MOLECULAR ECOLOGY RESOURCES (2012) 12: 570-572
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Isolation of microsatellite markers from red drum,
Sciaenops ocellatus, and characterization in red
drum and spotted seatrout, Cynoscion nebulosus
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MARK A. RENSHAW, CHRISTOPHER M. HOLLENBECK, and JOHN R. GOLD
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A total of 323 nuclear-encoded microsatellites were isolated from an enriched genomic
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DNA library of red drum, Sciaenops ocellatus, and characterized in 20 individuals each
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of both red drum and spotted seatrout, Cynoscion nebulosus. The microsatellites include
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240 perfect repeats (224 dinucleotide, nine trinucleotide, four tetranucleotide, and three
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pentanucleotide) and 83 imperfect repeats. For red drum, 301 microsatellites were
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polymorphic; six deviated significantly from Hardy-Weinberg expectations following
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Bonferroni correction. For spotted seatrout, 172 microsatellites were polymorphic and
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only one deviated significantly from Hardy-Weinberg expectations following Bonferroni
Center for Biosystematics and Biodiversity, Department of Wildlife and Fisheries Sciences,
Texas A and M University, College Station, TX 77843-2258, USA
Correspondence: Mark A. Renshaw, Fax: (979) 845-4096; E-mail: mrenshaw@ag.tamu.edu
Keywords: microsatellites, Sciaenops ocellatus, Cynoscion nebulosus, red drum, spotted
seatrout
Abstract
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correction. The microsatellite markers characterized in this study will be useful for
39
future aquaculture and population-genetic research in both species.
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The red drum, Sciaenops ocellatus, is an estuarine-dependent fish distributed in the
42
western Atlantic Ocean from Massachusetts in the northeastern United States (U.S.) through
43
the Gulf of Mexico to Tuxpan, Mexico. Red drum is an important aquacultured species in the
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U.S. and elsewhere, both to supplement ‘wild’ fish with hatchery-spawned fingerlings
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(Tringali et al. 2008; Vega et al. 2011) and to produce a viable commercial product (Lutz
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1999; http://www.fao.org/fishery/culturedspecies/Sciaenops_ocellatus/en#tcNA0078). The
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related species Cynoscion nebulosus (spotted seatrout) is also aquacultured for stock
48
enhancement along the U.S. Gulf Coast (Vega et al. 2011; http://www.usm.edu/gcrl/
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research/seatrout_main.php). Stock-enhancement programs benefit from the use of genetic
50
tools, such as microsatellites, through assessment of genetic diversity (Perez-Enriquez et al.
51
1999) and unequivocal identification of hatchery-spawned fish in the ‘wild’ as a means to
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assess stock-enhancement success (Karlsson et al. 2008a).
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Here, we report development of 323 nuclear-encoded microsatellites from an enriched
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genomic library of red drum and their characterization in samples of red drum and spotted
55
seatrout. Previously, Renshaw et al. (2009) demonstrated a reasonable success rate in cross
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amplifying microsatellites in spotted seatrout based on polymerase-chain-reaction (PCR)
57
primers developed for red drum. The rationale for the work was to generate microsatellites
58
that could be used to further saturate the genetic map for red drum generated recently by
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Portnoy et al. (2010) and to initiate a genetic map for spotted seatrout. Genetic maps for both
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species can be used as a tool to enhance selective breeding through identification of
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quantitative trait loci and marker-assisted selection (Liu & Cordes 2004) as well as use in
62
association genetic studies (Ewens & Spielman 2001).
3
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Generation of an enriched genomic library of red drum DNA followed protocols outlined
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in Renshaw et al. (2010). DNA fragments were hybridized at 58o C for 1.25 hours with
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50pmol of a 3’-biotin modified (CA)13 oligonucleotide, bound to streptavidin-coated magnetic
66
beads (Dynabeads M-280,Invitrogen), and then put through a series of washes to remove
67
fragments without targeted microsatellite motifs. Microsatellite-enriched DNA fragments
68
were ligated into the pCR 2.1-TOPO vector (Invitrogen) and transformed into Escherichia
69
coli (One Shot TOP10 Chemically Competent Cells, Invitrogen). A total of 864 positive
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(white) clones were sent to the University of Florida’s Interdisciplinary Center for
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Biotechnology Research (http://www.biotech.ufl.edu/) for sequencing with the M13 forward
72
primer. Sequences were edited and vectors trimmed using SEQUENCHER 4.1 (Gene Codes).
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In addition, eight red drum microsatellite-containing sequences available on GenBank were
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evaluated (denoted as Scoc in Table 1). Primer pairs were developed using PRIMER3
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(http://frodo.wi.mit.edu/). An additional 27 microsatellite markers (Soc744, Soc753, Soc758,
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Soc759, Soc761, Soc762, Soc770, Soc773, Soc778, Soc781, Soc783, Soc785, Soc786, Soc792,
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Soc796, Soc800, Soc804, Soc807, Soc810, Soc812, Soc814, Soc819, Soc825, Soc826, Soc834,
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Soc835, and Soc837), previously described in Portnoy et al. (2011), were included to assess
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population statistics for both red drum and spotted seatrout.
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Fin clips were taken from 20 red drum and 20 spotted seatrout sampled near Corpus
81
Christi (Texas). DNA was extracted using a modified Chelex protocol (Estoup et al. 1996).
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PCR amplifications followed the protocol outlined in Karlsson et al. (2008b), using 1µl of
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DNA, 1x Colorless GoTaq Flexi Buffer (Promega), 2mM MgCl2, 200µm of each dNTP,
84
5pmol of a fluorescently labeled 21bp 5’-tail-sequence primer (5’-
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GCCTCGTTTATCAGATGTGGA-3’), 5pmol of a reverse sequence primer, 0.5pmol of a
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third oligonucleotide consisting of the 5’-tail-sequence followed by the forward primer
87
sequence, and 0.5U of GoTaq Flexi DNA Polymerase (Promega). The 5’-tail-sequence
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primer was labeled with one fluorescent label of Dye Set D (Applied Biosystems): 6-FAM,
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HEX, or NED. Amplified PCR products were run on an ABI 377 automated sequencer.
90
Alleles were sized using the Genescan 400HD [Rox] Size Standard (Applied Biosystems);
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allele sizing and calling were performed using GENESCAN 3.1.2 and GENOTYPER version 2.5
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software. Genetic variability of each microsatellite was evaluated as number of alleles, gene
93
diversity (expected heterozygosity), and observed heterozygosity. Wright’s FIS, estimated as
94
Weir Cockerham’s f and using GDA (Lewis & Zaykin 2001), was used to measure departure
95
of genotype proportions from Hardy-Weinberg expectations at each microsatellite. MICRO-
96
CHECKER
97
alleles and scoring errors due to stuttering and/or large allele dropout.
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(Van Oosterhout et al. 2004) was used to assess evidence of occurrence of null
Of the total of 426 microsatellites assayed, 323 consistently amplified and were
polymorphic in at least one of the two species. Summary data for these microsatellites are
100
presented in Table 1. A total of 301 markers were polymorphic in red drum, with the number
101
of alleles ranging from 2 to 26; expected heterozygosity ranged from 0.0500 to 0.969, while
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observed heterozygosity ranged from 0.0500 to 1.000. Following Bonferroni correction for
103
multiple tests (Rice 1989), genotypes at six microsatellites (Soc750, Soc886, Soc937, Soc960,
104
Soc992, and Soc1003) deviated significantly from Hardy-Weinberg expectations; analysis
105
with MICRO-CHECKER indicated the possibility of null alleles at 36 markers (Table 1), with
5
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seven of these (Soc797, Soc886, Soc892, Soc944, Soc945, Soc1003, and Soc1136) exhibiting
107
evidence of scoring errors due to stuttering.
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A total of 172 microsatellites were polymorphic in spotted seatrout (Table 1), with the
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number of alleles ranging from 2 to 27; expected heterozygosity ranged from 0.0500 to 0.969,
110
while observed heterozygosity ranged from 0.0500 to 1.000. Following Bonferroni correction
111
for multiple tests (Rice 1989), genotypes at only one microsatellite (Soc1061) deviated
112
significantly from Hardy-Weinberg expectations; analysis with MICRO-CHECKER indicated the
113
possibility of null alleles at 14 microsatellites (Table 1), with three of these (Soc847, Soc955,
114
and Soc1061) exhibiting evidence of scoring errors due to stuttering. The microsatellites
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characterized in this study will be useful for future aquaculture and population genetic
116
research in both red drum and spotted seatrout.
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Acknowledgements
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We thank C. Caster and A. Sulak for technical assistance in the laboratory and R. Vega and R.
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Chavez for procuring samples. Funding was provided by the Saltonstall-Kennedy Program
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(Award NA10NMF4270199) of the National Marine Fisheries Service (U.S. Department of
123
Commerce) and by Texas AgriLife Research (Project H-6703). This paper is No. 84 in the
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series ‘Genetic Studies in Marine Fishes’ and Contribution No. XXX of the Center for
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Biosystematics and Biodiversity at Texas A & M University.
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References
129
Estoup A, Larigiader CR, Perrot E, Chourrout D (1996) Rapid one tube DNA extraction for
130
reliable PCR detection of fish polymorphic markers and transgenes. Molecular Marine
131
Biology and Biotechnology, 5, 295-298.
132
133
134
Ewens WJ, Spielman RS (2001) Overview: locating genes by linkage and association.
Theoretical Population Biology, 60, 135-139.
Karlsson S, Saillant E, Bumguardner BW, Vega RR, Gold JR (2008a) Genetic identification of
135
hatchery-released red drum in Texas bays and estuaries. North American Journal of
136
Fisheries Management, 28, 1294-1304.
137
138
Karlsson S, Renshaw MA, Rexroad CE III, Gold JR (2008b) PCR primers for 100 microsatellites
in red drum (Sciaenops ocellatus). Molecular Ecology Resources, 8, 393-398.
