SUPPLEMENTARY MATERIAL 1 FST values for pairwise comparisons (below the diagonal) and geographic distance (km) (above the diagonal) between Galaxiella pusilla
populations in the west region. Non-significant differences in pairwise FST after corrections for multiple comparisons are indicated in bold*
1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
11
18
32
43
67
103
114
106
191
166
204
173
235
242
260
253
246
224
241
254
274
329
376
329
428
24
33
42
61
99
110
96
183
159
196
168
225
232
250
243
237
217
233
246
265
320
367
321
420
17
29
59
91
103
108
185
157
196
163
234
242
259
249
242
216
233
247
268
327
374
323
422
12
43
74
86
99
169
141
180
146
221
229
247
235
227
200
218
231
252
313
361
308
407
33
62
74
93
158
129
168
134
211
220
237
225
217
189
206
219
241
303
351
296
395
39
48
66
126
99
137
106
179
188
205
192
184
158
175
188
209
270
318
264
364
13
84
105
71
109
72
169
180
195
175
165
130
148
161
185
252
301
239
338
82
92
58
96
60
157
168
184
162
153
117
134
148
172
240
289
226
325
103
95
124
114
130
137
154
152
148
141
155
166
180
227
272
235
332
38
26
53
73
86
98
71
61
38
53
64
83
148
197
139
238
39
21
110
122
135
108
98
60
77
91
115
184
233
168
268
43
90
103
112
78
66
21
38
52
77
149
199
129
229
126
139
151
120
109
63
79
93
119
193
242
170
269
13
26
35
40
84
84
82
76
97
142
120
208
18
43
50
97
95
93
84
94
136
124
207
41
52
103
98
93
79
77
119
113
192
12
65
58
52
41
78
127
87
180
53
47
43
37
87
136
89
184
17
31
57
132
180
108
208
14
41
118
166
91
191
27
105
153
77
177
78
125
56
154
49
63
116
96
98
2
0.018
3
0.095
0.085
4
0.048
0.040
0.019
5
0.010
0.007
0.081
0.036
6
0.108
0.088
0.129
0.108
0.106
7
0.142
0.096
0.156
0.108
0.119
0.218
8
0.189
0.152
0.178
0.154
0.175
0.261
0.126
9
0.096
0.085
0.122
0.110
0.085
0.144
0.186
0.225
10
0.164
0.160
0.181
0.158
0.170
0.213
0.204
0.247
0.153
11
0.218
0.199
0.213
0.204
0.215
0.238
0.246
0.266
0.198
0.126
12
0.130
0.114
0.148
0.119
0.128
0.171
0.143
0.200
0.108
0.038
0.083
13
0.165
0.141
0.187
0.133
0.153
0.238
0.145
0.237
0.218
0.215
0.296
0.155
14
0.131
0.118
0.155
0.116
0.120
0.203
0.150
0.201
0.126
0.126
0.191
0.073
0.169
15
0.131
0.125
0.174
0.119
0.117
0.219
0.149
0.200
0.130
0.142
0.191
0.085
0.193
0.065
16
0.135
0.127
0.174
0.122
0.121
0.225
0.154
0.220
0.165
0.171
0.244
0.129
0.195
0.036
0.093
17
0.219
0.191
0.246
0.195
0.194
0.308
0.204
0.277
0.195
0.234
0.307
0.185
0.271
0.102
0.165
0.139
18
0.298
0.269
0.328
0.286
0.283
0.356
0.303
0.379
0.236
0.215
0.264
0.151
0.319
0.207
0.220
0.284
0.240
19
0.192
0.170
0.181
0.162
0.188
0.201
0.221
0.275
0.140
0.104
0.114
0.072
0.266
0.150
0.155
0.203
0.219
0.161
20
0.234
0.207
0.225
0.197
0.231
0.259
0.246
0.299
0.192
0.144
0.150
0.101
0.292
0.177
0.191
0.242
0.266
0.174
0.041
21
0.229
0.217
0.226
0.200
0.232
0.287
