Title: Efficacy of Management Tools for Armored Scale in Containers Protocol #: 15-006 Draft p 1 of 4 Objective: Determine efficacy of various products against armored scale on ornamental plants in containers. Do not conduct this protocol with in-ground plants. Experimental Design: Plot Size: Must be adequate to reflect actual use conditions. Replicates: Minimum of 6 replications. Target Species: Camellia, Euonymus, Japanese Maple, and Tea scales. Contact your regional coordinator if other target species are of interest. Plant Hosts: Use a plant host suitable for target scale species, recording species and variety used. Record crop species and variety used. Treatments: See treatment table on next page. Test materials should have been stored for no more than 2 years prior to start of experiment. Biological test materials should be requested within 2 weeks prior to first application unless otherwise directed by manufacturer. Application Instructions: Apply each treatment according to directions in the table below. All treatments are foliar applications. Applications should be made using application equipment consistent with conventional commercial equipment. Calibrate application equipment prior to application. Read available labels before making applications. Avoid treating plants under unusually extreme environmental conditions. Use Site: May be greenhouse or field container. For field in-ground, refer to protocol 14-007. Evaluations: Record initial insect counts and then 7, 14 (prior to 2nd application), 28 days, and six months after initial application or at an appropriate time point to assess efficacy. Record phytotoxicity at each rating date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material. Maintenance Applications: Applications during an experiment to manage non target diseases, pests or weeds may be needed to appropriately evaluate efficacy or crop safety. Because of potential negative impacts due to simultaneous or sequential applications of certain products with test materials please avoid applying the following: If applications must be made of any products on the above list, please contact IR-4 prior to application. Recordkeeping: All operations, data and observations appropriate to this study should be recorded. It is helpful to review the Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Other information to record includes soil-type or soil-less media, application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth stage at application and data collection dates. Content of reports should be sufficient to fully understand how the experiment was conducted. Photographs often illustrate experimental design, site conditions, sampling methods, and impacts of treatments very well. It is encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are visually apparent. If different application methods or evaluations are made, please clearly specify differences in final report and explain reason for change. Treatments: Priority # Product 1 IKI-3106 (cyclaniliprole) 22 fl oz per 100 gal 2 IKI-3106 (cyclaniliprole) 28 fl oz per 100 gal Mainspring 200SC (cyantraniliprole) 4 A/N 5 NoFly (Paecilomyces fumosoroseus strain FE 9901) 28 oz per 100 gal 6 Distance (pyriproxifen) 12 fl oz per 100 gal 7 Talus 70DF (buprofezin) 14 oz/100 gal 20 gm/1 gal container 40 gm/2gal container 60gm/3 gal container Flagship G (thiamethoxam) 8 0.5 g product/ ft of shrub height Kontos (spirotetramat) 3.4 oz per 100 gal 3.4 oz per 100 gal Broadcast evenly around the surface of the growing media Apply in enough drench volume to wet the upper ½ to ¾ of the root zone. Foliar Drench Draft Flagship 25WG (thiamethoxam) Two foliar sprays on a 21 day interval. Apply at crawler stage. Foliar application p 2 of 4 B (Choose one) 8 fl oz per 100 gal (2.4 ml per gal) Title: Efficacy of Management Tools for Armored Scale in Container A 12 fl oz per 100 gal (3.6 ml per gal) Application Instructions Full coverage spray at crawler stage and every 2 weeks for a maximum of 3 applications. Full coverage spray at crawler stage and every 2 weeks for a maximum of 3 applications. Apply as a single drench application. Apply 30 days prior to infestation if test system allows. Drench application volume of final solution: 4” pot – 2 fl oz 5” pot – 3 fl oz 6” pot – 4 fl oz 7”pot – 5 fl oz 8” pot – 6 fl oz For larger pots – 8 fl oz per gal of growing media Drench volume applied should not leach through bottom of pot. Apply as two drench applications at a 28 day interval. Apply the initial application 30 days prior to infestation if test system allows. Drench application volume of final solution should be based on pot size as in the above treatment. Drench volume applied should not leach through bottom of pot. Full coverage spray at crawler stage and every 2 weeks for a maximum of 3 applications. Protocol #: 15-006 3 Rate Safari 20SG (dinotefuran) Container: 18 oz/ 100 gal TriStar 30SG (acetamiprid) 12 oz per 100 gal 9a 9b Standards (Pick One) 9c 9d 10 Tank Mix: Distance + Tristar Orthene TTO Horticultural Oil Acephate E 75 WP (75% acephate) Chlorpyrifos E-Pro 2 (2 lb ai/gal chlorpyrifos) Untreated 0.67 lbs/100 gal 2 qts/100 gal -- Make applications at crawler stage on a 14 day schedule. Make no more than 2 consecutive applications. Apply at crawler stage. Full coverage spray at crawler stage and every 4 weeks for a maximum of 3 applications. Full coverage spray at crawler stage or early stages of settled scales. -- Title: Efficacy of Management Tools for Armored Scale in Container C 2.75 oz per 100 gal+ Capsil at 6 oz per 100 gal 3.5 oz per 100 gal+ Capsil at 6 oz per 100 gal 12 fl oz + 12 fl oz per 100 gal See label directions See label directions Protocol #: 15-006 Xxpire 40WG (spinetoram + sulfoxaflor) Single soil drench: 4 fl oz of drench solution/ gallon of potting media. Apply evenly to soil surface. Spray to wet; two foliar sprays on a 14 d interval with a wetting agent such as Capsil. Draft p 3 of 4 Title: Efficacy of Management Tools for Armored Scale in Containers Protocol #:15-006 Draft p 4 of 4 Sources for Products: Product Distance Flagship 25WG Flagship G GF-2860 40WG Kontos Mainspring NoFly Safari 20SG Talus 70DF TriStar 30SG Contact NuFarm, Jill Calabro Syngenta, Steve Cosky Syngenta, Steve Cosky Dow AgroSciences, Dan Loughner OHP, Carlos Bogran Syngenta, Steve Cosky Natural Industries, George Stallings NuFarm, Jill Calabro SePRO, Rich Dirks NuFarm, Rick Fletcher Phone 443-235-0612 336-632-7148 336-632-7148 215-630-6288 979-255-7687 336-632-7148 208-841-9850 443-235-0612 317-216-8253 856-296-0422 Email Jill.Calabro@us.nufarm.com steve.cosky@syngenta.com steve.cosky@syngenta.com dloughner@dow.com cbogran@ohp.com steve.cosky@syngenta.com georges@naturalindustries.com Jill.Calabro@us.nufarm.com RichardD@sepro.com rick.fletcher@us.nufarm.com Reports: Reports are due 60 days after last data collection date. Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be submitted as picture embedded in the report or as separate jpg, bmp, or tiff files. Please direct questions to: Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540, Phone 732-932-9575 x4629, palmer@aesop.rutgers.edu OR Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: 410-923-4880, E-mail: evvea@comcast.net. Draft Date: 10/28/2014 Revised By: EV & CLP