HPHE 6720 L ABORATORY T ECHNIQUES IN E XERCISE S CIENCE
Topic 3 – Determination of Lactate and Ventilatory Thresholds
Laboratory Write-Up Assignment (Fall 2013)
Determination of Lactate Threshold
You will be provided with data for this section.
Using the data provided:
1.
Determine the lactate threshold using the two-line regression model a.
Include the printout of the graph that you produce i.
VO
2
(LO
2
∙min -1 ) vs. Lactate Concentrations (with trendlines/equations) b.
Provide the following data for the LT: i.
VO
2
(LO
2
∙min -1 ) at LT ii.
% VO
2 max at LT
2.
Determine the following for the LT cut-off values of 2.0 mmol∙L -1 and 4.0 mmol∙L -1 : a.
Include the printout of the graph you produce i.
VO
2
(LO
2
∙min -1 ) vs. Lactate Concentration (with polynomial trendline/equation) b.
Provide the following data: i.
VO
2
(LO
2
∙min -1 ) at 2.0 and 4.0 mmol∙L -1 ii.
% VO
2 max at 2.0 and 4.0 mmol∙L -1
Determination of Ventilatory Threshold
You will be provided with data for this section.
Using the data provided:
1.
Determine the VT using the “Ventilation” graph a.
Include the printout of the graph you produce i.
VO
2
(LO
2
∙min -1 ) vs. Ventilation (L∙min -1 ) (with trendlines/equations) b.
Provide the following data: i.
VO
2
(LO
2
∙min -1 ) at VT ii.
%VO
2max
at VT
2.
Determine the VT using the “V-Slope” method a.
Include the printout of the graph you produce i.
VO
2
(LO
2
∙min -1 ) vs. VCO
2
(L∙min -1 ) (with trendlines/equations)

b.
Provide the following data: i.
VO
2
(LO
2
∙min -1 ) at VT ii.
%VO
2max
at VT
3.
Determine the VT using the “Ventilatory Equivalents” method a.
Include the printout of the graph you produce i.
Workload (Watts) vs. VE/VO
2
and VE/VCO
2 b.
Provide the following data: i.
Workload at VT ii.
VO
2
(LO
2
∙min -1 ) at VT iii.
%VO
2max
at VT
Interpretation Questions
1. How well did our values for lactate/ventilatory threshold agree between the different methods? For example, what was the % difference for the VO
2
at LT/VT between:
A. LT using two-line regression model vs. VT using VO
2
vs. VE method
B. LT using two-line regression model vs. VT using VO
2
vs. VCO
2
(V-Slope) method
C. LT using two-line regression model vs. VT using Ventilatory Equivalents method
Remember: % difference = ((VO
2
for Method 2 – VO
2
for Method 1) / VO
2
for Method 1) x 100
2. Find a research paper that examines the relationship/correlation between lactate threshold and ventilatory threshold and report what relationship they found (i.e. how closely did the two methods agree?). Provide the citation and a copy of the abstract of the article.
What to Turn-In
1.
A copy of your Excel Worksheet with all of the data (so I can see what points you included in the graphs and your calculations of VE/VO2 and VE/VCO2.
2.
The LT-Two Line Regression graph with the requested calculations.
3.
The LT-Cut-Off Values graph with the requested calculations.
4.
The VT-Ventilation graph with the requested calculations.
5.
The VT-V-Slope graph with the requested calculations.
6.
The VT-Ventilatory Equivalents graph with the requested calculations.
7.
Your response to Interpretation Question #1
8.
Your response to Interpretation Question #2 with abstract attached.