See Instructions in EMV´s HMS-page
Risk Assessment of
Department
Group
Date

Tasks included in the risk assessment

Included chemicals, mixtures and solutions
Chemical, mixture or solution
CAS Number
Amount
Indication of danger (hazard statement and limit value)
Ethanol 99,5%
64-17-5
800 ml
H225 Highly flammable liquid and vapour
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
500
Ethanol 95%
64-17-5
700 ml
1000
1000
1900
H225 Highly flammable liquid and vapour
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
500
Ethanol 80%
64-17-5
200 ml
1000
1000
1900
H225 Highly flammable liquid and vapour
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
500
Hydrochloric acid, conc 37%
7647-01-0
5 ml
1000
1000
1900
H314 Causes severe skin burns and eye damage.
H335 May cause respiratory irritation.
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
Chemical, mixture or solution
CAS Number
Amount
Indication of danger (hazard statement and limit value)
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
5
Xylene
1330-20-7
600 ml
8
H226 Flammable liquid and vapour.
H312 Harmful in contact with skin.
H315 Causes skin irritation.
H332 Harmful if inhaled.
Caution! Skin penetrating substance according to The Swedish Work Environment Authority’s
provisions and on occupational exposure limit values, AFS 2011:18
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
50
242 g?
221
100
442
Tris(hydroxymetyl)aminometan (s)
77-86-1
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H335 May cause respiratory irritation.
SDS (s)
151-21-3
H228 Flammable solid.
H302 Harmful if swallowed.
H311 Toxic in contact with skin.
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H335 May cause respiratory irritation.
Isobutyl alcohol
78-83-1
H226 Flammable liquid and vapour.
H315 Causes skin irritation.
H318 Causes serious eye damage.
H335 May cause respiratory irritation.
H336 May cause drowsiness or dizziness.
NB! Skin penetrating substance according to The Swedish Work Environment Authority's provisions
and on occupational exposure limit values, AFS 2011:18
Level limit value
Ceiling limit
value
Short-term value
Total
dust
Respirable
dust
[ppm] [mg/m3] [ppm] [mg/m3] [ppm] [mg/m3] [mg/m3] [mg/m3]
50
150
75
250
Chemical, mixture or solution
CAS Number
Amount
Indication of danger (hazard statement and limit value)
Identification of risks of accident or ill-health, assessment of risks and suggestion of preventive measures
Task
Identified risk/problem
Assessment of risk, see risk matrix
Consequence
Run the gel (electrophoresis)
Probability
Preventive measures
Assessed
Risk
Potential for exposure via
inhalation and direct contact
with eyes and skin.
Potential fire hazard.
Use labcoat, safety goggles and disposable nitrile gloves.
Work in fume hood or on ventilated bench with Plexiglas
hood. Write in handling and safety instruction.
Potential for exposure via
inhalation and direct contact
with eyes and skin.
Potential fire hazard.
Use labcoat, safety goggles and disposable nitrile gloves.
Change gloves frequently, especially if you suspect
contamination. Remove gloves carefully without touching
skin.
Work in fume hood. Write in handling and safety instruction.
The limit values for hydrochloric acid and ethanol are not
exceeded at any occasion.
All staff participate in mandatory fire training every 5 years..
Labs are equipped with fire extinguishers.
No risk associated with task.
Use labcoat, safety goggles and disposable nitrile gloves.
Potential for serious electrical
shock or electrocution due to
leaking chamber, faulty or
corroded electrode cables, or
faulty power supply.
Potential for exposure via
inhalation and direct contact
with eyes and skin
Potential fire hazard
Mycket
allvarliga
Möjligt
Mycket
riskfyllt
Ensure all equipment is in good working order, and that lid is
always on electrophoresis tank when in operation.
Write in handling and safety instruction.
Estimated
risk after
preventive
measure
Acetic acid
Potential for exposure to
highly corrosive vapours, and
for direct contact of corrosive
solution with eyes and skin
Clean and take care of waste
Potential for exposure via
inhalation and direct contact
with eyes and skin
Potential fire hazard.
Potential environmental risk.
Transport and handle
chemicals and bottles
Drop bottles or chemicals
potential risk for exposure via
inhalation or direct contact
with eyes and skin.
Potential fire hazard.
Allvarliga
Möjligt
Riskfyllt
Store acids and bases below waist height. Transport on trolley
or in a bucket. Make sure that you are prepared in case of a
spillage. Write in handling and safety instruction
Centralt placerade spillkit samt tillhörande instruktion tas
fram, se EMV:s HMS-handlingsplan för 2013.
In case of power or
ventilation failure or if
evacuation is necessary
Fumes evaporate to lab
Moderate
Low
High risk
Close containers and close fume hood. Write in handling and
safety instruction.
At power failure or
evacuation
Fumes evaporate to lab
Cleaners
If glass ware not rinsed before
sending to be washed,
potential for exposure via
direct contact with eyes and
skin.
Same personal protective equipment as during the task
Dispose of as chemical waste.
Close containers and close fume hood. Write in handling and
safety instruction.
Serious
Possible
Moderate
risk
Before sending glass ware to be washed it must be properly
rinsed. Write in in handling and safety instruction.
Cleaning must only be performed in free areas and cleaners
must not wipe spill. Write in instruction for cleaners.
Have hazardous tasks been defined and rules for working alone been considered?
Have risks during power/water failure etc or because of evacuation been considered?
Do you have preparedness in case of spillage, eg first aid, absorption material, gloves?
Do you have a written handling and safety instruction for this work?
Do you have a written instruction for cleaners and other maintenance personal?
References

Protocol for
Task in the Improvement plan for EMV 2013
Ej riskfyllt



SDS for all chemicals
LU´s Handling Regulations for Management of hazardous waste
EMV:s HMS-webpage
Participants
Reviewed by
Approved by Research Group Leader
Name in printing