GMO Lab Equipment
Lesson 1
Common workstation 95°C water bath
 Microcentrifuge
 Balance and Weigh boats
 Mung beans (certified non GMO food) – take from freezer the night before
the lab and soak in water – easier to break up in class.
Student Workstations (8) –
 2 screwcap tubes w/ 500μl InstaGene Matrix
 Styrofoam cup (dH2O in class)
 10ml graduated cylinder
 Transfer pipet (sterile – they come with kit)
 Mortar & pestile (glass) – remind instructors to grind the non GMO food
samples first to avoid cross contamination with GMO samples.
 Test foods (students bring to class)
 Marking pen
Lesson 2
Common workstation –
 Thermal cycle
 Bucket of ice
Student Workstations –
 PCR tubes
 PCR adapters
 Foam microtube holder
 2/20ul Pipeter and tips
 Instruction Cards
 One tube of GMO+ (50ul) - see prep notes below
 One tube of PMM (70ul) - see prep notes below
 One tube of GMM (70ul) - see prep notes below
Lesson 2 Advanced prep
 Thaw the GMO+ DNA template and pulse spin the tubes in a centrifuge.
Add 50μl of GMO positive DNA template to 8 screwcap tubes labeled
GMO+. This can be prepared ahead of time and stored at -20°.
 One hour prior to lab thaw the master mix and primers and pulse-spin.
Keep tubes on ice.
 Label 18 screwcaps as follows:
o 9 – “PMM”
o 9 – “GMM”
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Add 600μl of master mix to one PMM & one GMM tube
Add 12μl of green primers to the master mix in the PMM tube and mix.
Store on ice.
Add 12μl of red primer to the master mix in the GMM tube. Store on ice.
Add 70μl of the plant master mix with the green primers to each of the
remaining 8 PMM tubes.
Add 70μl of the GMO master mix with the red primers to each of the
remaining 8 GMM tubes.
Day 2
Lesson 3
Common workstation –
 Destaining Tray
 3% Agarose in 0.25x TAE in 60°C water bath (5x 150ml Erlenmeyer
flasks) Make 600ml in 1L Erlenmeyer on hot plate and aliquot.
 Recycle Flask for Fast Blast and one for Buffer
 3L 0.25x TAE
Student workstations –
 Samples from lesson 2
 One tube each of PCR molecular ruler ‘MWR’ and orange loading
buffer ‘LD’ (see prep notes below)
 2-20 pipet w/ tips
 Gel box kit
 Power supply
 Fast Blast 100x Stain
 Staining Tray
 Tape
 Instruction Cards
Lesson 3 Advance prep:
 Thaw the orange G loading dye and PCR molecular weight ruler and pulse spin
tubes to bring all contents to bottom.
 Label 8 tubes LD and 8 tubes MWR
 Add 70 ul of orange G to each of 8 tube marked LD. This can be done ahead of
time and stored at 4oC for 1-2months.
 Add 25ul of molecular weight ruler to each of tubes marked MWR. This can be
done ahead of time and stored at 4oC for 1-2 months.
 0.25X TAE Buffer – to make 3L of 0.25X TAE – add 15ml of 50X TAE to 2.985L
of dH2O
 100X Fast Blast – to make 1L of 100X Fast Blast add 200ml of 500X Fast Blast
to 800ml DH2O
Note: Wash mortar and pestles in bleach between each lab section to avoid
cross contamination.