0.263
0.307
0.182
0.152
0.191
0.130
0.306
0.187
0.187
0.244
0.250
0.206
0.068
0.057
22
0.243
0.223
0.231
0.204
0.239
0.281
0.260
0.298
0.180
0.166
0.199
0.135
0.303
0.176
0.183
0.226
0.236
0.181
0.055
0.058
0.047
23
0.322
0.304
0.304
0.281
0.312
0.360
0.306
0.409
0.295
0.271
0.284
0.230
0.405
0.267
0.273
0.313
0.330
0.316
0.198
0.194
0.204
0.204
24
0.438
0.421
0.461
0.420
0.431
0.502
0.431
0.522
0.404
0.348
0.412
0.323
0.496
0.319
0.343
0.385
0.300
0.349
0.268
0.294
0.274
0.272
0.320
25
0.295
0.265
0.308
0.265
0.279
0.355
0.298
0.365
0.246
0.210
0.273
0.176
0.336
0.187
0.205
0.251
0.157
0.188
0.128
0.149
0.138
0.121
0.280
0.172
26
0.517
0.496
0.495
0.470
0.492
0.550
0.529
0.605
0.471
0.433
0.463
0.419
0.560
0.417
0.472
0.445
0.426
0.495
0.359
0.442
0.428
0.419
0.514
0.511
99
0.404
* Where 1=Bray Drain, 2=Reedy Creek Wilmont Drain, 3=Death Hole Outlet Drain, 4=Bevilaqua Drain, 5=Lake Bonney Drain, 6=The Claypans,7=Ewans Ponds, 8=Pick's Swamp, 9=Mosquito Creek, 10=Crawford River,
11=Fitzroy River, 12=Darlot Creek Tributary, 13=Bridgewater Lakes, 14=Tea Tree Creek, 15=Glenelg River, 16=Mount Rosea Creek, 17=Wannon River, 18=Boonawah Creek, 19=Eumeralla River, 20=Shaw River,
21=Moyne River, 22=Spring Creek, 23=Fiery Creek, 24=Mt Emu Creek Upper, 25=Mt Emu Creek Lower, 26=Gosling Creek
1
SUPPLEMENTARY MATERIAL 2 FST values for pairwise comparisons (below the diagonal) and geographic distance (km) (above the diagonal) between Galaxiella pusilla
populations in the east region. Non-significant differences in pairwise FST after corrections for multiple comparisons are indicated in bold*
27
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
12
15
17
26
34
26
17
56
57
73
38
114
128
172
179
201
207
227
264
149
356
379
429
266
12
16
27
34
24
6
47
47
63
26
104
118
161
169
191
197
218
255
137
344
368
417
260
5
15
22
12
18
57
57
71
31
113
126
168
177
199
206
228
265
140
348
368
419
251
11
18
8
22
61
61
75
35
117
130
172
182
204
211
233
270
143
351
371
421
249
7
7
33
72
71
84
44
127
139
180
191
213
220
242
279
148
357
373
425
241
11
40
77
77
89
48
131
143
184
195
217
224
247
284
149
358
372
425
235
29
66
65
78
37
120
133
173
184
206
213
236
273
141
350
367
418
240
40
41
57
22
98
111
155
163
185
191
212
249
133
339
364
413
263
4
21
31
59
72
117
123
146
151
172
209
107
306
339
385
277
18
29
57
71
115
122
144
150
171
209
103
303
335
382
274
41
42
55
98
106
129
135
158
195
87
286
319
364
271
83
95
137
147
169
176
199
236
111
318
342
391
251
14
60
65
87
93
117
154
74
252
296
337
292
46
52
74
81
106
143
70
239
285
325
296
27
38
54
83
116
65
193
245
282
302
23
31
59
94
91
202
262
295
328
20
48
79
102
182
249
278
338
29
63
119
194
265
292
356
37
148
202
281
303
385
181
196
285
300
416
209
232
280
237
103
103
367
61
314
28
0.182
29
0.301
