SUPPLEMENTARY INFORMATION Soil development and nutrient availability along a two million year coastal dune chronosequence under species-rich Mediterranean shrubland in southwestern Australia Benjamin L. Turner and Etienne Laliberté This file contains descriptions of soil profiles along the Jurien Bay chronosequence, Western Australia. There is a description of a profile pit on each of six chronosequence stages ranging in age from < 100 years to > two million years. The soils were classified according to US Soil Taxonomy. Profile: Quindalup Young Dune Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: Diagnostic features: ENTISOL: Carbonatic, thermic, Typic Xeropsamments Close to plot QY18 50J 0308318 6654770 17 August 2012; late wet season 19 m above sea level Side of a large dune; ~20% slope facing south Xeric Thermic Quindalup sand; young phase Sparse low stature shrubby vegetation Extremely well drained Mostly bare sand None observed None Throughout the profile, but few > 88 cm Color values are for moist soil and textures are field textures (i) Ochric epipedon (ii) Xeric moisture regime (iii) > 40% carbonate in the mineralogy control section Horizon description AC—0 to 12 cm; light brownish grey (2.5Y 6/3; 10YR 7/2 dry) calcareous sand; loose, single grain; many extremely fine sand binding roots; common medium and few fine roots; few clean quartz grains among calcareous fragments; smooth gradual boundary. C1—12 to 88 cm; light grey (2.5Y 7/2; 10YR 8/1 dry) calcareous sand; loose, single grain; moist; few fine patches of organic matter (decaying roots); few coarse, medium, fine, and very fine roots; smooth gradual boundary. C2—88+ cm; white (2.5Y 8/1; 10YR 8.5/1 dry) calcareous sand; loose, single grain; dry; very few fine and medium roots; non-cohesive (collapsing after excavation). Profile: Quindalup Medium Dune Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: Diagnostic horizons and features: ENTISOL: Carbonatic, thermic, Typic Xeropsamments Road cut near plot QM 26, along the dune to the south 50J 0308536 6655422 18 August 2012; late wet season 11 m above sea level Backslope of dune; ~10% facing southeast Xeric Thermic Quindalup sand; medium phase Low stature shrubby vegetation < 1 m high; relatively dense at ground level Extremely well drained Some grass and moss on surface None observed None Throughout the profile to 116 cm Color values are for moist soil and textures are field textures (i) Ochric epipedon (ii) Xeric moisture regime (iii) >40% carbonate in the mineralogy control section Horizon description A—0 to 13 cm; very dark grayish brown (2.5Y 3/2; 10YR 5/1 dry) loamy sand; loose, single grain; moist, but dry at surface; many very fine, and common coarse, medium, and fine roots; smooth clear boundary. CA—13 to 51 cm; grayish brown (2.5Y 5/2; 10YR 6/1 or 6/1 dry) calcareous sand; loose, single grain; moist; few coarse, and common medium, fine, and very fine roots; smooth gradual boundary. C1—51 to 116 cm; pale brown (2.5Y 7/2; 10YR 7/2 dry) calcareous sand; loose, single grain; few fine and medium roots; smooth gradual boundary. C2—116+ cm; pale brown (2.5Y 8/2; no change dry) calcareous sand; loose, single grain; moist; no roots; noncohesive, collapsing from exposed faces. Profile: Quindalup Old Dune Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: ENTISOL: Siliceous, thermic, Typic Xeropsamments Road cut near plot QO11 50J 0313077 6658353 13 August 2012; late wet season 20 m above sea level Crest of a dune Xeric Thermic Quindalup sand; old phase Shrubby vegetation < 1 m high Well drained Bare soils and dry leaves (<10% cover) None observed None Throughout the profile Color values are for moist soil and textures are field textures. The B horizon is not cambic because although there is evidence of decalcification and a higher color value than the underlying C horizon, the texture is not very fine sand or finer. Diagnostic horizons and features: (i) Ochric epipedon (ii) Xeric moisture regime Horizon description A—0 to 12 cm; dark greyish brown (10YR 4/2; 2.5Y 5/2 dry) loamy sand; very weak blocky structure; very friable; common coarse, medium, fine, and very fine roots; smooth clear boundary. BA—12 to 26 cm; yellowish brown (10YR 5/4; 10YR 6/2 dry) sand; very weak blocky structure; very friable; common coarse, medium, fine, and very fine roots; smooth gradual boundary. B1—26 to 60 cm; light yellowish brown (10YR 6/4; 10YR 7/3 dry) sand; loose, single grain; few very fine, fine, and medium roots; smooth diffuse boundary. B2—60 to 93 cm; pale brown (2.5Y 7/4; 10YR 8/3 dry) sand; loose, single grain; few very fine, fine, and medium roots; smooth diffuse boundary. B3—93 to 165+ cm; pale brown (2.5Y 7/4; 10YR 8/2 dry) sand; (slightly lighter than above); loose, single grain; few very fine, fine, and medium roots. Profile: Spearwood Young Dune Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: INCEPTISOL: Sandy, siliceous, thermic, Calcic Haploxerepts Jurien Bay East Road; north of road 50J 0314984 6648707 18 August 