Iain Fraser Cytometry Centre (IFCC)
Dr. Raif Yuecel (raif.yuecel@abdn.ac.uk), Linda Duncan (l.duncan@abdn.ac.uk), Attila Bebes (a.bebes@abdn.ac.uk)
+44 (0)1224 437597 (Room 2.26) or - 437595 (Lab 2.54)
USER REGISTRATION FORM
All users of the Cytometry Centre are required to register by completing the form.
Items marked with a * must be completed.
First Name*
Middle Initial
Last Name*
School*
Principal Investigator*
Division/Section*
Room number*
Office/Lab Phone*
E-mail address*
Are you a UoA member of staff or student?*
No
Yes
Please provide more details on your position (e.g. PI, technician, honour, MSc, PhD student, Postdoc, etc.)
If NO, please complete also the following part 1) – 3):
1) Organization* (e.g. University, Company, NHS, etc)
3)
2)
Department/Division/Section*
Billing Address/Postcode*
Project Title*
Project/Grant Code to be charged*:
Describe the Project
USER REGISTRATION FORM
Page 1 of 5
Iain Fraser Cytometry Centre (IFCC)
Dr. Raif Yuecel (raif.yuecel@abdn.ac.uk), Linda Duncan (l.duncan@abdn.ac.uk), Attila Bebes (a.bebes@abdn.ac.uk)
+44 (0)1224 437597 (Room 2.26) or - 437595 (Lab 2.54)
Sample type (e.g. mouse leukocytes, human PBMC, human cellline U937, bacteria, yeast etc.)*
Are the samples*
Fresh
Fixed?
Staining reagents you intend to use* (e.g. Propidium iodide, CFSE, FITC, PE etc)
Do the samples contain any hazardous or infectious agents?*
Yes
No
If YES, list them and indicate the hazards/has the infectious agent been inactivated.
List any known human pathogen/were the cells transformed using a virus?
Are the samples considered to be Genetically Manipulated Organism (GMO)?*
Yes
No
If Yes, do you have the necessary approval from the GM Committee?
Yes
No
In case of GMOs, please be aware of the Local Rules for the Cytometry Work with GMOs at the end of
this registration form.
Are the samples from a human donor?*
Yes
No
Were the blood cells screened for blood borne pathogens?
Have the cells been tested for mycoplasma infection?
Yes
Yes
No
No
If No, you may stop at this point, sign on the next page and return the form to the facility.
If YES, continue on the next page.
USER REGISTRATION FORM
Page 2 of 5
Iain Fraser Cytometry Centre (IFCC)
Dr. Raif Yuecel (raif.yuecel@abdn.ac.uk), Linda Duncan (l.duncan@abdn.ac.uk), Attila Bebes (a.bebes@abdn.ac.uk)
+44 (0)1224 437597 (Room 2.26) or - 437595 (Lab 2.54)
Are samples screened for infection material?
(e.g. with hepatitis B or C or human immunodeficiency virus (HBV, HCV, and HIV)?
Yes
No
If NO, you cannot proceed with Flow Cytometry experiments unless local rules are followed. All freshlyisolated human cells not screened for exposure to the above infectious agents, should be fixed prior to analysis
and local Risk Assessment procedures followed.
For questions contact
Prof. Janet Liversidge (j.liversidge@abdn.ac.uk) at 01224-437504.
NOTE: It is expected that any changes in the types of experiments, cell types, and other
information, pertinent to the safe use of the facility, has to be discussed with and communicated
to the Head of Cytometry centre, prior to undertaking these new studies.
PRINCIPAL INVESTIGATOR:
Please indicate by your signature that you authorise:
1) The above named member of your lab to use the Cytometry Centre
2) The Cytometry centre to bill the above code for any charges.
3) I have read the questions carefully and certify the information provided to be correct
Signature of Principal Investigator (required)
Date
USER:
Please indicate by your signature that you agree to Standard Operating Procedures, Guidelines and Policies of the
facility.
Failure to do so may result in prehibiting your further use of our instrumentation and services, and may also
result in additional charges for misuse.
Signature of User
Date
Please return to Flow Cytometry Core Staff Member (see head of the form)!
Submit
Appendix:
Reset
Local Rules for the Cytometry Work with GMOs
USER REGISTRATION FORM
Page 3 of 5
Iain Fraser Cytometry Centre (IFCC)
Dr. Raif Yuecel (raif.yuecel@abdn.ac.uk), Linda Duncan (l.duncan@abdn.ac.uk), Attila Bebes (a.bebes@abdn.ac.uk)
+44 (0)1224 437597 (Room 2.26) or - 437595 (Lab 2.54)
Cytometry work with genetically modified micro-organisms (GMO)
Local Rules (generic) Activity Class 1
Supervisors: Professor Janet Liversidge & Dr. Raif Yuecel
Activity Class 1: present negligible risk to humans or the environment
Culture volumes: - up to 100ml
These rules apply to the work under this project in labs 2.54 using standard incubator and fridge (GMO cell
staining, centrifugation, analysis) or 1.64 (GMO cell sorting and/or analysis).
