Supplementary material
Figure S1: Apparatus for recording data for kinematic analysis. Each dog walked on the
treadmill (T), supported using a sling (arrow) positioned under the abdomen. Infrared
emitting and receiving cameras (C) positioned around the treadmill record motion of
retroflective markers affixed to various sites on the limbs.
Figure S2: Illustration of assessment of changes in locomotor activity following olfactory
ensheathing cell transplantation through a series of video recordings of dog (number 8 in the
trial) following intraspinal olfactory mucosal cell transplantation. The dog is walking on the
treadmill at the imposed speed of 0.42 ms-1 (1.5 kmh-1) with the body partially supported by a
belt placed under the abdomen to maintain the thoracolumbar spine parallel to the treadmill
surface. For the kinematic study, infra-red cameras were arranged around the treadmill (Fig.
S1) and their positions were unchanged between recordings. The left fore-hind coordination
(log) score was 2.71 before the intervention, 1.21 at one month, 0.73 at three months and 0.78
at six months, following intraspinal transplantation of olfactory mucosal cells. Although the
locomotor score remained stable from three months to six months, there was an alteration in
tail carriage between the two recordings.
Figure S3: Number of transplanted cells (y-axis) in each dog (x-axis) that received an
olfactory mucosa cell transplant. The red bars indicate the number of p75+ cells
(assumed to be olfactory ensheathing cells); the green bars indicate the number of
fibronectin+ cells (assumed to be fibroblasts derived from the sub-mucosa); the blue
bars indicate the number of unidentified cells.