139
Lewis PO, Zaykin D (2001) Genetic Data Analysis (GDA): Computer Program for the Analysis
140
of Allelic Data, Version 1.0 (d16c). Free program distributed by the authors via the internet
141
from http://hydrodictyon.eeb.uconn.edu/people/plewis/software.php.
142
143
Liu ZJ, Cordes JF (2004) DNA marker technologies and their applications in aquaculture
genetics. Aquaculture, 238, 1-37.
144
Lutz CG (1999) Red drum: a reemerging aquaculture species. Aquaculture Magazine, 25, 35-38.
145
Perez-Enriquez R, Takagi M, Taniguchi N (1999) Genetic variability and pedigree tracing of a
146
hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement, based
147
on microsatellite DNA markers. Aquaculture, 173, 431-423.
148
149
150
151
152
153
154
Portnoy DS, Renshaw MA, Hollenbeck CM, Gold JR (2010) A genetic linkage map of red drum,
Sciaenops ocellatus. Animal Genetics, 41, 630-641.
Portnoy DS, Hollenbeck CM, Renshaw MA, Gold JR (2011) Microsatellite panels for gene
localization in red drum, Sciaenops ocellatus. Aquaculture, 319, 505-508.
Renshaw MA, Gawriluk TR, Gold JR (2009) Characterization of red drum microsatellite
markers in spotted seatrout. North American Journal of Aquaculture, 71, 374-379.
Renshaw MA, Portnoy DS, Gold JR (2010) PCR primers for nuclear-encoded microsatellites of
155
the groupers Cephalopholis fulva (coney) and Epinephelus guttatus (red hind). Conservation
156
Genetics, 11, 1197-1202.
7
157
Rice WR (1989) Analyzing tables of statistical tests. Evolution, 43, 223-225.
158
Tringali MD, Leber KM, Halstead WG, McMichael R, O’Hop J, Winner B, Cody R, Young C,
159
Neidig C, Wolfe H, Forstchen A, Barbieri L (2008) Marine stock enhancement in Florida: a
160
multi-disciplinary, stakeholder-supported, accountability-based approach. Reviews in
161
Fisheries Science, 16, 51-57.
162
Van Oosterhout C, Hutchinson WF, Shipley P (2004) MICRO-CHECKER: software for identifying
163
and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4, 535-
164
538.
165
Vega RR, Neill WH, Gold JR, Ray MS (2011) Enhancement of Texas sciaenids (red drum and
166
spotted seatrout). In: Interactions of fisheries and fishing communities related to
167
aquaculture (R. Stickney, R. Iwamoto, and M. Rust, eds.). Proceedings of the Thirty-Eight
168
U.S.-Japan Aquaculture Panel Symposium, Corpus Christi, Texas, October 26-27, 2009.
169
U.S. Dept. Commerce, NOAA Tech. Memo. NMFS-F/SPO-113, 85-92 p.
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Table 1 – Summary of 323 microsatellite markers isolated from red drum, Sciaenops ocellatus, and
characterized in both red drum (top) and spotted seatrout, Cynoscion nebulosus (bottom).
Msat
Primers
Motif
Soc742
GTGAGGGTCGAACGTTTTGT
AAGTGTCCCACGGATGACTC
GACTCATCCTGGCTCTCTGC
TCTAGGACAGCATGGGCTCT
TGTCTTCAGATGGACGCAGA
CAGAGAGGGCTTGTTTGAGG
TTTGCAAGTGTCCGTCTGTC
TGGTGCATTATGAAAGCCTAC
CCATGTGGCAGATGACAGAC
GGGGCGTCTTAACACACATT
TTGTGGTTATGGTTCCATTCAA
AAAATCTGCGGGATTACAGC
TGTAATGCAGCGATATGGTCA
TGTAATGCAGCGATATGGTCA
CACGTTTTTGTCTTTGCACAC
ACAAACACAAAGCCGAGGAG
TCCAGCCTGCTCAGATTTTT
AAAGCAGGATGCAGTTCACTC
GAGTGAACTGCATCCTGCTTT
TCGCTCTCGCTCTCTCTTTC
TGTGAGCTGCTGGACTGTTC
TGGGACTCGATGTATCCTGA
CCAGGATGCCAAGGATACAA
TGCCTTACACAATGCTGGAG
GCAGAAAAGCCCTGTTTCAA
TGCATGCCAATCTCATCATT
GTCCAATGCAGCAAAGAAAA
TTGACAGCACGGACACATTT
TGCAAACGTTCTGTGAGACC
CTCCCTTGTCTCTGGGATCA
AAGCAGGCTCAGTCTTCAGC
CCCCCAAAATTACCAAATCTC
CCACTGTCTTGACACTGCGT
GCTGCTGTGAGCTCAATAACTC
GTATGACCACATGGGGCTTC
TGGACGCCTGTAGTCTTGTG
TGGACGCCTGTAGTCTTGTG
TGTGAATGCCTAACCAACCA
AGAGCATGGGGGAGTCAGAT
ACAGACACGCAGGAACATAAT
GTTGGGTTTGCCGTAAGAAA
CAATATAACTGTATGTTTGCACTCAC
CCGCTTCCTGCTGAAAATTA
TTCGTGCCTAAACCTAACCAA
CGTTACGTTAGGTCTTCAGTTCA
GCCGCGATAAATGGTATGTT
GATGGGACATCAAGGAGACC
TGAGGTGTGCGAGTTAGACG
ATACACGTAAGCGCACCTGA
ACGGCCAGACATACAAGGAC
AGGGAGACAGCTGCAGAGAA
GGTGCAGAGAGGCAGAAAAC
TCGATCGAGCCACCTAATCT
AGCGAGCGCTAATATCGTGT
CAGCACTAGAAGCCACACGA
TCACCTCTCAGATCTGTTTCCA
ATTCCCTGCTCACATCCAAC
TCCTTCACTGGACACACCAA
CCTGACAATAGACACAAACACATACA
Soc743
Soc744
Soc745
Soc746
Soc747
Soc750
Soc751
Soc753
Soc754
Soc755
Soc758
Soc759
Soc760
Soc761
Soc762
Soc766
Soc767
Soc768
Soc770
Soc772
Soc773
Soc774
Soc777
Soc778
Soc779
Soc781
Soc782
Soc783
Soc785
GenBank
N/NA
Size Range
(GGT)5
Clone
Size
85
JQ235209
(GT)117bp(TG)5
142
JQ235210
(AC)53bp(CA)11
236
JF509110
(CT)52bp(CT)5
149
JQ235211
(AC)11
279
JQ235212
(GT)5
110
JQ235213
(AC)27
149
JQ235214
(GT)6
93
JQ235215
(AC)26
155
JF509111
(TG)8
115
JQ235216
(GT)8
95
JQ235217
(AC)62bp(AC)5
12bp(AC)16
(GT)20
289
JF509112
210
JF509113
(TG)7(AG)8
274
JQ235218
(AC)7
205
JF509114
(AC)9
286
JF509115
(CTG)5
184
JQ235219
(AC)8
215
JQ235220
(CA)6
141
JQ235221
(GT)8
142
JF509116
(GT)9
262
JQ235222
(TC)21
291
JF509117
(CA)8
119
JQ235223
(CT)7(CA)12
183
JQ235224
(TG)19
222
JF509118
(TG)7
239
JQ235225
(GT)11
164
JF509119
(TGA)6
239
JQ235226
(CT)24
106
JF509120
(CA)17
281
JF509121
20/2
20/4
20/13
20/14
20/17
na
20/4
20/6
20/4
na
20/1
20/5
20/10
na
20/3
20/4
na
20/3
na
20/2
20/6
20/4
20/19
20/2
20/12
na
20/5
na
20/4
na
20/6
na
20/2
na
20/4
na
20/4
na
20/3
20/1
20/3
20/1
20/18
20/6
20/1
20/2
20/4
20/3
19/10
15/1
20/2
20/1
20/9
20/5
20/4
na
20/15
20/4
20/13
101-107
107-122
157-183
171-221
254-308
na
169-175
181-209
299-305
na
132
128-138
141-169
na
113-117
115-125
na
134-142
na
129-131
117-131
121-129
307-387
251-253
223-249
na
295-315
na
223-229
na
303-315