1.
These rules are displayed prominently in the laboratory at all times but display the ‘GM work in progress.
See local rules’ sign above your workspace when this work is in progress.
2.
Prepare fresh hypochlorite or Virkon solution each day as disinfectant. (Chloros 100,00 ppm, 1 in 10
dilution, Virkon 1%)
3.
Contaminated tips must be ejected into a beaker containing hypochlorite 100,000 ppm and submerged as
well as possible. Pastettes must also be put into this beaker. Allow to soak overnight. Pour off chloros
down sink. Transfer beaker contents to a CinBin. When CinBin is full or the end of the current set of
experiments is reached or after one week, whichever is the soonest, seal the CinBin and put out for
disposal by incineration.
4.
Liquid waste, such as FACS rinse and FACS buffer must be added to chloros to give a 1:10 chloros
dilution and left overnight before discarding down the sink.
5.
All other waste including tubes, gloves, wipes must be placed in double autoclave bags and autoclaved
(4.07) at 121°C for 30 minutes when bag is full or when the end of the current set of experiments is
reached or after one week, whichever is the soonest. This is the responsibility of the person doing these
experiments. Do not leave this for someone else to do.
6.
Use 70% ethanol or 1% Virkon as appropriate for wiping smaller areas of spill. Use hypochlorite, 100,000
ppm, for larger areas.
7.
For transfer of tubes containing CMO between labs use a sealed container with the lid firmly fixed.
8.
Should tubes need to be stored overnight before analysis this should be within a labelled sealed box in the
fridge in Room 2.27.
9.
All cytometers are routinely cleaned internally using 1:200 chloros and are sterilised using 70% ethanol.
External surfaces and work surfaces are cleaned with 70% alcohol. A log will be taken of all nonUniversity of Aberdeen projects undertaken.
USER REGISTRATION FORM
Page 4 of 5
Iain Fraser Cytometry Centre (IFCC)
Dr. Raif Yuecel (raif.yuecel@abdn.ac.uk), Linda Duncan (l.duncan@abdn.ac.uk), Attila Bebes (a.bebes@abdn.ac.uk)
+44 (0)1224 437597 (Room 2.26) or - 437595 (Lab 2.54)
Local Rules (generic) Activity Class 2
Activity Class 2: presents a risk to humans or the environment
Culture volumes: - up to 100ml
These rules apply to the work under this project in labs 2.54 using standard incubator and fridge (GMO cell
staining, centrifugation, analysis) or 1.64 (GMO cell sorting and/or analysis).
1.
These rules are displayed prominently in the laboratory at all times but display the’GM work in progress.
See local rules’ sign above your workspace when this work is in progress.
2.
Client to confirm CU2 approval obtained and notified and to advise of any specific hazards and safety
measures required prior to FACS work commencing.
3.
Prepare fresh hypochlorite or Virkon solution each day as disinfectant. (Chloros 100,00 ppm, 1 in 10
dilution, Virkon 1%)
4.
Sample preparation and handling to be carried out in Class II safety cabinet in Lab 4.09.
5.
Centrifugation to be carried out in capped tubes within sealed centrifuge buckets and opened in safety
cabinet to minimise aerosols.
6.
No glass or sharps to be used. Contaminated tips must be ejected into a beaker containing hypochlorite
100,000 ppm and submerged as well as possible. Pastettes must also be put into this beaker. Allow to
soak overnight. Pour off chloros down sink. Transfer beaker contents to a CinBin. At the end of the
experiment seal the CinBin and put out for disposal by incineration.
7. Liquid waste, such as FACS rinse and FACS buffer must be added to chloros to give 1:10 chloros dilution
and left overnight before discarding down the sink.
8.
All other waste including tubes, gloves, wipes must be placed in double autoclave bags, blue tagged and
autoclaved (4.07) at 121°C for 30 minutes when bag is full or at the end of the experiment. This is the
responsibility of the person doing these experiments. Do not leave this for someone else to do.
9.
Use 70% ethanol or 1% Virkon as appropriate for wiping smaller areas of spill. Use hypochlorite, 100,000
ppm for larger areas.
10. For transfer of tubes containing CMO between labs use a sealed contained with the lid firmly fixed.
11. Should the tubes need to be stored overnight before analysis this should be within a labelled sealed box in
the fridge in Room 2.27.
12. All cytometers are routinely cleaned internally using 1:200 chloros and are sterilised using 70% ethanol.
External surfaces and work surfaces are cleaned with 70% alcohol. A log will be taken of all nonUniversity of Aberdeen projects undertaken.
USER REGISTRATION FORM
Page 5 of 5