0.175
30
0.308
0.171
0.278
31
0.151
0.158
0.164
0.235
32
0.335
0.176
0.132
0.253
0.154
33
0.478
0.308
0.232
0.418
0.276
0.107
34
0.505
0.484
0.438
0.506
0.305
0.432
0.573
35
0.486
0.504
0.433
0.554
0.277
0.441
0.581
0.212
36
0.442
0.427
0.373
0.471
0.237
0.374
0.507
0.051
0.101
37
0.600
0.602
0.571
0.644
0.446
0.569
0.684
0.234
0.310
0.198
38
0.437
0.451
0.392
0.488
0.244
0.396
0.530
0.070
0.079
0.032
0.201
39
0.547
0.532
0.512
0.587
0.398
0.511
0.604
0.536
0.494
0.461
0.592
0.449
40
0.509
0.505
0.483
0.549
0.354
0.487
0.597
0.503
0.461
0.431
0.568
0.412
0.227
41
0.566
0.603
0.620
0.648
0.518
0.626
0.722
0.659
0.644
0.603
0.679
0.597
0.643
0.617
42
0.461
0.505
0.492
0.558
0.370
0.523
0.623
0.477
0.446
0.395
0.530
0.404
0.445
0.415
0.569
43
0.387
0.408
0.381
0.442
0.275
0.398
0.504
0.405
0.356
0.331
0.444
0.327
0.307
0.249
0.491
0.169
44
0.470
0.469
0.433
0.495
0.300
0.433
0.526
0.425
0.381
0.354
0.498
0.338
0.183
0.097
0.590
0.332
0.161
45
0.559
0.569
0.502
0.613
0.400
0.538
0.652
0.524
0.480
0.442
0.596
0.430
0.398
0.325
0.683
0.337
0.178
0.177
46
0.537
0.548
0.521
0.596
0.362
0.523
0.654
0.484
0.441
0.398
0.572
0.382
0.332
0.258
0.667
0.353
0.224
0.142
0.264
47
0.510
0.512
0.517
0.583
0.409
0.559
0.655
0.550
0.567
0.495
0.615
0.481
0.556
0.522
0.604
0.562
0.461
0.505
0.630
0.611
48
0.648
0.646
0.623
0.723
0.512
0.667
0.775
0.711
0.701
0.644
0.790
0.629
0.688
0.625
0.794
0.683
0.592
0.599
0.746
0.755
0.627
49
0.437
0.390
0.421
0.416
0.326
0.436
0.549
0.461
0.486
0.416
0.538
0.434
0.551
0.508
0.591
0.519
0.424
0.455
0.569
0.539
0.535
0.585
50
0.426
0.430
0.438
0.496
0.316
0.483
0.590
0.519
0.515
0.456
0.601
0.457
0.567
0.524
0.577
0.467
0.413
0.486
0.581
0.547
0.521
0.675
0.409
0.277
0.379
0.446
0.501
0.302
0.477
0.637
0.603
0.584
0.526
0.686
0.518
0.622
0.588
0.613
0.505
0.429
0.538
0.672
0.646
0.568
0.811
0.473
51
374
0.463
* Where 27=Tributary of Dandenong Creek, 28=Golf Links Road Drain, 29=Eastern Contour Drain Tributary, 30=Boggy Creek, 31=Balcombe Creek, 32=Tuerong Creek, 33=Blackscamp Wetland, 34=Cardinia Creek
Tributary, 35=Dingo Creek Tributary, 36=Cannibal Creek, 37=King Parrot Creek, 38=Yallock Creek, 39=Moe Contour Drain, 40=Morwell River, 41=Deep Creek, 42=Merriman Creek, 43=Monkey Creek, 44=Flooding Creek,
45=Perry River, 46=Cobblers Creek, 47=Darby River , 48=Flinders Island, 49=Big Waterhouse Lake, 50=Icena Creek, 51=Harcus River
2
SUPPLEMENTARY MATERIAL 3 Two-dimensional plot showing the relationships among individuals of
Galaxiella pusilla based on a multivariate factorial correspondence analysis of 10 loci from (a) the west region and (b)
the east region. The first two factors are shown, with the percentage of variance explained in parenthesis. See Tables 1
& 2 for translation of site codes.
