sorokiniana and Fusarium graminiarum. There morphological differences among different
species and isolates of the same species.
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We used 27 Randomly Amplified Polymorphic DNA (RAPD)-PCR and ITS primers to estimate
genetic variation among isolates of Trichoderma reesei (2 isolates), Trichoderma viride (10
isolates), Trichoderma pseudokoningii (1 isolate) and Trichoderma harzianum (1 isolate).
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Polymorphisms were found between species. RAPD analysis suggested that, two strains of
Trichoderma viride (T V 5-2 & 2211) and two other strains of Trichoderma viride (TCT-10 &
T2) are 88% same. Amplification of the ITS region produced a single band of approximately
600 bp for all the isolates. Although there was differences in branching between dendogram
derived from RAPD supported the distinction of 4 Trichoderma species, with the T. viride
isolates grouping together and showing differences at an intraspecific level.
Working Advantages
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Expertise in cloning and understanding the genome organization of begomoviruses causing
diseases of national importance –Mungbean yellow mosaic India virus (MYMIV), Tomato leaf
curl viruses (ToLCVs)
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Efficient in making infectious constructs and in various screening techniques (agroinoculation,
agroinfiltration, biolistic delivery, sap inoculation and whitefly transmission) to identify the
resistance source to leaf curl and yellow mosaic begomoviruses
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Molecular characterization of Bipolaris sorokiniana using universal rice primer (URP) and
Trichoderma spp. Using RAPD primers. Development of SCAR marker for Bipolaris
sorokiniana. Pathogenesity test of Bipolaris sorokiniana on wheat cultivars.
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Disease Diagnosis and identification, Disease assessment and screening.
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Culturing and handling of fungal pathogen/biocontrol agent.
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Well conversed with NASH, Southern, Northern (siRNA detection) Western blotting and ELISA
techniques
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Expertise in plant tissue culture techniques of genetic transformation using A. tumefaciens for
micropropagation, callus initiation, maintenance and regeneration
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Contemporary Methodologies: Rolling circle amplification (RCA), Real-time quantitative
PCR analysis, PCR mutagenesis, post transcriptional gene silencing and silencing suppression
assays, cDNA library construction, Yeast one-hybrid system, Yeast two-hybrid system
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Bioinformatic skills: Primers designing by manual and using Primer 3.0 software; Analysis
of sequences using GENERUNNER, BioEdit, MEGA 5.0; Recombination analysis (RDP3)
Professional Experience
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Resource person for the Training on “Application of Biochemical and Molecular techniques
for characterization of Plant pathogens” organised by Centre for Advanced Studies, Division of
Plant pathology, Indian Agricultural Research Institute, New Delhi.
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Resource person for the Training on “Biological control of Plant Pathogens” organised by
Centre for Advanced Studies, Division of Plant pathology, Indian Agricultural Research
Institute, New Delhi.
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Resource person for the training on “Biocontrol Strategies for Management of Plant
Pathogens” organised by Centre for Advanced Studies, Division of Plant pathology, Indian
Agricultural Research Institute, New Delhi.
Training/Workshop attended
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One month training on Tissue culture, at Co-operative Rural Development Trust (CORDET),
Motilal Nehru Farmer’s Training Institute, Ghiyanagar, Phulpur, Allahabad from March 2000 to
April 2000.
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Two months training on monitoring the pollution level during Mahakumbh Mela in
Sangam at Allahabad, at Department of Chemistry, Allahabad Agricultural Deemed
University, Allahabad from January 2001 to February 2001.
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Three months training on Study of Cyanobacterial strains, at Department of Botany,
University of Allahabad, Allahabad from June 2001 to August, 2001.
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One day orientation program on Radioisotopes Use and Safety, at the Nuclear Research
Laboratory, Indian Agricultural Research Institute, New Delhi on 7th August, 2006.
Publications
Full research papers:
International publications:
1. Rashmi Aggarwal, V. B. Singh, Renu Shukla, Malkhan Singh Gurjar, Sangeeta Gupta and
Tilak R. Sharma (2010). URP-based DNA Fingerprinting of Bipolaris sorokiniana Isolates
Causing Spot Blotch of Wheat. J Phytopathol. 158 (4): 210-216.
2. Neha Tiwari, Padmalatha K.V., Singh V.B., Haq Q.M.I., and Malathi V.G. (2010). Tomato leaf
curl Bangalore virus (ToLCBV): Infectivity and enhanced pathogenicity with diverse
betasatellite. Arch. Virol. 155 (8): 1343-1347.
3. R. Aggarwal, S. Gupta, S. Banerjee and V.B. Singh (2011). Development of a SCAR marker
for detection of Bipolaris sorokiniana causing spot blotch of wheat. Canadian Journal of
Microbiology, 2011, 57:(11) 934-942.
4. Neha Tiwari, Veer B. Singh, P.K. Sharma and V.G. Malathi (2012). Tomato leaf curl
Joydebpur virus – a monopartite Begomovirus causing severe leaf curl in tomato in West
Bengal (Communicated).
