Kingdom of Saudi Arabia
‫اململكة العربية السعودية‬
Ministry of Higher Education
‫وزارة التعليم العايل‬
Salman Bin Abdul-Aziz University
‫جامعة سلمان بن عبد العزيز‬
College of Applied Medical
‫كلية العلوم الطبية التطبيقية‬
Sciences
Midterm1 Examination 1
Course Title: Gene and molecular technology
Course Code: MLAB 475
Year: 1433/1434
Year: 4th
Level: seventh
Time allowed: 1 hour
Date: 21-10-1433
Number of papers: 7
Number of questions: 4
Student Name : …………………………………………………………………………………………
Student Number: ……………………………………………………………………………………..
Question no
QI
QII
QIII
QIV
Total
Question
Scores
Multiple choice
Complete
True and fales
Bounance
Student
Scores
5
18
5
2
30
Prepared by: Ms. Ghannimh ALghuwainem
Signature:
Group A
ANSWER ALL THE FOLLOWING QUESTIONS
18
Part 1: choose the correct answer
1. Which statement is NOT correspond with the direction of 5’ to 3’
a. Forming of Okazaki Fragments
b. Leading strand
c. Building up of mRNA
d. The DNA ligase action
2. Stop site is
a. Where transcription is stopped
b. UAG codon
c. AUG codon
d. A and B
e. A and C
3. Splicing means
a. Introns are removed from DNA strand
b. Introns are removed from tRNA strand
c. Exons are removed from mRNA strand
d. Introns are removed from mRNA strand
4. Building up of mRNA is accomplished by
a. Ligase
b. DNA plymerase I
c. DNA polymerase III
d. RNA polymerase
5. Building up of RNA primer is by
a. RNA primase
b. Single strand binding proteins
c. Helicase
d. RNA polymerase
6. The AUG triplet codes for
a. Methionin
b. Stop codon
c. Start codon
d. A and C
7. The nucleus is the place where ................................ take place
a. Replication
b. phase of protein synthesis
c. Translation
d. A and B
e. All of the above
8. The sugar founded in DNA result of transcription is
a. Ribose with lack of ( - OH )
b. Ribose
c. Glucose
d. Fructose
9. What is pictured next in the DNA
replication process?
a. Replication fork
b. Replication bubble
c. Leading strand
d. Lagging strand
10.
During tanscription, the mRNA
a. Totally identical non–template DNA strand
b. Complementary to non–template DNA strand with exception
c. Complementary to template DNA strand with exception
d. None of the above
11. mRNA can be found in
a. In nucleus only
b. In cytoplasm
c. In nucleus and with ribosomes
d. None of the above
12.
Which strand grows continuously towards the replication
fork?
a. Lagging strand
b. DNA strand
c. leading strand
d. All of the above
13.
a.
b.
c.
d.
Introns can NOT be found in
Pre- mRNA
DNA
Functional RNA
Noe of the above
a.
b.
c.
d.
The triplet sequence TAC on DNA codes for
Stop codon
Methionine
Start codon
B and C
a.
b.
c.
d.
What are the four nitrogen bases for DNA?
Uracil, thymine, Adenine and Guanine
cytosine, thymine, Adenine and Guanine
Lytosine, thymine, Adenine and Guanine
Cytosol, thymine, Adenine and Guanine
14.
15.
16.
a.
b.
c.
d.
17.
a.
b.
c.
d.
18.
a.
b.
c.
d.
e.
Which of complementary pairs are NOT correct
T—A
G—C
A—G
U—A
Which of the following is NOT found in RNA
Ribose
Uracil
Phosphate
Thymine
Which of the following statement is correct
C pairs with G by 3 hydrogen bounds
G pairs with C by 2 hydrogen bounds
G pairs with A by 3 hydrogen bounds
C pairs with G by 5 hydrogen bounds
C pairs with G by peptide bounds
Part 2: complete the sentences
1. Regarding transcription, .................................is a sequence of
nucleotides which is made up by coping one strand of DNA
sequence which is called.........................strand. This DNA strand
cannot immigrate from....................... to ..................where the
protein is synthesised.
2. Protein is made of sequence of ordered ..............................which
have been linked together by .................... bounds
3. during DNA replication process,......................... sequence is
essential before calling for DNA polymerase III to add
deoxyribonucleotides .
4. Replication process occurs in....................during ................ of cell
division
5. Okozaki fragments are a part of newly synthesized DNA strand
called............................ which will be connected by ....................
enzyme
6. The reasons which make mRNA as an advantage
is......................................................................................................
and .................................................................................................
7. Two components of gene structure are................................and
......................................
8. The region where transcription starts is called ...............................
9. The result of DNA replication is..............................................
10.
The enzyme which is responsible unwinding of double strand
of DNA helix is called ......................... in replication and
..............................in transcription
11.
The codon UUG codes for........................, however the
amino acid VALINE is coded by ......................or ............................
12.
Examples of Pyrimidines;........................... and....................
13.
Protein synthesis consists of two parts:
………………………… and………………….……
12
Part 3 : True and False
1. (
) One gene codes for one protein
2. (
) One amino acid is coded by only one codon
3. (
) In transcription, RNA primer is important to initiate synthesis
of RNA
4. (
) In DNA structure, nucleotides are attached to phosphate
group
5. (
) One codon codes for more than one amino acid
6. (
) RNA polymerase is an enzyme which can add
deoxyribonucleotides
7. (
) Splicing happens after synthesis of primary RNA transcript
8. (
) The backbone of the DNA molecule is composed of
alternating phosphate groups and sugars linked by strong covalent
bonds)
8
Part 4 : boununce question
1.
According to DNA replication, which enzyme is
responsible for replacing RNA primer to DNA
nucleotides?
2