(a) West Region
3000
2500
Factor 2 (10.23%)
2000
1500
1000
500
0
-500
-1000
-1500
-1500
-1000
-500
0
500
1000
1500
Factor 1 (16.42%)
BRD
RCW
DEH
BEV
LBD
CLP
EWP
PIS
MOS
CRR
FIT
DLT
BRL
TTC
GLR
MRC
WAR
BOO
EUR
SHR
MOY
SPR
FIC
MEU
MEL
GOC
(b) East Region
2000
1500
Factor 2 (12.93%)
1000
500
0
-500
-1000
-4000
-3000
-2000
-1000
Factor 1 (15.40%)
3
0
1000
TDN
GLD
ECD
BOC
BAC
TUC
BCW
CAR
DIN
CAN
KPC
YAC
MOE
MOR
DEE
MER
MON
FLC
PER
COB
DAR
WIN
BWL
ICC
HAR
SUPPLEMENTARY MARTERIAL 4 Bayesian inference phylogenetic trees for Galaxiella pusilla haplotypes
generated from (a) 16S rDNA (16S), (b) cytochrome c oxidase subunit I (COI) and (c) Cytochrome b (CytB) fragments
of 482bp, 595bp, and 1083bp in size, respectively. The black-stripe minnow, Galaxiella nigrostriata, and common
galaxias, Galaxias maculatus, were used as outgroups. See Tables 1 & 2 for translation of site codes.
Comments on tree construction
For the construction of Bayesian inference phylogenetic trees, four Metropolis-coupled Monte Carlo Markov Chains
(MCMC) were run for 5,000,000 generations, and trees were sampled every 100 generations. A temperature parameter
of either 0.1 or 0.2 was applied to ensure that heated chains were all successfully contributing possible trees.
Convergence of runs was considered complete when the average standard deviation of split frequencies between
duplicate runs was less than 0.01. Stationarity of the chains was assessed by plotting log likelihood values against
generation time and observing the point where a stable mean equilibrium value was reached. Burn in values were
conservatively set to discard the first 1,250 trees (to remove trees outside the stable mean equilibrium) prior to
calculation of a consensus topology and estimates of posterior probabilities for 50% majority rule consensus trees.
Bayesian posterior probabilities greater than 0.95 were considered significant support for a clade (Hillis and Bull 1993;
Schultz et al. 2009).
References
Hillis, D.M., Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic
analysis. Systematic Biology, 42, 182–192.
Schultz MB, Smith SA, Horwitz P, Richardson AMM, Crandall KA, Austin CM (2009) Evolution underground: A
molecular phylogenetic investigation of Australian burrowing freshwater crayfish (Decapoda: Parastacidae) with
particular focus on Engaeus Erichson. Molecular Phylogenetics and Evolution, 50, 580–598
4
a)
16S
16S Hap6 (SHR)
16S Hap2 (FIC)
16S Hap13 (All other west region sequences)
100
16S Hap4 (BEV4)
16S Hap5 (BEV,DEH)
99
West
16S Hap1 (FIT)
92
99
16S Hap9 (CRR,DLT)
91
16S Hap8 (DLT2)
99
16S Hap10 (DLT4)
16S Hap7 (BRD6)
87
