Table S1: List of localities sampled and their geographic coordinates (UTM). The last column shows the sampling years for each locality, old and current respectively. 1Source: Montagud & Arrizabalaga, 1980. 2Source: Arrizabalaga et al., 1986 & review for A. flavicollis in Fernández, 2012. 3Source: Culí et al., 1989. 4Source: unpublished data from Museu de Ciències Naturals de Granollers. 5Source: Requejo, 2011. Altitude Locality UTM (1×1) Marata 31TDG4311 250 Llerona 31TDG4111 237 Arbúcies 31TDG5929 300 Centelles 31TDG3527 525 Tona 31TDG3533 600 El Brull 31TDG4229 840 La Vola 31TDG4759 800 L’Ajuda 31TDG3530 587 (m) Place Parish church of Santa Coloma de Marata Parish church of Santa Maria de Llerona Parish church of Sant Quirze and Santa Julieta Parish church of Santa Coloma de Centelles Town Samples (old-current) Marata 19771 – 2014 Llerona 19781 – 2014 Arbúcies 19832 – Centelles 19832 – Church of Sant Andreu de Tona Tona 19832 – Church of Sant Martí del Brull El Brull 19832 – Clergy house of Sant Andreu de la Vola (Cal Sastre) Sanctuary of Mare de Déu de l’Ajuda Sant Pere de Torelló Hostalets de Balenyà 19863–20115 19914 – 2014 Table S2: Relative frequency and differences in frequencies (%) of the small mammal species in the barn owl pellets in the old (1977–1991) and current (2011–2014) samples. Species with significant changes were marked (+P < 0.07; *P < 0.05; ** P < 0.01; ***P < 0.001), after log-linear model fitting to the two way contingency tables generated by the factors small mammal species x period (old/current). Data from Apodemus spp. where analysed together since no accurate differentiation between A. sylvaticus and A. flavicollis was provided in the old sample at the locality of La Vola. Species Old samples (N and %) Current samples (N and %) Frequency difference * P < 0.05 Sorex minutus 25 0.58 4 0.22 −0.36+ Sorex araneus 12 0.28 0 0.00 −0.28** Crocidura russula 1,247 29.16 452 25.06 −4.10** Suncus etruscus 68 1.59 27 1.50 −0.09 Eliomys quercinus 3 0.07 0 0.00 −0.07 Myodes glareolus 4 0.09 0 0.00 −0.09 Arvicola sapidus 5 0.12 0 0.00 −0.12 Microtus agrestis 37 0.87 9 0.50 −0.37 Microtus duodecimcostatus 555 12.98 120 6.65 −6.32*** Apodemus flavicollis 8 0.19 27 1.50 1.31 Apodemus sylvaticus 994 23.24 522 28.94 5.70 1,012 23.66 549 30.43 6.77*** 1,185 27.71 469 26.00 Apodemus spp. Mus spretus −1.71 Mus musculus 90 2.10 96 5.32 3.22*** Rattus norvegicus 2 0.05 32 1.77 1.73*** Rattus rattus 42 0.98 46 2.55 1.57*** Total 4,277 1,804 Table S3. Absolute (number of individuals) and relative frequencies (in brackets) of small mammal species found in barn owl pellets in the four localities with old (1977–1991) and current (2011–2014) samples available. Species Sorex minutus Sorex araneus Crocidura russula Suncus etruscus Eliomys quercinus Myodes glareolus Arvicola sapidus Microtus agrestis M.duodecimcostatus Apodemus flavicollis Apodemus sylvaticus Mus spretus Mus musculus Rattus rattus Rattus norvegicus Total Marata Old Current 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 410 (32.62) 243 (28.79) 23 (1.83) 12 (1.42) 0 (0.00) 0 (0.00) 1 (0.08) 0 (0.00) 5 (0.40) 0 (0.00) 0 (0.00) 0 (0.00) 20 (1.59) 52 (6.16) 0 (0.00) 2 (0.24) 222 (17.66) 232 (27.49) 495 (39.38) 188 (22.27) 56 (4.46) 63 (7.46) 25 (1.99) 25 (2.96) 0 (0.00) 27 (3.20) 1257 844 Llerona Old Current 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 323 (39.53) 84 (15.64) 15 (1.84) 14 (2.61) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 16 (1.96) 10 (1.86) 0 (0.00) 0 (0.00) 106 (12.97) 158 (29.42) 335 (41.00) 221 (41.15) 5 (0.61) 24 (4.47) 17 (2.08) 21 (3.91) 0 (0.00) 5 (0.93) 817 537 L’Ajuda Old Current 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 352 (24.70) 53 (22.84) 28 (1.96) 1 (0.43) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 0 (0.00) 479 (33.61) 33 (14.22) 8 (0.56) 4 (1.72) 215 (15.09) 75 (32.33) 314 (22.04) 57 (24.57) 27 (1.89) 9 (3.88) 0 (0.00) 0 (0.00) 2 (0.14) 0 (0.00) 1425 232 La Vola Old Current 25 (3.21) 4 (2.09) 12 (1.54) 0 (0.00) 162 (20.82) 72 (37.70) 2 (0.26) 0 (0.00) 3 (0.39) 0 (0.00) 3 (0.39) 0 (0.00) 0 (0.00) 0 (0.00) 37 (4.76) 9 (4.71) 40 (5.14) 25 (13.09) 41 (5.27) 2 (0.26) 0 (0.00) 0 (0.00) 778 3 (1.57) 0 (0.00) 0 (0.00) 0 (0.00) 191