Pertanika Vol. 8 No. 3 December 1985 PERT-0314-1985 Chemical and Mineralogical Characteristics of an Organic Soil (Troposaprist) from Sg. Burong, Tg. Karang, Selangor J. Shamshuddin, Nik Mokhtar and A.J. Kamal Keywords Organic soils; minerals; charges; soil buffering Abstract At bulk density of 0.97 g/cm 3 and pH (top soil) of around 5 reflects that the soil has improved after undergoing an ameliorating programme and more than a decade of cultivation. But at depth, pH is still low where AI is present in large quantities. A high water table leads to the accumulation of bases, especially Ca and Na in the lower horizons. Low pH and high CEC is the reflection of high amounts of organic matter and mica-vemiculite-smectite mineralogy. PERT-0315-1985 The Lead Tetra-acetate Oxidations of Some N-Arylaminofumarates in the Presence of Trifluoroacetic Acid Mohd Aspollah Sukari Keywords Lead tetra-acetate; oxidative cleavage Abstract p-Chloroanilino-, p-acetylanilino-and p-toluidino-fumarates are oxidized by lead tetraacetate in low yields to give oxanilate. Similar oxidation of p-acetylanilinofumarate in the presence of trifluoroacetic acid affords only oxanilate. p-Toluidino and p-chloroanilino-fumarates with lead tetra acetate/ trifluoroacetic acid give mixtures of oxazin, azobenzene and anilide. PERT-0316-1985 Tolerance of Soybean Rhizobia to Soil Acidity R.A.Jones and J.E. Giddens Keywords Nitrogenfixation; Rhizobium japonicum; legume inoculation Abstract Some nodule bacteria do not tolerate acid soils. A study was initiated to determine if Rhizobium japonicum could adapt to soil acidity and therefore become more effective N -fixers in association with soybeans (Glycine max L. Merr.) grown in acidic soils. Nodules were obtained from soybeans grown in fields where the crop had been planted for at least 5 years in soil with pH near 5, 6 or 7. The nodular material was used for inoculation of soybeans in a factorial experiment grown in methyl bromide fumigated soil obtained from the same fields as the inoculum. Nitrogenase activity (C2H2 — C2H4 reduction) and plant weight were used for evaluating the treatment effects. The results seem to indicate that the nodular bacteria did not adapt to a given soil pH. Soybeans grown in soil at pH 6 grew best regardless of whether inoculum was from plants grown at pH 5, 6 or 7. In acid soil, inoculum from soils at pH 5 was no better than those from pH 6 or 7. PERT-0317-1985 Accumulation and Migration of Phosphate Applied as Rock Phosphate in an Oil Palm Plantation A.R. Zaharah, J. Hawa and H A H. Sharifuddin Keywords Total P; P fractionation; extractable P Abstract An experiment was conducted to study the accumulation and migration of P applied as Christmas Island Rock Phosphate (15% P) to an oil palm crop grown on Kuala Brang soil series (Typic Paleudult). These oil palm trees were fertilized for 17 consecutive years at the rate of 0, 44 and 88 kg P/ha/year. Most of the P was found to accumulate mainly in the top 10 cm of the soil and were present in the order of Ca-P > Fe-P > Al-P. Olsen extractable P, total P, inorganic P fractions, soil CEC and pH were found to increase with increasing rates of P applied. PERT-0318-1985 Characterisation of Bidor Kaolinite and Illite K.Y. Liew, L.E. Khoo and K. T. Bong Keywords Kaolinite; Illite; Characterisation Abstract Two types of commercial clay minerals from the Bidor region of Perak were studied. From the Xray diffraction study, these clays were identified as disordered kaolinite and 2 M polymorph of illite. The morphologies, the surface properties, the pore structures and the infrared absorption properties were examined. The basis for the identification of these clays is discussed. PERT-0319-1985 Soil Fusaria from Oil Palm Habitats in Malaysia Y.W. Ho and G. Varghese Keywords Fusarium; soil; oil palm Abstract Fusarium species were isolated from 22 sampling sites in 4 oil palm plantations located in different regions of Peninsular Malaysia using Peptone-PCNB medium. Eight species and two varieties of Fusarium viz., F. solani, F. oxysporum, F. oxysporum var. redolens, F. semitectum, F. moniliforme, F. moniliforme var. subglutinans, F. equiseti, F. longipes, F. lateritium