16S Hap15 (BWL,DAR,ECD1,HAR,ICC)
16S Hap11 (WIN)
100
East
16S Hap14 (PER)
16S Hap13 (All other east region sequences)
16S Hap12 (FLC2)
Galaxiella nigrostriata
Galaxias maculatus
0.005
5
b) COI
CO1 Hap1 (DLT7)
CO1 Hap35 (DLT4,9)
CO1 Hap36 (DLT1)
CO1 Hap25 (BOO,EUM,MOY,SHR,SPR)
CO1 Hap26 (EUM1,8)
79
91
CO1 Hap21 (CRR6,9,10)
CO1 Hap20 (CRR)
CO1 Hap11 (DLT)
60
CO1 Hap9 (FIT2,3,6)
Fitzroy
CO1 Hap10 (FIT)
98
CO1 Hap13 (GOC)
CO1 Hap2 (TTC05)
CO1 Hap3 (BRD4,8)
99
84
CO1 Hap5 (LBD3,10,RCW3)
CO1 Hap6 (BRD,CLP,LBD,RCW)
CO1 Hap7 (BEV7)
50
Bevilaqua
Death Hole
Fiery
CO1 Hap27 (BEV,DEH)
99
CO1 Hap32 (BEV1)
CO1 Hap4 (FIC4)
CO1 Hap8 (FIC)
99
Bray, Lake Bonney,
Claypans, Reedy Ck
West
CO1 Hap12 (TTC1,8)
CO1 Hap14 (MRC5,7)
CO1 Hap22 (GLR,MRC,TTC7)
60
CO1 Hap23 (MOS9)
82
CO1 Hap24 (MOS)
98
CO1 Hap16 (WAR)
Wannon
CO1 Hap15 (WAR5)
100
CO1 Hap17 (GLR3,7,8,MRC2,TTC)
CO1 Hap18 (BRL4)
CO1 Hap19 (BRL)
CO1 Hap28 (MEU)
CO1 Hap29 (MEL)
86
CO1 Hap30 (MEL9)
78
CO1 Hap31 (MEL8)
Port Phillip
Western Port
CO1 Hap33 (EWP,PIS)
86
CO1 Hap34 (PIS9)
CO1 Hap37 (YAC4)
95
CO1 Hap38 (BAC,BCW,BOC,CAN,CAR,DIN,ECD,GLD,KPC,TDN,TUC,YAC)
CO1 Hap39 (KPC4)
CO1 Hap40 (MOR1,4,11)
CO1 Hap48 (FLC8,9)
74
CO1 Hap50 (COB,FLC,MOE,MOR)
CO1 Hap41 (MON1)
CO1 Hap42 (WIN)
100
53
CO1 Hap44 (BWL,ICC)
98
CO1 Hap47 (BWL5)
CO1 Hap45 (HAR)
97
CO1 Hap46 (DAR)
CO1 Hap43 (PER)
CO1 Hap49 (FLC2)
Galaxiella nigrostriata
Galaxias maculatus
0.02
6
East
CO1 Hap51 (DEE,MER,MON)
71
Wilsons
Promontory
Tasmania
c) CytB
CYTB Hap72 (FIC9)
CYTB Hap70 (FIC5)
CYTB Hap69 (FIC1)
CYTB Hap71 (FIC)
CYTB Hap68 (FIC7)
CYTB Hap82 (GOC)
CYTB Hap77 (MEL10,11)
100
CYTB Hap76 (MEL)
89
Mt Emu
CYTB Hap75 (MEU)
CYTB Hap58 (DEH8)
CYTB Hap43 (BEV,DEH)
CYTB Hap49 (RCW2)
CYTB Hap47 (CLP)
CYTB Hap46 (LBD4)
CYTB Hap45 (BRD10,LBD1,RCW6,7,8)
100
100
South
Australia
CYTB Hap44 (BEV7)
CYTB Hap81 (BRD2,5,9)
73
99
CYTB Hap80 (RCW5)
CYTB Hap79 (BRD,LBD,RCW1,3,10,11)
CYTB Hap48 (BRD7)
93
CYTB Hap42 (BRD3,LBD5,7,RCW9)
CYTB Hap41 (DEH5)
CYTB Hap40 (DEH6)
West
CYTB Hap39 (WAR2)
100
Wannon
CYTB Hap38 (WAR)
CYTB Hap33 (BRL)
CYTB Hap37 (EWP1)
9694
Bridgewater
Ewans, Picks
CYTB Hap36 (EWP10)
CYTB Hap35 (EWP2,9)
100
87
CYTB Hap34 (PIS)
CYTB Hap29 (EWP)
CYTB Hap56 (BOO,MOY,SPR)
93
CYTB Hap55 (EUM)
CYTB Hap54 (EUM39)
98
91
Eumeralla
CYTB Hap53 (EUM35)
CYTB Hap32 (MOY31)
CYTB Hap31 (SHR)
CYTB Hap30 (MOY32,36,40,SHR31)
98
CYTB Hap66 (FIT4)
90
100
CYTB Hap63 (FIT)
Fitzroy
CYTB Hap74 (FIT1)
99 CYTB Hap59 (FIT7)
CYTB Hap64 (DLT31,37,39)
CYTB Hap65 (CRR36,39,40)
73
95
CYTB Hap67 (CRR32)
Crawford
CYTB Hap62 (CRR)
100
CYTB Hap78 (DLT33)
91
86
CYTB Hap61 (DLT38)
CYTB Hap60 (DLT35,36,40)
96
CYTB Hap73 (DLT34)
CYTB Hap28 (DLT32)
92