na
205-208
na
229-235
na
158-164
na
163-169
145
280-286
231
281-337
267-283
139
139-147
197-207
188-196
225-249
229
262-264
261
183-201
175-187
258-267
na
110-152
116-126
280-334
9
0.1
0.6
0.8
0.8
0.9
0.6
0.6
0.5
0.0
0.4
0.8
0.2
0.6
0.5
0.2
0.5
0.6
0.9
0.0
0.9
0.4
0.5
0.8
0.2
0.2
0.4
0.5
0.0
0.1
0.0
0.9
0.8
0.0
0.0
0.2
0.1
0.8
0.0
0.5
0.0
0.7
0.6
0.6
0.9
0.5
0.9
Soc786
Soc787
Soc790
Soc791
Soc792
Soc793
Soc796
Soc797
Soc798
Soc799
Soc800
Soc802
Soc803
Soc804
Soc805
Soc806
Soc807
Soc808
Soc809
Soc810
Soc812
Soc813
Soc814
Soc815
Soc818
Soc819
Soc820
Soc821
Soc822
Soc823
Soc825
Soc826
CCTTCACTGCAGGTGCTACA
TCTCTCCCACTTTTATTCTCTTTCTC
GGAGTGAGAAAGTCCCACGA
CCATCTCCCTGACACAGGAT
CAGGGCTGGAAGTAGTTGCT
GCCTGTGCAACTGCTAAGTG
CATGTTAACCAGTCGCAGTGA
GCCTCTCTAACAGGGCACAC
AGGGATCTGCTCCTGATGAA
GCACCATAACCTCCCATCAC
GCCCCGCTTTAAATAACCAT
TGCGCACATGACACAGACTA
CTGGAGCTGCCCACATTTAT
GTTGAGGAGGTCATCGTCGT
TCACCCTCCTGTCCACTCAT
TTTACAGCAGAAATGGTGTGC
GCCACATGACAATGAAGCAA
CCTTGGCTTTCATTTCTCCA
TTTGGTCTTTTTCACCTTCCA
CACCACCCACCCCTTACTTA
GCCTAGGAGGAGAGCCACTT
AGTTGGTGTGGCTGTTCTGA
TGCACCACTGACAGACAAAAG
TGCGTTTGTGACTGTGAGTG
GTGTGTGCTGTCTCCCACTG
GGGGAGCACTGAGACAAAAA
CCATGAAACACTCTGAAGTGC
GTGCTCGATCTCTCCGTCTC
CCGTTGTCTGGTCCCTCTAA
CATGGAGAGGGAGGAATGAA
CCAAAGGAAGCAGGCAAGTA
GCTATTTGCCAACCCTCAAA
TTTAGCGGCCTTAAGCAAGA
ACCATTCCTCCGGATCATAA
GCTGTGCCCAGATTTTCACT
GTCGCTAGGACAGAGGATGC
CAGCTCACAAAGGTGGACAG
ATCCTGTGAAACGCTCCAAT
TGTACGAGGGGCTAAAATGC
AACACGCACTTGCTCTCTCA
AATAAATCGGGGGAAACTGG
AAGGCATCACTTCCAACATTT
CATGGAGACATCACCGTTTG
TCAGGTCAGGTTTGTGTCCA
TTTTCCTATTTAAGGTTTGCTCTC
CCTCCCCATAATTGTGCCTA
TGATATGTGGGGAACTGTGTG
CAAAAGGCAAAGCAAGAGGA
TGTCAGCAGCTACAGGGAAA
GCCTGATCATCACGTCTGTC
GTGGACCAGAGCTCCCAGTA
CATGTGATCCGCTCAATGAC
CGTGTCACCTGTGGAAACTG
GCCGGCGTTACATCTCATTA
AGTCGAAGCCAGCAAAACAT
CACATGAGTCCCCACTCTCA
AGCCTGTCGACCAATGATGT
AGCACGACCAGCCAATTTAC
ATTGGGGCAAACAGAGACAG
GGTTGTGCAGCCGGTATAGT
GCGCTCTCCTGTTTCATCTC
CATGCAACATTAGCCCAGTG
TGTTGATGAGCAGCCTTACG
GGCAGGATTTAGGCAATTCA
ACACACTCCTGTGTGCAACC
(CT)38
179
JF509122
(CA)53
200
JQ235227
(GT)8
235
JQ235228
(GAG)7
176
JQ235229
(GT)92bp(GT)16
200
JF509123
(CA)61bp(AC)15
199
JQ235230
(CA)17
238
JF509124
(CA)10
248
JQ235231
(TCACA)5
175
JQ235232
(CT)6
192
JQ235233
(TG)15
183
JF509125
(AC)6
271
JQ235234
(AC)9
202
JQ235235
(CT)9(CA)12
248
JF509126
(TTCTA)5
199
JQ235236
(TG)8
177
JQ235237
(CA)14
141
JF509127
(GT)10
113
JQ235238
(GT)52bp(GT)8
177
JQ235239
(CT)6(CA)10
150
JF509128
(TG)27
143
JF509129
(GA)16
163
JQ235240
(AC)13
123
JF509130
(GT)6
171
JQ235241
(CA)9
179
JQ235242
(CA)201bp(AC)5
274
JF509131
(GA)7
173
JQ235243
(AC)9
216
JQ235244
(CA)10
292
JQ235245
(CCAG)5
267
JQ235246
(GT)12
204
JF509132
(GTGA)11
180
JF509133
na
20/15
20/2
na
20/5
20/3
na
20/1
20/5
20/10
20/8
20/4
na
20/14
na
20/3
20/6
20/3
20/2
20/1
20/2
20/8
17/11
20/1
20/2
20/4
na
20/5
20/2
20/3
na
20/3
na
20/8
20/5
20/8
20/6
20/4
na
20/6
na
20/19
na
20/11
na
20/3
na
20/3
20/1
20/6
20/14
20/9
20/19
20/6
20/4
20/4
20/7
20/7
20/2
20/1
20/2
20/9
na
20/6
20/18
na
163-203
154-158
na
126-134
246-260
na
198
185-200
221-255
171-189
218-224
na
243-277
na
268-274
253-271
192-202
192-197
214
205-207
195-213
183-221
292
288-290
222-236
na
260-270
267-269
210-220
na
198-206
na
158-172
161-175
133-149
129-149
186-202
na
170-180
na
133-161*
na
177-209
na
138-142
na
193-199
190
206-228
212-264
294-310
230-310
189-199
180-186
236-244
239-271
307-329
312-314
288
274-278
224-244
na
183-211
210-294
10
0.9
0.0
0.3
0.6
0.0
0.4
0.7
0.8
0.5
0.8
0.3
0.6
0.1
0.2
0.0
0.4
0.8
0.8
0.0
0.0
0.3
0.5
0.1
0.3
0.3
0.7
0.6
0.8
0.7
0.4
0.7
0.9
0.9
0.5
0.3
0.0
0.7
0.9
0.8
0.9
0.7
0.3
0.2
0.8
0.7
0.1
0.0
0.0
0.8
0.7
0.9
Soc828
Soc829
Soc831
Soc832
Soc833
Soc834
Soc835
Soc836
Soc837
Soc838
Soc839
Soc842
Soc843
Soc844
Soc846
Soc847
Soc848
Soc849
Soc850
Soc851
Soc854
Soc855
Soc857
Soc858
Soc859
Soc860
Soc862
Soc865
Soc866
Soc867
Soc868
Soc869
Soc870
GGGGGTGCAGAGACAGAATA
GTCCAGGGAGAGCAAGTCTG
TCACTGCCATCAGCAGAAAC
TGGCTGCAAACGTAACTCTG
TGTGGGACTTAAGGGGAATG
GCGTCTTGAAACAAGCCTTC
GTGAAGAAGGCAGCGATTTC
ATGCACCCATACTCCCAGAG
AGAACTGCTGGGTGCAATGT
TGTCTTCTTCGTGTGCGTCT
TGAGAACAGCTCTGCCTCCT
TCATTCCGTCAATGTTCAGG
CCTGTGCTCATATGAACAAGA
CACACAGAATCTTTCAGGGATG
CTGCTCCATCTTCCACCTTC
GCGGAGATAGGGACAGAAAG
CAGATGAAGGGAGGGAACAA
CACACAAACATGCACAAGCA
TGCCATCTCATCGGAGGTAT
ACCTCGCCAAATTTGTGAAC
GTTCACAAATTTGGCGAGGT
CAGATAGAAGAAAGAATTCAAAACTGG
CCTACCATGGGTTTTGCTGT
TGCTCACATGAGAGTGTGCAT
CAACAAGCAAACGCACAGTT
CTGGGAGACAACACCAGACA
TGCTTGACCTGAATCATTGC
ACGTGGCATATACCCTCCTG
GCACATACACAGGCATACTTGA
AATCATTGCACCCAAATGGT
CTAGCTCGCAGCTGTCTCCT
AACAGAATGAGCCTGCCAGT
CTGGACCATAGCTTTCAGCAC
GAAAAGCTCTGCTGAGGTCAA
CCTCAGCAGAGCTTTTCACA
CCCTCAGCCCAGATCAAATA
CCGGCCAGTGTATTTGATCT
AGAGAGACAGCGAGGGACAG
CCTCGATAGGTCAGTGTGTGT
TTGGCAGAGAGCCAGACTTT
CCCAGAACCGAGAACAAAAA
CCCTCTTCTCCACCCCTTAC
AAAGGTTTTAATGAGGCAGACG
ACCCACATGGAGACAGGAAG
TGCACACTGAGGCTGGATAG
AGCCTGCAGAAGACACCACT
CGCCGCTCTTATTGTTTTGT
CAAACCCCCATTTCAATCAT
TCTTGTTGTGCCCATGTGTT
TGACGGCTTACAGAGGATGA
TGCGCTCAGATGGTTTTATG
ATCTCCAGCCACAGTCCATC
GGAAGAAACCCACAGAGTGC
CTCCCAGACCAGAAGACTGC
TGTCCTTCCCCTTGAAACTG
GACCCTGGTGCTGACAGACT
TTGCGCGTGGTATTTTTAAG
TTCCCCCAAAAGAAATTGTG
CGCTGGTGAAGCCTCATTAT
TACGGCACATGAAACCTTGA
GCGATCGATTCATCACAAGA
CTCCAAAGCACACACCTCAA
CAGCCCGGTAACCTTTTACA
CAAAAGGAAATGCAAGACTGC
CCATGTGGATGTTGTGAAGC
(AG)7
229
JQ235247
(AC)1013bp(CA)27
160
JQ235248
(GT)13
237
JQ235249
(TC)7
131
JQ235250