and F. heterosporum were isolated. Among these, F. solani and F. oxysporum were the most prevalent species followed by F. semitectum and F. moniliforme. The other 5 species and 2 varieties of Fusarium were of sporadic occurrence. Generally, soils from areas with young palms had larger number and greater variety of Fusarium species when compared to soils from mature and old palm areas. The rhizosphere soil contained a greater variety of Fusarium species than soils in the avenue of palms (non-rhizosphere soil). PERT-0320-1985 The Effects of Sorbed Alkali Metal Ions on the Conductance of Activated Carbon M. Badri, Karen Anne Crouse and Harun Awang Keywords Conductance; activated carbon; pores; sorption; viscosity; mobility of alkali metal ions Abstract The rate of increase of the conductance of activated carbon immersed in NaCl solutions was found to be lower when the concentration of the salt was increased. This was probably due to the increase in the viscosity of the solutions which prevented the filling of the pores to the same extent as in the case of pure water. In addition, the final conductance of the sample was also lowered. The opposite effect was however observed in HCl solution. Measurements in the presence of Li + and K + suggested that the mobility of the ions determined the final value. PERT-0321-1985 The Effect of Temperature on the Destruction of Salmonellas in Activated Sludge M. I. Yaziz Keywords Activated sludge; Salmonella; temperature effects of wastewater treatment Abstract The effect of temperature on the survival of salmonellas in activated sludge was examined. The results showed that the Salmonella destruction rates increased in line with increasing temperature of the mixed liquor from 5° to 25 °C, whereas at 30 °C the rate declined markedly. This is most probably due to inactivation of ciliate protozoa at the higher temperature resulting in increased survival of the pathogens. The slow reduction in the number of salmonellas at 30°C may be the result of nutrient starvation, in addition to other possible mechanisms such as the lytic action of bacterial viruses. PERT-0322-1985 A Contribution to the Phytochemical Survey of Peninsular Malaysia Mawardi Bin Rahmani, Ruth Kiew, Nordin Hj. Lajis, Rahim Othman and Robert F. Toia Keywords Phytochemical screening; alkaloid, triterpene/steroids, saponin survey of Malaysian plants Abstract Specimens of 216 plants representing 150 genera and 50 families were collected from four areas in the western and central parts of Peninsular Malaysia. The leaves of each species were screened for alkaloids, steroids and triterpenes, and for saponins. 28 species (13%) gave a positive test for alkaloids, 86 (40%) for saponins and 55 (25%) for triterpenes/steroids. PERT-0323-1985 Prey Selection by Larval Micromus tasmaniae Walker (Neuroptera: Hemerobiidae) M Y. Hussein Keywords Micromus tasmaniae; hemerobiidae; predation Abstract The brown lacewing, Micromus tasmaniae, is the most common predator of potato aphids in South Australia. The probability of capture and prey preference of larval M. tasmaniae were measured for each in star of Myzus persicae Sulzer (the green peach aphid) at constant 15°C, 20° and 25°C. The probability of capture of M. persicae by larval M. tasmaniae increased after each moult and declined just before moulting, especially at 15°C. Except for very small and young larvae, the predator larvae were able to capture efficiently both small and large prey. Young larval predators showed some preference for smaller prey, whereas older larvae exhibited little or no preference. The effectiveness of M. tasmaniae larvae may vary considerably between seasons and climates. PERT-0324-1985 A Study of the Bacterial Population Fluctuations During Poultry Processing M. I. Yaziz and R. Awang Keywords Salmonella; poultry processing; bacterial contamination of poultry carcasses Abstract The microbial assessment of skin-washed water samples taken from 39 poultry carcasses and the wash water from a poultry processing unit revealed cumulative increases in the number of micro-flora at each stage of processing. A comparison of the bacterial population densities on the skin at the start and at the