CYTB Hap51 (MOS)
Grampians
Mosquito
CYTB Hap57 (GLR35)
100
CYTB Hap52 (MRC7)
CYTB Hap50 (GLR,MRC,TTC)
71
CYTB Hap27 (GLR31,32,40,MRC1,8,10,TTC7)
CYTB Hap20 (HAR)
CYTB Hap26 (ICC)
100
CYTB Hap25 (WIN5)
100
Tasmania
Wilsons
Promontory
CYTB Hap24 (WIN8,9,10)
CYTB Hap23 (WIN3)
88
100
CYTB Hap22 (WIN)
CYTB Hap21 (BWL4,6)
CYTB Hap19 (BWL)
99
CYTB Hap18 (BWL2)
CYTB Hap17 (BWL8)
CYTB Hap16 (DAR39)
East
CYTB Hap1 (DAR)
97
91
CYTB Hap15 (DEE)
Deep
CYTB Hap14 (DEE2,3,6,7)
CYTB Hap13 (MER)
100
57
CYTB Hap12 (FLC1,3)
CYTB Hap11 (COB36)
CYTB Hap10 (COB,FLC,MOE,MON,MOR)
CYTB Hap9 (MOE31,35,41)
CYTB Hap7 (PER)
CYTB Hap8 (BOC3,6,11)
CYTB Hap6 (TDN)
Port Phillip
Westernport
CYTB Hap5 (CAN11)
98
CYTB Hap4 (CAR39)
CYTB Hap3 (GLD9)
CYTB Hap2 (BAC,BCW,BOC,CAN,CAR,DIN,ECD,GLD,KPC,TDN3,6,10,TUC,YAC)
Galaxiella nigrostriata
Galaxias maculatus
0.02
7
SUPPLEMENTARY MATERIAL 5 Analysis of demographic history of Galaxiella pusilla populations, where
populations are grouped according to east and west regions. Significant values after correction for multiple comparisons
are in bold font.
N
Tajima’s
D
Pvalue
Mismatch
Growth-Decline
Raggedness
index r
P-value
Fu &
Li’s F*
P-value
Fu &
Li’s D*
P-value
Fu’s Fs
P-value
16S
10
-1.0143
0.1850
0.1027
0.2896
-1.1288
0.1900
-1.0008
0.1940
-9.412
<0.001
COI
36
-0.8711
0.1780
0.0073
0.0080
-0.4273
0.3250
-0.0964
0.4400
-37.057
<0.001
CytB
56
-1.1396
0.1140
=0,1000
2ut=2.4444
=2.44492,1000
2ut=5.9662
=4.37590,1000
2ut=10.1676
0.0034
<0.0001
-1.9822
0.0370
-1.9751
0.0330
-34.117
<0.001
16S
5
-0.4102
0.4440
0.2400
0.4625
-0.4175
0.4340
-0.4102
0.4310
-3.304
0.035
COI
15
-0.8235
0.2180
0.1005
0.6356
-1.1297
0.1610
-1.0428
0.1450
-12.153
<0.001
CytB
27
0.1560
0.6270
=0,1000
2ut=1.8
=1.10567,1000
2ut=3.8086
=4.81871,1000
2ut=5.9904
0.0116
0.0440
0.0295
0.5280
-0.0365
0.4280
-16.201
<0.001
WEST
EAST
8
SUPPLEMENTARY MATERIAL 6 Two-dimensional plot comparing genetic diversity (mean number of alleles and
heterozygosity) to genetic uniqueness (mean Cytochrome b Kimura-2-parameter distance) for each Galaxiella pusilla
population within the west region (a) and (b) and the east region (c) and (d) . See Tables 1& 2 for translation of site
codes
(a)
(b)
0,018
R² = 0,0981
0,018
0,017
0,016
0,016
0,015
0,015
Mean CytB K2P
Mean CytB K2P
0,017
0,014
0,013
0,012
R² = 0,1513
0,014
0,013
0,012
0,011
0,011
0,010
0,010
0
2
4
6
8
Mean No. Alleles
0,1
10
(c)
0,3
0,5
Heterozygosity (HE)
0,7
(d)
0,013
0,013
R² = 0,1845
0,012
R² = 0,2569
0,012
0,011
Mean CytB K2P
Mean CytB K2P
0,011
0,010
0,009
0,010
0,009
0,008
0,008
0,007
0,007
0,006
0,006
1
2
3
4
Mean No. Alleles
5
0
9
0,1
0,2
0,3
0,4
Heterozygosity (HE)
0,5