(CA)13
172
JQ235251
(AC)20
248
JF509134
(TG)23
112
JF509135
(TC)8
143
JQ235252
(TG)14
168
JF509136
(TC)15
198
JQ235253
(CA)9
146
JQ235254
(CA)6
252
JQ235255
(AC)7
225
JQ235256
(AC)6
281
JQ235257
(CA)8
125
JQ235258
(CA)7
102
JQ235259
(AG)6
99
JQ235260
(CA)8
127
JQ235261
(CT)17
199
JQ235262
(GT)52bp(GT)16
81
JQ235263
(CTT)516bp(CTT)5
163
JQ235264
(TG)11
130
JQ235265
(AG)519bp(GT)13
187
JQ235266
(CA)12
184
JQ235267
(GA)6
261
JQ235268
(CA)16
237
JQ235269
(GT)6
142
JQ235270
(GT)11
88
JQ235271
(GT)9
127
JQ235272
(GA)6
140
JQ235273
(AC)10
126
JQ235274
(AC)142bp(AC)25
261
JQ235275
(GT)5
247
JQ235276
20/2
20/3
20/14
20/3
20/9
na
20/4
20/3
20/14
20/8
20/17
na
20/23
20/6
20/2
20/1
20/7
20/1
19/5
18/9
20/1
20/2
20/1
20/2
20/2
20/1
20/5
na
20/2
20/1
20/1
20/5
20/2
20/1
20/3
na
20/11
20/4
20/9
na
20/2
20/1
20/6
20/7
20/8
20/3
19/14
20/9
20/2
20/13
20/10
20/1
20/3
na
20/7
na
20/5
20/2
20/2
20/3
20/4
20/8
20/12
20/1
20/2
251-253
278-284
163-197
111-117
260-279*
na
150-158
146-154
192-228
196-222
253-303
na
124-167*
100-120
164-168
162
182-210
172
213-223
212-230
166
168-170
272
278-280
243-245
227
301-317
na
146-148
145
123
116-126
146-148
145
144-148
na
213-269
223-229
99-135
na
183-186
157
143-159
139-179
197-217
203-207
201-243
201-223
283-287
300-340
248-282
231
153-163
na
110-130
na
145-153
137-139
158-160
162-166
144-152
151-171
255-287
221
266-270
11
0.4
0.0
0.9
0.1
0.8
0.4
0.1
0.8
0.6
0.9
0.9
0.5
0.1
0.0
0.7
0.0
0.7
0.8
0.0
0.0
0.0
0.0
0.2
0.0
0.6
0.1
0.0
0.0
0.7
0.1
0.0
0.5
0.8
0.7
0.7
0.1
0.0
0.6
0.7
0.7
0.3
0.9
0.8
0.0
0.9
0.8
0.0
0.5
0.7
0.5
0.0
0.0
0.4
0.5
0.7
0.9
0.0
0.0
Soc872
Soc875
Soc876
Soc877
Soc878
Soc880
Soc881
Soc882
Soc883
Soc884
Soc885
Soc886
Soc887
Soc888
Soc890
Soc891
Soc892
Soc893
Soc894
Soc895
Soc896
Soc897
Soc899
Soc900
Soc901
Soc902
Soc903
Soc904
Soc905
Soc907
Soc908
Soc909
GCTATGTCTCCGCACTGTGA
CCGAAAACAAAACCGCTACT
TTCAGGTGCTTTCTGCTCCT
ATAACGCAGCTCGGAGAGAA
TTGCTGCATGTAACCCCTCT
ATTGTCAACGCCAGTGTGAA
CAATTGTCCCAAAGACTCGAA
AAAACAGAACGGGCAAAGG
CATTCTGTGGGTGGTTGTTG
CAAGAGTCGAGATCTTTTCTTCG
AGCGCTGATGACAACCTGAT
GTGTCAAGGACCAGCGATTT
CAGGGAGCACACTGGTTTGT
GCGTGTCTGCACGTGTTATG
AGCAGGAGGTCTGGACTCAA
ACCCACTGCAGCCTGTAATA
TGGAGGATTCGTCATCAACA
AGACTTCCGCTTGTTCTCCA
GAAGCGCTAACATGGGTAGG
AGGCTTTCATCTACAGCATGG
CCTGCAGAGCTTTTTCGTTC
GCACTAGCCCTCATGGGTAA
AGCACAAGAAGGCCAAACAG
GCCCTGAGCAAAGAGAAAAA
GGGCACATTGACTTCTGGTT
TGTGGCGGGGATATATCAAT
CGTGTGTATGGTGGTGATGG
GGAGATGGAGTTTTGTCTGTGC
GCCCAGCCTATAGCATCAAC
GCAGTAACCAGTGCAGACCA
CAGTCTGCCGCATTAAGACA
AGAGGCGAAGAGCACAACAT
AGTTCCATGCACTCCCTGAG
ACCCCTCCCTCTTGTCATTT
GTGTCCAAGCTGCCTCTTTC
GAAAGAGGCAGCTTGGACAC
GAAATGCAGGGAGACATGGT
TTTTTCAGCTGCCATCAGG
TGATGAGCACGAACAAAAGC
GGCGATTATGATGTGTGTGC
CCCGGAGCATACTCTCTGTC
CAACCTCTCATCACACACACG
GCATTTAATGTTGTGTCCAACG
TGAACTGGAAAGAAGTGACATCC
GTGAGGGTGGCTTTTTATGC
CTTGATGCAGCCAGTTCTCC
TGAATTTGCTCAAGTGTGTAGTAGC
GGAGATGGTGATTGGTCAGG
GGTCAGTTTCGCTGAGAAGG
GAGGGAAAAAGAGCCAGAGG
TCAGAGCGAGAGACAGATGG
CAGCAAACATGCCTTCAAGC
CCTGATATGACACGATGAGAGC
GCATGTAGAGACAAGCCCAAC
TTCCCATCTTCCCTGCTATC
CAACAGCTGATAAGACCTGAATG
GACAGACGTGGACCATGAAC
AATGTCACTTTCCTGCTTTCG
ACAGTGTGTGGCCAAGAGC
CTCCCCTCCTCACACTATGG
AACCAAGCCTCGTTATTACCC
GAGGGAGCTGTAGCTGAAGG
AGGGTCTAGGCGTGTTCTGC
GGACGGACCTGAAAGTATCG
CATCATTCCTGGCTGTGAGG
(TG)8
103
JQ235277
(GT)33
200
JQ235278
(AC)7
258
JQ235279
(TG)9
222
JQ235280
(TG)18
197
JQ235281
(CT)18(CA)15(TA)6
194
JQ235282
(TG)5
215
JQ235283
(CA)135bp(CA)5
255
JQ235284
(CT)7(CA)9
252
JQ235285
(TG)239bp(GT)5
157
JQ235286
(TG)7
290
JQ235287
(TC)8
186
JQ235288
(CA)8
220
JQ235289
(CA)7
172
JQ235290
(TG)8
195
JQ235291
(GT)23
140
JQ235292
(CT)14
259
JQ235293
(AC)59bp(CA)25
1bp(AC)17
(AC)9
213
JQ235294
280
JQ235295
(AC)101bp(CA)8
145
JQ235296
(AC)9
88
JQ235297
(CA)30
271
JQ235298
(GACA)7
137
JQ235299
(GT)1619bp
(GT)1219bp(GT)5
(TC)14
260
JQ235300
131
JQ235301
(TG)21
180
JQ235302
(CA)16
152
JQ235303
(AC)29
120
JQ235304
(GT)91bp(TG)6
181
JQ235305
(GT)64bp(GA)34
186
JQ235306
(GT)8
285
JQ235307
(AC)18
225
JQ235308
na
20/3
na
20/22
20/27
20/4
20/2
19/5
na
20/9
20/5
20/16
20/11
20/1
20/2
na
20/16
20/7
na
20/12
20/6
20/2
na
20/2
20/3
20/8
20/9
20/2
20/6
20/10
na
20/12
20/7
20/6
18/6
20/20
20/10
20/4
20/2
20/11
20/5
20/3
na
20/22
20/5
20/3
20/1
20/17
20/6
20/11
20/1
20/13
na
20/5
20/2
20/15
20/15
20/6
na
20/23
20/14
20/6
na
20/10
na
na
113-123
na
197-285
207-303
273-281
280-284
239-247
na
201-227
213-221
203-204
195-223
238
236-238
na
175-209
269-281
na
173-203
121-133
312-314
na
207-209
201-211
236-252
245-271
191-193
186-198
209-235
na
145-171
126-144
271-283
277-289
153-231
150-174
298-304
298-300
147-177
159-185
98-110
na
270-346
249-263
153-161
151
226-340
221-241
153-177
140
190-216
na
169-177
156-158
110-154
102-142
204-240
na
194-272
219-273
301-317
na
236-274
na
12
0.5
0.9
0.9
0.5
0.0
0.5
0.8
0.7
0.9
0.8
0.0
0.5
0.9
0.5
0.8
0.6
0.0
0.5
0.2
0.7
0.8
0.3
0.6
0.8
0.9
0.6
0.8
0.7
0.9
0.8
0.4
0.4
0.8
0.3
0.3
0.9
0.6
0.3
0.0
0.9
0.8
0.8
0.0
0.8
0.7
0.1
0.9
0.8
0.7
0.9
0.9
0.7
0.7
Soc910
Soc911
Soc913
Soc914
Soc915
Soc916
Soc917
Soc918
Soc919
Soc920
Soc921
Soc922
Soc923
Soc924
Soc925
Soc926
Soc927
Soc929
Soc930
Soc931
Soc932
Soc933
Soc935
Soc936
Soc937
Soc940
Soc941
Soc942
Soc943
Soc944
Soc945
Soc946
Soc947
GCCATGGTTAGTCTTTGTGG
TTCCCTCTCCTCTCCTCTCC
AAACAAGTTGGCGAACAAGC
TGAGAGTGCAAAAGCAGAGG
GCACAAAGGACTACTCACACG
CTGGAGACTACACTGGGATGG
GTGGCAGCATGTGAGTAACG
TTCCAACCCCGATTCTAGC