end of processing showed that the levels of E. coli and coliforms increased by 231.1% and 263.1% respectively whereas the Salmonella concentrations decreased by about 9%. A positive correlation was obtained between the occurrence of bacteria and parameters such as wash water pH and temperature, and suspended solids concentration. An examination of the storage tank effluent which was used to store the poultry carcasses prior to packaging showed that E. coli and Salmonella were able to thrive in the liquid. During the 4 h incubation period, the levels of both organisms declined rapidly during the initial half hour, but quickly became adapted and multiplied in the subsequent hours. The implications of both, the field and laboratory studies are discussed with a view of reducing the level of contamination during processing. PERT-0325-1985 A Comparative Study of Leaf Litter Decomposition Rates in a Hill Forest and a Forest Plantation in Peninsular Malaysia Lee Su See and Yong Teng Koon Keywords Leaf litter decomposition; hill forest; forest plantation; litter bag studies Abstract A comparison of seraya (Shorea curtisii Dyer ex. King) and pine (Pinus caribaea var. Hondurensis) leaf litter was made over a period of 16 weeks in a Hill Dipterocarp Forest (HDF) and in a pine plantation (PP). At both sites, seraya leaves decomposed at a faster rate than pine needles. Weight losses after 16 weeks from seraya leaves varied from 19.5% (PP) to 39.0% (HDF) while pine needles showed weight losses varying from 10.3% (PP) to 13.6% (HDF). Soil microarthopods were suspected to play a more important role in seraya leaf litter decomposition in the HDF than in the PP. The significance of these findings on forest management is discussed. PERT-0326-1985 The Effects of Temperature on the Development of Hemispherical Scale, Saissetia Coffeae (Walker) Abdul Ghani Ibrahim Keywords Saissetia coffeae; temperature; growth rate, regression Abstract The rate of growth and size of Saissetia coffeae (Walker) (Homoptera: Coccidae) were studied under seven different constant temperature: 18°C, 20°C, 22°C, 24°C, 26°C, 28°C and 30°C. Results showed that an increase in temperature within limits would accelerate the rate of development for all the stages of S. coffeae. Thus, the development from egg to adult at 18°C and 28°C were 95.2 and 51.2 days respectively. Continuous rearing of nymphal scales at 30°C failed to produce adults indicating lethal effects at this temperature. There was a higher correlation between temperature and growth rate for all the stages of scale using polynomial regression. PERT-0327-1985 Effects of Packing Densities in Plastic Bags on Survival of Larvae and Fry of Helostoma temmincki (C&V) S.S. Siraj, S.H. Cheah and Z.A. Aizam Keywords Packing density; larvae; fry; water quality parameters; Helostoma temmincki Abstract Helostoma temmincki (C&V) larvae measuring 4.6 ± 0.6 mm total length were packed in plastic bags with oxygen at stocking densities of 0, 250, 500, 1000 and 1500/litre. Fry measuring 2.0 cm ± 0.2 were similarly packed at densities of 0, 125, 250, 500 and 750/litre. The fish were observed for mortality 10 minutes, 24 hours and 48 hours after packing. The water quality parameters — temperature, dissolved oxygen, ammonia-N, dissolved carbon dioxide, pH and alkalinity — were determined at 0 hours for control bags only and for all bags at 48 hours after packing. There was no mortality in all bags 10 minutes after packing. After 24 hours, mortality of larvae packed at 500, 1000 and 1500/litre was less than 2% whereas no mortality was observed in the bags with fry. At 48 hours, mortalities of larvae were similar (P > 0.05) in all bags and were less than 2%. Mortalities of fry packed at 125, 250 and 500/litre were similar (P > 0.05) and less than 2%, whereas mortality of fry packed at 750/litre was significantly different (P < 0.05). PERT-0328-1985 Keperluan Latihan Pegawai-Pegawai Barisan Hadapan RISDA Maimunah Ismail dan Asma Ahmad Keywords Keperluan latihan; pegawai-pegawai barisan hadapan; RISDA Abstract Satu kajian telah dijalankan di kalangan 194 orang pegawai-pegawai barisan hadapan RISDA dari seluruh Semenanjung Malaysia. Tujuan kajian ialah