2012; late wet season 31 m above sea level Crest of a shallow dune Xeric Thermic Spearwood sand; Tamala Limestone pinnacles within 1 m of the soil surface Shrubby vegetation < 1 m high Well drained Bare soil (~25%) and dry leaves None observed None Throughout the profile Color values are for moist soil and textures are field textures. The profile qualifies as an Inceptisol because of the petrocalcic horizon, and as Haploxerepts because although the petrocalcic horizon is within 150 cm of the surface, the soil does not have free carbonates in all layers above this. It is a Calcic Haploxerepts because of the sandy particle-size class and secondary carbonates within 150 cm of the mineral soil surface. Diagnostic horizons and features: (i) Ochric epipedon (ii) Xeric moisture regime (ii) > 90% resistant material in the sand fraction in the particle control section (iv) Petrocalcic horizon with upper boundary between 40 and 100 cm below the soil surface Horizon description A—0 to 11 cm; dark grayish brown (10YR 4/2; no change dry) loamy sand; weak coarse blocky structure; very friable; many very fine, and common medium and fine roots; smooth clear boundary. AE—11 to 18 cm; brown (10YR 5/3; no change dry) sand; very weak medium blocky structure; very friable; common medium, fine, and very fine roots; smooth clear boundary. B1—18 to 33 cm; brownish yellow (10YR 6/6; 10YR 5/6 dry) sand; loose, single grain; common very fine, and few fine and medium roots; smooth gradual boundary. B2—33 to 100+ cm; brownish yellow (10YR 6/7; 10YR 6/6 dry) sand; loose, single grain; few very fine, fine, and medium roots; limestone pinnacles reaching to 40 cm, but sand extending to > 1 m on profile face. Bkkm—petrocalcic horizon (calcrete) of undetermined thickness, over limestone. Profile: Spearwood Old Dune Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: Diagnostic horizons and features: ENTISOL: Thermic, Xeric Quartzipsamments Jurien Bay East Road, south of the road 50J 0321438 6651482 17 August 2012; late wet season 60 m above sea level Crest of a shallow dune; ~5-10% slope Xeric Thermic Spearwood sand Shrubby vegetation < 1 m high, with a few tall Banksia Well drained Bare soil (~50%) and dry leaves None observed None Throughout the profile Color values are for moist soil and textures are field textures (i) Ochric epipedon (ii) Xeric moisture regime (iii) > 90% resistant material in the sand fraction in the particle control section Horizon description A—0 to 11 cm; dark grayish brown (10YR 4/2; 10YR 5/2 dry) loamy sand; weak coarse blocky structure; very friable; thin crust of organic matter and roots on surface; many very fine, and common coarse, medium and fine roots; smooth clear boundary. AE—11 to 25 cm; pale brown (10YR 6/3; no change dry) sand; weak coarse blocky structure; very friable; many very fine, and common coarse, medium and fine roots; few hollow old root casings with hard exteriors; smooth clear boundary. E—25 to 38 cm; light yellowish brown (10YR 6/4; 10YR 7/4 dry) sand; loose, single grain; many very fine, and common coarse, medium and fine roots; smooth gradual boundary. BE—38 to 55 cm; brownish yellow (10YR 6/6; 10YR 7/5 dry) sand; loose, single grain; few coarse, medium, fine, and very fine roots; smooth gradual boundary. B—55 to 140+ cm; brownish yellow (10YR 6/7; 10YR 7/6 dry) sand; loose, single grain; few coarse, medium, fine, and very fine roots. Profile: Bassendean Profile Classification: Profile location: UTM: Date and season: Elevation: Slope and site position: Soil moisture regime: Soil temperature regime: Parent material: Vegetation: Drainage: Surface features: Faunal activity: Coarse fragments: Rooting depth: Notes: Diagnostic horizons and features: ENTISOL: Thermic, Xeric Quartzipsamments Close to plot BL4 50J 0318055 6659016 13 August 2012; late wet season 67 m above sea level Flat Xeric Thermic Bassendean sand Banksias ~2-3 m high over shrubby vegetation Well drained (water table at 3.2 m) Shallow layer of dry leaves (~75%) None observed None Throughout the profile to >165 cm Color values are for moist soil and textures are field textures (i) Ochric epipedon (ii) Xeric moisture regime (iii) >90% quartz in the particle control section Horizon description O—0 to 3 cm; very dark brown (10YR 2/2) organic-rich, root rich, mat-like horizon; many very fine, fine, medium and coarse roots, including many clusters; few quartz grains; smooth abrupt boundary. A—3 to 30 cm; very dark grey (2.5Y 3/1; 2.5 or 10YR 5/1 dry) loamy sand; weak blocky structure; very friable; common medium and coarse, and many very fine and fine roots; smooth gradual boundary. E1—30 to 70 cm; light grey (2.5Y 7/1; no change dry) sand; single grain; common coarse and very fine, and few medium and fine roots; smooth diffuse boundary. E2—70 to 320 cm; white (2.5Y 8/1; 2.5Y 9/1 dry) sand; single grain; few medium and coarse, and very few fine and very fine roots; smooth diffuse boundary. B—320+ cm; very pale brown (10YR 7/3 dry) sand; saturated (water table at 320 cm).