AAGAAACATTTGAGCACTTTTCC
CCAGCCTGTCATTCTGTGC
GCGGAGAGGAATGAAAAGC
ACGAGTCGGTCACTGTTTCC
TTTGTGCTGTCCTGGTATGC
TTTTGAAAGTCAATGATGTGTCC
GAGAGTGATAGAAAGAGTGACAGAGC
TTAGAATTAACTCAGACTTTTGAGAGC
TGAATGAGCATGAATGAGTGC
GCTCTGATCCTCTGACACTGG
CAGAGTTTTGAGTTTCGACAGG
CAGCTCTGCAGGTCTGAGG
CACGTGTGTCCTTTCTGTCC
CAGCAACTGGATGACTTCTGG
AAATCAGACACGTGGTGAAGG
CCTTTTGTCCCACCCTTAGC
GCTAAGGGTGGGACAAAAGG
AGACACTCTGCGGGAGAAGC
TGCTAACATGCACTCCTTGC
GTCAGAGTACCTCTTGTGAATAACC
TAATAAATGGGCTGCAAAGC
CCGCGAAGAGATATTTCAGG
CTGGTTCCTGCTTCACAACC
GCCTTTATTGATGCCTGTGG
ATGGAGAAAACCCTGTGTGG
CAATGTGGTTCTGGTTCTGG
ATTGCCACAGGAATTTGTCC
ACAGCACGTCAGTCAACAGG
GAGATAGAACGCTTCCTCTTGC
TTCCCGGTGATTAGTTGTGG
CCTGAGTCCTGGAGACATCC
GCACTGAGGAAATAGAAAGATGC
TCTGCACCGGTGTCTCAATA
GACAGACAGCTCCTGTTAGCAA
CCATACAAGGCCACTCTTTCC
TTCCAAACATTAGCCAACAGC
CCTGACAAGTCCCACTGTCC
AAGTGTTATCTCATCAGGGATGC
ACAAATGCTTGTGTGCAACC
TTCTCTGTGGGATCCTTTGG
AACTGCTCAGTTGTTCATCAGG
CCTGCTGGTTCTTTTGATGG
GCAGATCAGGAGTTGAGTGG
CTGGAAGTTGGCTGATGACC
TTCCAGGAAAACCACACTCC
TCTACCAAAACAAGCTCACTGC
AATACTCGCGCACATACAGG
GTGTGCATGCATTCTAGACG
TGCTCTTCGGTGACCTCATAC
TCAGCCATCATCCATACTGC
CAAGCTGCTGCATTGATTTAAC
TTTGGAAGCTGAATATCTTTGG
TCATGATCATTTACACTGTGCTGC
TTTGATGGTGGTGTCGAATG
AGCCCCTTTGTTCACATTTC
CTGCCAGGGGAAGTAGGTG
ATGAGACGATTCGCCAGATG
(CA)9
201
JQ235309
(CA)18
253
JQ235310
(CA)12
170
JQ235311
(TG)55bp(GA)5
196
JQ235312
(GT)12
300
JQ235313
(GT)10
237
JQ235314
(AC)10
246
JQ235315
(TG)36
191
JQ235316
(GA)112bp(GA)17
251
JQ235317
(AC)24
279
JQ235318
(AC)24
214
JQ235319
(TG)103bp(GA)8
175
JQ235320
(GT)1341bp(AC)6
156
JQ235321
(AC)16
235
JQ235322
(TG)8
287
JQ235323
(AC)52bp(AC)12
104
JQ235324
(AC)20
166
JQ235325
(CA)7
297
JQ235326
(AC)7
92
JQ235327
(AC)11
158
JQ235328
(ATG)10
219
JQ235329
(AC)115bp(CA)5
148
JQ235330
(TG)29(AG)6
262
JQ235331
(TG)527bp(GT)9
142
JQ235332
(AC)34
232
JQ235333
(CCT)14
180
JQ235334
(CA)19
238
JQ235335
(CA)627bp(CA)5
255
JQ235336
(GT)11
241
JQ235337
(GT)6
242
JQ235338
(AC)9
152
JQ235339
(CA)51bp(AC)5
234
JQ235340
(CA)7
175
JQ235341
20/4
na
20/9
20/2
20/4
20/1
20/1
20/15
20/3
na
20/11
20/1
20/5
16/3
20/17
20/2
20/19
20/7
20/14
20/13
20/18
20/14
18/8
na
20/11
na
20/12
na
20/2
20/8
20/3
20/1
20/8
20/17
20/6
20/9
20/2
20/1
20/4
20/4
20/4
na
20/5
20/9
20/16
20/1
20/6
20/3
20/22
na
20/5
20/3
20/21
na
20/2
20/2
20/6
20/1
20/4
na
20/3
20/11
20/4
20/3
20/2
223-229
na
267-285
269-273
185-191
167
218
220-264
333-339
na
255-271*
243
258-270
257-261
168-210
154-156
259-305
231-263
283-315
255-315
209-247
205-239
191-209
na
176-214
na
249-275
na
310-312
349-367
114-122
94
171-193
169-221
318-332
314-334
110-112
113
177-183
182-204
240-249
na
170-178
148-172
244-312
210
163-171*
140-144
215-268*
na
184-205
164-176
238-306
na
307-309
302-304
261-271
269
256-266
na
170-174
172-194
243-255
198-202
189-195
13
0.4
0.8
0.0
0.6
0.0
0.0
0.8
0.1
0.8
0.0
0.4
0.4
0.9
0.4
0.9
0.5
0.8
0.8
0.9
0.8
0.8
0.8
0.8
0.1
0.8
0.6
0.0
0.8
0.9
0.6
0.8
0.2
0.0
0.5
0.1
0.5
0.5
0.8
0.9
0.0
0.6
0.3
0.9
0.6
0.2
0.9
0.0
0.1
0.7
0.0
0.6
0.3
0.8
0.5
0.1
0.0
Soc948
Soc949
Soc950
Soc951
Soc952
Soc955
Soc959
Soc960
Soc962
Soc963
Soc964
Soc965
Soc966
Soc967
Soc968
Soc970
Soc971
Soc973
Soc974
Soc975
Soc976
Soc978
Soc979
Soc983
Soc985
Soc987
Soc988
Soc989
Soc991
Soc992
Soc993
Soc994
ATGGACTAAATGGCGAGTGC
GTGTGCGCACGAGTGTTAAG
ATGCAACATCTGTGCAATGG
GACACAGTGGTGGCTGACTG
TGAGGCTGGTTACCCTCAAGG
TCAAACAAGGCTGCTCACTC
GTGCCTGGAGGAAAGACATC
TCAAAGATGACAAATGAATGACC
AGTGTTGATGGACTTGGATGG
GCTATCCTGACATGCCCTTG
ATGCCATTTGTTTCCCTGAGC
TGAGTTGGAGGACCTGTGAG
ACCCTTCTGCTCATCGTCAC
TTGTCCTTCCAACTGGTTCC
GCAATCTTCACATTCACG
AAGAGAAACAAGGACCGAACC
AAACACTCATCCTCTCATCC
GACGACAGGTGTGACAGTGG
CATCTTCCTCAGAGCCAGACC
CCGGATTATTCAACTTCACG
CCCTTGTCCTGCATTTAACC
CCACTTCCCTGCATTTTCC
CTCCTGGAGCTTGTAGTCTGC
CTGCTCTGACTGGGACAAGG
CGACAGTATGTTGACACATCTATGC
GCACCAGCTTGAGTTACAGC
GGCAACATGTTCATCAGTCC
TCGCTGATGTGTCTCTACGG
TCATGACACACTGATTATAGACG
TGCAAAGTAAAGACATAATCCTTCC
CATGGTTGCTCGTAGATTGG
GGGCAGATATTTGGCAAAGG
ATTTGAGGGTGTTTGCATCC
AAATGCAGACAAACACACAAGC
TCAGTGGGGATCAATGAAGG
TCTTCATCCTGCTGTGAGTCC
CAGACAAAACTGGAAAATACAGAGG
TGAGGTGCGTTCACAGTCC
TGACAGGTGGGTTCATCAGG
AAGGACAGAAGGACACACACG
CCACCTCCCTGGAAACTATACC
AACACCAACCGTAGAATGTGC
CTGGTGATTGCCATAAGTGG
ACACCTCGCTCCCTCTTAGC
GGTGGAGCAACACAAACACC
TGCTGACACATCAGTTACACTCC
CATATATCCCTTGGTCTGTGTGC
CCGCTAACAAGCTACAGCAG
GAAGGAACCAAAACCCACAG
CCCCTCCTGTCTATCTTCTCC
CAATCTGCCGTCATTAGAGC
CACCACATTATCGACACAAAGG
TGGGTTCTATGCAAAAGTGC
AGTCACATGAACAGCAAAACG
GACAATGTGCATGAAACAAGC
ACAGCACGAGACACTGAAGC
CTTCACCTGTCGACATCACC
GGGCTTCTATGTACACACTCACG
AATGGTCCTTGGTTGTCAGG
TGGGTTCATTGACAGAGTGTG
TGATGTGGCTGCTTCCTAGA
ATACAAACAGACGGGCTTCG
TCAACCCACGCAGATAATCA
CAAAGATAACCAGACACCAGAGG
GCTTATATCTACGTCTATGCACACG
(TG)7
247
JQ235342
(TG)13
200
JQ235343
(CA)10
161
JQ235344
(GT)12
245
JQ235345
(AC)24
219
JQ235346
(TC)544bp(AC)13
2bp(AC)6
(TG)5
217
JQ235347
220
JQ235348
(GT)13
152
JQ235349
(CTC)6
150
JQ235350
(TG)6
228
JQ235351
(CA)12
181
JQ235352
(TG)76bp(TG)5
249
JQ235353
(GT)42bp(GT)3
159
JQ235354
(TG)514bp(TG)17
150
JQ235355
(CA)13
135
JQ235356
(TG)10
246
JQ235357
(GT)9
190
JQ235358
(CA)13
169
JQ235359
(AAC)5
172
JQ235360
(AC)2035bp(CA)6
201
JQ235361
(AC)9
197
JQ235362
(TG)5
209
JQ235363
(GT)9
292
JQ235364
(AC)31
158
JQ235365
(CTC)8
198
JQ235366
(AC)7
239
JQ235367
(CA)26
235
JQ235368
(AC)15
225
JQ235369
(TC)732bp(CA)12
281
JQ235370
(GT)19
217
JQ235371
(CA)6
192
JQ235372