5. Veer B. Singh, Q.M.I.Haq and V.G. Malathi (2012). A RNAi approach targeting Rep gene of
Mungbean yellow mosaic India virus to develop resistance in soybean (Communicated).
National publication:
6. Rashmi Aggarwal, V. B. Singh, M. S. Gurjar, Sangeeta Gupta and P. Srinivas (2009).
Intraspecific variations in India isolates of Bipolaris sorokiniana infecting wheat based on
morphological, pathogenic and molecular characters. Indian Phytopathology, 62 (4): 449460.
7. Rashmi Aggarwal, Sangeeta Gupta, V. B. Singh and Sapna Sharma (2011). Microbial
detoxification of pathotoxin produced by spot blotch pathogen Bipolaris sorokiniana
infecting wheat. J. Plant Biochem. Biotechnol, 20 (1): 66-73.
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Chapters in training manual:
8. V. B. Singh, Aradhika Tripathi, Kirti Bhalla and Renu. 2006. Isolation of fungal genomic DNA.
In: Training course on Advanced Techniques in Plant Disease Diagnosis and ManagementA practical manual. Center for Advanced Studies, Division of Plant pathology, Indian
Agricultural Research Institute, New Delhi pp- 10-12.
9. V. B. Singh, Vandana Sharma, Sangeeta Gupta and Kirti Bhalla. 2006. Quantification of DNA.
In: Training course on Advanced Techniques in Plant Disease Diagnosis and ManagementA practical manual. Center for Advanced Studies, Division of Plant pathology, Indian
Agricultural Research Institute, New Delhi pp- 13-15.
10. V.G. Malathi, Veer Bahadur Singh and P. Jyothsna, 2010. Viral genomics and Transgenic
development- A laboratory manual. Center of Advanced Faculty Training (CAFT),
Advanced centre for plant virology, Division of Plant pathology, Indian Agricultural
Research Institute, New Delhi pp- 10-12.
Book chapter:
11. Aggarwal Rashmi, Sangeeta Gupta and V.B. Singh, 2008. Secondary metabolites produced
by biocontrol agents and their role in plant disease management. In: Ecofriendly
Management of Plant Disease. (Eds. Shahid and Udit Narain). Daya Publishing House New
Delhi-35, pp 397-415.
Invited lectures/orals/posters:
National:
12. Siddiqui, Shahid Ali, Chattree, Amit and Singh, Veer Bahadur (2001). Impact of mass
Bathing on heavy metals, concentration, pH, and electrical conductivity of sacred river
during 'Mahakumbh 2001 at Allahabad. Oral presentation in National symposium on
Plant Diversity and Biotechnology. Department of botany, Patna University, Patna,
October 9-10, 2001: 10.
13. Ahammed, S. Khayum, Aggarwal Rashmi, Singh, D. V. and Singh V. B. (2004). Optimising
nutritional conditions for the mass multiplication of Chaetomium globosum, an efficient
biocontrol agent of Bipolaris sorokiniana. Poster presentation in National symposium on
Crop Surveillance: Disease Forecasting and Management. Division of Plant Pathology,
IARI, New Delhi February 19 – 21, 2004 : 103.
14. Aggarwal Rashmi, Singh, V. B., Gupta, Vishnu Kumar and Singh, D. V. (2005). Pathogenic
and Molecular variability in Bipolaris sorokiniana. Oral presntation in National symposium
on sustainable Pant Protection Strategies : Health and Environmental Concerns. Division
of Plant Pathology, Dr. Balashaheb Savant Konkar Krishi Vidyapeeth, Dapoli, Ratnagiri
(Maharastra) October 15-17, 2005 : 67.
15. Aggarwal Rashmi, Singh, V. B., Jahani, Mehdi, Tripathi, Aradhika (2005). Variability in
Bipolaris sorokiniana, causing spot blotch in wheat. Submitted for the oral session in
National Symposium on Fungal Biodiversity, Biotechnology and Bioinformatics
(NSFBBB). Sri Bhagawan Mahaveer Jain College, Centre for Post Graduate Studies,
Bangalore February 2 & 3, 2006: 32.
16. Aggarwal, R., V. B. Singh, Rajbeer Singh and Aradhika Tripathi (2006). Molecular
characterization of Trichoderma spp. by PCR-RAPD and ITS region amplification. Oral
presented in 58th Annual meeting Indian Phytopathological Society held at Siliguri, 31 st
Jan.-3rd Feb., 2006.
17. Aggarwal Rashmi, Singh V. B. and Aradhika Tripathi. (2007). “Analysis of genetic variability
in Bipolaris sorokiniana using Universal Rice Primer (URP)”. Oral presentation in 9 th
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Indian Agricultural Scientists and farmers congress, BIOVED, Allahabad. January 29 –
30, 2007: 77.
18. Aggarwal Rashmi, Aradhika Tripathi and Singh V. B. (2007). “Molecular characterization of
Tilletia indica usingh URP-PCR technique”. Poster presentation in National symposium on
Plant Diseases and its Management, Rani Durgavati University, Jabalpur (M.P.), January
16 – 18, 2007: 103.
