VIRAL CLE AR ANCE SERVICES Equipment Request Form Complete this form and submit it to your account manager by email with the approved study quote. Information supplied on this form will assist in finalizing your study scheduling. Mark to indicate equipment being requested: 1.0 N2 Tank (includes coarse regulator with John Guest Male Connector NPTF Polypro - 1/4 x 1/4 NPTF PP010822W) coarse regulator is not calibrated 2.0 Peristaltic Pump 3.0 UV Spectrophotometer AND/OR NanoDrop 4.0 pH Meter AND/OR Conductivity Meter 5.0 Preferred number of units: (limited availability) Nanofiltration: Will a third-party filtration vendor will be performing your nanofiltration steps? No Yes (Filter vendors are responsible for supplying all process equipment for nanofiltration: pressurize feed vessels, pressure meters, fine adjust pressure regulators, tubing with corresponding fittings, balances, ring stands, data acquisition software, PC, etc.) 6.0 Balances: Analytic Balances (not portable) — used for determination of fraction volumes (0.01g) Portable — for use in Client-Company-performed nanofiltration steps (0.1g) Preferred number of units: (limited availability) – not applicable for third-party filtration vendors NOTE: Actual number of AKTA units allocated will be documented on the quote based on the viral clearance study design. 7.0 AKTA Explorer100 Preferred number of Explorer100: (limited availability) AKTA Explorer 100 system is equipped with 2mm UV flow cell, 2mL mixer, FR-902 & FR-904, and on-line filter. UNICORN software is version 5.31 with strategy ECAF411. 7.1 Method of Sample Loading: A2 A11 A18 S1 S8 B1 or B2 P-960 (not recommended by Wuxi AppTec’s AKTA Maintenance Program) Super Loop (not supplied by WuXi AppTec) 8.0 AKTA Avant150 Preferred number of Avant150: Sample Loop (not supplied by WuXi AppTec) (limited availability) AKTA Avant 150 systems equipped with 2mmUV flow cell, 5 mL mixer, FR-902, and on-line filter. UNICORN software is 6.3 with strategy MDH090. 8.1 Method of Sample Loading: S A B Super Loop (not supplied by WuXi AppTec) 9.0 Sample Loop (not supplied by WuXi AppTec) Indicate which, if any, of the following activities will be performed at WuXi AppTec during this study: Column Packing Column Evaluation (HETP/Asymmetry) Buffer Prep 10.0 Indicate number of client & filter vendor personnel who will be visiting WuXi AppTec for this study: 11.0 Preferred visit dates to WuXi AppTec: WuXi AppTec is prepared to provide only the aforementioned materials and equipment (within availability limits). Any other equipment needs should be discussed with your Project Manager. Name of person completing this form Email Address for Contact Company Name VC-ERF-032615