(TG)11
158
JQ235373
19/5
20/1
20/2
20/7
20/2
20/9
20/2
20/8
20/4
20/16
20/7
20/11
20/4
20/1
20/4
20/7
14/4
20/3
na
20/2
na
20/9
20/1
20/5
20/9
20/1
20/2
20/9
20/4
20/7
20/1
20/5
na
20/6
20/3
20/13
20/19
20/2
20/2
20/8
17/5
20/3
20/11
20/8
20/13
19/3
na
20/21
20/7
20/3
20/5
20/6
na
20/17
20/1
20/9
na
20/7
na
20/12
na
20/3
na
20/10
na
201-209
268
269-273
222-236
211-215
182-230
130-132
259-283
223-233
211-263
199-231
235-277
223-231
243
204-216
168-190
166-182
169-175
na
249-269
na
201-223
182
263-275
267-295
182
185-195
164-194
164-174
145-161
130
262-274
na
204-226
180-202
187-225
168-246
190-193
155-158
204-224
190-210
218-222
206-234
229-243
228-272
313-317
na
146-218
131-161
216-222
212-224
259-275
na
216-264
216
229-265
na
215-231
na
220-270
na
212-216
na
180-218
na
14
0.5
0.0
0.0
0.7
0.1
0.5
0.1
0.6
0.4
0.9
0.8
0.8
0.6
0.0
0.7
0.7
0.5
0.1
0.0
0.4
0.0
0.7
0.7
0.0
0.1
0.8
0.4
0.7
0.0
0.6
0.5
0.1
0.9
0.9
0.5
0.1
0.7
0.7
0.3
0.8
0.7
0.9
0.5
0.9
0.5
0.3
0.6
0.6
0.9
0.0
0.8
0.7
0.8
0.2
0.8
Soc995
Soc998
Soc999
Soc1000
Soc1002
Soc1003
Soc1005
Soc1006
Soc1007
Soc1008
Soc1009
Soc1011
Soc1012
Soc1015
Soc1016
Soc1017
Soc1020
Soc1022
Soc1025
Soc1026
Soc1027
Soc1029
Soc1031
Soc1032
Soc1033
Soc1034
Soc1035
Soc1036
Soc1037
Soc1038
Soc1040
Soc1041
Soc1042
TCTTGATTGAATCCCCTTTTG
CAATTTGCTTGCCATACATATTTT
GGTCAGTGCAGATCAACAGG
TGAAAGACCACCAGAGTAAATCC
GTATGGTGGAGCATGTCTGG
GATGACTCCCAGATGTTTTATGG
GAAGGGTCACAAGCCAAAAG
TGACAGGCTGCTCACAGAAA
TGAAATATCGTGTTGTCACAGATG
TATCGCGACATACATCAACG
GGTGGTAGCGTCGTTGTAGC
ATGATGCGTGGAGAGACAGC
GGAAGTCATGGGGTTAATGC
TGGACAAACACAAATGAATGG
TGTTTGTCCACTGTAATTCAAACC
CAGGGGTGCAGACAAAGC
GGCAGTCCATGATGTCACC
CCGCTCATATTTCACACTCC
TTGATTTACAGACAGATTTATCAGC
GAGTGTGTCAACACATTTATTTCC
CCTGCTCCAGAGCTGCAC
TCAACGATCAAGCATCAAGC
ACAGCACAGCAGAACAGACG
ACTCCATCCAGGTTTGAACG
AGTGTCGGAGCACATCAGC
AGTGTCGCTCCAATCAGAGG
AGAATCCAGGAGTTTGACTCG
CCTTCTGGACTTTGCTCTGG
ACTCCATTCATGCCATCTCC
TGCAGCTGACACTAAACAAGG
GAGATTGACAGGGGACAAGC
TCAGAAATCAAGAGGCTCAGG
TTCTTTGTCAGCCTGGTTCC
CATAGAGGCAGGCAGTGAGG
CTATGGTGTTGGTGCAGACG
ACCTTCTGGGAGCTGATGG
ATGTTTGGAGCAGTGTTTGG
CTACCTCAAGGGGACAGAGG
GAATCGCAAATCACCACTAATAAC
TGTCCATGGGCAAGATACTG
CGACAGCCTTGAAAGTCAGC
CCTGGACTGTCGCTCCAAC
CGTCACACAGCAAACATGC
GGCGATAACACAGGTTCTCC
CAAACCTCGAATCTTCACACC
TCCAGTGGAACCTCAACTCC
GCCCTCATCCATTCATTCC
GGAAGGACCCATTCATGTCC
CTCTTTCTTTCTGCATCATTGG
CCTGGTGTCACCTCTGTGG
TGAGCCAGCATGGTAGAGG
CGAACTAACAGATCATCAAATCG
ATGGCACTGTGTGAAAATGG
ACTCATGAGAGTGCCCTTGG
AGGCTGCACAATCAGAGACC
GGGAAACCACTGTTTAATCACC
AGCCCTCTAACCCTTGTTCC
GTCTTGTTTGGCTTGTCACG
AAAAGTTTGTTGGCAGACTATGG
GCCAGCACTGACAGAAGTAGG
TGCTTTGTCACACATGAACG
CTGCTGGACAGACTCACTGG
TGCCATCTGGAAACTGACC
ATGGAAAGGTCCCATTACCC
GTTCCTGGTGGAACAACTCG
(TG)142bp(TG)13
217
JQ235374
(CA)9
187
JQ235375
(CA)23
184
JQ235376
(TG)6
230
JQ235377
(GT)7
226
JQ235378
(AC)8
195
JQ235379
(TG)5
182
JQ235380
(ACTC)7(CA)8
205
JQ235381
(AC)15
217
JQ235382
(TG)18
217
JQ235383
(CA)1331bp(ATG)5
189
JQ235384
(TG)11
236
JQ235385
(TG)13
234
JQ235386
(AC)14
143
JQ235387
(AC)17
216
JQ235388
(CA)10
191
JQ235389
(CA)24
314
JQ235390
(TC)12
285
JQ235391
(TG)751bp(TG)5
358
JQ235392
(CA)6
299
JQ235393
(GT)15
178
JQ235394
(GT)55
312
JQ235395
(GTAT)4
223
JQ235396
(TG)121bp(GT)20
306
JQ235397
(TG)11
139
JQ235398
(TG)115bp(GT)13
293
JQ235399
(TG)23
138
JQ235400
(TG)6
372
JQ235401
(TG)28
141
JQ235402
(CA)8
313
JQ235403
(CA)6103bp(CA)26
317
JQ235404
(AC)87bp(CA)5
326
JQ235405
(AC)13
321
JQ235406
20/16
20/3
20/3
na
20/12
20/19
20/2
20/1
20/4
20/6
20/5
18/2
20/1
20/2
20/4
na
20/17
20/1
20/10
20/1
20/6
20/1
20/9
20/11
20/13
20/11
20/12
na
20/13
20/3
20/4
20/1
20/16
na
20/6
20/3
20/3
20/1
20/6
na
20/14
20/6
19/15
na
20/3
20/5
20/18
20/1
20/6
20/1
20/17
na
20/9
20/1
20/1
20/3
20/11
20/2
20/2
na
20/10
19/11
20/6
na
20/5
210-250
210-216
207-211
na
196-252
184-254
252-260
240
249-255
256-272
212-222
168-172
205
205-207
220-228
na
237-287
227
234-254
231
196-214
192
256-282
255-275
256-284
224-258
142-178
na
245-277
235-239
213-219
208
324-362
na
296-312
286-292
380-384
372
319-339
na
188-222
196-208
238-326
na
242-244*
240-249*
286-340
274
153-165
144
288-326
na
155-171
129
397
370-388
133-167
125-127
310-314
na
308-350
298-332
348-360
na
324-348
15
0.9
0.0
0.3
0.9
0.9
0.1
0.0
0.5
0.6
0.7
0.2
0.0
0.3
0.6
0.9
0.0
0.8
0.0
0.6
0.0
0.8
0.8
0.9
0.8
0.8
0.8
0.2
0.2
0.0
0.8
0.7
0.5
0.4
0.0
0.4
0.9
0.6
0.8
0.5
0.7
0.9
0.0
0.7
0.0
0.9
0.8
0.0
0.0
0.4
0.8
0.4
0.0
0.8
0.8
0.7
0.6
Soc1043
Soc1044
Soc1046
Soc1047
Soc1048
Soc1050
Soc1051
Soc1052
Soc1054
Soc1056
Soc1057
Soc1058
Soc1059
Soc1060
Soc1061
Soc1062
Soc1063
Soc1064
Soc1065
Soc1066
Soc1067
Soc1068
Soc1069
Soc1070
Soc1071
Soc1072
Soc1073
Soc1075
Soc1076
Soc1078
Soc1079
Soc1080
TGACCTCTGTGACCTGAGTCC
CTTTAGGAAAGGGTGCTTGG
ATGTGTGTTTCCTGCTGAGG
AGAGTTGGATGCTGACACTGG
GTGTTGCTCCCTTTCTCTCG
AGTTTGAGGCAATATCAAAAAGC
TTCAGTGATTGCCAGTGTGG
GCACCAGCTGACGAATGG
GTGCCTGCTATGGAAAGTGG
TTTTCCCTTACGCACAAGC
GAGCAGCTCACAGTTGAAGG
TTTTATTCCATTCTTTTTACATCACG
GGATTCAGACCTGGCTGACC
CACACAGCTGAGGTTTCTGG
TTGCTGTGAACAACCTGACC
CAACCTCGTCAACCAGAAGG
TGTTTGCTACCTGTGTCATGG
TGAACGTAGCGTGGACAGG
AACTATGTGCCAGGGTGAGC
AGTGACACCTGCAAAGTGACC
ATGTAGCGCGCTCAAGTCC
CTCTGAAGAAGACGCAGAGC
CAAATGACGAGTGACACAAGC
TGGATCTGGTCTGGATCACC
AACCGTGGATGATTGACAGC
TGGTGTCCTCACTCATCTGC
CTAATTGTGCCTTTTCCATGC
CGTCCCTTCATGAACATCTG
ACACCACCTTGTCCGAAAAC
ATTCAAACTGTGGGCAGAGC
TCCGTCCGTGTCTCTTTAGC
TTGATTTTGAGCGACTGAGC
