Risk assessment of chemical, laboratory experiment or
process
The risk assessment shall contain information about current chemicals, storage, handling,
preparation, use, end products, possible intermediate products and waste handling. Personal
security and external environment shall be taken into consideration.
Chemical/Laboratory experiment/Process
Testing of the recognition ability of Monoclonal mouse-anti-human β2-microgobulin
The aim of this laboratory exercise is to show students, by the use of a model system (Getting
Started Biacore 3000), how Biacore technology can be used for a bio-assay and more
specifically for the detection of an antigen using immobilesed antibodies. Furthermore the use of
sandwich assay for the signal amplification will be also shown.
Chemicals
Running buffer: (10 mM HEPES buffer + 150 mM NaCl + 3 mM EDTA + 0.005% Surfactant
P20)
Ligand: Monoclonal mouse-anti-human β2 microglobulin
Analyte: Human β2 microglobulin
Enhancement molecule: Polyclonal mouse-anti-human β2 microglobulin
Regeneration solution: 10% Glycerol
Area/Unit
Biosensors & Bioelectronics Centre
Laboratory/Premises
Biacore lab.
Risk assessment
Low risk
Moderately hazardous
Hazardous
Very hazardous
Inherent chemicals intrinsic hazardous properties (also end products and intermediate products if possible)
HEPES
May be harmful if inhaled, swallowed or when in contact with eyes.
NaCl
No significant hazard can be identified
EDTA
May be harmful if inhaled, swallowed or when in contact with eyes.
Glycerol:
May be harmful if inhaled, swallowed or when in contact with eyes.
Monoclonal/Polyclonal mouse-anti-human β2 microglobulin
Classified as not dangerous
Mouse-anti-human β2 microglobulin
Classified as not dangerous
Risks with storage, preparation, handling and waste handling shall be described at normal
conditions and regarding unexpected incidents.
Avoid contact with eyes, skin, and clothing.
Measures to limit risks (safety equipment, handling instructions, etc.)
The worker should use laboratory gloves, lab coat and tightly fit protective eyes glasses.
Environmental precautions
Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge
into the environment must be avoided.
Methods and materials for containment and cleaning up
Soak up with inert absorbent material and dispose of as hazardous waste. Keep in suitable,
closed containers for disposal.
First aid measures and measures in case of fire and waste
After inhalation
If inhaled, remove to fresh air.
After skin contact
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove
contaminated clothing and shoes. Call a physician.
After eye contact
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure
adequate flushing by separating the eyelids with fingers. Call a physician.
After ingestion
If swallowed, wash out mouth with water provided
Always report accident or a situation that could have led to an accident to the institution.
Risk assessment performed by:
Date
Valerio Beni
2014/01/20
Employers’ signature
Date