International:
19. V.B.Singh, Neha Tiwari, Haq.Q.M.I., Jyothsna P., Richa S., Archana K and V.G.Malathi
(2009). Infectivity of a new begomovirus: Tomato leaf curl Aurangabad virus
(ToLCAuV). The 6th Solanaceae Genome Workshop, Nov 8-13,2009, Le Meridien, New
Delhi, India.
20. Neha Tiwari, V.B.Singh, Haq.Q.M.I., Jyothsna P., Richa S., Archana K and V.G.Malathi
(2009). Infectivity of Tomato leaf curl Bangalore virus. The 6th Solanaceae Genome
Workshop, Nov 8-13,2009, Le Meridien, New Delhi, India.
21. Haq.Q.M.I., Neha Tiwari., Jyothsna P., V.B.Singh, Richa S., Archana K and V.G.Malathi
(2009). Cloning and Infectivity of tomato begomoviruses through Rolling Circle
Amplication (RCA) of the genome. The 6th Solanaceae Genome Workshop, Nov 8-13, Le
Meridien, New Delhi, India.
22. Richa Shukla, Haq.Q.M.I., Neha Tiwari., V. B.Singh, Jyothsna P., Archana K and V.G.Malathi
(2009). RNA silencing suppressors encoded by beta satellite of tomato begomoviruses.
The 6th Solanaceae Genome Workshop, Nov 8-13,2009, Le Meridien, New Delhi, India.
23. Jyothsna P, Archana K., Haq.Q.M.I., Neha Tiwari, Richa Shukla, V.B.Singh and V.G. Malathi
(2009). Realtime PCR for the quantification of Tomato leaf curl virus in tomato plants
and its vector Bemisia tabaci. The 6th Solanaceae Genome Workshop, Nov 8-13,2009,
Le Meridien, New Delhi, India.
24. Sulaiman K Fadil, Jyothsna P, Haq.Q.M.I., Neha Tiwari, Richa Shukla, V. B. Singh, Archana.
K. and V.G.Malathi (2010). Universal primers based on conserved region of coat protein
for the detection of whitefly transmitted begomoviruses. International Conference on
“13th Congress of the Mediterranean Phytopathological Union”. June. 20-25, CRA-PAV
Plant Pathology Research Centre, Via C. G. Bertero, 22, 00156 Rome, Italy.
25. Neha Tiwari, V.B. Singh, P.K. Sharma and V.G. Malathi (2010). Tomato leaf curl Joydebpur
virus – a monopartite Begomovirus causing severe leaf curl in tomato in West Bengal.
Conference on Whitefly and Thrips Transmitted Viruses, Aug 27-28, University of Delhi
South Campus, New Delhi, India
26. Malathi V.G., Jyothsna P, QMI Haq, Neha Tiwari, Richa Shukla,V.B. Singh, Archana Kumari.
(2010). Pathogenicity of mono and bipartite Tomato leaf curl viruses in India.
Conference on Whitefly and Thrips Transmitted Viruses, Aug 27-28, University of Delhi
South Campus, New Delhi, India
Nuleotide sequences published:
27. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. 5137 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242474.
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28. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. bs05 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ286764
29. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. bs25 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ286763.
30. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. bs55 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242476.
31. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. bs18 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242475.
32. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. 5137 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.
33. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. 4922 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.
34. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sp. 4805 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.
35. Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sorokiniana strain 9 18S ribosomal
RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene,
and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene,
partial sequence. Accession No. DQ229952.
36.
Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sorokiniana strain 64 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal
RNA gene, partial sequence. Accession No. DQ229951.
37.
Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sorokiniana strain 27 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal
RNA gene, partial sequence. Accession No. DQ229950.
38.
Aggarwal,R., Jahani,M. and Singh,V.B. (2005). Bipolaris sorokiniana strain 27 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal
RNA gene, partial sequence. Accession No. DQ229950.
39. Aggarwal,R., Singh,V.B., Jahani,M. and Singh,D.V. (2005). Trichoderma viride strain 1433
internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete
sequence; and internal transcribed spacer 2, partial sequence, partial sequence.
Accession No. DQ217841.
40. Neha, T., Singh, V.B. and Malathi, V.G. (2010). Tomato leaf curl Bangalore virus isolate 18
segment DNA-A, complete sequence. Accession No. GU474418.
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Personal details
Date of birth
Father’s Name
Nationality
Marital status
Languages known
:
:
:
:
:
10th March, 1979
Mr. J.S. Singh
Indian
Married
English & Hindi
References:
Dr. V.G. Malathi
Emeritus Scientist (CSIR),
Plant Virology Unit,
Division of Plant Pathology,
Indian Agricultural Research Institute,
Pusa Campus,
New Delhi – 110012, India
vgmalathi@rediffmail.com
Phone No. +91-9717843963
Dr. Rashmi Aggarwal
National Fellow
Division of Plant Pathology,
Indian Agricultural Research Institute,
Pusa Campus,
New Delhi – 110012, India
rashmiiari@yahoo.com
Dr. D.V. Singh
Ex-Head,
Division of Plant Pathology,
Indian Agricultural Research Institute,
Pusa Campus,
New Delhi – 110012, India
Date:
Place:
(Veer Bahadur Singh)
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