TCAGCTACATCAAAGCAACG
GGCTGTCCCCATTACTGC
TCAGCACCAGTAAACCTTGG
AAAAAGCAAATGCAATAACTACAGG
AGGAGACAGGGATGAATAGGG
AGATGAGGCGAGGGAAGG
TCTCCAATCTGTCGTGATGC
CCACCATTGTTCCCTTTCC
TTCCTTGAAGAGGCAAGAGC
GGGCCCTGTTTCTGTTACG
TTTCATCCCTCGTCGTATGG
ATCCAACCAAAGACAAACACC
TGACTTGAGGAACTCCAGAGC
CAGAGAGCAGCTACCACACG
CGGGAACAGATGGAGTAACG
ATGGCTGCAGACTCTTCTCC
GCAGCAGTTACTGTTCGCTTC
GTATGTGCGGCCTGTATGC
TCAAAGTCAAAACAGAGGAGAGG
AATGTTTGTGCAAGGCTTCAG
CCGACACTTTTGTTGTCGTG
GATTCCCTCATCCTGTTTCG
TAAATCCCCCATGACTGAGC
AACCTGCATGTGAGTGAACG
ACTTTGCAGTGTGCACTTGG
TGATTAATGCTCTTAATTCCTCTCC
CGAAGGCTTTTAGTGTCTAGTGG
ATGGCGAATGTCATCAACC
AGAGCAGGTTTTGCTTCTGG
AATTTACCACCTGGCAGAGC
TCATTGTCCTTTCCCTTTGG
AAATGTTATGAGGGCGATCC
GAACAGGTTTGGGAAATTGG
(AGAA)6
320
JQ235407
(TG)133bp(GT)9
58bp(TG)5
(TG)9
261
JQ235408
212
JQ235409
(CA)8
145
JQ235410
(CA)64bp(CA)9
217
JQ235411
(GT)17
253
JQ235412
(CA)321bp(AC)5
282
JQ235413
(CA)29
293
JQ235414
(TC)9
310
JQ235415
(CA)523bp(TG)9
61bp(GT)9
(AC)44
214
JQ235416
269
JQ235417
(AC)61bp(CA)12
167bp(CA)19
(CA)6
347
JQ235418
313
JQ235419
(GT)26
218
JQ235420
(GT)13
334
JQ235421
(GT)7
151
JQ235422
(AC)12
166
JQ235423
(AC)23
374
JQ235424
(GT)1143bp(TG)592bp
(GAT)6(GGT)7(TG)15
(CA)67bp(AC)6
357
JQ235425
163
JQ235426
(GT)66bp(GA)5
108
JQ235427
(TG)63bp(GA)10
140
JQ235428
(CA)15
207
JQ235429
(GT)13
167
JQ235430
(TG)23
95
JQ235431
(TG)10(GT)8
332
JQ235432
(TG)5
269
JQ235433
(AC)15
112
JQ235434
(AC)134bp(AC)27
182
JQ235435
(TG)5
193
JQ235436
(TG)12
239
JQ235437
(TG)9
307
JQ235438
na
20/5
20/3
20/9
20/9
20/5
20/7
20/3
20/10
20/6
20/9
20/22
na
20/23
na
20/24
na
20/3
20/1
20/9
20/6
20/24
na
19/18
19/14
17/5
20/1
20/21
20/1
20/8
20/7
20/4
20/15
20/7
20/1
20/17
na
20/16
20/16
20/2
20/2
20/2
20/12
20/6
na
20/19
na
20/25
na
20/23
20/13
20/13
20/14
20/2
20/1
20/11
20/6
20/19
20/16
20/3
20/1
20/5
20/2
20/4
19/4
na
290-342*
235-241*
283-301
336-360
235-243
229-243
164-172
163-181
200-224
200-226
279-369
na
269-326*
na
275-355
na
327-335
327
230-248
220-232
234-320
na
340-398
322-350
332-342
348
231-281
203
345-363
345-361
175-183
152-202
171-205
166
379-411
na
360-402
370-416
183-185
168-176
127-129
127-153
159-169
na
220-266
na
163-243*
na
103-183
93-129
350-377*
334-359*
283-291
294
122-154
127-141
162-228*
141-191
208-216
216
234-264
208-222
326-334
289-309
16
0.6
0.4
0.7
0.7
0.4
0.5
0.3
0.8
0.7
0.6
0.9
0.9
0.9
0.5
0.0
0.8
0.7
0.9
0.9
0.9
0.7
0.0
0.9
0.0
0.8
0.7
0.4
0.9
0.3
0.0
0.9
0.9
0.9
0.2
0.0
0.2
0.8
0.6
0.9
0.9
0.9
0.8
0.8
0.9
0.0
0.0
0.8
0.7
0.9
0.9
0.2
0.0
0.6
0.4
0.3
0.2
Soc1081
Soc1082
Soc1083
Soc1084
Soc1085
Soc1087
Soc1088
Soc1089
Soc1090
Soc1091
Soc1092
Soc1093
Soc1094
Soc1095
Soc1096
Soc1098
Soc1099
Soc1100
Soc1101
Soc1102
Soc1103
Soc1104
Soc1105
Soc1106
Soc1107
Soc1108
Soc1109
Soc1111
Soc1114
Soc1115
Soc1117
Soc1118
Soc1120
ATCCTCACACTCCCTCATGG
CTGGCAACCCAGTGATAAGG
AGGAGCCTCTCACTACCAACC
GCAGTGATACTATTTCATTGTCTTAGC
CGAACACTGCGCATTATAGC
AGCTGTGAAAATGAACCAAGC
CACACAAGCACCATCTCTGG
GAAGTGGAGAGCCTCACTGG
GCAGGTGGAGAAAGAGACTTACC
TCAGTCTGCAGCTATGTGTGG
TTCCAGGGGCGTATTTAGC
AACCTCCTCCTGAAACATGG
ATGTGAATGCATCCGAGTCC
GATGATCCAAACAGGAAATTGG
TGTTACCACGGCAACAAGC
TTCTGTGGTGGTCGGTAAGG
ACTCACATCTCGACAAACATAGG
TGATACCTCAGCAGTCTCTTGG
ACTCGGAGGCTTTTCACTCC
CCATATGGGAGGGCTACTGC
TTTCCCTCCATTCAGTTTGC
CCCTGTCATGCATCATTCC
CACGTAACAAAGCCGTCTCC
TTTTGAAGGAAGCCACATGC
TGTAACCCCACAAACAGAAGC
AGCAAACCTCACCTCTGACG
GAGTCAATTCCTGCACAGAGG
CACCAGGACGAACAATAAAGC
GGACAATCGCTACACCTTCC
GTGAAATATGCACCCAAAAGC
ACCAAAGATTGCCTGAATGG
TCCTTCCGTGTCACATTGG
AATGTGACACGGAAGGACAG
GGACCGTCAGATCCTACAAAC
AATGCATTCCCTGTCCTACG
CTGGTGTGGACCCATAAAGG
CTGAGAATGATGAAAGCTGAAAG
GCGCTAGCGTAGCACAGTC
TGTTTCCATGGTGAATCTGC
CCTTGAATCGGTCCAACTAGG
GGGGAAAAACAGGTCACTGC
CTGCGCTCCTATTCACACG
CCCCACTCCTAGACTGATGC
ACTGAGAACACTGCCACTGC
CAAACCTCCTCCAGCTTCC
GTTCACACCCAAACACATGC
CCTGAGAAGTAGGCAGACTCG
TGCTGTAACTGGAATCAACTGC
GGTGGTCGACCTCTGTGC
AGCAGAGTCAACAGAGAGTCTGG
GTGTGAATGTTGCCATCTGC
CATCAGTGTATTCACAGTAAATGAAGC
TTTCTCTTCAAAGGATCACACG
CACACTTGAATCCCCTTGC
TGGCAAAACACCAAACACC
AAGAAATCGAGACGCAGACG
GCCTTTTGTTGAGTGGAAGG
GGCACTGGTCACAGAAAGG
CAATAGATTGGGGATGAGTGC
GCTTTCTCAAGCTTCTCTGAGC
GCAGTTGCACAGGAAAAGC
GATGAGTCACTGCGTCAAGG
ATGTGAGGAGTGGGTGATGG
ACAGCGTCCTGGTTAGATGC
AACCCAAGACCACAACATCC
(GT)21
299
JQ235439
(GT)17
139
JQ235440
(AC)18
279
JQ235441
(CA)8
252
JQ235442
(AC)1016bp(AC)6
142
JQ235443
(AC)27
287
JQ235444
(CTG)538bp(GA)11
366bp(GT)32
(CA)14
306
JQ235445
194
JQ235446
(GT)239bp(TG)52bp
(TG)7
(GT)16
218
JQ235447
120
JQ235448
(AC)9
338
JQ235449
(TG)12
344
JQ235450
(CA)156bp(CA)11
10bp(CA)13
(TG)8
279
JQ235451
234
JQ235452
(GT)71bp(TG)6
288
JQ235453
(GT)11
285
JQ235454
(GT)13
204
JQ235455
(AC)6
287
JQ235456
(GT)14
350
JQ235457
(GT)69bp(TG)9
124bp(TG)8
(TG)5(AG)9
294
JQ235458
129
JQ235459
(GT)17
119
JQ235460
(AC)52bp(AC)11
373
JQ235461
(AC)13
291
JQ235462
(AC)15
110
JQ235463
(AC)19
242
JQ235464
(TG)1189bp(AC)9
227
JQ235465
(AC)22
180
JQ235466
(GT)5
113
JQ235467
(CA)7
294
JQ235468
(CA)10
276
JQ235469
(TG)5
145
JQ235470
(AC)6
173
JQ235471
20/20
20/8
20/12
na
20/8
20/2
20/3
20/1
20/4
20/3
20/19
na
20/26
20/11
20/7
na
20/13
na
20/10
20/13
20/5
20/4
20/20
na
20/8
20/1
20/3
20/1
20/2
20/8
20/7
20/1
20/14
na
20/2
20/2
12/8
na
20/4
20/5
20/5
20/1
20/5
20/8
20/11
20/6
20/3
na
20/3
20/4
20/9
20/1
20/7
20/1
20/19
na
20/4
20/1
20/5
20/1
20/2
20/3
20/1
20/3
20/2
338-388
262-282
146-172
na
294-318
265-275
272-276
271
