Fig. S1 Relative expression levels of VaPAT1 in transgenic Arabidopsis lines. The gene expression level was
analyzed by quantitative real-time PCR. Bars indicate standard deviation of three biological replicates. The three
overexpression lines (35S::VaPAT1-1, -2, -4) with higher VaPAT1 expression levels and used in subsequent
experiments were circled in red oval.
Fig. S2 MDA concentration, activity of SOD, POD and CAT of WT and VaPAT1 trangenic seedlings in
normal growth conditions or exposed to cold (4 °C for 3 d), drought (withholding water for 8 d) and salt (200
mM NaCl for 7 d) stress. Values were the means of three replicates and bars represent the standard errors (n = 3).
Table S1. Stress-related genes and their main functions
Gene
Functions in stress
AtSIZ1 (small ubiquitin-like modifier
(SUMO) E3 ligase)
AtCBF1
Modulate
expression,
Reference
stress-responsive
increase
genes
drought
and
freezing tolerance
(C-repeat/DRE
binding
Induce COR (cold-regulated) genes
factor 1)
expression , increase stresses tolerance
AtATR1/MYB34 (altered tryptophan
response to various phytohormones and
regulation 1)
salt stress
AtMYC2 ( bHLH transcription factor)
(responsive
dessication 29A)
Nakata et al. 2013)
to
various
expression
stresses,
increases
Response to various
abiotic stresses
via ABA-dependent and -independent
pathway
AtRD29B (responsive to dessication
Response to various abiotic stresses via
29B)
ABA-dependent pathway
AtProDH1
1)
(proline-dehydrogenase
(Chen et al. 2006)
various biotic and abiotic stresses
constitutive
to
(Novillo et al. 2007)
(Abe et al. 2003;
freezing tolerance
AtRD29A/COR78
Catala et al. 2007)
Response to ABA, JA (jasmonate) and
Response
AtCOR15A (cold-regulated 15A)
( Miura et al. 2007;
(Thalhammer et al.
2014)
(Miyazaki
et
al.
2015)
(Kim et al. 2014)
Catalyze proline metabolism, oxidative
(Miguel Cecchini et
and drought stress responsive genes
al. 2011)