1 1 SUPPLEMENTARY METHODS 2 Flow velocity data were collected using a 1200 kHz acoustic Doppler current profiler 3 (ADCP) (RD Instruments Rio Grande 600 kHz) housed within the autonomous 4 underwater vehicle (AUV) Autosub3. The velocity data were collected on Autosub3 5 mission M436 conducted from 22nd -24th May 2010. The data presented in this paper 6 are a subset of the data collected during this deployment. 7 The velocity data were processed using the following steps: i) position data 8 from the AUV’s inertial navigation system was corrected for drift using GPS fixes; ii) 9 vector data was rotated from a coordinate system relative to the AUV to a global 10 coordinate system; iii) the component of velocity resulting from movement of the 11 AUV was removed from the ADCP velocity iv) depth measurements were corrected 12 for vertical offset of the ADCP relative to the AUV’s pressure sensor; v) all data 13 below the depth of maximum ADCP backscatter intensity and within the acoustic 14 side-lobe blanking distance (B) of the seafloor were removed, using B = asin2θ, where 15 a is the altitude of Autosub3 and θ is the angle that the acoustic beam is emitted from 16 the ADCP (20°); vi) the magnitude and direction of the resultant velocity vector was 17 calculated from the three velocity components; vii) depth data were converted from 18 being relative to the submarine to relative to the sea surface. 19 Conductivity-temperature-depth (CTD) profiles were acquired from the Koca 20 Piri Reis. The two profiles presented in this study (stations 51 and 98) were collected 21 on the 19th May 2010 and 23rd May 2010, respectively. Fluid density was calculated 22 from the CTD data using UNESCO (1983). 23 As noted in processing step five, there is a blanking area near the seafloor 24 where the ADCP cannot measure velocities. We tried several methods for 25 interpolating the data in this region, assuming a no slip condition at the seafloor. As 2 26 the size of the blanking distance varies along the section and we do not know the 27 shape of the profile in this region we ultimately chose to use a linear interpolation for 28 its simplicity. It should be noted that regardless of how this data is interpolated the 29 Froude numbers calculated using this data will always be less than the Froude number 30 calculated using the maximum velocity rather than depth averaging the data. 31 The ADCP provides an estimate of the standard deviation in each velocity 32 measurements by evaluating the difference in values of velocity calculated using 33 different combinations of three of the four ADCP beams. For each vertical velocity 34 profile in the velocity matrix the mean error was calculated. Matrices of maximum 35 and minimum velocity measurements were then created by adding and subtracting 36 these errors respectively. The manufacturer’s resolution estimates for salinity (0.4 37 ppm) and temperature (0.0001C) were used to estimate the error in the density 38 measurement by propagating them through the equations presented in UNESCO 39 [1983], resulting in uncertainty in the density measurements of 0.08 kg/m3. These 40 errors were the propagated through the Froude equation to provide estimates of 41 maximum and minimum Froude number for each vertical velocity profile. 42 43 SUPPLEMENTARY REFERENCES 44 45 46 47 UNESCO (1983) Algorithms for computation of fundamental properties of seawater: Paris, UNESCO Division of Marine Science Technical Papers in Marine Science 44, 53p. 48 49