154-164
153-157
208-344
na
307-376
283-309
204-216
na
202-248
na
135-157
123-165
350-364
354-364
361-395*
na
294-344
244
256-260
252
310-312
316-336
292-306
316
213-239*
na
290-308
299-309
365-381
na
319-325
321-329
145-153
132
135-149
113-127
369-399
355-371
303-313
na
130-134
107-115
253-273
192
246-258
223
176-242
na
133-139
121
313-325
293
299-301
267-271
166
163-169
193-195
17
0.9
0.7
0.8
0.8
0.0
0.2
0.0
0.5
0.3
0.9
0.9
0.7
0.7
0.8
0.8
0.8
0.4
0.2
0.9
0.8
0.0
0.2
0.0
0.0
0.8
0.7
0.0
0.8
0.0
0.0
0.8
0.6
0.7
0.6
0.0
0.7
0.8
0.8
0.7
0.2
0.5
0.5
0.8
0.0
0.8
0.0
0.9
0.4
0.0
0.6
0.0
0.0
0.4
0.0
0.5
0.0
Soc1121
Soc1122
Soc1123
Soc1124
Soc1125
Soc1126
Soc1127
Soc1128
Soc1129
Soc1130
Soc1133
Soc1134
Soc1136
Soc1137
Soc1138
Soc1139
Soc1140
Soc1141
Soc1142
Soc1143
Soc1144
Soc1145
Soc1146
Soc1147
Soc1148
Soc1151
Soc1152
Soc1153
Soc1154
Soc1155
Soc1156
Soc1157
AAACTGCAAATCGTTCACTCG
CATGCCAACACAAACACTGC
AGAGGGTCTGGATGCTTGG
GTTGGAGGCTCTGGACTACG
TCACGTCAGTTTCCTGTTTCC
ACCAGCGAGACGATAACACC
AAGAGATCAGCGGCTAGTGG
TGGCAGCAACTCTACTGTGG
TGCAGACAGGGAAACTTGC
CCAGATGGAGGTTGTTAGAGG
CGTGCCAATCTGTAAAATGC
GGGTTTGGTCACGTAGACTAGC
GATTACAGGTGTGGGTGATGC
CAGGGAGGGATGATTGAGC
GTGTCAGCATGGAGTCATGG
AATCTGCCGTTCAGATCC
ATGTGAGCTTGTTCTCATGC
TTTTCCCCATCAAATGTATGC
GTGTGACCACCTGCTGAGG
CACAAACTGGTGCTCAGTATCC
TCAAACAGTCACCGTCATCC
AATCAGGCACTTCCTTTTCC
CATGTCTGAAGACTGACAGTTAAGC
ATGTGCGTGTTAAGCTGCTG
CTCATTCATGCATAGAAAATGTTTG
GACCTGAACCTCAATGAAACC
AGCCCGGTCTGATAAAACG
CACTTCGCTCACAAACTTGC
CTCAGAACCCAGGGAAAGG
CCCGGTGTATTGTTCTGACC
GCCAGAGTGTGTTTAAAAATTGG
CACGAGTCTGGCTTTGTGG
GTCTCACCGACACCTTTTCC
AATTACACAGCTCTGGACTACGG
GGGTGTGATGGAACAGAAGG
TTTGTTGTTGACCGTGATGC
GGGCAGCTAACATGAACAGC
AGTGTGTGTTTGCACTCTGC
GTTTGACTGAAGTCAGAAACG
GTGGAATCTGGCACAGTGG
TTTGACGCAACAGCATTACC
CAGTGATCCATGAACCTTGG
AAGGCCTAGACCCTTATCTTCC
TTCAGCTTCACTTCTGTGTCG
AACCAGTGATGTGAATGAAAGG
CATCACTGGTTTGGATTTGC
GGTGAGACATGTGGATGTGG
AGTCATAGCCGAGGATCTGC
ACAGCACAGCGCTTAGACG
TCGTGAATGCTTCAAACAGG
CGTTAGAGTTGCCCACAAGC
TTCACCCCACCATCACTACC
AAAATGCCCACATTAATTCTCC
CCCTCCCTACTGTCCTGTCC
TCAGCACAGCAGTCAGATCC
GGCTGAATTACTGGAACATGC
TTGAGCACTACCCAACATGC
CCTCCTTTCACCTCCAAACC
AACAGCGTGCAACAAGTCG
TGCAGAGGGATAGACATGACC
TGGCTAAGTGGAGCTGAAGG
TTAGGGACACACCACTGTGC
GCTTTCACACCCACTTTTCC
CATATCCATGGCAACAGAAGG
AGGTAAAGAGTGGGCTCATCC
(AC)8
260
JQ235472
(TG)7
124
JQ235473
(AC)5
267
JQ235474
(TG)13
316
JQ235475
(TG)25
256
JQ235476
(TG)19
181
JQ235477
(TG)22(CTG)11
218
JQ235478
(CA)24
99
JQ235479
(CA)82bp(CA)5
167
JQ235480
(GT)6
225
JQ235481
(TG)13109bp(GT)9
247
JQ235482
(TG)131bp(GT)5
189
JQ235483
(TG)11
331
JQ235484
(CA)8
130
JQ235485
(AC)9
181
JQ235486
(GT)7(GTGC)7
224
JQ235487
(TG)8
94
JQ235488
(GT)8
300
JQ235489
(TG)51bp(GT)18
281
JQ235490
(AC)151bp(CA)5
277
JQ235491
(TG)7
349
JQ235492
(CA)7
140
JQ235493
(GT)8
291
JQ235494
(GT)6
84
JQ235495
(TG)24
234
JQ235496
(AC)73bp(CA)6
106
JQ235497
(TG)13
129
JQ235498
(TG)9120bp(GT)5
286
JQ235499
(CA)18
296
JQ235500
(GT)62bp(GT)10
263
JQ235501
(GT)52bp(GT)6
151bp(GT)15
(TG)51bp(GT)17
(GA)6
341
JQ235502
290
JQ235503
18/6
20/3
20/2
20/2
na
20/2
20/1
20/6
18/1
20/13
20/4
20/8
20/1
20/14
20/13
20/16
20/1
20/6
20/2
20/1
20/2
20/10
20/12
20/12
20/6
20/11
na
20/8
20/12
20/2
20/5
20/11
20/14
20/4
20/4
20/2
na
20/9
na
20/9
na
20/4
14/2
20/4
na
20/2
20/4
20/2
20/2
20/14
20/1
20/5
na
20/11
20/1
20/3
19/2
20/17
20/3
20/6
20/8
20/10
na
20/11
na
193-213
261-283
260-266
145-147
na
285-289
296
336-346
323
263-293
246-254
213-237
253
238-290
191-231
102-148
102
179-193
178-180
246
213-219
269-305
353-383
194-224
183-207
346-378
na
145-169
134-168
200-202
211-225
247-281
232-270
117-129
122-134
324-326
na
286-308
na
293-312
na
361-373
379-381
155-173
na
311-313
313-319
103-105
102-104
230-262
193
117-135
na
141-179
120
302-310
269-275
301-343
315-319
279-291
280-298
343-371
na
291-321
na
18
0.6
0.1
0.0
0.0
0.0
0.0
0.7
0.0
0.9
0.3
0.8
0.0
0.8
0.9
0.8
0.0
0.6
0.1
0.0
0.0
0.8
0.8
0.8
0.5
0.8
0.5
0.8
0.0
0.7
0.8
0.8
0.4
0.6
0.3
0.8
0.8
0.2
0.0
0.1
0.4
0.5
0.4
0.4
0.8
0.0
0.6
0.7
0.0
0.4
0.1
0.9
0.3
0.7
0.7
0.8
0.8
Scoc02
CAGGTTCCAGCACATTTTAGC
AAAGATGCGATTGTTGATTCG
(CA)11109bp(CA)9
223
EU726996
20/5
20/5
230-246
234-244
173
174
Msat is the designation of the microsatellite marker.
175
Primers are the forward (top) and reverse (bottom) primer sequences.
176
Motif indicates the repeat motif.
177
Clone Size is the size (in base pairs) of the allele in the sequenced clone.
178
GenBank is the GenBank Accession number for the clone sequence.
179
N is the number of individuals assayed, and NA is the number of alleles detected.
180
181
Size Range refers to the alleles thus far uncovered (includes the 21bp 5’-tail-sequence); * indicates that
alleles are not spaced as anticipated, i.e. 1 bp steps between allele sizes in a dinucleotide marker.
182
HE and HO are expected and observed heterozygosity, respectively.
183
184
PHW represents the probability of deviation from Hardy-Weinberg expectations; significant deviations,
following Bonferroni correction (Rice, 1989), are underlined.
185
186
187
MICRO-CHECKER refers to possible issues with loci as indicated by MICRO-CHECKER (Van Oosterhout et al.,
2004): N represents evidence for null alleles, S represents evidence for scoring errors due to stuttering,
and – represents no evident issues.
188
na indicates that the marker failed to amplify consistently.
189
19
0.6
0.4