untuk mengenalpasti keperluan latihan pegawai-pegawai pengembangan ini dalam bidang teknik dan bukan teknik. Penemuan kajian adalah penting untuk merangkakan kurikulum latihan dalam perkhidmatan bagi kategori pegawai RISDA tersebut. Kajian telah mendapati pegawai-pegawai barisan hadapan RISDA memerlukan latihan dalam perkhidmatan dalam bidang-bidang yang berkaitan dengan program modenisasi untuk memperbagaikan punca pendapatan pekebun kecil iaitu ternakan kambing, lembu dan kerbau (76 peratus), ternakan ikan air tawar (73.65 peratus), ekoi*omipertanian (73.4 peratus) dan ternakan ayam (73.28 peratus). Pegawai-pegawai tersebut mempunyai kecekapan yang rendah dalam bidang-bidang tersebut kerana ia merupakan program baru yang diberi tumpuan oleh RISDA di samping program-program pengeluaran getah. PERT-0329-1985 Differential Pulse Polarography and Voltammetry with an Automated Microprocessor-based Polarograph and a Static Mercury Drop Electrode W. T. Tan and G.S. Tan Keywords Analytical evaluation; pulse; stripping; precision Abstract Differential pulse polarography using an automated, microprocessor-based polarographic analyzer equipped with a static mercury drop electrode has been evaluated with respect to pulse amplitude, scan rate, drop time (t), and drop area. Variation of peak current, ip with drop area and t1/2, with some pulse amplitudes agree reasonably well with theory. However, dependence of ip and E on scan rate and on pulse amplitude at very large and very small amplitudes was found to deviate from theory. Similar trends were also found for anodic stripping voltammetry when differential pulse stripping was used. The precision attainable by both techniques was good. Copper peaks demonstrated good precision only when copper was plated out individually during anodic stripping voltammetry. PERT-0330-1985 Chemical Forms of Cu, Zn, Ni and Co in Soil and Sediment of Ranau, Sabah Marcus Jopony Keywords Chemical forms; sequential extraction; soil; sediment available Abstract This study was conducted to determine the distribution of the chemical forms of Cu, Zn, Ni and Co in the soil and sediment samples obtained from Lohan area in Ranau, Sabah. The samples were sequentially extracted with 0.5 M KNO3 to remove soluble plus exchangeable metals, with 0.5 M NaOH to remove organically bound metals, 0.05 M Na2 EDTA to dissolve metals in oxide or carbonate form, and with 4 M HNO3 at 80°C to dissolve metals in sulphide minerals. Most of the metals analysed were present as sulphide minerals with the exchangeable plus soluble fractions being very low. PERT-0331-1985 Histological Changes in the Pregnant, Lactating and Involuting Mammary Gland of the Guinea Pig W. Nordin and C.S. Lee Keywords Histology; mammary gland; leucocytes; guinea pigs Abstract The histological changes of mammary glands of guinea pigs during mid and late pregnancy, 1 day after parturition, peak lactation and 2, 3, 4, 8 and 12 days after weaning were studied. Rapid growth of the glandular tissue occurred towards late pregnancy and was completed by peak lactation. Plasma cells occurred in large numbers in the interalveolar connective tissue during the period between mid-pregnancy and lactation. Mammary involution was an autolytic process aided by the action of macrophages accompanied by shrunken alveoli and increased interalveolar connective tissue. This feature was evident at 3 days after weaning which began from the periphery of the glands. The number of macrophages were abundant in the connective tissue and alveolar and ductal lumina. By day 8, the alveoli were no longer recognisable and at 12 days after weaning only remnants of ductules and alveoli were present. PERT-0332-1985 Communication I: Ganoderma boninense Pat. from Basal Stem Rot of Oil Palm (Elaeis guineensis) in Peninsular Malaysia Y.W. Ho Abstract Several hundred sporophores of Ganoderma were collected from 5-40 years old palm trees infected with basal stem rot in 5 oil palm estates in Peninsular Malaysia. Based on the morphometric studies of the pores, dessepiments and basidiospores dimensions and other morphological characteristics, the sporophores were identified as belonging to a single species, G. boninense Pat.